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Anti-inflammatory Effects of Channa micropeltes Extract through NF-κB and TNF-α in Diabetic Rat Carabelly, Amy Nindia; Aspriyanto, Didit; Sari, Milka Widya; Norfitriyah, Erika; Utami, Juliyatin Putri
Majalah Obat Tradisional Vol 29, No 1 (2024)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mot.87104

Diabetes mellitus (DM) is a disorder of carbohydrate, fat, and protein metabolism caused by impaired insulin secretion, insulin action, or both characterized by hyperglycemia. Hyperglycemic condition is associated with inflammation process leading to complications. Food supplements play an important role in controlling protein metabolism and have anti-inflammatory properties such as Channa micropeltes (CM). This study aimed to analyze the effect of CM extract on the levels of molecular markers Nuclear factor kappa beta (NF-κB) and Tumor Necrosis Factor alfa (TNF-α) in normal and diabetic rats. The experimental design of this investigation was a post-test-only control group design and the samples were the diabetic model group given CM extract at 16 mL/kgBB dose, and negative control group for 14 days, respectively. Serum concentrations of NF-κB and TNF-α were measured by ELISA. DM-CM group showed decreasing levels of NF-κB. TNF- α was detected with the lowest level in the N-CM group. The levels of TNF- α declined close to normal levels in the DM-CM group. Hence, the conclusion was the CM at a dose of 16 mL/kg BW for 14 days in Wistar rats can reduce Nf-κB and TNF-α in groups of normal and diabetic rats.

PHARMACOLOGICAL INVESTIGATION OF THE ORAL WOUND HEALING ACTIVITY OF Stachytarpheta jamaicensis ROOT EXTRACT GEL IN WISTAR RATS Utami, Juliyatin Putri; Wasiaturrahmah, Yusrinie; Carabelly, Amy Nindia; Aspriyanto, Didit; Nahzi, Yanuar Ichrom; Arifin, Rahmad; Ramadhaniyah, Nur; Jatmiko, Habibi Naufal
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 1 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.9390

The Stachytarpheta jamaicensis (L) Vahl root contains bioactive compounds like saponins, alkaloids, flavonoids, tannins, phenols, and terpenoids, which have antibacterial and anti-inflammatory properties. This study investigates the wound healing effects of Stachytarpheta jamaicensis (L) Vahl root extract gel in oral mucosa. The experiment involved 28 male Wistar rats, divided into four groups: a positive control treated with povidone iodine 10%, a negative control treated with gel base, and two experimental groups treated with 6% and 10% S. jamaicensis root extract gels. The gel was applied twice daily, and wound healing was assessed by epithelial thickness, inflammation cell and hydroxyproline content. Both concentrations of S. jamaicensis root extract gel significantly enhanced wound healing, as shown by increased epithelial thickness, inflammation cell and hydroxyproline content levels compared to the negative control. These findings suggest that S. jamaicensis root extract gel effectively promotes wound healing in the excision wound model.

PHARMACOLOGICAL INVESTIGATION OF THE ORAL WOUND HEALING ACTIVITY OF Stachytarpheta jamaicensis ROOT EXTRACT GEL IN WISTAR RATS Utami, Juliyatin Putri; Wasiaturrahmah, Yusrinie; Carabelly, Amy Nindia; Aspriyanto, Didit; Nahzi, Yanuar Ichrom; Arifin, Rahmad; Ramadhaniyah, Nur; Jatmiko, Habibi Naufal
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 1 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.9390

The Stachytarpheta jamaicensis (L) Vahl root contains bioactive compounds like saponins, alkaloids, flavonoids, tannins, phenols, and terpenoids, which have antibacterial and anti-inflammatory properties. This study investigates the wound healing effects of Stachytarpheta jamaicensis (L) Vahl root extract gel in oral mucosa. The experiment involved 28 male Wistar rats, divided into four groups: a positive control treated with povidone iodine 10%, a negative control treated with gel base, and two experimental groups treated with 6% and 10% S. jamaicensis root extract gels. The gel was applied twice daily, and wound healing was assessed by epithelial thickness, inflammation cell and hydroxyproline content. Both concentrations of S. jamaicensis root extract gel significantly enhanced wound healing, as shown by increased epithelial thickness, inflammation cell and hydroxyproline content levels compared to the negative control. These findings suggest that S. jamaicensis root extract gel effectively promotes wound healing in the excision wound model.

