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All Journal ODONTO Dental Journal Majalah Obat Tradisional Dentino: Jurnal Kedokteran Gigi Dental Journal (Majalah Kedokteran Gigi) Dentin Jurnal Radiologi Dentomaksilofasial Indonesia (JRDI) Odonto dental journal Jurnal Bioteknologi & Biosains Indonesia (JBBI)

Aspriyanto, Didit

Department Of Dentomaxillofacial Radiology, Faculty Of Dentistry, Universitas Lambung Mangkurat, Banjarmasin, Indonesia, 70236

Author-ID : 681942

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Dentistry Energy Environmental Science Health Professions Medicine & Pharmacology Physics Public Health

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TOTAL COLONY CHARACTERIZATION OF AEROBIC BACTERIA OF THE ORAL CAVITY ON PEATLANDS WATER AND PDAM WATER Bayu Yordha Senggara; Deby Kania Tri Putri; Didit Aspriyanto
Dentino : Jurnal Kedokteran Gigi Vol 3, No 1 (2018)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v3i1.4619

Background: South Kalimantan peatland water is approximately 8000 km2. Barito Kuala has 40,858 hectares or 90% of the area classified as peatland water. Society uses peatlands water with acidic pH(3.5-4.5) for everyday purposes including rinsing and brushing teeth. The acidic nature of peatland water can affect the growth of bacterial colonies in the oral cavity so that the acidic conditions of the oral cavity increases and the effect on the occurrence of dental caries. In addition PDAM water is water from river water that is processed through several stages to become clean water after filtration and disinfection stages to eliminate microorganisms. Objective: this study aims to determine the characterization of the number of colonies of aerobic bacteria of the oral cavity on peatland water and PDAM water. Methods: This study used quasi experimental method with post test only control group design. The study sample consisted of 30 respondents. Research materials were 4ml of a result of the salivary gargle on peatland water and PDAM water then the number of aerobic bacteria colonies were calculated by the method of TPC (Total Plate Count) and characterization of aerobic bacteria was done by using catalase test, test MSA and coagulase test. Results: This study showed that there were 1653 CFU/ml of aerobic aerobic colony, whereas in water of PDAM there were 1264 CFU/ml of aerobic colonies. In the characterization tests of aerobic bacteria colonies showed that there were Staphylococcus aureus and Staphylococcus epidermidis bacteria on gargle water of peatland or PDAM water. Independent t-test results showed significant differences in aerobic bacterial colonies in peatland water and PDAM water (p = 0.001)(p = 0.002)(p <0.05). Conclusion: Based on this study it can be concluded that the number of Staphylococcus aureus and Staphylococcus epidermidis found in the oral cavity of children saliva who gargled with peatland water are higher than the number of Stapylococcus aureus and Staphylococcus epidermidis found in the saliva of children who gargle with PDAM water.

THE EFFECTS OF PANORAMIC RADIOGRAPHY ON GINGIVAL CREVICULAR FLUID VOLUME IN GINGIVITIS Rini Rahmiyati; Didit Aspriyanto; Beta Widya Oktiani
Dentino : Jurnal Kedokteran Gigi Vol 6, No 2 (2021)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v6i2.11995

Background: Gingival crevicular fluid (GCF) is a biological fluid derived from the gingival sulcus and can be elevated in the inflammatory state of periodontal tissue, such as gingivitis. In previous studies, the number of GCF could also increase after panoramic radiographic exposure. Increase in GCF due to panoramic radiography is a sign of cell damage. Objective: To analyze the effects of panoramic radiography on the volume of GCF in wistar rats with gingivitis. Method: This type of research was true experimental with post test only and control group design. The sampling technique used was simple random sampling. A total of 25 wistar rats were classified into two control groups without exposure (state without gingivitis and gingivitis) and three groups treatment of exposure (state of gingivitis with 1 time, 2 times, and 3 times the exposure). GCF sampling using filter paper was carried out 10 minutes after panoramic radiographic exposure. The filter paper was stained by 2% ninhydrin solution, after that the GCF volume was calculated. Results: There was a significant difference in the number of GCF (p<0.05) in the group without gingivitis and exposure compared to all other groups, the gingivitis group without exposure compared to the gingivitis group with 3 times exposure, and the gingivitis group with 1 time exposure compared to the gingivitis group 3 times with exposure. Conclusion: Panoramic radiography can cause an increase in the volume of GCF in wistar rats with gingivitis. Keywords: GCF, gingivitis, panoramic radiography

