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All Journal HAYATI Journal of Biosciences Jurnal Ilmu dan Teknologi Kelautan Tropis Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Biota Jurnal Perlindungan Tanaman Indonesia Journal of Tropical Biodiversity and Biotechnology BERITA BIOLOGI Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Jurnal Perikanan Universitas Gadjah Mada JMM (Jurnal Masyarakat Mandiri) Indonesian Journal of Biology Education Biota Journal of Aquaculture and Fish Health JURNAL PERIKANAN TROPIS Borneo Journal of Medical Laboratory Technology Berkala Penelitian Hayati Biological Environment and Pollution Rumphius Pattimura Biological Journal Berkala Ilmiah Biologi
Bambang Retnoaji
Fakultas Biologi Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Energy Environmental Science Health Professions Immunology & microbiology Library & Information Science Materials Science & Nanotechnology Medicine & Pharmacology Social Sciences Veterinary Other

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The effect of Garcinia mangostana L. skin decoct exposure on Zebrafish embryo Danio rerio (Hamilton, 1822) Yunita, Neri; Retnoaji, Bambang
Biological Environment and Pollution Vol. 3 No. 1 (2023)
Publisher : Association for Scientific Computing, Electronics, and Engineering (ASCEE)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31763/bioenvipo.v3i1.631

Abstract

Hypertension is one of the diseases with the highest prevalence in Indonesia and is the main cause of death in patients. Pregnant women have a risk of losing their baby due to hypertension or death. ACEI and ARB-type antihypertensive drugs are dangerous for pregnant women because they can cause fetal death. In ancient times, Indonesians have used plants as herbal medicines for generations. One of the plants that has medicinal properties is mangosteen skin (Garcinia mangostana L.) as an antihypertensive drug through its diuretic effects. However, the drugs consumed can have toxic effects if used in high concentrations. A teratogenic test is a toxic test that is specifically carried out to determine the presence of organ abnormalities in the fetus due to exposure to xenobiotic compounds. The zebrafish (Danio rerio) embryo was chosen as the research object because the chorion in the embryonic phase is transparent, so it is easy to observe. This study aimed to determine the effect of exposure to mangosteen rind decoction on zebrafish embryos' hatchability and spinal curvature. Observations were made using a Leica microscope with ANOVA data analysis. The observations showed that high treatment concentrations decreased heart rate, while high concentrations didn’t affect egg hatching rate, spine curvature, or pigment density.
Histopathological Effects of Mangosteen (Garcinia mangostana L.) Peel Decoction on Betta Fish (Betta sp.) Liver Ariesti, Wiwin; Aeniah, Siti; Halim, Shuha Ma’muriyah; Sofyantoro, Fajar; Wijayanti, Nastiti; Retnoaji, Bambang; Nuriliani, Ardaning; Saragih, Hendry T.S.S.G.; Rohmah, Zuliyati; Widiyanto, Slamet; Pusparini, Nur Ainun Oktavia; Empra, Desi Eka Putri; Septriani, Nur Indah
Biota Vol 17 No 2 (2024)
Publisher : Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Mangosteen (Garcinia mangostana L.) peel contains bioactive compounds known for their health benefits, yet potential toxicity at certain doses remains a concern. This study evaluates the histopathological effects of mangosteen peel decoction on the liver of Betta fish (Betta sp.), a sensitive model organism. Mangosteen peel decoction was prepared and administered to Betta fish at concentrations of 5, 25, and 50 ppm, with a control group receiving no treatment. Fish were observed for changes in swimming activity and appetite over five days. Liver tissues were collected, processed, and analyzed histologically to assess tissue damage including vacuolization, pyknosis, hemorrhage, and necrosis. Data were analyzed using the Kruskal-Wallis and Mann-Whitney tests. Behavioral analysis indicated a dose-dependent reduction in swimming activity and appetite in treated groups. Histopathological examination revealed significant liver damage across all treatment groups, with higher concentrations of decoction correlating with increased hemorrhage, pyknosis, and necrosis. Vacuolization was highest in the control group and lowest in the 50-ppm group. The overall hepatic damage was categorized as moderate, with the control group showing the least damage. Mangosteen peel decoction induced significant hepatic damage in Betta fish, highlighting the cytotoxic effects at higher doses. The observed behavioral and histopathological changes underscore the need for careful consideration of decoction concentrations to avoid adverse effects in aquatic organisms. This study provides crucial insights into the toxicological impacts of mangosteen peel decoction on fish liver health, emphasizing the importance of dose regulation in practical applications. Further research is recommended to explore protective measures and alternative treatments to mitigate liver damage.
Natural Dye as an Alternative to Hematoxylin-Eosin Staining on Histological Preparations Karlina, Ina; Pusparini, Nur Ainun Oktavia; Maharesi, Chesa Ekani; Saeed, Faisal; Retnoaji, Bambang; Saragih, Hendry; Septriani, Nur Indah; Rohmah, Zuliyati; Hadi, Susilo; Nuriliani, Ardaning
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 2 (2025): June 2025
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i2.7909

