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All Journal ODONTO Dental Journal Majalah Obat Tradisional Dentino: Jurnal Kedokteran Gigi Dental Journal (Majalah Kedokteran Gigi) Dentin Jurnal Radiologi Dentomaksilofasial Indonesia (JRDI) Odonto dental journal Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia
Aspriyanto, Didit
Department Of Dentomaxillofacial Radiology, Faculty Of Dentistry, Universitas Lambung Mangkurat, Banjarmasin, Indonesia, 70236
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Dentistry Energy Environmental Science Health Professions Medicine & Pharmacology Physics Public Health

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ANTIBACTERIAL ACTIVITY OF RAMBAI LEAF EXTRACT (Sonneratia caseolaris) CONCENTRATION 25% 50% 75% AND 100% AGAINST BACTERIA Enterococcus faecalis US Ramadilla Ramadilla; Didit Aspriyanto; Sherli Diana
Dentino : Jurnal Kedokteran Gigi Vol 8, No 1 (2023)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v8i1.16076

Abstract

ABSTRACTBackground: The causative factor of failure in the treatment of the root canal is the presence of microorganisms, namely the bacterium Enterococcus faecalis. Rambai leaf extract (Sonneratia caseolaris) also contains secondary metabolite compounds, namely, triterpenoids, phenols, tannins, steroids, and flavonoids that are able to inhibit the growth of Enterococcus faecalis bacteria which have the potential to cause failure in root canal treatment. Purpose: This research has a purpose to see the antibacterial activity of Rambai leaf extract (Sonneratia caseolaris) concentrations of 25%, 50%, 75%, and 100% against Enterococcus faecalis bacteria. Method: This study is a true experimental study designed by Posttest Only with Control Group Design using six. Material and methods: Rambai leaf extract concentrations of 25%, 50%, 75%, 100%, positive control of chlorhexidine gluconate 2%, and negative control of aqua dest was repeated 4 times. Antibacterial activity can be seen from the magnitude of the inhibitory zone formed in (MHA) Mueller Hinton Agar and BHIB (Brain Heart Infusion) using the diffusion method. Results: Based on Mann Whitney's final test, there was a significant difference in the clear zones formed around the paper disk. The average clear zone of Rambai leaf extract (Sonneratia caseolaris) concentration is 25% at 10.0mm, 50% at 12.7mm, 75% at 17.0mm, and 100% at 18.3mm. Conclusion: There is activity of Rambai leaf extract (Sonneratia caseolaris) concentrations of 25%, 50%, 75%, and 100% against Enterococcus faecalis bacteria. Keywords: Antibacterial Activity, Enterococcus faecalis, Rambai Leaf Extract
ANTIBACTERIAL EFFECTIVENESS OF KECAPI SENTUL EXTRACT (SANDORICUM KOETJAPE MERR.) AGAINST STREPTOCOCCUS MUTANS Adhimas Rilo Pambudi; Yusrinie Wasiaturrahmah; Didit Aspriyanto
Odonto : Dental Journal Vol 8, No 2 (2021): December 2021
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (278.864 KB) | DOI: 10.30659/odj.8.2.1-10