TOXICITY TEST OF KELAKAI LEAF EXTRACT (Stenochlaena palustris) TOWARD WISTAR RATS LIVER (Rattus norvegicus) Krisna Erlangga Putra Ramadhani; I Wayan Arya Krishnawan Firdaus; Yusrinie Wasiaturrahmah; Didit Aspriyanto; Erida Wydiamala
Dentino: Jurnal Kedokteran Gigi Vol 10, No 1 (2025)
Publisher : FKG ULM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v10i1.22200

Background: Kelakai leaf extract can be used as an alternative wound healing medicine because it contains flavonoid and phenolic compounds that act as antioxidants. Before use, herbal plants must ensure the level of safety that can be obtained from toxicity tests, one of which is in vivo which can be seen from their effect on the liver of Wistar rats based on SGOT and SGPT levels. Purpose: Proving there is no toxic effect from the administration of kelakai leaf extract doses of 2,000, 2,500, and 3,000 mg/kgBW on the liver of Wistar rats based on SGOT and SGPT levels. Method: Pure laboratory experimental research with posttest-only design with control group design, consisting of 16 wistar rats divided into 4 groups with 1 negative control group given distilled water and 3 treatment groups given doses of kelakai leaf extract 2,000, 2,500 and 3,000 mg/kgBW twice a day every morning and evening for 28 days. Results: SGOT and SGPT levels after 28 days were still in the normal range. SGOT levels in treatment groups 1, 2, and 3 were 61.244 U/L, 58.953 U/L, and 53.536 U/L. SGPT levels in treatment groups 1, 2 and 3 were 25.137 U/L, 23.331 U/L, and 21.179 U/L. Based on statistical tests, there were significant differences in all treatment groups. Conclusion: There is no toxic effect from the administration of kelakai leaf extract doses of 2,000, 2,500, 3,000 mg/kgBW orally for 28 days on the liver of Wistar rats based on SGOT and SGPT levels.

TOXICITY TEST OF Eusideroxylon zwageri BARK EXTRACT ON LIVER HISTOPATHOLOGY PARENCHYMATOUS DEGENERATION AND FATTY DEGENERATION Nurul A'idah; Didit Aspriyanto; I Wayan Arya Krishnawan Firdaus; Bayu Indra Sukmana; Erida Wydiamala
Dentino: Jurnal Kedokteran Gigi Vol 10, No 1 (2025)
Publisher : FKG ULM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v10i1.22203

Background: Ironwood bark extract can be developed into an alternative herbal medicine for wound healing because it contains phenolics, flavonoids, and proanthocyanidins which can act as an antioxidant. Before being used as an herbal medicine, it is necessary to ensure its safety through an toxicity test. Purpose: To find out whether there is no toxic effect on the orally administration of ironwood bark extract (Eusideroxylon zwageri) at the doses of 1,250 mg/kgBW, 2,750 mg/kgBW, and 4,750 mg/kgBW to the livers of Wistar rats (Rattus norvegicus) based on histopathological appearance of parenchymatous degeneration and fatty degeneration. Methods: This research is purely experimental with a posttest only with control design. The sample in this study were 16 Wistar rats which were divided into 4 groups, namely the control group which was only administered distilled water and the treatment groups P1, P2, P3 which were administered ironwood bark extrac at the doses of 1,250 mg/kg BW, 2,750 mg/kg BW, and 4,750 mg/kgBW of 2x1 ml every 24 hours for 14 days. Results: The average percentages of histopathological appearance of parenchymatous degeneration and fatty degeneration in the K, P1, P2, and P3 groups showed a score of 0 which was categorized as normal. Data analysis showed that there were no significant differences between groups P1, P2, and P3 with the control group. Conclusion: Ironwood bark extract doses of 1,250 mg/kg BW, 2,750 mg/kg BW, and 4,750 mg/kg BW had no toxic effect on the liver of Wistar rats based on histopathological appearance of parenchymatous degeneration and fatty degeneration.

