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SKRINING KOMPONEN BIOAKTIF EKSTRAK BAMBU LAUT (Isis hippuris) DARI PERAIRAN SULAWESI TENGAH Muliadin Muliadin; Didit Kustantio Dewanto; Deddy Wahyudi; Wendy Alexander Tanod; Putut Har Riyadi; Firman Farid Muhsoni
Jurnal Kelautan dan Perikanan Terapan (JKPT) Vol 5, No 1 (2022): JKPT Juni 2022
Publisher : Politeknik Ahli Usaha Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jkpt.v5i1.10596

Abstract

Bambu laut (Isis hippuris) merupakan salah satu organisme laut penyusun ekosistem terumbu karang yang dilaporkan berpotensi sebagai sumber bahan bioaktif. Bambu laut mengandung bioaktif polioksigenasi steroid, hidrokarbon, fenol dan asam lemak. Penelitian ini bertujuan mengonfirmasi komponen bioaktif dari ekstrak bambu laut. Sampel bambu laut dikoleksi dari perairan Kabupaten Donggala, Sulawesi Tengah, dengan kondisi cuaca cerah. Pada sampel bambu laut dilakukan proses ekstraksi (maserasi MeOH : DCM), skrining komponen bioaktif (metode Harborne), dan determinsi kandungan total alkaloid (ekuivalen kafein - CE), fenol (ekuivalen asam galat - GAE), flavonoid (ekuivalen kuersetin - QE), dan steroid (ekuivalen kolesterol - ChE). Hasil skrining komponen bioaktif menunjukkan ekstrak bambu laut (I. hippuris) mengandung sejumlah komponen alkaloid, fenol, steroid dan flavonoid. Ekstrak bambu laut mengandung alkaloid 11,61 ± 0,24 mg CE.g-1 ekstrak kering; fenol sebesar 18,92 ± 0,24 mg GAE.g-1 ekstrak kering; flavonoid sebesar 21,24 ± 0,28 mg QE.g-1 ekstrak kering; dan steroid sebesar 36.94 ± 1.39 mg ChE.g-1 ekstrak kering. Penelitian ini mengonfirmasi keberadaan komponen bioaktif ekstrak bambu laut (I. hippuris) yang dikoleksi dari perairan Sulawesi Tengah. Hasil skrining komponen bioaktif menunjukkan kehadiran komponen alkaloid, fenol, steroid dan flavonoid. Oleh karena itu, perlu diidentifikasi lebih lanjut senyawa bioaktif (diduga turunan steroid, alkaloid dan fenolik) yang terkandung dari ekstrak bambu laut.
GAMBARAN PERMASALAHAN TIDAK TERCAPAINYA PERILAKU HIDUP BERSIH DAN SEHAT PADA MASYARAKAT PESISIR KAMPUNG LIKUANG KEPULAUAN SANGIHE Dhito Dwi Pramardika; Meityn Disye Kasaluhe; Yana Sambeka; Wendy Alexander Tanod; Astri Juwita Mahihody
Jurnal Ilmiah Sesebanua Vol 6 No 2 (2022): Jurnal Ilmiah Sesebanua
Publisher : Pusat Penelitian dan Pengabdian Pada Masyarakat, POLITEKNIK NEGERI NUSA UTARA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54484/jis.v6i2.530