Abstract

ABSTRACTBackground: One of the causing dental caries is a microorganism, namely Streptococcus mutans. Kecapi sentul leaves extract (Sandoricum koetjape Merr.) contain alkaloid, flavonoid, saponin, steroid, phenol, and triterpenoid which have antibacterial properties on the inhibition Streptococcus mutans which has the potential to prevent dental caries.Method: This research uses a laboratory experimental design with a post-test control group only design, using seven treatment groups, namely kecapi sentul leaves extract with the concentration of group 30%, 40%, 50%, 60%, 70%, Chlorhexidine gluconate 0.2% as positive control and aquadest as negative control were repeated 4 times. Result: Non parametric test Kruskal Wallis and Post Hoc Mann Whitney methods showed that each treatment group was significantly different in the diameter of the formed inhibition zone. The mean diameter of the inhibition zone with a concentration of 30% was 9.1 mm, 40% was 13.3 mm, 50% was 17.13 mm, 60% was 18.65 mm and 70% was 21.05 mm.Conclusion: Kecapi sentul leaves extract (Sandoricum koetjape Merr.) with the concentration of group 30%, 40%, 50%, 60% and 70% have antibacterial potential against the growth of Streptococcus mutans.
Differences in Panoramic Mandibular Index value between male and female hypertension patients using panoramic radiography Novi Tiara Lestari; Norlaila Sarifah; Didit Aspriyanto; Bayu Indra Sukmana; Isyana Erlita
Jurnal Radiologi Dentomaksilofasial Indonesia (JRDI) Vol 7 No 2 (2023): Jurnal Radiologi Dentomaksilofasial Indonesia (JRDI)
Publisher : Ikatan Radiologi Kedokteran Gigi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32793/jrdi.v7i2.1065

Abstract

Objectives: This study is aimed to analyze the panoramic mandibular index value in male and female hypertensive patients using panoramic radiography. Materials and Methods: This research is a comparative analytic research with a purposive sampling technique. The study sample was hypertensive patients at Ulin Hospital, Banjarmasin from March to May 2023. Results: The results showed that there was no statistically significant difference through statistical data on the panoramic mandibular index value between men (0.27 ± 0.07) and women (0.26 ± 0.09), The age group that experienced a decrease in mandibular cortical thickness was most in the age group ≥ 65 years (0.22 ± 0.06). Conclusion: It is known that there is no significant difference in the panoramic mandibular index value between women and men.
ANTIBACTERIAL ACTIVITY OF RAMBAI LEAF EXTRACT (Sonneratia caseolaris) CONCENTRATION 25%, 50%, 75%, AND 100% AGAINST Streptococcus sanguinis Nurul Fathanah Putri; Didit Aspriyanto; Isyana Erlita
Dentino : Jurnal Kedokteran Gigi Vol 8, No 2 (2023)
Publisher : FKG Unlam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v8i2.17520

Abstract

Background: Dental plaque is a soft layer formed by collection of bacteria that is firmly attached to the tooth surface which if left unchecked can cause tooth decay, periodontal disease and dental caries. Streptococcus sanguinis is the first bacteria to form colonization in plaque formation. Plaque can be diminished by using chlorhexidine gluconate 0,2% mouthwash, but in long-term use it can cause side effects, so an alternative herbal-based mouthwash that has minimal side effects is needed. Rambai leaf extract can be used as an alternative mouthwash because it contains antibacterial compounds, namely, flavonoids, tannins, phenols, triterpenoids, and steroids.  Purpose: The purpose of this study was to determine the antibacterial activity of rambai leaf extract at concentrations of 25%, 50%, 75% and 100% against Streptococcus sanguinis bacteria. Methods: This study used true experimental research with post-test only with a control group design using rambai leaf extract 25%, 50%, 75%, 100%, chlorhexidine gluconate 0.2%, and aquadest. The study used 6 groups with 4 repetitions with a total of 24 samples. Each group was incubated for 24 hours at 37°C, then the diameter of the inhibition zone was measured using a calliper. Results: The results of the Mann Whitney test showed that there was a significant difference between each treatment group and there was no significant difference between 100% rambai leaf extract and chlorhexidine gluconate 0.2%. The average diameter of the inhibition zone of rambai leaf extract was 25% by 9.75 mm, 50% by 13.37 mm, 75% by 16.12 mm, 100% by 19.5 mm, chlorhexidine gluconate 0.2% by 19.12 mm and aquadest 0 mm. Conclusion: Rambai leaf extract with concentrations of 25%, 50%, 75%, and 100% had antibacterial activity against Streptococcus sanguinis. Keywords :  Antibacterial Activities, chlorhexidine gluconate 0,2%,  rambai leaves extracts, Streptococcus sanguinis
UJI TOKSISITAS EKSTRAK DAUN GALAM (Melaleuca cajuputi subsp. Cumingiana Barlow) PADA BHK-21 SEL FIBROBLAS Brachmedio Barito Syech Erlangga; Sherli Diana; Debby Saputera; Didit Aspriyanto; Beta Widya Oktiani
Dentin Vol 7, No 3 (2023)
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentin.v7i3.10743