Abstract

Hematoxylin-eosin is widely utilized in the field of animal microtechniques. However, the need to develop alternative dyes from natural sources such as plants has gained attention. Several studies have shown that many plants contain secondary metabolites with the potential to be developed as natural dyes. Lonchocarpus cyanescens and Syzygium cumini are promising candidates as alternative dyes for hematoxylin, while Lawsonia inermis and Hibiscus sabdariffa have shown potential as substitute dyes for eosin. These plants contain various secondary metabolites, including anthocyanins, flavonoids, chlorophyll, betalains, alkaloids, saponins, tannins, carbohydrates, proteins, phenolics, terpenoids, quinones, coumarins, xanthones, and resins. L. cyanescens exhibits a strong binding affinity to cells and tissues, particularly testicular tissue. Dyes derived from Syzygium cumini have been shown to provide a good staining result for rat liver cells. In contrast, dyes from Lawsonia inermis can stain cytoplasmic components and muscle fibers. Additionally, the dye from Hibiscus sabdariffa is capable of staining various biological components, including sperm, nerve cells, and blood cells. The dye preparation process involved extraction from different plant organs, such as leaves, flowers, and fruit. These findings suggest that secondary metabolites from these four plants hold significant potential for development as natural dyes to replace hematoxylin-eosin in histological applications.
Osteological Development of Yellow Rasbora Fish Rasbora lateristriata (Bleeker, 1854) Treated with Mangosteen Garcinia mangostana L. Peel Simplicia Paramita, Pradnya; Khasanah, Luthfia Uswatun; Retnoaji, Bambang
HAYATI Journal of Biosciences Vol. 32 No. 6 (2025): November 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.6.1663-1673

Abstract

Mangosteen peel simplicia (Garcinia mangostana L.) contains potential ingredients used in medicine. A toxicity test of mangosteen peel simplicia is needed before it is used. Wader pari (Rasbora lateristriata) is a potential animal model for toxicity testing. Thus, this study aims to determine the effect of mangosteen peel extract at various concentrations on the behavior and bone structure of R. lateristriata. In this research, fish behavior was observed at 48 hpf (hours post-fertilization) and 30 dpf (days post-fertilization). Bone structure observed at 96 hpf, 7 dpf, 14 dpf, 21 dpf, 28 dpf, 35 dpf, and 42 dpf using the Alizarin Red ‒ Alcian Blue method. Observation data on behavior and bone structure were analyzed descriptively, while the number of vertebrae was analyzed quantitatively using One-Way ANOVA (P < 0.05). The results showed that the ossification of cranium and caudal complex skeleton components in the 1 and 5 μg/mL treatments was significantly behind that of the control and 0.5 μg/mL treatments. Furthermore, 5 μg/mL treatment caused some behavioral and swimming pattern abnormalities in 48 hpf larvae. In conclusion, exposure to 1 and 5 μg/mL mangosteen peel simplicia resulted in skeletal growth inhibition in R. lateristriata. Exposure to 5 μg/mL mangosteen peel simplicia caused abnormalities in the behavior and swimming pattern of 48 hpf larvae.
The Use of OODEV® in Improving the Reproductive Performance of Male Yellow Rasbora Fish Rasbora lateristriata (Bleeker, 1854) Hurul Ein; Retnoaji, Bambang; Slamet Widiyanto
Journal of Aquaculture and Fish Health Vol. 13 No. 2 (2024): JAFH Vol. 13 No. 2 June 2024
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v13i2.46975