Abstract

Program perilaku hidup bersih dan sehat di Indonesia sudah berlangsung 26 tahun namun hanya 39,1 persen keluarga di Indonesia yang menerapkan PHBS. Kabupaten Kepulauan Sangihe yang berada paling utara Indonesia dan mayoritas masyarakatnya bermata pencaharian sebagai nelayan hidup di wilayah pesisir pantai. Sekitar 45,4 persen penduduknya berpendidikan sekolah dasar dan capaian keluarga ber-PHBS tahun 2021 sebesar 18,3 persen. Berdasarkan hal tersebut, penelitian ini bertujuan untuk mengetahui gambaran permasalahan tidak tercapainya perilaku hidup bersih dan sehat pada masyarakat pesisir. Jenis penelitian yang digunakan penelitian deskriptif dengan metode survei yang dilaksanakan di Kampung Likuang Kecamatan Tabukan Utara, Kabupaten Kepulauan Sangihe dengan sampel 124 responden. Pengambilan sampel menggunakan teknik purpossive sampling. Instrumen berupa kuesioner yang telah diuji validitas dan realibilitasnya. Analisis data berupa analisis deskriptik dengan distribusi frekuensi.Hasil penelitian yaitu pengetahuan responden mengenai PHBS sebesar 57,3 persen tidak baik, sikap responden mengenai PHBS sebesar 58,1 persen tidak baik dan tindakan responden mengenai PHBS sebesar 51,6 persen baik. Dapat disimpulkan bahwa rendahnya pengetahuan PHBS pada indikator ASI Ekslusif, Air bersih, mencuci tangan, dan kebiasaan merokok. Rendahnya sikap PHBS pada indikator ASI ekslusif, makan sayur dan buah, aktivitas fisik, dan cuci tangan. Kemudian rendahnya tindakan PHBS pada indikator ASI ekslusif, cuci tangan, jamban sehat, dan pemantauan jentik nyamuk. Oleh karena itu, Kampung Likuang perlu dilakukan pembinaan PHBS. Hygiene and Healthy Lifestyle Behavior (HHLB) coaching programs in Indonesia have been going on for 26 years, but only 39.1 percent of families in Indonesia have implemented HHLB (PHBS). The Sangihe Island Regency is located in the northernmost part of Indonesia, and most of it is people who live as fishermen in coastal areas 45.4 percent of the population has an elementary school education, and the achievement of families with HHLB in 2021 is 18.3 percent. Based on it this study aims to describe the problem of unachieved hygiene and healthy living behavior in coastal communities. This research used descriptive research with a survey method in Likuang Village, North Tabukan District, Sangihe Island Regency, with a sample of 124 respondents. Sampling using a purposive sampling technique. The instrument is a questionnaire that has been tested for validity and reliability. Data analysis is in the form of descriptive analysis with frequency distribution. The study's results showed that the respondent's knowledge about HHLB was 57.3 percent not good, the respondent's attitude about HHLB PHBS was 58.1 percent not good, and the respondent's actions regarding HHLB were 51.6 percent good. It can be concluded that the common knowledge of HHLB is on the indicators of exclusive breastfeeding, hygiene water, washing hands, and smoking habit. Low HHLB attitude on indicators of exclusive breastfeeding, eating vegetables and fruit, physical activity, and washing hands. Then the low HHLB measures indicators of exclusive breastfeeding, hand washing, healthy latrine, and monitoring of mosquito larvae. Therefore, Likuang Village needs to be fostered by HHLB.
Pengenalan Kegiatan Peregangan Tubuh Sebagai Aktivitas Fisik Dalam Menunjang Pembelajaran Efektif Siswa Sekolah Dasar Dhito Dwi Pramardika; Meityn Disye Kasaluhe; Wendy Alexander Tanod; Ferdinand Gansalangi; Yana Sambeka; Eko Cahyono; Destu Ayu Hapsari
Jurnal Empathy Pengabdian Kepada Masyarakat Jurnal Empathy Pengabdian Kepada Masyarakat Volume 3 Nomor 1 Tahun 2022
Publisher : Poltekkes Kemenkes Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37341/jurnalempathy.v0i0.119

Abstract

Background: Teachers as educators have challenges in their student's learning during the transition from a pandemic to a COVID-19 endemic. Therefore, strategies and innovations are needed to improve effective education, especially in increasing concentration and body fitness. This is also a problem faced at SDN Inpres Enemawira, Tabukan Utara District, Sangihe Islands Regency. Based on this, the team aims to introduce body stretching training as a physical activity to support learning more effectively. Methods: This service uses an educational approach with 11 stages of health planning. The data collection technique is an observation method with a success indicator of 80% of students' ability in body stretching developing as expected. This program targets students in grades 4, 5, and 6, held on July 23, 2022, by the service team of the Nusa Utara State Polytechnic. Results: The results of this activity were as many as 18 students participated in the activity. Then the evaluation results showed that the completeness of 88.9% of participants had developed in following and repeating body stretching movements for three repetitions. Conclusion: The introduction of body stretching activities can support learning more effectively than before being introduced to body-trying activities. It can be seen by increasing students' concentration power and physical fitness.
Penerapan Tungku Pengasapan Tebiyama Pada Pembuatan Ikan Kayu Frets Jonas Rieuwpassa; Aprelia M. Tomasoa; Wendy A. Tanod; Edward Irsan L. Ferre; Marnens Ch. Karaeng
Jurnal FishtecH Vol 11, No 2 (2022)
Publisher : Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36706/fishtech.v11i2.14271

