Claim Missing Document
Check
Articles

Found 2 Documents
Search
Journal : Journal Of Carbazon

Testing Antioxidant Activity of Pineapple (Ananas Comossus Merr.) Rinds Using UV-Vis Spectrophotometry Method Syahputra, Andi; Halimatussakdiah, Halimatussakdiah; Amna, Ulil
Journal of Carbazon Vol 2, No 1 (2024): June 2024
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jocarbazon.v2i1.37830

Abstract

Pineapple (Ananas comossus Merr.) is one of the plants that is widespread in tropical and subtropical areas. This plant content vitamin C and antioxidant activity. This study was conducted to identify the class of secondary metabolite compounds and determine the antioxidant activity of pineapple fruit peel. Antioxidant activity testing on pineapple fruit peel was conducted using UV-Vis spectophotometric method with wavelength of 514 nm. The identification of secondary metabolite compounds showed that the methanol extract of pineapple fruit peel contains alkaloid, flavonoid, phenol, tannin and saponin compounds. Antioxidant activity of methanol extract of pineapple fruit peel carried out by DPPH (1,1-diphenyl-2-picryldhydrazyl) method shows IC50 value of 92.87 ppm with strong category, while vitamin C as a comparison has IC50 value of 9.31 ppm with very strong category. This is due to the pineapple fruit peel containing high levels of vitamin C. In addition, pineapple fruit peel contains secondary metabolites, especially flavonoids, phenols and tannins which are a class of polyphenolic compounds.
Determination Of Total Flavonoids Extract of White (Magnolia Alba (Dc.) Figlar) Using Spectrophotometry UV-Vis Method Mastura, Mastura; Amna, Ulil; Niaci, Sarah; Pebiola, Tia
Journal of Carbazon Vol 2, No 1 (2024): June 2024
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jocarbazon.v2i1.37890

Abstract

Flavonoids are a group of secondary metabolites produced by plants that have the potential to influence pharmacological activity. This study aims to determine the total flavonoid content of the ethanol crude extract of white cempaka flowers (Magnollia alba(DC.) Figlar) using the UV-Vis spectrophotometry method. This study used colorimetric and spectrophotometric methods using quercetin as a comparison. The qualitative test research results proved that cempaka putih (Magnolia alba(DC.) Figlar) flowers contain flavonoid coumpound. Then a maximum wavelength of 432 nm is obtained with an absorbance of 0.1236, the absorbance calculation for each quercetin concentration and the sample is calculated using a maximum wavelength of 432 nm. Based on the linear regression equation obtained from the standard quercetin curve, the total flavonoid content of the crude ethanol extract of cempaka putih flowers with 3 different concentration variations, at a concentration of 100 ppm, the results obtained for the total flavonoid content were 276,83 17,7 mgQE/g, 500 ppm. total flavonoid levels of 105,189 8,16 mgQE/g, 1000 ppm obtained total flavonoid levels of 79,115 8,33 mgQE/g.