Abstract

Latar Belakang: Karies gigi merupakan penyakit rongga mulut yang salah satu faktor penyebabnya adalah bakteri. Salah satu bahan alam yang berpotensi sebagai antibiotik dan hidup di lingkungan lahan basah adalah tanaman galam (Melaleuca cajuputi subsp. Cumingiana Barlow). Fungsi uji toksisitas adalah untuk mengetahui efek toksik dan batas dosis aman suatu senyawa kimia dalam penelitian ini pengujian ekstrak daun galam. Tujuan: Menganalisis efek toksik setelah pemberian ekstrak daun galam (Melaleuca cajuputi subsp. Cumingiana Barlow) terhadap sel fibroblas BHK-21. Metode: Penelitian ini adalah penelitian eksperimental murni (true experimental) dengan rancangan posttest-only with control group design untuk menganalisis toksisitas ekstrak daun galam terhadap sel fibroblas Baby Hamster Kidney-21 (BHK-21) dengan metode Microculture Tetrazolium Technique (MTT) assay secara in vitro. Hasil: Hasil penelitian menunjukkan bahwa ekstrak daun galam tidak toksik karena viabilitas sel pada semua konsentrasi >100% dan nilai IC50 yang tidak mungkin tercapai. Hasil uji post hoc Games-Howell menyimpulkan bahwa konsentrasi 50%, 75%, dan 100% lebih efektif daripada konsentrasi 0,125%, 0,2%, 0,25%, dan 0,4%. Kesimpulan: Tidak ada efek toksik ekstrak daun galam dengan uji MTT terhadap sel fibroblas BHK-21.Kata kunci: ekstrak daun galam, sel fibroblas BHK-21, toksisitas
PENGARUH EKSTRAK DAUN KARAMUNTING (Rhodomytrus tomentosa) TERHADAP KADAR TROMBOSIT SETELAH PAPARAN SINAR-X RADIOGRAFI PERIAPIKAL Ahda Ahda Annisa; Didit Aspriyanto; Nurdiana Dewi; I Wayan Arya Krishnawan Firdaus; Debby Saputera
Dentin Vol 7, No 3 (2023)
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentin.v7i3.10748

Abstract

Latar Belakang : Paparan radiasi sinar-X radiografi periapikal dapat merusak sel darah salah satunya yaitu trombosit dengan cara membentuk radikal bebas sehingga dapat terjadinya penurunan pada sel darah. Kandungan oleh ekstrak daun karamunting dapat menangkal radikal bebas dan meningkatkan sel darah dari paparan radiasi sinar-X radiografi periapikal. Senyawa metabolit sekunder pada daun karamunting memiliki tiga kandungan senyawa tertinggi yaitu flavonoid, phenol dan tanin. Tujuan: Menganalisis pengaruh ekstrak daun karamunting terhadap kadar trombosit mencit jantan setelah paparan sinar-X radiografi periapikal. Metode : Penelitian ini menggunakan metode true experimental dengan rancangan post test only with control group design menggunakan 30 ekor mencit dibagi menjadi 5 kelompok. Kelompok K- adalah diberikan ekstrak daun karamunting tetapi tidak diberikan paparan radiasi sinar-X, kelompok K+ adalah diberikan radiasi sinar-X radiografi periapikal tanpa diberikan ekstrak daun karamunting, kelompok P1 adalah diberikan ekstrak daun karamunting dan paparan radiasi sinar-X radiografi periapikal pengulangan 1 kali, kelompok P2 adalah diberikan ekstrak daun karamunting dan paparan radiasi sinar-X radiografi periapikal pengulangan 7 kali dan kelompok P3 adalah diberikan ekstrak daun karamunting dan paparan radiasi sinar-X radiografi periapikal pengulangan 10 kali. Hasil : Hasil uji Post Hoc Bonferoni menunjukan perbedaan bermakna antar kelompok kontrol dan kelompok perlakuan. Kesimpulan : Terdapat pengaruh kadar trombosit pada mencit jantan (Mus musculus) pemberian ekstrak daun karamunting setelah paparan sinar-X radiografi periapikal. Kata kunci : Ekstrak daun karamunting, Radiografi periapikal, trombosit
PERBANDINGAN PENGETAHUAN KESEHATAN GIGI DAN MULUT ANTARA PENGGUNAAN APLIKASI HI BOGI DAN SIMANGGIS CELEBES Muhammad Yunanda Anhar; R. Harry Dharmawan Setyawardhana; Nurdiana Dewi; Aulia Azizah; Didit Aspriyanto
Dentin Vol 7, No 3 (2023)
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentin.v7i3.10741