MEASUREMENT VALUE OF BANJAR ETHNIC MANDIBULAR INCISOR POSITION USING LATERAL CEPHALOMETRIC RADIOGRAPH Nor Helma Warni; Didit Aspriyanto; Agung Satria Wardhana; Isyana Erlita; Ika Kusuma Wardani
Dentin Vol 9, No 2 (2025)
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentin.v9i2.17744

ABSTRACTBackground: Appearance is one of the most important things for everyone in this modern era. Malocclusion, also known as tooth-jaw disharmony, can lead to a decline in a person's appearance. The position and inclination of the anterior teeth must be favorable to ensure maximum facial harmony. One common effort to achieve maximum facial harmony is orthodontic treatment. To support this treatment, x-rays are needed, one of which is the cephalometric technique with Steiner analysis. Purpose: This study aims to determine the measurement value of the mandibular incisor position of Banjarese students of SMAN 12 Banjarmasin from the lateral cephalometric radiograph using Steiner analysis. Method: Cephalometric measurements were carried out using the Steiner analysis method on the dental landmarks; L1-NB. Data analysis was carried out using descriptive statistical methods. The sample in this study was secondary data from previous research and took pure Banjarese students of SMAN 12 Banjarmasin, characterized by at least two generations (father, mother, grandfather, and grandmother) who had never or were not undergoing orthodontic treatment and were willing to be subjects in the study. Results: The average value of L1-NB distance measurement was 7.18±2.31 mm and the L1-NB inclination measurement was 30.22˚±6.16˚. Conclusion: The average value of mandibular incisor position in students of SMAN 12 Banjarmasin was classified as protrusion, and the average inclination was also included in the proclination group.Keywords: banjar ethnic, cephalometry, lateral cephalometry, steiner analysis ABSTRAK Latar belakang: Penampilan menjadi salah satu hal terpenting bagi setiap orang di era modern ini. Ketidakharmonisan relasi gigi dan rahang atau yang dikenal dengan maloklusi akan menyebabkan penurunan penampilan seseorang.Posisi dan kemiringan gigi anterior ini harus menguntungkan untuk memastikan keharmonisan wajah yang maksimal. Usaha yang biasa dilakukan untuk mendapatkan keharmonisan wajah yang maksimal salah satunya melakukan perawatan ortodonti.Untuk mendukung perawatan tersebut, perlu dilakukan rontgen salah satunya teknik sefalometri dengan analisis Steiner.Tujuan: Penelitian ini bertujuan untuk mengetahui nilai pengukuran posisi insisif mandibula siswa SMAN 12 Banjarmasin yang berasal dari etnis Banjar ditinjau dari radiografi sefalometri lateral dengan menggunakan analisis Steiner. Metode: Pengukuran sefalometri dilakukan dengan menggunakan metode analisis Steiner pada landmark gigi; L1-NB. Analisis data dilakukan dengan metode statistik deskriptif. Sampel dalam penelitian ini merupakan data sekunder penelitian terdahulu dan mengambil siswa SMAN 12 Banjarmasin suku Banjar murni yang ditandai dengan minimal dua generasi (ayah, ibu, kakek, dan nenek) yang tidak pernah atau tidak sedang melakukan perawatan orthodonti dan telah bersedia menjadi subjek dalam penelitian. Hasil: Nilai rata-rata pengukuran jarak L1-NB sebesar 7.18±2.31 mm dan nilai pengukuran inklinasi L1-NB sebesar 30.22˚±6.16˚. Kesimpulan: Nilai rata-rata posisi insisif mandibula pada siswa SMAN 12 Banjarmasin tergolong protrusi, dan rata-rata inklinasi nya juga termasuk dalam golongan proklinasi. Kata kunci: analisis steiner, sefalometri, sefalometri lateral, suku banjar.

