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All Journal HAYATI Journal of Biosciences Jurnal Ilmu dan Teknologi Kelautan Tropis Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Jurnal Perlindungan Tanaman Indonesia Journal of Tropical Biodiversity and Biotechnology BERITA BIOLOGI Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Jurnal Perikanan Universitas Gadjah Mada JMM (Jurnal Masyarakat Mandiri) Indonesian Journal of Biology Education Biota Journal of Aquaculture and Fish Health JURNAL PERIKANAN TROPIS Borneo Journal of Medical Laboratory Technology Berkala Penelitian Hayati Biological Environment and Pollution Berkala Ilmiah Biologi
Bambang Retnoaji
Fakultas Biologi Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Energy Environmental Science Health Professions Immunology & microbiology Library & Information Science Materials Science & Nanotechnology Medicine & Pharmacology Social Sciences Veterinary Other

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IMPLEMENTASI TEKNOLOGI BUDIDAYA MASSAL DAN PENGOLAHAN PASCA PANEN IKAN WADER PARI Bambang Retnoaji; Joko Nugroho Wahyu Karyadi; Khoiruddin Anshori; Arief Maulana Sabilillah; Lathief Al Umami; Fajar Sofyantoro
JMM (Jurnal Masyarakat Mandiri) Vol 6, No 4 (2022): Agustus
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (458.68 KB) | DOI: 10.31764/jmm.v6i4.9424

Abstract

Abstrak: Desa Wisata Klayar merupakan tujuan wisata alternatif yang ada di Gunungkidul, Yogyakarta. Kelompok sadar wisata (POKDARWIS) Klayar Manunggal berupaya mengembangkan kuliner tradisional ikan wader pari sebagai salah satu daya tarik wisata. Program pengabdian ini bertujuan untuk melakukan pendampingan kepada POKDARWIS Klayar Manunggal agar mampu melakukan pemijahan dan budidaya ikan wader secara mandiri. Rangkaian kegiatan berupa survei lokasi, pembuatan kolam, pemasangan instalasi, pengiriman indukan, pelatihan budidaya, dan monitoring serta evaluasi telah dilaksanakan. Di akhir program, pengurus dan anggota POKDARWIS Klayar Manunggal mampu melakukan pemijahan, budidaya, dan pemrosesan pasca panen ikan wader secara berkelanjutan. Selain itu, POKDARWIS Klayar Manunggal juga menjadi paham dan sadar mengenai pentingnya konservasi ikan wader di habitat alami.Abstract: Klayar Tourism Village is an alternative tourist destination in Gunungkidul, Yogyakarta. The Tourism Awareness Group (POKDARWIS) Klayar Manunggal seeks to develop the traditional culinary of wader fish as a tourist attraction. The current program aimed to provide assistance to POKDARWIS to be able to independently spawn and cultivate wader fish. The programs were carried out through surveys, pond construction, installation, delivery of broodstock, cultivation training, monitoring, and evaluation. In general, the programs have been successfully completed with the cooperation of POKDARWIS Klayar Manunggal. Through this program, POKDARWIS Klayar Manunggal is able to cultivate wader fish in a sustainable manner. In addition, POKDARWIS Klayar Manunggal also understands and is aware of the importance of conserving wader fish in their natural habitat.
PENGARUH KOLOID NANOPERAK TERHADAP PERKEMBANGAN EMBRIO IKAN WADER PARI (Rasbora lateristriata, Bleeker, 1854) Muthia Arrusyda; Bambang Retnoaji
BERITA BIOLOGI Vol 21, No 2 (2022): Berita Biologi
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v21i2.4023

