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STUDI ETNOFARFARMASI PADA SUKU ANAK DALAM (SAD) DI DESA PEMAYONGAN KECAMATAN SUMAY KABUPATEN TEBO PROVINSI JAMBI: STUDI ETNOFARFARMASI PADA SUKU ANAK DALAM (SAD) DI DESA PEMAYONGAN KECAMATAN SUMAY KABUPATEN TEBO PROVINSI JAMBI Perawati, Santi; Sutrisno, Deny; Ara Ofiana; Deny Sutrisno; Siti Hamidatul 'Aliyah; Lili Andriani; Rizky Yulion Putra; Rahmadevi; Desi Sagita; Yuni Andriani; Lia Anggresani; Barmi Hartesi
Jurnal Khazanah Intelektual Vol. 7 No. 2 (2023): Khazanah Intelektual
Publisher : Balitbangda Provinsi Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37250/khazanah.v7i2.198

Abstract

Background : Suku Anak Dalam is one of the tribes in Indonesia who still upholds their customs and culture, including their local knowledge about medicine using medicinal plants. One of the Suku Anak Dalam community lives in Pemayongan Village, Tebo Regency, Jambi Province. This ethnopharmaceutical research was conducted to find out various diseases experienced by Suku Anak Dalam and to know various natural resources that are used as treatment. Method : This type of research is a descriptive study using qualitative methods and sampling techniques, namely snowball sampling through open-ended interviews with informants. Results: The results of the study stated that diseases that was often experienced by the Suku Anak Dalam included fever, coughing, itching, shortness of breath and abdominal pain. Treatment of the disease by utilizing natural resources in the form of plants and animals. Plant parts used are roots, leaves, bark, and fruit sap, while for animals the parts used are fat, scales, and meat. The processing method is scraped, crushed, boiled, fried, while the use of these ingredients is eaten, drunk, and applied to the affected part. Conclusion : Based on the results of research that has been done there are 5 diseases that often occur and there are 9 kinds of plants and 5 animals that are used as traditional medicine. Keywords : Ethnopharmacy, Disease, Jambi, Suku Anak Dalam, Natural Resources
Isolasi dan Karakterisasi Bakteri Penghasil Enzim β-CGTase dari Tanah Perkebunan Ubi Jalar Kurniadi, Helman; Sagita, Desi; Hartesi, Barmi; Andriani, Lili; Dahlia, Dimbi
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 6 No 3 (2024): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2024.6.3.11779

Abstract

β-CGTase (β-siklodekstrin glikosil transferase) adalah enzim yang mengkonversi pati menjadi CD (siklodekstrin). CD adalah oligosakarida yang bernilai tinggi digunakan untuk modifikasi pangan, tekstil, kimia, obat-obatan, kosmetik hingga bioteknologi. Penelitian ini bertujuan untuk mendapatkan isolat bakteri penghasil β-CGTase dari tanah perkebunan ubi jalar. Skrining dilakukan menggunakan media Horikoshi yang mengandung indikator warna fenolftalein dan metil jingga, bakteri penghasil β-CGTase akan memberikan yellow hollow zone jika tumbuh pada media tersebut. Pengukuran aktifitas CGTase diukur menggunakan spektrofotometer UV-Vis. Didapatkan tiga isolat penghasil CGTase diberi kode BSP6A, BSP6B, dan BSP6C, yang memiliki karakteristik Gram positif, berbentuk basil, menghasilkan spora, bersifat motil, uji katalase positif, dan dapat memfermentasikan asam. Ketiga isolat ini mampu menghasilkan enzim CGTase optimum jika diinkubasi selama 18 dan 24 jam pada suhu 37°C dan pH 10, serta mampu mengubah pati menjadi CD jika diinkubasi selama 30 menit pada suhu 37°C dan pH 7.
Isolation of b-Cyclodextrin Gycosyl Transferase (b-CGTase) Producing Bacteria from Potato Plantation Soil Kurniadi, Helman; Sagita, Desi; Hartesi, Barmi
Biology, Medicine, & Natural Product Chemistry Vol 13, No 1 (2024)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2024.131.193-197

Abstract

Cyclodextrin glycosyltransferase (CGTase) is an extracellular enzyme produced by several microorganisms, particularly bacteria, that converts starch into cyclodextrin (CD). CD has various applications in fields such as food, textiles, pharmaceuticals, chemistry, and biotechnology. The objective of this study was to isolate b-CGTase-producing bacteria from soil in a potato plantation located in Kerinci, and to conduct their characterization. Screening was conducted using Horikoshi agar media with phenolphthalein and methyl orange color indicators. The results indicated that one isolate, coded CK-2, produced the optimum b-CGTase enzyme when incubated for 30 hours at 37 ºC and pH 10. The CGTase enzyme converted starch to CD when incubated for 60 minutes at 37 ºC and pH 7. The CK-2 isolate was identified as monobacilli Gram-positive bacteria, having spores, positive in the catalase test, and motile. The study concluded that the bacteria producing b-CGTase enzyme are found in the soil of potato plantations in Kerinci, which is believed to originate from the genus Bacillus sp.
Quantitative Analysis of Mineral in Seawater Concentrate from Pamekasan, Madura Using XRF and ICP-OES Methods Hartesi, Barmi; Wardhana, Yoga Windu; Muhaimin, Muhaimin; Sriwidodo, Sriwidodo; Khairinisa, Miski Aghnia; Dewi, Mayang Kusuma; Chaerunisaa, Anis Yohana
Journal of The Indonesian Society of Integrated Chemistry Vol. 16 No. 1 (2024): Journal of The Indonesian Society of Integrated Chemistry
Publisher : Pendidikan Kimia FKIP Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/jisic.v16i1.34656