THE EFFECT OF PANORAMIC RADIOGRAPHY ON THE NUMBER OF MICRONUCLUES IN PERIODONTITIS Khairunnisa Puspita Sari; Didit Aspriyanto; Beta Widya Oktiani
Dentino : Jurnal Kedokteran Gigi Vol 6, No 2 (2021)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v6i2.11996

Background: Periodontitis is a periodontal disease that can cause an increase in Reactive Oxygen Species (ROS). The increase in ROS concentration will cause oxidative stress which has a role in DNA damage. In the case of periodontitis, panoramic radiography plays an important role in showing generalized horizontal bone loss. Radiation on panoramic radiography may cause DNA damage. DNA damage that occurs due to periodontitis or panoramic radiographic radiation exposure is characterized by the formation of micronuclei in gingival epithelial cells. Objective: To determine the effect of panoramic radiography on the number of micronuclei in Wistar rats with periodontitis. Method: This study was true experimental with post-test only and control group design. This study used 15 male Wistar rats which divided into 5 groups. Result: The average number of micronuclei in the normal group and 1 time exposures was 4 compared to the periodontitis group without exposure was 1.67, periodontitis and 1 time exposures was 8, periodontitis and 2 times the exposure was 15.67, and in the periodontitis and 3 times the exposure was 42.67. Result of One-Way Annova test and Post Hoc Bonferroni test indicated that signigicant changes in the number of micronuclei was seen between the normal group with 1 time exposure to the periodontitis group with 2 times and 3 times exposure and int the periodontitis group without exposure to the periodontitis group with 2 times and 3 times exposure. Conclusion: Panoramic radiograph X ray radiation and periodontitis can cause changes in the number of micronuclei in wistar rats. Keywords: , Micronucleus, Panoramic Radiography, Periodontitis.

HE EFFECT OF BINJAI LEAVES EXTRACT (Mangifera caesia) ON INCISIONAL BACK WOUND OF MICE (Mus musculus) TO THE NUMBER OF NEUTROPHIL CELLS (Preliminary Study of Drug Preparations for Wound Healing) Desty Ayu Dwiyanti; Irham Taufiqurrahman; Didit Aspriyanto
Dentino : Jurnal Kedokteran Gigi Vol 5, No 1 (2020)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v5i1.8116

Background: Ethanol extract of binjai leaves contain bioactive compounds in the form of flavonoid and saponin which is potential as anti-inflammatory, so it will be able to control the wound healing process. Binjai leaves extract can affect the number of neutrophil on inflammatory phase, as it is characterized by neutrophil cells infiltration to the wound tissue, so its number will increase at the beginning of inflammatory phase and decrease on the 3rd day. Objective: To prove the effect of binjai leaves extract in 1 mg/mL, 2 mg/mL, and 3 mg/mL which given topically to the number of neutrophil in day 1, 3, and 5 of wound incision on the back of male mice. Methods: The study design was a pure laboratory experimental study with post-test only with control group design which involved 36 mice divided into 4 groups: negative control group design which was given BR2 feed, treatment group which was given binjai leaves extract in 1 mg/mL, 2 mg/mL, and 3 mg/mL. Results: The result of One-way ANOVA showed that there was significant difference of all groups in day 1 (p=0.000), day 3 (p=0.001), and day 5 (p=0.000). Post-Hoc LSD test showed that there was significant difference (p<0.05) between the negative control group and treatment groups. Conclusion: Binjai leaves extract has an effect to increase the number of neutrophil in day 1 and decrease the number of neutrophil in day 3 and 5 on the healing of back wound incision in mice.

THE INFLUENCE OF TOMAN FISH (Channa micropeltes) EXTRACT ON THE NUMBER OF NEOVASCULAR IN DIABETES MELLITUS WOUND HEALING In Vivo Study on the Back of Male Wistar Rat (Rattus novergicus) Alya Royyana; Amy Nindia Carabelly; Didit Aspriyanto
Dentino : Jurnal Kedokteran Gigi Vol 3, No 2 (2018)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v3i2.5362