Abstract

Industrialisasi produk dengan bahan nanoperak semakin meningkat. Salah satu metode sintesis nanoperak (AgNPs) yang ramah lingkungan adalah radiosintesis menggunakan sinar gamma. Tantangan lingkungan terkait industrialisasi AgNPs tidak hanya terkait sintesis dan produksi tetapi juga terkait penggunaan produk. Peningkatan penggunaan nanoperak juga berarti peningkatan limbah pemakaian produk dengan nanoperak yang terpapar ke ekosistem perairan. Tujuan penelitian ini adalah untuk mengetahui bagaimana pengaruh koloid nanoperak terstabilisasi alginat terhadap daya tetas telur dan struktur tulang ikan wader pari (Rasbora lateristriata, Bleeker, 1854). Koloid AgNPs disintesis menggunakan sinar gamma pada dosis 5 KGy, 10 KGy, 15 KGy, dan 20 KGy kemudian dikarakterisasi dengan UV-Vis. Telur ikan didedahkan dengan koloid AgNPs selama 24 jam. Embrio diamati pada umur 2 hari menggunakan mikroskop leica. Pewarnaan tulang dilakukan dengan pewarna Alizarin Red dan Alcian Blue pada ikan berusia 5 pekan. Terdapat penurunan daya tetas embrio ikan wader pari pada perlakuan AgNPs yang disitesis pada 5 KGy. Morfologi jantung pada larva megalami edema. Osifikasi pada bagian kranial ikan dengan perlakuan terlihat mengalami keterlambatan jika dibandingkan dengan normal. Terdapat penurunan laju jantung pada ikan perlakuan. Pendedahan AgNPs pada fase embrio ikan wader dapat memengaruhi sistem sirkulasi, struktur tulang dan daya tetas embrio ikan wader pari.
The Effect of Maggot and Silkworm on the Growth and Intestinal Histological Structure of Wader Pari Fish (Rasbora lateristriata Bleeker, 1854) Devi Anisa Suci; Bambang Retnoaji
Jurnal Perikanan Universitas Gadjah Mada Vol 25, No 1 (2023)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.75496

Abstract

Wader pari is one of the fish that is often found in Indonesian waters. One of the problems regarding fish farming is the problem of feed. This study aims to determine the effect of alternative feeding on the growth and histological structure of the Wader pari fish's intestine. The paraffin method was used in constructing intestinal histological preparations by Haematoxylin-Eosin (HE) and Periodic Acid Schiff-Alcian Blue (PAS-AB) staining. Observations were done using the DMRT test on various ANOVA, and the histological structure of the intestine was analyzed descriptively and quantitatively. The treatments used in this study were P1 (commercial feed), P2 (maggot feed), and P3 (mixed feed between maggot and silkworms). The study's results indicated that the higher the protein consumed by the Wader pari fish, the higher the growth. The three treatments that affected the growth of Wader pari sequentially are mixed feed, maggot feed, and commercial feed. The histological structure of the wader pari intestine is composed of tunica mucosa, tunica submucosa, tunica muscularis, and tunica serosa. Maggot and mixed feed can affect the histological structure of the Wader pari intestine compared to commercial feed. This can be seen in the results of the length of the villi and the distribution of the number of goblet cells.
Effect of Chlorpyrifos on Embryo Development of Wader Pari Fish (Rasbora lateristriata Bleeker, 1854) Ascarti Adaninggar; Anita Restu Puji Raharjeng; Larasshita Putri Adhitya Sani; Jessy Octavia Harahap; Della Blatama; Sandi Francisco Pratama; Ardaning Nuriliani; Bambang Retnoaji; Hendry Tri Sakti SG Saragih
Berkala Ilmiah Biologi Vol 13 No 2 (2022): Jurnal Berkala Ilmiah Biologi
Publisher : Fakultas Biologi Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/bib.v13i2.4766

Abstract

Chlorpyrifos is an organophosphate insecticide, acaricide, and nematicide which is used to control leaf pests and soil pest insects. The insecticide chlorpyrifos works by disrupting the insect's nervous system. The use of insecticides on vegetable crops is generally more intensive than on other food crops so the negative impact on the biotic and abiotic environment becomes greater. The purpose of this study was to determine the negative impact of chlorpyrifos exposure with concentrations of 0.01, 0.1, 1 and 10 ppm on the embryonic development of Wader pari (Rasbora lateristriata). The results of observations at 24 and 48 hours showed that there were abnormalities in the development of the Wader pari’s embryo at the concentration of 0,1, 1 and 10 ppm, indicating that chlorpyrifos causes malformations in the form of defects in the development of the embryo of Wader pari fish.
The Potency of Olive Oil, Coconut Oil, and Bleached Palm Oil as Clearing Agent in Histological Preparation Ruth Liananda Citra Dolok Saribu; Laili Mufli Zusrina; Murtiadi Erlan Supraitno; Ardaning Nuriliani; Bambang Retnoaji; Hendry T.S.S.G. Saragih; Zuliyati Rohmah
Berkala Ilmiah Biologi Vol 14 No 2 (2023)
Publisher : Fakultas Biologi Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/bib.v14i2.6528