Abstract

The tebiyama is an alternative for producing wooden fish in the islands such as ’Nusa Utara’ which is abundant in the production of small pelagic fish, especially Decapterus sp. and Euthynnus affinis. The purpose of this study was to see the chemical quality of wooden fish produced using by tebiyama. The stages consist of the preparation stages including weighing, cutting, washing, boiling, removing the bones, drying 1 and drying 2. Furthermore, the fish is smoked using a tebiyama for 30 hours at 60-65oC, every 10 hours the fish is rested for 24 hours. The wooden fish was calculated yield and proximate. The yield results were obtained for Decapterus sp. by 35,9% and Euthynnus affinis by 42,9%. The proximate (moisture, protein, fat and ash) showed that the wooden fish from Decapterus sp. had a moisture content of 16.16%, a fat content of 7.81%, a protein content of 70.02% and an ash content of 2.31% while the wooden fish from Euthynnus affinis had a moisture content of 16.06. %, fat content 10.12%, protein content 70.72% and ash content 2.5%. The moisture content of wooden fish produced with the tebiyama meets SNI 2691.1-2009.
Pemberdayaan Masyarakat Melalui Kegiatan Pembibitan dan Penanaman Mangrove di Pantai Salurang, Kepulauan Sangihe Frets Jonas Rieuwpassa; Indra Wibowo; Wendy A. Tanod; Jaka F.P. Palawe; Eko Cahyono; Stevy I. M. Wodi; Novalina M. Ansar; Obyn I. Pumpente; Aprelia M. Tomasoa; Usy N. Manurung; Eunike I. Kumaseh; Fitria F. Lungary; Herjumes Aatjin; Christian A. Manansang; Steward I. Makawekes; Anggraini Barlian; Walter Balansa
Wikrama Parahita : Jurnal Pengabdian Masyarakat Vol. 7 No. 1 (2023): Mei 2023
Publisher : Universitas Serang Raya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30656/jpmwp.v7i1.5336

Abstract

Mangrove memainkan peran sangat krusial bukan saja sebagai pelindung pesisir pantai tetapi juga sebagai tempat perkembangbiakan dan hunian beragam organisme dan sebagai sumber berbagai bahan bioaktif berpotensi medis. Kerusakan vegetasi mangrove di Kampung Salurang Kabupaten Sangihe yang merupakan daerah hilir akibat aktivitas tambang di daerah hulu menyebabkan penurunan drastis hasil tangkapan ikan di kampung Salurang dan musnahnya berbagai sumber bahan bioaktif potential. Kegiatan pengabdian masyarakat ini bertujuan memulihkan vegetasi mangrove yang telah rusak di kampung Salurang. Tahapan kegiatan pengabdian meliputi (1) survei dan konsultasi dengan pemerintah kampung untuk menentukan lokasi penanaman dan tanggal pemberian materi penyuluhan, (2) pendampingan untuk pembibitan mangrove oleh tim kepada masyarakat, (3) pelaksanaan kegiatan mencakup pemberian materi kepada masyarakat termasuk pemuda dan siswa sekolah menengah pertama dan penanaman mangrove bersama masyarakat. Hingga saat ini, sekitar 76% dari 95% mangrove yang ditanam tumbuh dengan baik dan pertumbuhannya masih terus dipantau. Kegiatan ini memiliki implikasi penting bukan saja untuk lingkungan dan kehidupan masyarakat pesisir tetapi juga untuk melindungi organisme simbion mangrove maupun mangrove itu sendiri sebagai sumber berbagai bahan bioaktif potensial.
Monitoring the endemic ornamental fish Pterapogon kauderni in Bokan Kepulauan, Banggai marine protected area, Indonesia Samliok Ndobe; Kris Handoko; Deddy Wahyudi; Moh. Yasir; Yulina Irawati; Wendy Alexander Tanod; Abigail Mary Moore
Depik Vol 9, No 1 (2020): April 2020
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (880.877 KB) | DOI: 10.13170/depik.9.1.15363

