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Journal : Research Journal of Life Science

Investigation of Scutellaria radix on Phenoloxidase and Prophenoloxidase of Giant Freshwater Prawn, Macrobrachium rosenbergii, via Reverse-gavage Feeding Andriawan, Soni; Bao, Hung Tran; Cheng, Phillip T.; Yuniarti, Ating
Research Journal of Life Science Vol 5, No 3 (2018)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (346.662 KB) | DOI: 10.21776/ub.rjls.2018.005.03.7

Abstract

Scutellaria radix is one of the ancientest approaches to treat diseases. The aim of this study was to investigate the effect of Scutellaria radix on the immune responses of Macrobrachium rosenbergii by reverse-gavage feeding and their protection against Lactococcus garvieae. In brief, M. rosenbergii was injected with S. radix water extract at 1, 5 and 10 µg/µl via reverse-gavage feeding (RGF). Phenoloxidase (PO) activity of hemocytes and prophenoloxidase (proPO) gene of hepatopancreas were assessed to determine M. rosenbergii immunity. The results revealed that the concentration of S. radix extract at 5 µg/µl enhanced significantly the phenoloxidase of M. rosenbergii correlated with the control group. For pro PO were up-regulated by all concentration in a time-dependent manner. The highest percentage of survival rates was observed at 5 µg/µlof S. radix water extract after challenged with L. garvieae. The present discoveries suggest that S. radix water extract at the concentration of 5 µg/µlmay raise the immune responses and gene expressions in M. rosenbergii using RGF.
Minimum Inhibitory Concentration of Marine Microalgae Dunaliella salina on Fish Pathogenic Bacteria Edwardsiella tarda Rusmawanto, Rusmawanto; Prajitno, Arief; Yuniarti, Ating
Research Journal of Life Science Vol 6, No 2 (2019)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (353.624 KB) | DOI: 10.21776/ub.rjls.2019.006.02.1

Abstract

Dunaliella salina is a type of marine microalgae. The objective of this research were investigated the effect of D. salina to inhibit the growth of E. tarda. The method used in this study was examined antibacterial activity of D. salina using disc diffusion and co culture test. The secondary metabolite compound in D. salina was tested using phytochemical screening and FTIR methods. The results obtained D. salina extracted using n-hexane showed the best activity for inhibiting the growth of E. tarda. Minimum concentration of 100 mg.L-1 crude extract can inhibit E. tarda with a total of bacterial colonies 137x1011 CFU.mL-1. The phytochemical test results showed that D. salina extract using n-hexane contained flavonoids, saponins, alkaloids, terpenoids and phenols. The FTIR analysis showed phenol and carotene compounds are one of the secondary metabolites that can be used as antibacterial. It can be concluded that the extract has strong antibacterial activity against E. tarda and potentially as antibacterial in aquaculture.
Chicken Feather Silage Meal As A Fish Meal Protein Source Replacement In Feed Formula Of Pomfret (Colossoma macropomum) Arning Wilujeng Ekawati; Ating Yuniarti; Marsoedi Marsoedi
Research Journal of Life Science Vol 3, No 2 (2016)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (569.949 KB) | DOI: 10.21776/ub.rjls.2016.003.02.4

Abstract

The research was conducted to know the effect and to determine the best utilization of the chiken feather silage meal as a substitute for fish meal protein source in the feed formula of Colossoma macropomum. This study used experimental method based on Completely Randomized Design (CRD) with 5 treatments and 3 replications. Five diets (33% isoprotein and 3.85 kcal/g diet isoenergi) were formulated with substitution of the chiken feather silage meal to fishmeal protein. These substituted 0, 25, 50, 75 and 100 % (A, B, C, D and E respectively) of the fishmeal protein. Parameters observed and analyzed were: survival rate, specific growth rate, feed conversion ratio, protein efficiency ratio, protein retention and protein digestibility. The results showed that the treatment had no effect on survival rate, specific growth rate, feed conversion ratio, protein efficiency ratio, and protein retention but the effect on protein digestibility. Based on these results, it can be concluded that fishmeal protein can be substituted with the chiken feather silage meal up to 100% in the feed formula of Colossoma macropomum.
The Effect of Ulva lactuta Polysaccharide Extract on Total Haemocyte Count and Phagocytic Activity of L. vannamei Suleman Suleman; Sri Andayani; Ating Yuniarti
Research Journal of Life Science Vol 5, No 3 (2018)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (28.393 KB) | DOI: 10.21776/ub.rjls.2018.005.03.3