Abstract

Clearing is an important process in histological preparations. The aim of the clearing process is to remove alcohol from the tissues upon dehydration. The most commonly used clearing agent is xylol, however it is known that there are negative effects from using xylol. This literature study purpose to study the potential of three vegetable oils, i.e., olive oil, coconut oil, and refined palm oil, as clearing agents to replace xylol. This literature study was conducted using search engines such as Semantic Scholar, NCBI, ICAMS, IJPRSE, JOMFP, PubMed, and ResearchGate with the keywords olive oil, coconut oil, palm oil, xylol, clearing agent, and histological preparations. There is no year limitation of references in this literature study. Based on the literatures, it is known that the use of olive oil as a clearing agent produces similar histological preparation qualities to xylol. This can be seen from several criteria that were measured, including the level of tissue translucency and the ability to maintain the quality of the staining. Similar results were shown by purified coconut oil and palm oil, both of which can produce tissues with optimal levels of transparency. The conclusion from this literature study is that refined olive oil, coconut oil, and palm oil can be used as clearing agents for xylol substitutes that are safer for researchers and the environment.
Fixative Solution for Macromolecules in Histological Preparations Hayu Swari Alimi; Ria Vena Septhay Sari; Tia Apriliyani; Ardaning Nuriliani; Bambang Retnoaji; Hendry Saragih; Zuliyati Rohmah
Berkala Ilmiah Biologi Vol 14 No 3 (2023)
Publisher : Fakultas Biologi Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/bib.v14i3.6531

Abstract

The fixation process is subjected to preserve cells, tissues, and their components so that the structure can be maintained as their alive condition and prevent autolysis. This literature review purpose to determine the appropriate use of fixative solutions for several macromolecules, such as proteins, lipids, carbohydrates, and nucleic acids. The literature review data is obtained form the secondary data included books, journals, or articles which discussed about fixative solutions without a limitation on the year of publication. The databases were sourced from Google Scholar, PubMed, NCBI, Science Direct, SpringerLink, Research Gate, and Nature. The keywords for the book search were tissue processing, pathology, and histology. Keywords for searching journals or articles were protein fixation, formaldehyde fixation lipids, fixatives for nucleic acids, Glyo-Fixx fixatives, mercury chloride fixative lipids, formalin-fixed carbohydrates, and ethanol or methanol for fixative lipids. Based on the literature review conducted, it can be concluded that the use of fixative solutions is according to the macromolecules to be observed.
Maturation of Female Yellow Rasbora (Rasbora Lateristriata Bleeker, 1854) Using Oodev at Different Doses in Feed Rey, Juniman; Widiyanto, Slamet; Retnoaji, Bambang
Journal of Tropical Biodiversity and Biotechnology Vol 8, No 3 (2023): December
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.75916

Abstract

The current high demand for Yellow rasbora (Rasbora lateristriata) is not supported by the availability of captured Yellow rasbora in nature. Aquaculture is the most rational way of utilizing biological natural resources. In intensive aquaculture, it is necessary to optimize all processes that occur in aquaculture, including hatchery. However, the common problem that often happens in hatchery activities is spawning which depends on the season. The hormonal manipulation technique is an appropriate way to stimulate gonadal maturation. Oodev is a hormonal combination of pregnant mare serum gonadotropin and anti-dopamine to stimulate gonadal maturation. The purpose of this study was to determine the effectiveness of using the Oodev with different doses in feed to accelerate gonad maturation of female Yellow rasbora. The study was carried out with four treatments and three replications in 21 days with different doses of Oodev, such as; A (Feed without Oodev), B (0.5 mL/kg feed), C (1 mL/kg feed) and D (2 mL/kg feed). The parameters observed in this study were gonad maturity level, histological structure of ovary, gonadosomatic index, fecundity, and diameter of eggs. The results showed that the dose of Oodev at 1.0 mL/kg feed was an effective dose to optimize the gonad maturity of female Yellow rasbora. This is proven by the highest results shown on all parameters, such as; the maturity level in the IV phase, histological structure of the ovary which showed the dominance of the oocyte maturation phase, gonadosomatic index of 14.014%, the fecundity of 721 eggs, and egg diameter of 0.865 mm. In conclusion, using Oodev in feed at a dose of 1.0 ml/kg of feed for 21 days is an effective dose to optimize the maturation of female Yellow rasbora. 
Mangosteen (Garcinia mangostana L.) Peel Decoction Effect on Embryological Development of Wader Pari Fish Rasbora lateristriata (Bleeker, 1854) Khasanah, Luthfia Uswatun; Paramita, Pradnya; Retnoaji, Bambang
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 2 (2024): June
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.80645