Abstract

Abstract. The Banggai cardinalfish Pterapogon kauderni is a species of national and international conservation concern. Established in November 2019, the Banggai marine protected area (MPA) in Central Sulawesi, Indonesia covers most of the endemic range of this ornamental fish. The third repeat survey (T2 monitoring) under the National Action Plan for Banggai Cardinalfish Conservation (NAP-BCFC) was carried out in October 2019 at eight sites in the Bokan Kepulauan region within the MPA. The T2 monitoring used the standard NAP-BCFC belt transect method. Data were collected on P. kauderni abundance (by size class: recruits, juveniles, adults) and microhabitat (sea urchins, sea anemones, hard corals, and others). Data were evaluated with respect to the T0 (2017) survey and T1 (2018) monitoring at the same sites, as well as previous surveys in 2004 (2 sites) and 2012 (4 sites). The data show wide between-site variation in P. kauderni and microhabitat parameters, with one subpopulation at very high risk of extirpation. Trends included declines over time in P. kauderni, sea urchin and sea anemone abundance, with an increase since 2017 in hard coral microhabitat use by adult P. kauderni. We recommend evaluation of other P. kauderni populations in Bokan Kepulauan and specific site or zone-based actions. However, we conclude that the most urgent priority for P. kauderni conservation in Bokan Kepulauan is protection of key microhabitat through a moratorium on sea urchin and sea anemone collection in P. kauderni habitat.  Keywords: Banggai cardinalfish, endangered species, marine conservation, microhabitat, monitoring, ornamental fishery, CITES
Monitoring the endemic ornamental fish Pterapogon kauderni in Bokan Kepulauan, Banggai marine protected area, Indonesia Samliok Ndobe; Kris Handoko; Deddy Wahyudi; Moh. Yasir; Yulina Irawati; Wendy Alexander Tanod; Abigail Mary Moore
Depik Vol 9, No 1 (2020): April 2020
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.9.1.15363

Abstract

Abstract. The Banggai cardinalfish Pterapogon kauderni is a species of national and international conservation concern. Established in November 2019, the Banggai marine protected area (MPA) in Central Sulawesi, Indonesia covers most of the endemic range of this ornamental fish. The third repeat survey (T2 monitoring) under the National Action Plan for Banggai Cardinalfish Conservation (NAP-BCFC) was carried out in October 2019 at eight sites in the Bokan Kepulauan region within the MPA. The T2 monitoring used the standard NAP-BCFC belt transect method. Data were collected on P. kauderni abundance (by size class: recruits, juveniles, adults) and microhabitat (sea urchins, sea anemones, hard corals, and others). Data were evaluated with respect to the T0 (2017) survey and T1 (2018) monitoring at the same sites, as well as previous surveys in 2004 (2 sites) and 2012 (4 sites). The data show wide between-site variation in P. kauderni and microhabitat parameters, with one subpopulation at very high risk of extirpation. Trends included declines over time in P. kauderni, sea urchin and sea anemone abundance, with an increase since 2017 in hard coral microhabitat use by adult P. kauderni. We recommend evaluation of other P. kauderni populations in Bokan Kepulauan and specific site or zone-based actions. However, we conclude that the most urgent priority for P. kauderni conservation in Bokan Kepulauan is protection of key microhabitat through a moratorium on sea urchin and sea anemone collection in P. kauderni habitat.  Keywords: Banggai cardinalfish, endangered species, marine conservation, microhabitat, monitoring, ornamental fishery, CITES
Growth Characteristics of Vibrio parahaemolyticus Isolated from Lobster (Panulirus sp.) Under Different Temperatures, pH, and NaCl Concentrations Novalina Maya Sari Ansar; Frans Gruber Ijong; Wendy Alexander Tanod; Eko Cahyono; Obyn Imhart Pumpente; Jaka Frianto Putra Palawe
Bioma : Berkala Ilmiah Biologi Vol. 25, No 1, Tahun 2023
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/bioma.25.1.74-87