Abstract

This research aimed to evaluate the effect of polysaccharide extract of Ulva lactuta on the total haemocytes count (THC) and the phagocytic activities (PA) on Litopenaeus vannamei. The concentration of the extract used in this research was 5 ppm and it was applied through the immersion. The haemolymph was collected after the administration in the different periods at hour of 4 and 24. The results showed that the administration of polysaccharide extracts increased THC and PA in L. vannamei significantly (p<0.05). THC and PA of white-leg shrimp with polysaccharide extract was higher than that without the administration of the extract. The enhancement of THC was found after the administration at hour 4 from 5.00 cells. ml-1 to 5.26 x 104 cells. ml-1. However, the THC level decreased after 24 hours at the value of 5.19 x 104 cells. The increasing pattern was also true for PA of L. vannamei, from 40.44% to 53.67% (hour 4). At the hour of 24, the PA was found decreased to the level and then decreased 45.67 ±1.53. On the contrary, there were no enhancement of THC and PA in the treatment without polysaccharide extract of Ulva lactuta.
Co-Authors ., Suleman A.P.W Marhendra Abdul Hamid A. Toha Abdul Rahem Faqih Agung Setia Abadi Amrulloh, Albazi Achmad Anam, C Andriawan, Soni Anik Hartinah Mariati Anik M. Hariati Annisa Farhana Dewi Apriliyanti, Fisma Josara Arief Prajitno Arief Prajitno Arief Prajitno, Arief Arifin, Nasrullah Bai Ariyani, Destia Fitri Arning Wilujeng Ekawati Aulia Rahmawati, Aulia Bao, Hung Tran Budianto Budianto C Anam Cheng, Phillip T. Citra Satrya Utama Dewi Dewa Gede Raka Wiadnya Dewa Gede Raka Wiadnya Dewi, Citra Satrya Utama Diana Permatasari Eka Supriatin, Febriyani Endang Yuli Herawati Happy Nursyam Hariati, Anik Martina Hariati, Anik Martinah Kholil, Kiki Nur Azam Kusuma, Wahyu Endra Lailaturramadhini, Nova Lalu Panji Imam Agamawan Lestari, Bela Fatma Hani Ayu M. Indra Wahyu Pratama Mahariawan, I Made Dedi Maheno Sri Widodo, Maheno Sri Marhendra, A.P.W Marsoedi Marsoedi Maulana, Antajala Rian Mayor, Corazon Ell Merilia Papuani Milansari, Sindi Mohamad Fadjar Muammar Kadhafi Muhammad Dailami, Muhammad Muhammad Fakhri Muhammad Fakhri Muhammad Fakhri Nasrullah Bai Arifin Nasrullah Bai Arifin Nasrullah Bai Arifin Nurcahyani, Shinta Eka Pangestu, Lintang Dion Paricahya, Akhsan Fikrillah Pratama, Rakhmad Ndaru Putri, Radwa Fathin H Ramadhan, Surya Dewa Rani Yuwanita Ridwanudin, Asep Rika Putri Rusmawanto, Rusmawanto Saleky, Dandi Septi Anitasari, Septi Septiana Sri Astuti, Septiana Sri Siahaan, Marthin Daniel Theo Gratia Sianturi, Andre Soaloon Sitti Khairul Bariyyah Sri Andayani Sri Andayani Sri Andayani Sufaichusan, Ifa Sukandar Sukandar Suleman Suleman Supriatin, Febriyani Eka Suryanto, Damang Yunita Maimunah