EFEKTIVITAS VIDEO ANIMASI DALAM PENURUNAN NILAI INDEKS PLAK Raket Rizki Rahmaningtyas; Widodo Widodo; Galuh Dwinta Sari; Diana Wibowo; Didit Aspriyanto
Dentin Vol 9, No 1 (2025)
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentin.v9i1.16560

Background: Dental plaque, as a microbial biofilm, is the main causative factor for caries. The 2018 Riskesdas survey showed that the proportion of dental and oral problems reached 57.6%, with damaged, cavitated, or painful teeth accounting for 45.3%. Only 2.8% of the population practiced correct tooth-brushing behavior, resulting in poor oral hygiene and plaque accumulation. Educational methods using animated videos are needed to improve tooth-brushing techniques in a more effective and easily understandable manner. Purpose: To analyze the effectiveness of animated videos in reducing plaque index values among students aged 7–9 years at SDN Marabahan 2. Methods: This study employed a quasi-experimental method with a pretest-posttest group design. The population consisted of students at SDN Marabahan 2, and 50 respondents were selected through simple random sampling. Results: The paired t-test revealed a significant decrease in plaque index values following the animated video education intervention, with a p-value of <0,001 (p<0,05). Conclusion: Animated videos are an effective and efficient health education medium for oral health promotion programs, demonstrating significant reduction in plaque index values. Keywords: Dental health education, animated videos, plaque index

UJI TOKSISITAS EKSTRAK DAUN KARAMUNTING (Rhodomyrtus tomentosa (Aiton) Hassk.) TERHADAP HATI TIKUS WISTAR Eka Dwita Natasya Fitri Siregar; Beta Widya Oktiani; Fajar Kusuma Dwi Kurniawan; Didit Aspriyanto; Ika Kusuma Wardani
Dentin Vol 8, No 2 (2024)
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentin.v8i2.13117