Abstract

Vibrio parahaemolyticus is a bacterium found in estuaries and marine and is a pathogenic bacterium that harms human health. Vibrio parahaemolyticus can contaminate fishery products and potentially contaminate lobster products in North Sulawesi. This study was conducted to determine the presence of V. parahaemolyticus in lobsters collected in two shelters in North Sulawesi. This study also serves as a monitoring function and a means of information on the presence of V. parahaemolyticus. The samples used were lobsters (Panulirus sp.) taken from Malalayang and Tuminting shelters in North Sulawesi. Observations in this study include total bacteria, total Vibrio, and identified V. parahaemolyticus. Isolates of V. parahaemolyticus were characterized by their growth at different temperatures (5, 37, 43 oC); pH (5 - 9); and concentrations of NaCl (0, 1, 3, 5%). The results show total plates for lobster meat from Malalayang 4.3×104 ‒ 1.0×105 CFU/g, while from Tuminting shelter 1.4×104 ‒ 3.9×104 CFU/g. The total plate on lobster gills from Malalayang is 6.2×104 ‒ 1.2×105 CFU/g, while from Tuminting shelter 2.0×104 ‒ 6.7×104 CFU/g. Total Vibrio in lobster meat from Malalayang 6.2×103 ‒ 1.4×104 CFU/g, while from Tuminting shelter 5.2×103 ‒ 7.9×103 CFU/g. Total Vibrio in lobster gills from Malalayang 8.5×103 ‒ 4.6×104 CFU/g, while from Tuminting shelter 7.8×103 ‒ 9.5×103 CFU/g. The Gram staining analysis obtained 96 isolate strains with Gram-negative rods, and 42 strains showed characteristics as V. parahaemolyticus based on the biochemical assay. Vibrio parahaemolyticus can grow optimally at 37°C, pH 7‒8, and NaCl concentration 3‒5%.
Efficacy and Physiological Responses of Payangka Fish (Ophieleotris aporos) to Anesthetization with Clove Oil Livana Dethris Rawung; Wendy Alexander Tanod; Nonny Manampiring
Jurnal Penelitian Pendidikan IPA Vol 9 No SpecialIssue (2023): UNRAM journals and research based on science education, science applic
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9iSpecialIssue.4726

Abstract

This study aims to determine the effect of clove oil concentration and time of immersion in clove oil on the anesthesia, recovery time, and physiological responses in the payangka fish (Ophieleotris aporos). The experimental design was completely randomized with a 6 x 3 factorial arrangement. The first factor was a concentration of clove oil consisting of six levels i.e., 0.02; 0.03; 0.04; 0.05; 0.1, and 0.2 ml/L of water. The second factor was a time of immersion consisting of three levels i.e., 1; 3; and 6.5 hours. Results showed that the concentration of clove oil significantly (p<0.05) affected the anesthesia time and the recovery time after immersing. The length of immersing and concentration of clove oil also significantly (p<0.05) affected the concentration of glucose, but not significantly (p>0.05) affected the concentration of cholesterol. It showed that clove oil can be used as anesthetization for payangka fish in the appropriate dose. The optimal concentration of clove oil that can be used as anesthesia for the transportation of live payangka fish is 0.02 ml/L of water. Meanwhile, for handling activities on payangka fish, a clove oil concentration of 0.03 ml/L of water is recommended
Monitoring the endemic ornamental fish Pterapogon kauderni in Bokan Kepulauan, Banggai marine protected area, Indonesia Samliok Ndobe; Kris Handoko; Deddy Wahyudi; Moh. Yasir; Yulina Irawati; Wendy Alexander Tanod; Abigail Mary Moore
Depik Vol 9, No 1 (2020): April 2020
Publisher : Faculty of Marine and Fisheries, Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13170/depik.9.1.15363