Abstract

Mangosteen (Garcinia mangostana L.) is a tropical fruit that has become a sought-after commodity by enthusiasts from various countries, including Indonesia. The active components found in mangosteen peel primarily consist of active xanthone compounds, such as mangostenol, mangostin, mangostino A, mangostino B, tvophylin B, trapezifolixanthone, alpha mangostin, beta mangostin, garcinon B, mangostano, as well as flavonoids epicatechin and gartanin. These compounds exhibit a range of beneficial properties, including anti-inflammatory, antibacterial, antifungal, antihistamine, antidiabetic, anticancer, and more. Consequently, there is significant potential in developing mangosteen peel extract as a valuable ingredient in herbal medicine. However, there is currently no available data on the effects of exposure to mangosteen peel decoction on fish animal models. Therefore, it is essential to investigate the impact of mangosteen peel decoction on wader pari fish (Rasbora lateristriata) embryos. In this study, wader pari embryos were subjected to various concentrations of mangosteen peel decoction (0.5, 1, 5, and 25 µg/mL). The effects on egg hatchability, survival rate (SR), heart rate frequency, and heart morphology of the larvae were meticulously examined using a Leica microscope. The data obtained were subjected to statistical analysis using one-way ANOVA. The findings demonstrated that exposure to mangosteen peel decoction resulted in lower hatching rates and embryonic survival, alongside an increased heart rate frequency. Additionally, the exposed embryos displayed cardiac edema and cardiac bending, particularly at the concentration of 25 µg/mL. In conclusion, the exposure of wader pari fish embryos to mangosteen peel decoction at the concentrations of 25 µg/mL and higher significantly affected the hatching rate, survival rate, and heart rate of R. lateristriata fish larvae. 
Daily Activity and Honey Production Patterns of Tetragonula laeviceps Smith (Hymenoptera: Apidae) During the Wet and Dry Seasons Suhri, Andi Gita Maulidyah Indraswari; Retnoaji, Bambang; Mustamin, Yusdar; Kahono, Sih
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 4 (2024): December
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.84083

Abstract

Honey production by stingless bees is closely related to the foraging activities of worker bees, particularly nectar foraging. The urgency of this study stems from the community's need for adequate understanding and information on honey production which can vary due to various factors, such as forage and season. The purpose of this study was to determine the extent to which foraging activity affects the amount of honey produced by T. laeviceps during the rainy and dry seasons. The focal sampling method was used to observe foraging behaviour and the acetolysis method was used to observe pollen. The abiotic factors were measured through direct observation. Honey production was observed as a result of foraging behaviour and variations in abiotic factors.  In SPSS v27 software, data from foraging behaviour observations were analysed using one-way ANOVA followed by Tukey's test with 95% confidence level and interpreted in tables and figures. Multiple linear regressions and Pearson’s correlations were used to test the relationship between abiotic variables and bee return to hive behaviour. All honey volume data were collected, averaged, and evaluated using bar charts. Based on these results, the amount of honey produced by T. laevicepsin the dry season was significantly higher than that produced during the wet season. This was because the number of bees actively foraging was also higher in the dry season (June-August) which was strongly influenced by temperature and light intensity (p > 0.05). In addition, the number of flowering plants available for harvest during the dry season was higher than that during the rainy season. The results of this study can be used as a reference by beekeepers to determine the appropriate time to harvest honey. Information on the types of forage plants identified in this study can provide information on bee preferences in making choices related to forage plants.
Histopathological evaluation of hepatic tissue of yellow Rasbora (Rasbora lateristriata) exposed to paracetamol Septriani, Nur Indah; Saribu, Ruth Liananda Citra Dolok; Apriliyani, Tia; Karlina, Ina; Pusparini, Nur Ainun Oktavia; Zusrina, Laili Mufli; Sari, Ria Vinola Septhya; Allimi, Hayu Swari; Supraitno, Murtiadi Erlan; Saeed, Faisal; Simanungkalit, Eben Ezer; Paramita, Pradnya; Retnoaji, Bambang; Sofyantoro, Fajar; Wijayanti, Nastiti
Biological Environment and Pollution Vol. 3 No. 1 (2023)
Publisher : Association for Scientific Computing, Electronics, and Engineering (ASCEE)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31763/bioenvipo.v3i1.595