Abstract

A local commodity, Yellow rasbora fish Rasbora lateristriata (Bleeker, 1854), can potentially be used as a food resource by the Kulon Progo community. Added to that, cultivation or aquaculture is known to optimize this potential. Furthermore, Aquaculture will also prevent illegal fishing in nature, where yellow rasbora fish are listed as vulnerable species in the IUCN Red list. This study induced male yellow rasbora fish using the hormone OODEV® injection, which comprises the hormone Pregnant Mare Serum Gonadotropin (PMSG) and anti-dopamine. PMSG is a hormone that contains FSH as well as LH in it. Yellow rasbora fish were nurtured to the age of six months and treated with four different treatments (dose) for a month, namely 0 mL/Kg, 0.25 mL/Kg, 0.5 mL/Kg, and 0.75 mL/Kg. Observed parameters included motility (movement and duration) as well as sperm count, sperm viability, sperm morphology, sperm morphometry, gonadosomatic index (GSI), and survival rate (SR). Data obtained were analyzed using SPSS with the One-way ANOVA test and continued with Duncan's test if the results were significant. The obtained results had no significant effect on the IGS value (p>0.05), with the highest value found at a dose of 0.75 mL/Kg. In addition, administration of OODEV® also improved sperm quality with the best results at 0.75 mL/Kg  in terms of motility with the highest progressive movement compared to other doses but not significant  (p>0.05) compared to the control,  and significantly (p<0.05) longest movement duration compared to other doses, while in terms of morphometry it had the second widest middle piece area but not significant (p>0.05) compared to other doses, widest tail area but not significant (p>0.05) compared to other doses except with 0.25 mL/Kg, longest tail length  (p>0.05) but not significant with other doses except with 0.25 mL/Kg , and significantly (p<0.05) had the longest sperm total length. Every dose showed a 100% percentage of survival rate.
Natural Dye as an Alternative to Hematoxylin-Eosin Staining on Histological Preparations Karlina, Ina; Pusparini, Nur Ainun Oktavia; Maharesi, Chesa Ekani; Saeed, Faisal; Retnoaji, Bambang; Saragih, Hendry; Septriani, Nur Indah; Rohmah, Zuliyati; Hadi, Susilo; Nuriliani, Ardaning
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 2 (2025): June 2025
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i2.7909

Abstract

Hematoxylin-eosin is widely utilized in the field of animal microtechniques. However, the need to develop alternative dyes from natural sources such as plants has gained attention. Several studies have shown that many plants contain secondary metabolites with the potential to be developed as natural dyes. Lonchocarpus cyanescens and Syzygium cumini are promising candidates as alternative dyes for hematoxylin, while Lawsonia inermis and Hibiscus sabdariffa have shown potential as substitute dyes for eosin. These plants contain various secondary metabolites, including anthocyanins, flavonoids, chlorophyll, betalains, alkaloids, saponins, tannins, carbohydrates, proteins, phenolics, terpenoids, quinones, coumarins, xanthones, and resins. L. cyanescens exhibits a strong binding affinity to cells and tissues, particularly testicular tissue. Dyes derived from Syzygium cumini have been shown to provide a good staining result for rat liver cells. In contrast, dyes from Lawsonia inermis can stain cytoplasmic components and muscle fibers. Additionally, the dye from Hibiscus sabdariffa is capable of staining various biological components, including sperm, nerve cells, and blood cells. The dye preparation process involved extraction from different plant organs, such as leaves, flowers, and fruit. These findings suggest that secondary metabolites from these four plants hold significant potential for development as natural dyes to replace hematoxylin-eosin in histological applications.
Review: Aplikasi Histopatologi Untuk Praktik Forensik: Review: Applications of Histopathology for Forensic Practice Faulana, Arum Fahmi; Empra, Desi Eka Putri; Rahmadian, Desti; Shalihah, Fianicha; Nuriliani, Ardaning; Retnoaji, Bambang; Saragih, Hendry Tri Sakti SG; Rohmah, Zuliyati; Septriani, Nur Indah
Borneo Journal of Medical Laboratory Technology Vol. 6 No. 2 (2024): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v6i2.6582