Abstract

Abstract. The Banggai cardinalfish Pterapogon kauderni is a species of national and international conservation concern. Established in November 2019, the Banggai marine protected area (MPA) in Central Sulawesi, Indonesia covers most of the endemic range of this ornamental fish. The third repeat survey (T2 monitoring) under the National Action Plan for Banggai Cardinalfish Conservation (NAP-BCFC) was carried out in October 2019 at eight sites in the Bokan Kepulauan region within the MPA. The T2 monitoring used the standard NAP-BCFC belt transect method. Data were collected on P. kauderni abundance (by size class: recruits, juveniles, adults) and microhabitat (sea urchins, sea anemones, hard corals, and others). Data were evaluated with respect to the T0 (2017) survey and T1 (2018) monitoring at the same sites, as well as previous surveys in 2004 (2 sites) and 2012 (4 sites). The data show wide between-site variation in P. kauderni and microhabitat parameters, with one subpopulation at very high risk of extirpation. Trends included declines over time in P. kauderni, sea urchin and sea anemone abundance, with an increase since 2017 in hard coral microhabitat use by adult P. kauderni. We recommend evaluation of other P. kauderni populations in Bokan Kepulauan and specific site or zone-based actions. However, we conclude that the most urgent priority for P. kauderni conservation in Bokan Kepulauan is protection of key microhabitat through a moratorium on sea urchin and sea anemone collection in P. kauderni habitat.  Keywords: Banggai cardinalfish, endangered species, marine conservation, microhabitat, monitoring, ornamental fishery, CITES
Co-Authors AA Sudharmawan, AA Abigail Mary Moore Adel, Yeldi S Agus Turambi Alismi M Salanggon Alismi M Salanggon Amelia, Resky Anggraini Barlian Anita Treisya Aristawati Anita Treisya Aristawati Aprelia M. Tomasoa Aprelia M. Tomasoa Aprelia Martina Tomasoa, Aprelia Martina Asriani Hasanuddin Astri Juwita Mahihody Azhari, Deidy Balansa, Walter Biahimo, Syahputra Bilqis, Dewi Shofya Cahyono, Eko Christian A. Manansang Deddy Wahyudi Deddy Wahyudi Deddy Wahyudi Deddy Wahyudi Deddy Wahyudi Destu Ayu Hapsari Destu Ayu Hasari Dewanto, Didit Kustantio Dewanto, Didit Kustantio Dewanto, Didit Kustantio Didit Kustantio Dewanto Didit Kustantio Dewanto Didit Kustantio Dewanto Didit Kustantio Dewanto Didit Kustantio Dewanto Didit Kustantio Dewanto Didit Kustantio Dewanto Edward Irsan L. Ferre EKO CAHYONO Eko Cahyono Eko Cahyono Eko Cahyono, Eko Ely John Karimela Eunike Irene Kumaseh Finarti Finarti Finarti Finarti Finarti, Finarti Firman Farid Muhsoni Fitria F. Lungary Frans Gruber Ijong Frets Jonas Rieuwpassa Frets Jonas Rieuwpassa Gansalangi Ferdinand hanifah hanifah Herjumes Aatjin Indra Wibowo Jaka F.P. Palawe Jaka Frianto Putra Palawe Janis, Ronaldo Ferdinan Jefri Antonius Mandeno Jimi Palawe Kasaluhe, Meityn Kendek, Tasya Jenifel Kris Handoko Kundiman, Gita Artika Putri Lawendatu, Angryani Enjel Lawendatu, Angryani Enjela Livana Dethris Rawung Maalua, Sheyla Magie Kapojos Mahihody, Astri Mahihody, Astri Juwita Makisake, Nadia Arlina Mandalika, Ervina Dian Mandeno, Jefri Anthonius Mandiangan, Meyrijean Marnens Ch. Karaeng Masteria Yunovilsa putra Meityn Disye Kasaluhe Moh. Yasir Mohamad Akbar Muliadin Muliadin Muliadin Muliadin Muliadin Muliadin Muliadin Muliadin Muliadin, Muliadin Musdalifah Musdalifah Nonny Manampiring Novalina M. Ansar Novalina Maya Sari Ansar Novelia Tatinting Obyn I. Pumpente Obyn Imhart Pumpente Pramardika, Dhito Dwi Putra, Masteria Yunovilsa Putut Har Riyadi Putut Har Riyadi Remy E. P Mangindaan Renol Renol Renol, Renol Revolson Mege Roni Hermawan Roni Hermawan Roni Hermawan Roni Hermawan Roni Hermawan Roni Hermawan, Roni Rorong, Miranda Salanggon, Alismi M Samliok Ndobe Sari Aswani Septania Takasiaheng Siegfried Berhimpon Siti Aisiah Somba, Vira Valentine Stevy I. M. Wodi Stevy Imelda Murniati Wodi Steward I. Makawekes Tampany, Ighen Tampi, Regina Romalya Meilan Tengkue, Fiorenza Puspa Indah Tengkue, Florenza Puspa Indah Tinungki, Maria Notosari Trivonia Kerol Talete Usy N. Manurung Walter Balansa Wodi, Stevy Imelda Murniati Yana Sambeka Yana Sambeka Yeldi S Adel Yeldi S Adel Yulina Irawati Zakinah A Alhaddad