Abstract

Latar Belakang: Pentingnya menjaga kebersihan gigi dan mulut dapat ditingkatkan dengan menambah pengetahuan dalam menjaga kesehatan gigi dan mulut. Salah satu cara meningkatkan pengetahuan kesehatan gigi dan mulut yaitu dengan mengakses aplikasi HI BOGI dan SIMANGGIS CELEBES sebagai aplikasi digital yang memberikan informasi mengenai pentingnya menjaga kesehatan gigi dan mulut. Tujuan : Menganalisis perbandingan penggunaan aplikasi HI BOGI dan SIMANGGIS CELEBES terhadap pengetahuan Kesehatan gigi dan mulut pada siswa kelas III SDN Kuripan 2 Banjarmasin. Metode : Penelitian ini menggunakan metode Quasi Experimental dengan rancanga pretest dan posttest design, teknik pengambilan sampel Total sampling. Penelitian dilaksanakan di SDN Kuripan 2 Banjarmasin dengan sampel berjumlah 58 siswa. Hasil : Hasil uji wilcoxon pengetahuan pada kelompok HI BOGI didapatkan 0,000 <0,05. Hasil uji wilcoxon pengetahuan pada kelompok SIMANGGIS CELEBES didapatkan 0,000 <0,05. Hasil uji Mann Whitney pada aplikasi HI BOGI dan SIMANGGIS CELEBES didapat 0,033 <0,05. Kesimpulan : terdapat perbedaan peningkatan pengetahuan kesehatan gigi dan mulut antara aplikasi HI BOGI dan SIMANGGIS CELEBES pada siswa kelas III SDN Kuripan 2 Banjarmasin usia 8-9 Tahun. Kesimpulan: Terdapat perbedaan peningkatan pengetahuan kesehatan gigi dan mulut antara aplikasi HI BOGI dan SIMANGGIS CELEBES. Kata kunci : Aplikasi HI BOGI, Aplikasi SIMANGGIS CELEBES, Kesehatan Gigi dan Mulut, Teledentistry
ANTIBACTERIAL ACTIVITY OF PAPUYU FISH SCALE CHITOSAN (Anabas testudineus) AGAINST Staphylococcus aureus At-Thoyyar, Aila; Ichrom Nahzi, Muhammad Yanuar; Puspitasari, Dewi; Aspriyanto, Didit; Utami, Juliyatin Putri
Dentino: Jurnal Kedokteran Gigi Vol 9, No 2 (2024)
Publisher : FKG ULM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v9i2.20399