Background: Patients with diabetes mellitus (DM) have delayed wound healing. This happens becauseof the depletion of neovascular number. Neovascular plays an important role in wound healing by restoringblood flow to the wound area, so the new tissue gets enough oxygen and nutrition supply to proliferate. Tomanfish (Channamicropeltes) contains albumin that can increase and decrease the number of neovascular, so it’spotentially become an alternative medicine to accelerates wound healing in DM. Purpose: The aim of thisresearch to prove the effect of toman extract dose 16 ml/Kg BW rat orally on the number of neovascular inwound of diabetic wistar rats on the 4th,8th and 14th day. Materials and methods: This research used trueexperimental method with posttest only control group design. This research used 36 wistar rats which were thendivided into 9 groups, toman fish extract dose 16 ml/Kg BW of rat group, haruan fish extract dose 13,54 ml/KgBW of rat group and BR2 feed only group. Research result: The results of One Way-Anova and Post Hoc LeastSignificant Difference tests showed there was a significant difference between group of toman extract and groupof feed only, while on group of toman extract and group of haruan extract showed there was no significantdifference on 4th,8th and 14th day. The average value of toman extract-given group on 4th, 8thand 14th day were14,75. 7,5 and 6,75 respectively. Conclusion: Toman extract has effects which are increasing the number ofneovascular on 4th day and decreasing the number of neovascular on 8th and 14th day in wound healing withDM.

EFFECT OF CHANNA MICROPELTES IN THE GRANULATION, FIBROSIS AND NECROSIS OF DIABETIC WOUND HEALING Amy Nindia Carabelly; Juliyatin Putri Utami; Didit Aspriyanto; Muhammad Hasanu Reksi; Dewi Puspitasari; Priyawan Rachmadi
Dentino : Jurnal Kedokteran Gigi Vol 6, No 2 (2021)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v6i2.12001

Background: Diabetes mellitus (DM) is characterized by persistent hyperglycemia. The symptoms of DM is delayed wound healing. Delayed wound healing in diabetes will increase the risk of wound complications that will hinder the healing process, and lead to complications such as fibrosis and necrosis. The use of Channa Micropeltes (CM) extract at a dose of 16 mL/kg BW orally is proven to general optimal wound closure and wound contraction clinically in Wistar rats as diabetic model on day 14.However, the histopathological description of wound healing is yet to be known. Objective: To analyze the effect of 16 mL/kg BW dose of CM extract on histopathological changes of wound healing granulation tissue, fibrosis and necrosis in diabetic Wistar rats on day 14. Materials and Methods: This study was a true experimental design with a post-test only control group design. The samples were divided into two groups, namely the diabetic model group given CM extract at 16 mL/kg BW dose for 14 days, and the diabetic model group given BR2 feed only for 14 days. Results: The results of Mann-Whitney U test showed p = 0.003 for granulation tissue, p = 0.411 for fibrosis and p = 1 for necrosis, with the level of significance was p<0.05. Conclusion: The CM extract at 16 mL/kg BW dose affects the histopathological description of granulation tissue which presents a better result than those in the control group but did not affect the features of fibrosis and necrosis in diabetic Wistar rats on day 14. Keywords: Channa Micropeltes, Diabetic wound, Fibrosis, Granulation tissue, Necrosis.

PYTHOCHEMICAL SCREENING OF Musa acuminata STEM WATER EXTRACT Amy Nindia Carabelly; Didit Aspriyanto
Dentino : Jurnal Kedokteran Gigi Vol 5, No 1 (2020)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v5i1.8127

Background: Regenerative therapy has been progressing into the utilization of mesenchymal stem cell (MSC). Nevertheless, the limited number of MSC has put growth factor as an essential supplement for cell culture media yet relatively unaffordable because highly priced. Alternative compound which cost reasonably is required. Exogenous phytochemical material in herbal plant extract may increase the number of MSC, one of which is mauli banana stem. Purpose: To analyze secondary metabolites identified in mauli banana stem water extract. Method: Mauli banana stem was macerated using water solvent to be analyzed qualitatively for alkaloid, tannin, flavonoid, saponin, terpenoid, diterpen and steroid. Screening was followed by quantitative analysis to determine the total of alkaloid, flavonoid, condensed tannin and hydrolysable tannin. Result: Secondary metabolite compounds of mauli banana stem water extract were alkaloid (4.15%), hydrolysable tannin (1.055%), condensed tannin (0.42%) and flavonoid (0.31%). Conclusion: Mauli banana stem water extract has potential as alternative growth factor to increase the number of MSC in vitro.