Abstract

Forensics plays an important role in investigating crimes because it can reveal everything related to a murder case, including the time and cause of the murder. Histopathology is the study of damage to body tissues and organs which can be used to help investigate crimes. The aim of this literature review is to study the role of histopathology applications for forensic practice. The method used is searching journals and books using Google Scholar, ScienceDirect, NCBI, and PubMed with the keywords histopathology, forensics, diagnosis, death, microscopy, disease, staining, and autopsy. The histology approach helps the pathologist make a clear determination on the cause of death. The aim of histopathology is to perfect the macroscopic diagnosis and act as an additional examination in forensic case management. One of the histopathology methods in the forensic field is the paraffin method with hematoxylin eosin staining and special stains, immunohistochemistry, and MITS. The parameters observed with this method are measurements of pulmonary fat embolism; determining the age of myocardial infarction; assessment of liver steatosis, fibrosis, and cirrhosis; determination of the age of thrombus or thromboembolism; and diagnosis of shock with various diseases. The prospect of forensic histopathology is to complement molecular diagnostic methods, so that the combination of molecular and histology methods can play a maximum role in the field of forensic pathology. The conclusion of this literature review is that histopathology can be used to confirm the diagnosis in forensic cases and has good future prospects if combined with other methods such as molecular.
Modulatory Effects of Fucoidan on Paracetamol-induced Hepatic Damage and Histological Alterations in Rasbora lateristriata Sofyantoro, Fajar; Dafa, Maula Haqul; Primahesa, Alfian; Oktaviana, Shintia; Mayani, Olvita; Simanungkalit, Eben Ezer; Prissandi, Anthera Al Firdaus; Nuriliani, Ardaning; Retnoaji, Bambang; Saragih, Hendry T. S. S. G.; Rohmah, Zuliyati; Widiyanto, Slamet; Wijayanti, Nastiti; Septriani, Nur Indah
Biota Vol 12 No 1 (2026): Jurnal Biota 2026
Publisher : Faculty of Science and Technology Universitas Islam Negeri Raden Fatah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19109/biota.v12i1.27845