Abstract

Backgrounds: Pulp disease due to caries can be treated through endodontic treatment, which aims to eliminate bacteria in the root canal so microorganisms cannot multiply. Staphylococcus aureus is a resistant microorganism causing root canal treatment failure. Chlorhexidine as a gold standard cannot remove biofilm and other organic debris. Utilization of nature, chitosan of papuyu fish scales (Anabas testudineus) has antibacterial properties that can be an alternative root canal irrigation material. Purpose: Analyze the antibacterial activity of chitosan of papuyu fish scales against Staphylococcus aureus based on the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). Methods: This research is a true experimental design with posttest only with control group design. The research groups were chitosan of Papuyu fish scales with concentrations of 25%, 50%, 75%, 100%, chlorhexidine gluconate 2% (positive control), and aquadest (negative control) with 6 repetitions. Results: The MIC value in the One-Way ANOVA test showed a significant difference (p<0.05). Data analysis followed by Post Hoc Games-Howell test showed all treatment groups had significant differences between each other (p<0.05). The MBC value in the Kruskal Wallis test shows that there is a significant difference (p<0.05). Data analysis followed by MannWhitney test showed significant differences between each other (p<0.05) except for the group of papuyu fish scale chitosan 25% concentration against papuyu fish scale chitosan 50% concentration, papuyu fish scale chitosan 100% concentration against chlorhexidine gluconate 2% (p>0.05). Conclusion: There is a difference in the antibacterial activity of chitosan of Papuyu fish scales concentrations of 25%, 50%, 75%, 100%, chlorhexidine gluconate 2% (positive control) and aquadest (negative control) against Staphylococcus aureus bacteria.
THE EFFECT OF KASTURI (Mangifera Casturi) BARK EXTRACTS AT CONCENTRATION OF 12.7% ON THE NUMBER OF MACROPHAGES AFTER TOOTH EXTRACTION Rosdayatri, Nadhira Agnia; Aspriyanto, Didit; Taufiqurrahman, Irham
Dentino: Jurnal Kedokteran Gigi Vol 9, No 1 (2024)
Publisher : FKG ULM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v9i1.18854

Abstract

Background: Tooth extraction is a surgical procedure to take part of the tooth that exposes the bone and soft tissues of the oral cavity. The wound will occur in the soft tissue, and hard tissue around the extraction area after the tooth extraction. Wound healing after tooth extraction begins with the inflammatory phase, in which the movement of leukocytes occurs, such as neutrophil cells. If the wound is chronic, it will be replaced by macrophages. Macrophages are the dominant cells in chronic inflammation and have a role in phagocytizing bacteria and clean damaged tissue. However, if the disturbance occurs during the wound-healing phase, it can cause bleeding, swelling, and infection, so that a safe alternative medicine is required, which is from an herbal plant of Kasturi bark extracts at a concentration of 12.7%. Objective: To prove the effect of the administration of Kasturi (Mangifera casturi) bark extracts at a concentration of 12.7% applied topically to the number of macrophage cells in the tooth extraction wound of Wistar rats (Rattus novergicus). Methods: This study was an Experimental Laboratories research with Post Test with Control Group Design. This study was performed on 24 Wistar rats that had been adapted ±2 weeks with a simple random sampling technique. The calculation of macrophage cells was conducted through direct observation with a light microscope and 3 visual fields. Results: The results of the study showed that Kasturi bark extracts had more effect on the number of macrophage cells than control groups. The results of One Way ANOVA showed that there is a significant relationship between each group (p<0.05). Conclusion: Kasturi bark extracts at a concentration of 12.7% can help the number of macrophage cells increase on the 3rd day and decrease on the 5th day after tooth extraction of Wistar rats. Keywords: Kasturi Bark Extract, Macrophage, Tooth Extraction.
ANTIBACTERIAL EFFECTIVENESS OF RAMBAI (Sonneratia Caseolaris) LEAVES EXTRACT AGAINST STREPTOCOCCUS MUTANS Nor Rahmatika, Aniqah; Aspriyanto, Didit; Ichrom Nahzi, Muhammad Yanuar
Dentino: Jurnal Kedokteran Gigi Vol 9, No 1 (2024)
Publisher : FKG ULM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v9i1.18859