ABSTRACTBackground: Medicinal plants are types of plants that can ability to effectively treat illnesses. One such plant is caramunting leaves (Rhodomyrtus tomentosa (Aiton) Hassk). The substance in caramunting leaves includes an antibiotic that can counteract infections in the body. To determine the safe dosage of a drug, it is necessary to conduct toxicity tests in vivo on the liver of Wistar rats based on SGOT and SGPT levels. Objective: To analyze the toxic effects of administering caramunting leaf extract (Rhodomyrtus tomentosa (Aiton) Hassk.) in doses of 600 mg/kgBW, 1,200 mg/kgBW, and 2,400 mg/kgBW orally on rat liver based on SGOT and SPGT levels. Method: This study employed a true experimental posttest-only control group design to test the toxicity of caramunting leaf extract on SGOT and SGPT levels in the liver of Wistar rats given orally. Results: Following a 28-day experimental period on research animals, SGPT levels were observed to range from 34.9 to 218.1 U/L, while SGOT levels ranged from 56.1 to 201.9 U/L. These findings remain within the normal range, indicating that the extract does not have a toxic effect on SGPT and SGOT. Conclusion: Karamunting leaf extract at doses of 600 mg/kgBW, 1,200 mg/kgBW, and 2,400 mg/kgBW did not exhibit a toxic effect on SGPT and SGOT levels in Wistar rats.Keywords : Karamunting, Toxicity, SGOT, SGPT, Antibiotic. ABSTRAK Latar Belakang: Tanaman obat adalah tanaman yang mempunyai khasiat menyembuhkan suatu penyakit. Tanaman yang memiliki potensi tersebut adalah daun karamunting (Rhodomyrtus tomentosa (Aiton) Hassk). Kandungan yang terdapat pada daun karamunting bersifat sebagai antibiotik yang mampu mengatasi infeksi dalam tubuh. Untuk mengetahui batas rasional suatu obat, maka diperlukan penelitian uji toksisitas secara in vivo pada hati tikus wistar berdasarkan kadar SGOT dan SGPT. Tujuan: menganalisis efek toksik pemberian ekstrak daun karamunting (Rhodomyrtus tomentosa (Aiton) Hassk.) dosis 600 mg/kgBB, 1.200 mg/kgBB, dan 2.400 mg/kgBB per oral pada hati tikus berdasarkan kadar SGOT dan SPGT. Metode: Penelitian ini menggunakan design true eksperimental dengan desain posttest-only with control design untuk menguji toksisitas ekstrak daun karamunting terhadap kadar SGOT dan SGPT pada hati tikus wistar yang diberikan secara oral. Hasil: Setelah dilakukan percobaan pada hewan penelitian selama 28 hari didapatkan kadar SGPT 34,9-218,1 U/L dan kadar SGOT 56,1–201,9 U/L. Nilai tersebut menunjukkan hasil tidak melebihi rentang normal yang mengartikan bahan penelitian tidak memiliki efek toksik pada SGPT dan SGOT. Kesimpulan: Ekstrak daun karamunting dengan dosis 600 mg/kgBB, 1.200 mg/kgBB, dan 2.400 mg/kgBB tidak memiliki efek toksik pada kadar SGPT dan SGOT tikus Wistar.Tidak terdapat efek toksik dari pemberian ekstrak daun karamunting dosis 600 mg/kgBB, 1.200 mg/kgBB, dan 2.400 mg/kgBB secara per oral terhadap kadar SGOT dan SGPT tikus Wistar. Kata kunci : Antibiotik, Karamunting, SGOT, SGPT, Toksisitas

PERBEDAAN RELIABILITAS POLA SIDIK BIBIR DAN BENTUK PAPILLA INSISIF DALAM MENGIDENTIFIKASI JENIS KELAMIN PADA SUKU BANJAR Timothy Jogy Sotarduga Parhusip; Didit Aspriyanto; Isyana Erlita; Amy Nindia Carabelly; Rima Permata Sari
Dentin Vol 9, No 1 (2025)
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentin.v9i1.16563

