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All Journal HAYATI Journal of Biosciences Jurnal Ilmu dan Teknologi Kelautan Tropis Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Jurnal Perlindungan Tanaman Indonesia Journal of Tropical Biodiversity and Biotechnology BERITA BIOLOGI Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Jurnal Perikanan Universitas Gadjah Mada JMM (Jurnal Masyarakat Mandiri) Indonesian Journal of Biology Education Biota Journal of Aquaculture and Fish Health JURNAL PERIKANAN TROPIS Borneo Journal of Medical Laboratory Technology Berkala Penelitian Hayati Biological Environment and Pollution Berkala Ilmiah Biologi
Bambang Retnoaji
Fakultas Biologi Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Energy Environmental Science Health Professions Immunology & microbiology Library & Information Science Materials Science & Nanotechnology Medicine & Pharmacology Social Sciences Veterinary Other

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Journal : Berkala Ilmiah Biologi

 1 
Effect of Chlorpyrifos on Embryo Development of Wader Pari Fish (Rasbora lateristriata Bleeker, 1854) Ascarti Adaninggar; Anita Restu Puji Raharjeng; Larasshita Putri Adhitya Sani; Jessy Octavia Harahap; Della Blatama; Sandi Francisco Pratama; Ardaning Nuriliani; Bambang Retnoaji; Hendry Tri Sakti SG Saragih
Berkala Ilmiah Biologi Vol 13 No 2 (2022): Jurnal Berkala Ilmiah Biologi
Publisher : Fakultas Biologi Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/bib.v13i2.4766

Abstract

Chlorpyrifos is an organophosphate insecticide, acaricide, and nematicide which is used to control leaf pests and soil pest insects. The insecticide chlorpyrifos works by disrupting the insect's nervous system. The use of insecticides on vegetable crops is generally more intensive than on other food crops so the negative impact on the biotic and abiotic environment becomes greater. The purpose of this study was to determine the negative impact of chlorpyrifos exposure with concentrations of 0.01, 0.1, 1 and 10 ppm on the embryonic development of Wader pari (Rasbora lateristriata). The results of observations at 24 and 48 hours showed that there were abnormalities in the development of the Wader pari’s embryo at the concentration of 0,1, 1 and 10 ppm, indicating that chlorpyrifos causes malformations in the form of defects in the development of the embryo of Wader pari fish.
The Potency of Olive Oil, Coconut Oil, and Bleached Palm Oil as Clearing Agent in Histological Preparation Ruth Liananda Citra Dolok Saribu; Laili Mufli Zusrina; Murtiadi Erlan Supraitno; Ardaning Nuriliani; Bambang Retnoaji; Hendry T.S.S.G. Saragih; Zuliyati Rohmah
Berkala Ilmiah Biologi Vol 14 No 2 (2023)
Publisher : Fakultas Biologi Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/bib.v14i2.6528

Abstract

Clearing is an important process in histological preparations. The aim of the clearing process is to remove alcohol from the tissues upon dehydration. The most commonly used clearing agent is xylol, however it is known that there are negative effects from using xylol. This literature study purpose to study the potential of three vegetable oils, i.e., olive oil, coconut oil, and refined palm oil, as clearing agents to replace xylol. This literature study was conducted using search engines such as Semantic Scholar, NCBI, ICAMS, IJPRSE, JOMFP, PubMed, and ResearchGate with the keywords olive oil, coconut oil, palm oil, xylol, clearing agent, and histological preparations. There is no year limitation of references in this literature study. Based on the literatures, it is known that the use of olive oil as a clearing agent produces similar histological preparation qualities to xylol. This can be seen from several criteria that were measured, including the level of tissue translucency and the ability to maintain the quality of the staining. Similar results were shown by purified coconut oil and palm oil, both of which can produce tissues with optimal levels of transparency. The conclusion from this literature study is that refined olive oil, coconut oil, and palm oil can be used as clearing agents for xylol substitutes that are safer for researchers and the environment.
Fixative Solution for Macromolecules in Histological Preparations Hayu Swari Alimi; Ria Vena Septhay Sari; Tia Apriliyani; Ardaning Nuriliani; Bambang Retnoaji; Hendry Saragih; Zuliyati Rohmah
Berkala Ilmiah Biologi Vol 14 No 3 (2023)
Publisher : Fakultas Biologi Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/bib.v14i3.6531

Abstract

The fixation process is subjected to preserve cells, tissues, and their components so that the structure can be maintained as their alive condition and prevent autolysis. This literature review purpose to determine the appropriate use of fixative solutions for several macromolecules, such as proteins, lipids, carbohydrates, and nucleic acids. The literature review data is obtained form the secondary data included books, journals, or articles which discussed about fixative solutions without a limitation on the year of publication. The databases were sourced from Google Scholar, PubMed, NCBI, Science Direct, SpringerLink, Research Gate, and Nature. The keywords for the book search were tissue processing, pathology, and histology. Keywords for searching journals or articles were protein fixation, formaldehyde fixation lipids, fixatives for nucleic acids, Glyo-Fixx fixatives, mercury chloride fixative lipids, formalin-fixed carbohydrates, and ethanol or methanol for fixative lipids. Based on the literature review conducted, it can be concluded that the use of fixative solutions is according to the macromolecules to be observed.
Co-Authors Aeniah, Siti Allimi, Hayu Swari Andi Gita Maulidyah Indraswari Suhri Anita Restu Puji Raharjeng Apriliyani, Tia Ardaning Nuriliani Arief Maulana Sabilillah Ariel Hananya Ariesti, Wiwin Ascarti Adaninggar Della Blatama Devi Anisa Suci Empra, Desi Eka Putri Fajar Sofyantoro Faulana, Arum Fahmi Febrina Nanda Arfiani Halim, Shuha Ma’muriyah Hayu Swari Alimi Hendry Saragih Hendry T.S.S.G. Saragih Hendry Tri Sakti Saragih Hendry Tri Sakti SG Saragih Hurul Ein Jerizqa Ayu Fajar Nita Jessy Octavia Harahap Joko Nugroho Wahyu Karyadi Karlina, Ina Karunia Galih Permadani Khasanah, Luthfia Uswatun Khoiruddin Anshori Khoiruddin Anshori Laili Mufli Zusrina Laili Mufli Zusrina Larasshita Putri Adhitya Sani Lathief Al Umami M. Zainuddin Lubis Maharesi, Chesa Ekani Muhammad Zuchri Zayzda Murtiadi Erlan Supraitno Mustamin, Yusdar Muthia Arrusyda Nastiti Wijayanti Nastiti Wijayanti Nurhidayat, Luthfi Nyan Hayu Widiastuti Pradnya Paramita Pusparini, Nur Ainun Oktavia Rahmadian, Desti Rey, Juniman Ria Vena Septhay Sari Rohmah, Zuliyati Ruth Liananda Citra Dolok Saribu Saeed, Faisal Sandi Francisco Pratama Saragih, Hendry Saragih, Hendry Tri Sakti SG Sari, Ria Vinola Septhya Saribu, Ruth Liananda Citra Dolok Septriani, Nur Indah Septriani, Nur Indah Shalihah, Fianicha Sih Kahono Simanungkalit, Eben Ezer Slamet Widiyanto Sri Pujiyati Supraitno, Murtiadi Erlan Susilo Hadi, Susilo Tia Apriliyani Yunita, Neri Zulfadhli Zulfadhli Zuliyati Rohmah Zusrina, Laili Mufli