Abstract

Background: The 2018 Riskesdas data stated that dental caries prevalence in Indonesia was 88.8%. Dental caries is a multifactorial disease characterized by hard tissue damage to the enamel, dentin, and cementum of the teeth. One of the causes of caries is the presence of a microorganism called Streptococcus mutans. Rambai leaf extract (Sonneratia caseolaris) is a typical plant of South Kalimantan with content flavonoids, tripernoids, tannins, phenols, steroids that have antibacterial properties against the growth of Streptococcus mutans which can prevent caries. Purpose: To determine the difference in the effectiveness of the antibacterial extract of rambai leaves (Sonneratia caseolaris) against bacteria Streptococcus mutans in vitro. Methods: This research was a true experimental with a post-test-only design and a control group, using six treatment groups, namely 20%, 40%, 60%, 80%, positive control (povidone iodine 1%), and negative control (aquadest) respectively. Each treatment got 3 repetitions. The antibacterial test method used in this study was the liquid dilution method to determine the Minimun Inhibitory Concentration (MIC) by using Spectrophotometer and solid dilution to determine the Minimum Bactericidal Concentration (MBC) by using tools Colony Counter. Results: Based on the results and data analysis, it can be seen that the rambai leaf extract has a MIC of 20% and a MBC of 60%. Conclusion: Rambai leaf extract (Sonneratia caseolaris) can inhibit and have antibacterial activity against the growth of Streptococcus mutans. Keywords: Antibacterial, Rambai leaf extract, Streptococcus mutans
Co-Authors Adhimas Rilo Pambudi Agung Satria Wardhana Ahda Ahda Annisa Aliffia Azizah Kawiadji Alya Royyana Amy Nindia Carabelly Arifin, Rahmad At-Thoyyar, Aila Audina, Nina Aulia Azizah Bayu Indra Sukmana Bayu Yordha Senggara Beta Widya Oktiani Brachmedio Barito Syech Erlangga Debby Saputera, Debby Desty Ayu Dwiyanti Dewi Nurdiana Dewi Puspitasari Dewi Puspitasari Dian Dwiyanti Diana Wibowo Dita Puspita Sari Diza Afira Hutasuhut Dwi Kurniawan, Fajar Kusuma Dwiyanti, Dian Eka Dwita Natasya Fitri Siregar Eny Febriyanti Erida Wydiamala Erika Norfitriyah Firda Damayanti Helda Helda Hendrik Setia Budi I Wayan Arya Krishnawan Firdaus Ichrom Nahzi, Muhammad Yanuar Ihsan, Muhammad Fauzan Ika Kusuma Wardani Intan Nirwana, Intan Jatmiko, Habibi Naufal Khairunnisa Puspita Sari Krisna Erlangga Putra Ramadhani Lisa Shofa’ Nur Aini Maydina Izzatul Yazidah Milka Widya Sari Muhammad Hasanu Reksi Muhammad Yunanda Anhar Nadia Chairina Nahzi, Yanuar Ichrom Naila Khairiyah Nina Audina Nisa Azaria Nor Helma Warni Nor Rahmatika, Aniqah Norfitriyah, Erika Noval Ihza Maulana Nurlailatul Rahmah Nurrahman, Tri Nurul A&#039;idah Nurul Fathanah Putri Oktiani, Beta Widya Prilly Sonya Puteri Priyawan Rachmadi Raket Rizki Rahmaningtyas Ramadhaniyah, Nur Ramadhanty, Aina Renie Kumala Dewi Rima Permata Sari Rini Rahmiyati Rosdayatri, Nadhira Agnia Rosihan Adhani, Rosihan Rudie Syahrizal Akhmad Sari, Galuh Dwinta Sari, Milka Widya Sarifah, Norlaila Senggara, Bayu Yordha Setyawardhana, Raden Harry Dharmawan Sherli Diana Syifa Ennisa Timothy Jogy Sotarduga Parhusip Tri Putri, Deby Kania US Ramadilla Ramadilla Utami, Juliyatin Putri Widodo Widodo Yusrinie Wasiaturrahmah