Background: Indonesia is frequently affected by natural disasters, with incidents increasing from 2,574 in 2018 to 5,400 in 2023. Victim identification is a vital component of disaster response efforts. In forensic odontology, anatomical structures such as the incisive papilla and lip print patterns (cheiloscopy) serve as potential tools for identification. The incisive papilla offers advantages due to its morphological stability and protected position within the oral cavity, while lip prints are considered reliable due to their uniqueness and resistance to minor trauma. However, comparative studies assessing the accuracy of both methods in sex determination remain scarce and warrant further scientific investigation. Purpose: To analyze the difference in reliability between lip print patterns and incisive papilla in identifying sex among the Banjar ethnic group. Methods: This study employed a simple random sampling technique and utilized a non-paired categorical comparative analytic design. A cross-sectional approach was applied, involving observation and data collection conducted at a single point in time. Results: Based on Cohen’s Kappa coefficient, lip print pattern analysis demonstrated strong reliability (K = 0.839), whereas incisive papilla shape showed moderate reliability (K = 0.653). The Mann Whitney test identified statistically significant sex-based differences in lip print patterns within the Upper Right and Lower Middle quadrants, as well as notable morphological differences in incisive papilla shape between male and female subjects. Conclusion: Lip print patterns demonstrate higher reliability compared to the shape of the incisive papilla in determining sex among the Banjar ethnic population.Keywords: Forensic Odontology, Incisive Papilla, Lip Prints ABSTRAKLatar Belakang: Indonesia sering dilanda bencana alam, dengan 2.574 kejadian pada 2018 dan meningkat menjadi 5.400 pada 2023. Identifikasi korban menjadi langkah krusial, di mana metode identifikasi menggunakan papilla insisif dan pola sidik bibir dari rongga mulut dapat dimanfaatkan. Papilla insisif dinilai digunakan karena memiliki ciri stabil, terlindungi dalam rongga mulut, serta bentuk yang bervariasi berdasarkan jenis kelamin. Pola sidik bibir (cheiloscopy) juga unik dan tahan terhadap trauma minor, sehingga sering digunakan dalam odontologi forensik. Namun, studi mengenai perbandingan reliabilitas keduanya dalam penentuan jenis kelamin masih terbatas dan perlu diteliti lebih lanjut. Tujuan: Untuk menganalisis perbedaan reliabilitas antara pola sidik bibir dan papilla insisif dalam mengidentifikasi jenis kelamin pada suku Banjar. Metode: Penelitian ini menggunakan metode simple random sampling dan bersifat analitik komparatif kategorik tidak berpasangan. Pendekatan yang digunakan pada penelitian ini adalah pendekatan cross-sectional dengan mengamati, observasi, maupun pengumpulan data pada satu waktu yang sama. Hasil: Berdasarkan uji Cohen’s Kappa, reliabilitas pola sidik bibir menunjukkan kategori kuat dengan nilai K = 0,839, sedangkan bentuk papila insisif memiliki reliabilitas sedang dengan nilai K = 0,653. Uji Mann-Whitney menunjukkan adanya perbedaan yang signifikan antara jenis kelamin terhadap pola sidik bibir pada kuadran UR dan LM, serta terhadap bentuk papila insisif. Kesimpulan: Pola sidik bibir lebih reliabel dibandingkan bentuk papilla insisif dalam mengidentifikasi jenis kelamin pada suku Banjar.Kata Kunci: Odontologi Forensik, Papilla Insisif, Sidik Bibir

UJI EFEKTIVITAS ANTIBAKTERI EKSTRAK DAUN RAMANIA (Bouea Macrophylla Griff) TERHADAP PERTUMBUHAN BAKTERI Streptococcus mitis Lisa Shofa’ Nur Aini; Isyana Erlita; Didit Aspriyanto
Dentin Vol 8, No 1 (2024)
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentin.v8i1.12190

Background: The presence of cavities causes bacterial invasion and spread of infection to the pulp tissue. If left untreated, infection will lead to pulpal necrosis. Root canal treatment can prevent caries from developing into necrosis. The success of treatment is influenced by the preparation and irrigation stages. One of the bacteria that survived after the treatment was Streptococcus mitis. The irrigation solution used in treatment is a 2% chlorhexidine gluconate. Ethanol extract from Ramania leaves is known to contain flavonoids, phenols, alkaloids, steroids and terpenoids which proven to have antibacterial activity. Objective: To analyze the antibacterial effectiveness of Ramania leaf extract concentrations of 10%, 15%, 20%, 25% and 30% against the growth of Streptococcus mitis . Methods: The research conducted was a true experimental study with 6 treatment groups and 4 repetitions. This study used broth and agar dilution methods to determine the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of ramania leaf extract against Streptococcus mitis. Results: The MIC was shown by 10% concentration with a decrease in absorbance value of 1.726. The MBC in this study were not found because there was still bacterial growth in the concentrations of 10%, 15%, 20%, 25% and 30%. Conclusion: Ramania leaf extract concentrations of 10%, 15%, 20%, 25% and 30% can inhibit the growth of Streptococcus mitis with MIC value at a concentration of 10%. The MBC value was not found because the extract had not been able to kill the growth of Streptococcus mitis. Keywords : MBC, MIC, Ramania Leaf Extract, Streptococcus mitis

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