THE EFFECT OF TOMAN (Channa micropeltes) FISH EXTRACT ON EPITHELIAL THICKNESS IN DIABETES MELLITUS WOUND HEALING (In Vivo Study on the back of male Wistar rat (Rattus novergicus)) Nina Audina; Amy Nindia Carabelly; Didit Aspriyanto
Dentino : Jurnal Kedokteran Gigi Vol 3, No 1 (2018)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v3i1.4614

Background: Re-epithelialization is the process of formation of new epithelium which is important in wound healing process, the faster the process then the sooner the process of wound healing. Toman fish (Channa micropeltes) is trusted as an alternative medicine to accelerate wound healing. Toman fish contains albumin which acts an antioxidant. Antioxidant can reduce Reactive Oxygen Species (ROS) which increased at state of hyperglicemia in people with diabetes mellitus (DM), Thus accelerating the process of Re epithelialization on diabetes wound. Purpose: The Purpose of this research was to prove that fish extract Toman dose 16 ml/Kg body weight effect on thickness of the epithelium of Wistar Rat Injuries Induced DM are seen on the 2, 4 and 8 day. Material and methods: This research was true experimental design with posttest-only with control design with complete random design. This research used 27 Wistar rats are divided into 9 treatment groups, Toman fish extract dose 16 mL/Kg BW, Haruan fish extract 13,54 mL/Kg BW, and BR2 feed only. The calculation of epithelial thickness was calculates using the ImageJ software application by measuring histopathological results. Research result: The result of Two way Anova test and Post Hoc LSD test showed that there was a significant difference of effect on group Toman fish extract 16 mL/Kg BW with Haruan fish extract 13,54 mL/Kg BW and BR2 feed only to increase the thickness of the wound of epithelium of Wistar rat Induced DM. Toman fish extract showed the highest mean of epithelial thickness with 2nd day (76,69 µm), 4th day (99,21 µm) and 8th day (124,23 µm). Conclusion: There was effect of Toman fish extract dose 16 mL/Kg BW on increased epithelial thickness of Wistar rats wound on induced diabetes mellitus are seen on the days 2, 4 and 8.

FGF-2 expression and the amount of fibroblast in the incised wounds of Rattus norvegicus rats induced with Mauli banana (Musa acuminata) stem extract Didit Aspriyanto; Intan Nirwana; Hendrik Setia Budi
Dental Journal (Majalah Kedokteran Gigi) Vol. 50 No. 3 (2017): September 2017
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Background: Traditional wound treatment using herbal medicine is thought to maintain the health of families and society in general economically, effectively, and efficiently without inducing side effects. One genus of plant that can be used as a traditional medicine is the Mauli banana, indigenous to South Borneo. Mauli banana stem contains bioactive compounds, most of which are tannins along with ascorbic acid, saponin, β-carotene, flavonoids, lycopene, alkaloids, and flavonoids. Tanin has antibacterial and antioxidant effects at low concentrations, as wells as antifungal ones at high concentrations. Purpose: This study aimed to analyze the effects of Mauli banana stem extract at concentrations of 25%, 37.5%, and 50% on the quality of incised wound healing in male Rattus norvegicus rats by assessing FGF-2 expression and fibroblast concentration on days 3 and 7. Methods: This research represented an experimental laboratory-based investigation involving 32 rats of the Rattus norvegicus strain aged 2-2.5 months old. Sampling was performed using a simple random sampling technique since the research population was considered homogeneous and divided into 8 treatment groups (C3, M3-25, M3-37.5, M3-50, C7, M7-25, M7-37.5, M7-50). The rats in each group were anesthetized before their back was incised with length and width of 15x15mm with a depth of 2mm. Gel hydroxy propyl cellulose medium (HPMC) was applied to the incised wound of each rat in the control group, while stem Mauli banana extract was applied to that of each rat in the treatment groups three times a day at an interval of 6-8 hours. On day 3, four rats from each group were sacrificed, while, in the remaining groups, the same procedure was performed until day 7, at which point they (8 groups) were sacrificed for HE examination in order to assess the amount of fibroblast and for IHC examination to examine FGF-2 expression. Data regarding FGF-2 expression and the amount of fibroblast were analysed by means of One-way Anova and HSD. Results: The results showed that the Mauli banana stem extract could significantly improve the expression of FGF-2 and the amount of fibroblast cells compared to C3 and C7 groups. The highest increase in FGF-2 expression and fibroblast amount were found in all groups on days 3 and 7 treated with the Mauli banana stem extract at a concentration of 50%. Conclusion: There was an increase of FGF-2 expression and the amount of fibroblast cells in the incision wound healing process that induced with Mauli banana stem extract.