Abstract

Paracetamol overdose is a well-documented cause of hepatic injury across vertebrate species, including teleost fish. This study aimed to evaluate the hepatoprotective potential of fucoidan, a sulfated polysaccharide derived from brown algae, against paracetamol-induced liver damage in Rasbora lateristriata. Fish were divided into five experimental groups and exposed to paracetamol (3 mg/L), either alone or in combination with fucoidan at concentrations of 50, 100, or 300 µg/mL, for seven days. Histopathological evaluation of liver tissues was performed using hematoxylin–eosin staining, with semi-quantitative scoring focused on hydropic degeneration, nuclear pyknosis, and necrosis. The results demonstrated that paracetamol exposure induced moderate hepatocellular injury, characterized by cytoplasmic vacuolization, apoptotic nuclear alterations, and necrotic lesions. Co-treatment with fucoidan at 300 µg/mL was associated with reduced severity across all histopathological parameters, indicating partial hepatoprotective effects. In contrast, the 50 µg/mL fucoidan group exhibited paradoxically severe hydropic degeneration despite the absence of pyknosis and necrosis, suggesting a delayed or altered injury profile. Intermediate outcomes were observed at 100 µg/mL. Overall, fucoidan exhibited dose-dependent but inconsistent hepatoprotective effects. The observed histological variability across concentrations suggests that protection may be influenced by factors such as bioavailability, cellular uptake, or interactions with intracellular stress pathways. These findings highlight the need for further mechanistic investigations before fucoidan can be considered a reliable hepatoprotective agent in aquatic toxicology.
Histopathological Analysis of Liver Damage in Rasbora lateristriata Fish Induced by Extract Butterfly Pea Flower (Clitoria ternatea) Pertiwi, Anggita Endar; Illiyin, Sirayya; Sadewo, Imran; Rahayu, Sekar; Rumansara, Papuani Samparisna; Podhi, Felisitas Moli; Sularsah, Sais; Adelfiani, Adelfiani; Retnoaji, Bambang; Sofyantoro, Fajar; Nuriliani, Ardaning; Wijayanti, Nastiti; Saragih, Hendry T. S. S. G.; Rohmah, Zuliyati; Widiyanto, Slamet; Empra, Desi Eka Putri; Ariesti, Wiwin; Septriani, Nur Indah
RUMPHIUS Vol 8 No 1 (2026): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Butterfly pea flower (Clitoria ternatea) is an Indonesian native plant known for its medicinal properties due to its rich content of anthocyanins, flavonoids, and ternatins. Although widely recognized for its health benefits, the long-term effects of its bioactive compounds require careful evaluation. This study aimed to investigate the potential hepatotoxic effects of butterfly pea flower extract on the liver of Rasbora lateristriata fish. Fish were exposed to 0 (control), 50, 75, and 100 ppm of butterfly pea extract for four days, with four fish allocated per group. Histological markers, including necrosis, vacuolization, pyknosis, and hemorrhage, were assessed in liver tissues. Data were analyzed using the Kruskal-Wallis test and Mann-Whitney U test at a 95% confidence level. Results indicated that butterfly pea flower extract caused significant hepatic damage, with increased severity at higher concentrations. The most pronounced damage was observed at 75 ppm, suggesting an optimal dose for inducing oxidative stress. This study highlights the potential hepatotoxicity of butterfly pea flower extract in aquatic species and underscores the need for further research to evaluate its safety for therapeutic use.
Co-Authors Adelfiani, Adelfiani Aeniah, Siti Allimi, Hayu Swari Andi Gita Maulidyah Indraswari Suhri Anita Restu Puji Raharjeng Apriliyani, Tia Ardaning Nuriliani Arief Maulana Sabilillah Ariel Hananya Ariesti, Wiwin Ascarti Adaninggar Dafa, Maula Haqul Della Blatama Devi Anisa Suci Empra, Desi Eka Putri Fajar Sofyantoro Faulana, Arum Fahmi Febrina Nanda Arfiani Halim, Shuha Ma’muriyah Hayu Swari Alimi Hendry Saragih Hendry T.S.S.G. Saragih Hendry Tri Sakti Saragih Hendry Tri Sakti SG Saragih Hurul Ein Illiyin, Sirayya Jerizqa Ayu Fajar Nita Jessy Octavia Harahap Joko Nugroho Wahyu Karyadi Karlina, Ina Karunia Galih Permadani Khasanah, Luthfia Uswatun Khoiruddin Anshori Khoiruddin Anshori Laili Mufli Zusrina Laili Mufli Zusrina Larasshita Putri Adhitya Sani Lathief Al Umami M. Zainuddin Lubis Maharesi, Chesa Ekani Mayani, Olvita Muhammad Zuchri Zayzda Murtiadi Erlan Supraitno Mustamin, Yusdar Muthia Arrusyda Nastiti Wijayanti Nastiti Wijayanti Nurhidayat, Luthfi Nyan Hayu Widiastuti Oktaviana, Shintia Pertiwi, Anggita Endar Podhi, Felisitas Moli Pradnya Paramita Primahesa, Alfian Prissandi, Anthera Al Firdaus Pusparini, Nur Ainun Oktavia Rahayu, Sekar Rahmadian, Desti Rey, Juniman Ria Vena Septhay Sari Rohmah, Zuliyati Rumansara, Papuani Samparisna Ruth Liananda Citra Dolok Saribu Sadewo, Imran Saeed, Faisal Sandi Francisco Pratama Saragih, Hendry Saragih, Hendry T. S. S. G. Saragih, Hendry Tri Sakti SG Sari, Ria Vinola Septhya Saribu, Ruth Liananda Citra Dolok Septriani, Nur Indah Septriani, Nur Indah Shalihah, Fianicha Sih Kahono Simanungkalit, Eben Ezer Slamet Widiyanto Sri Pujiyati Sularsah, Sais Supraitno, Murtiadi Erlan Susilo Hadi, Susilo Tia Apriliyani Yunita, Neri Zulfadhli Zulfadhli Zuliyati Rohmah Zusrina, Laili Mufli