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All Journal Jurnal Pendidikan Biologi Undiksha Jurnal Biologi dan Pembelajarannya (JBP) Wahana-Bio: Jurnal Biologi dan Pembelajarannya Jurnal Pembelajaran dan Biologi Nukleus Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) BERDAYA: Jurnal Pendidikan dan Pengabdian Kepada Masyarakat Konservasi Hayati BERNAS: Jurnal Pengabdian Kepada Masyarakat Lumbung Inovasi: Jurnal Pengabdian Kepada Masyarakat Indonesian Journal of Medical Chemistry and Bioinformatics Maximus: Journal of Biological and Life Sciences Bioeksperimen Jurnal Bioteknologi & Biosains Indonesia (JBBI) Indonesian Journal of Jamu Jurnal Bioteknologi & Biosains Indonesia
Khaerunissa Anbar Istiadi
Program Studi Biologi, Institut Teknologi Sumatera, Lampung Selatan, Lampung, Indonesia 35365
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Dentistry Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Mathematics Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Veterinary Other

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Isolation and screening of halotolerant protease-producing bacteria from Lampung fermented shrimp paste Istiadi, Khaerunissa Anbar; Ningsih; Suryanti, Erma; Imaniar, Lisana Husna
Bioeksperimen: Jurnal Penelitian Biologi March 2026
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v12i1.14593

Abstract

Protease are class of degradative enzymes that hydrolyze proteins, breaking its peptide bond, into smaller peptides and amino acids. Microbial proteases have gained increasing attention due to its productivity and utilization in various industries. Proteolytic bacteria derived from fermented shrimp paste from Lampung have great potential to meet this need. However, studies on these bacteria are still limited, so further research is needed to explore and optimally utilize their potential. This study aims to explore the diversity of proteolytic bacteria from Lampung terasi.. The methods employed included isolation stages using Nutrient Agar supplemented with 5% and 10% NaCl (w/v), followed by proteolytic screening properties using Skim Milk Agar media. A total of 20 bacterial isolates were obtained from terasi using Nutrient Agar supplemented with 5% (w/v) NaCl, and 15 isolates were obtained from Nutrient Agar (NA)  supplemented with 10% (w/v) NaCl. Screening results showed that 85% and 67% of the isolates obtained from NA supplemented with 5% and 10% NaCl, respectively, exhibited proteolytic activity. Halotolerant bacterial isolates isolated using NA + 5% NaCl exhibited a proteolytic index ranging from 0.3 – 3.0, while those isolated with NA + 10% NaCl showed values ranging from 0.18 to 3.06. The screening results revealed that isolates BTCL5-07 and BTCL10-05 exhibited the highest proteolytic activity, with proteolytic indices of 3.0 and 3.06, respectively. These findings indicate that halotolerant bacteria from Lampung terasi represent a promising source of potential proteolytic enzymes. Further research is required to optimize enzyme production.
Co-Authors Aji Hafidz Talaga Alawiyah, Alawiyah Alfauzia, Nadia Amelia, Tiara Andreas Dwi Nugroho Andriani, Silvia Aniesti, Frigia Rafilia Anisa, Hida Arliani Nur Ariyanto, Dika Ariyanto Astuti, Ayu Widia Desi Budiono Deviany Deviany, Deviany Dewi Chusniasih DINA ANGGRAINI Dini Meilinda Dwi Anita Suryandari Erma Suryanti Fajri Arif Wibawa Fatimatuzzahra, Izza Fatriani, Rizka Febrina, Yane Fita Feerzet Achmad Ferdinanda, Thierry Garbono, Andrian Gres Maretta, Gres Hadi Sunaryo Handayani, Catie Hariyandi, Yopi Hida Arliani Nur Anisa Iffa Afiqa Khairani Imaniar, Lisana Husna Irandi Putra Pratomo Jannah, Umi Nur Luluk Yunaini Muhyi, Abdul Mulyadi, Salsa Pratiwi Mulyana, Jeane Siswitasari Nadia Rahmah, Nadia Ningsih NINGSIH, NINGSIH Nur Anisa, Hida Arliani Puji Sari Reni Yuniarti Restiawati, Nawang Sari, Dian Anggria Satria, Arysca Wisnu Siragih, Rajuman S Suryanti, Erma Triya Yuli Andini Yuni Sukmawati Yusuf, Reggina Aulia Zhofiroh, Nawrah Meisyah Muthi'ah