COMPARISON OF SALIVARY pH LEVEL BETWEEN DOWN SYNDROME AND NON-DOWN SYNDROME (NORMAL) PATIENTS (Case Review in Martapura, Banjar Regency) Rudie Syahrizal Akhmad; Rosihan Adhani; Didit Aspriyanto
Dentin Vol 3, No 3 (2019)
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar

ABSTRACTBackground : Down syndrome is a genetic chromosomal abnormality that causes disorder in the motor, sensory and cognitive systems, also difficulty in maintaining oral hygiene to the sufferers. However, the prevalence of dental caries in Down syndrome patients is actually lower than Non-Down Syndrome individuals. This is thought to be due to the influence of the higher salivary pH of Down syndrome patients. Objective: To find out the ratio of salivary pH between people with Down Syndrome and Non-Down Syndrome individuals in Special Schools in Martapura, Banjar Regency. Methods: This study used an observational analytic method with a Cross Sectional approach. The sampling method was total sampling of all Down syndrome patients in Special Schools in Martapura, Banjar Regency and non-Down Syndrome respondents using simple random sampling, were taken randomly in elementary, middle and high school in Martapura. Measurement of salivary pH was carried out using a digital pH meter. Results: The results showed a significant difference between the salivary pH level in People with Down Syndrome and Non-Down Syndrome. The salivary pH level of Non-Down Syndrome was lower than ones with Down syndrome. The average value of the Non-Down Syndrome salivary pH level was 6.19 and Down syndrome was 7.24. Conclusion: Salivary pH of patients with Down Syndrome is higher than the Non-Down Syndrome individual which means the salivary pH in people with Down Syndrome are more alkalic than Non-Down Syndrome (Normal) individual.Keywords: Dental Caries, Down Syndrome, pH, Saliva

Co-Authors A'idah, Nurul Adhimas Rilo Pambudi Ahda Ahda Annisa Aini, Lisa Shofa’ Nur Aliffia Azizah Kawiadji Alya Royyana Amy Nindia Carabelly Arifin, Rahmad At-Thoyyar, Aila Audina, Nina Aulia Azizah Azaria, Nisa Bayu Indra Sukmana Bayu Yordha Senggara Beta Widya Oktiani Brachmedio Barito Syech Erlangga Debby Saputera, Debby Desty Ayu Dwiyanti Dewi Nurdiana Dewi Puspitasari Dewi Puspitasari Dian Dwiyanti Dita Puspita Sari Diza Afira Hutasuhut Dwi Kurniawan, Fajar Kusuma Dwiyanti, Dian Eny Febriyanti Erika Norfitriyah Firda Damayanti Fitri Siregar, Eka Dwita Natasya Helda Helda Hendrik Setia Budi I Wayan Arya Krishnawan Firdaus Ichrom Nahzi, Muhammad Yanuar Ihsan, Muhammad Fauzan Ika Kusuma Wardani Intan Nirwana, Intan Jatmiko, Habibi Naufal Khairunnisa Puspita Sari Krishnawan Firdaus, I Wayan Arya Maulana, Noval Ihza Maydina Izzatul Yazidah Milka Widya Sari Muhammad Hasanu Reksi Muhammad Yunanda Anhar Nadia Chairina Nahzi, Yanuar Ichrom Naila Khairiyah Nina Audina Nor Rahmatika, Aniqah Norfitriyah, Erika Novi Tiara Lestari Nurlailatul Rahmah Nurrahman, Tri Nurul Fathanah Putri Oktiani, Beta Widya Prilly Sonya Puteri Priyawan Rachmadi Putra Ramadhani, Krisna Erlangga Ramadhaniyah, Nur Ramadhanty, Aina Renie Kumala Dewi Rini Rahmiyati Rosdayatri, Nadhira Agnia Rosihan Adhani, Rosihan Rudie Syahrizal Akhmad Sari, Milka Widya Sarifah, Norlaila Senggara, Bayu Yordha Setyawardhana, R. Harry Dharmawan Setyawardhana, Raden Harry Dharmawan Sherli Diana Syifa Ennisa Tri Putri, Deby Kania US Ramadilla Ramadilla Utami, Juliyatin Putri Wydiamala, Erida Yusrinie Wasiaturrahmah

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