Abstract

Excessive consumption of paracetamol may lead to hepatic necrosis, nephrotoxicity, extra-hepatic lesions, and even fatality in both humans and animals. The objective of this study was to examine the histological changes in the hepatocytes of yellow rasbora (Rasbora lateristriata) exposed to paracetamol. The fish were subjected to varying concentrations of paracetamol over a period of four days. Group I served as the control group without any paracetamol exposure. Groups II, III, and IV were exposed to 2, 3, and 4 mg/L of paracetamol, respectively. A total of six individuals were allocated to each of the four experimental groups. The histological analysis of the hepatic tissue following paracetamol exposure at concentrations of 2, 3, and 4 mg/L revealed hepatic damage characterized by picnosis, necrosis, and vacuolization. In summary, higher concentrations of paracetamol were associated with an increase in the severity of histopathological changes in the hepatic tissues of yellow rasbora.
Co-Authors Aeniah, Siti Allimi, Hayu Swari Andi Gita Maulidyah Indraswari Suhri Anita Restu Puji Raharjeng Apriliyani, Tia Ardaning Nuriliani Arief Maulana Sabilillah Ariel Hananya Ariesti, Wiwin Ascarti Adaninggar Della Blatama Devi Anisa Suci Empra, Desi Eka Putri Fajar Sofyantoro Faulana, Arum Fahmi Febrina Nanda Arfiani Halim, Shuha Ma’muriyah Hayu Swari Alimi Hendry Saragih Hendry T.S.S.G. Saragih Hendry Tri Sakti Saragih Hendry Tri Sakti SG Saragih Hurul Ein Jerizqa Ayu Fajar Nita Jessy Octavia Harahap Joko Nugroho Wahyu Karyadi Karlina, Ina Karunia Galih Permadani Khasanah, Luthfia Uswatun Khoiruddin Anshori Khoiruddin Anshori Laili Mufli Zusrina Laili Mufli Zusrina Larasshita Putri Adhitya Sani Lathief Al Umami M. Zainuddin Lubis Maharesi, Chesa Ekani Muhammad Zuchri Zayzda Murtiadi Erlan Supraitno Mustamin, Yusdar Muthia Arrusyda Nastiti Wijayanti Nastiti Wijayanti Nurhidayat, Luthfi Nyan Hayu Widiastuti Pradnya Paramita Pusparini, Nur Ainun Oktavia Rahmadian, Desti Rey, Juniman Ria Vena Septhay Sari Rohmah, Zuliyati Ruth Liananda Citra Dolok Saribu Saeed, Faisal Sandi Francisco Pratama Saragih, Hendry Saragih, Hendry Tri Sakti SG Sari, Ria Vinola Septhya Saribu, Ruth Liananda Citra Dolok Septriani, Nur Indah Septriani, Nur Indah Shalihah, Fianicha Sih Kahono Simanungkalit, Eben Ezer Slamet Widiyanto Sri Pujiyati Supraitno, Murtiadi Erlan Susilo Hadi, Susilo Tia Apriliyani Yunita, Neri Zulfadhli Zulfadhli Zuliyati Rohmah Zusrina, Laili Mufli