Abstract

Seawater concentrate from Pamekasan is a product with high mineral content. The mineral content analysis of Pamekasan seawater concentrate was conducted using a comparative test of results from X-Ray Fluorescence Spectrometry (XRF) and Inductively Coupled Plasma Optical Emission Spectrometry (ICP-OES). This mineral content testing used a skin moisture device. The essential minerals found in Pamekasan seawater concentrate through analysis are Mg, Cl, K, Ca, Cr, Mn, Cu, and Zn. Statistical analysis results from XRF and ICP-OES on market products showed no significant difference in average results; however, there was a significant difference in average results in the samples. The most recommended method for analyzing seawater mineral concentrate from Pamekasan is ICP-OES due to its wider detection range and lower matrix interference.
KONSENTRASI HAMBAT MINUMUM ENZIM BROMELIN DARI KULIT DAN BONGGOL NANAS (Ananas comosus (L.) Merr) TERHADAP Staphylococcus aureus Sagita, Desi; Hartesi, Barmi; Fitri, Kurnia; Lufita, Lufita
Kartika : Jurnal Ilmiah Farmasi Vol 8 No 2 (2023)
Publisher : Fakultas Farmasi Universitas Jenderal Achmad Yani, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26874/kjif.v8i2.278

Abstract

Enzim bromelin adalah enzim proteolitik yang memiliki kemampuan menghidrolisis protein sehingga bisa berperan sebagai antibakteri. Enzim bromelin dapat diisolasi dari kulit, buah, bonggol Nanas (Ananas comosus (L.) Merr ). Tujuan penelitian ini adalah untuk mendapatkan konsentrasi enzim bromelin dari kulit dan bonggol buah Nanas dalam menghambat pertumbuhan Staphylococcus aureus. Bromelin diisolasi dengan cara ekstraksi dengan buffer pospat, dipurifikasi dengan ammonium sulfat 60%. Uji aktivitas antibakteri enzim bromelin dari kulit dan bonggol Nanas terhadap bakteri Staphylococcus aureus dilakukan secara invitro menggunakan difusi cakram dengan mengukur zona hambat.  Konsentrasi enzim bromelin yang digunakan adalah 1 %, 2%, 3% dan 4% dengan antibiotik kloramfenikol sebagai kontrol positif. Enzim bromelin dari kulit dan bonggol Nanas pada konsentrasi 4% menghambat pertumbuhan Staphylococcus aureus dengan zona hambat masing masing 19,23 mm dan 18,30 mm. Konsentrasi hambat minimum enzim bromelin dari kulit dan bonggol Nanas adalah 1% dengan zona hambat masing-masing 11,45 mm dan 12,24 mm. Enzim bromelin dari kulit dan bonggol Nanas memberikan aktivitas yang sama dalam menghambat pertumbuhan bakteri Staphylococcus aureus. Kata kunci : Bromelin; Kosentrasi Hambat Minumum; Presipitasi Amonium Sulfat.   ABSTRACT The Bromelains are proteolytic enzymes that have the ability to hydrolyze proteins so they can act as antibacterial. Bromelain enzymes can be isolated from the peel, fruit, and stem of pineapple (Ananas comosus (L.) Merr). The purpose of this study was to obtain the concentration of bromelain enzymes from the peel and stem of Pineapple in inhibiting the growth of Staphylococcus aureus. Bromelain was isolated by extraction with phosphate buffer, purified with 60% of ammonium sulfate. Antibacterial activity tests of bromelain enzymes from the peel and stem of pineapple against Staphylococcus aureus were carried out in vitro using disc diffusion by measuring clear zones. The bromelain enzyme concentration used was 1%, 2%, 3% and 4% with chloramphenicol antibiotics as a positive control. The results showed that bromelain enzymes from peel and stem of pineapple at 4% concentration inhibited the growth of Staphylococcus aureus with inhibition zones of 19.23 mm and 18.30 mm respectively. The minimum inhibitory concentration of bromelain enzymes from peel and stem of pineapple was 1% with inhibition zones of 11.45 mm and 12.24 mm respectively. Bromelain enzymes from the peel and stem of pineapple provide the same activity in inhibiting the growth of Staphylococcus aureus bacteria. Keywords : Bromelain; minimum inhibition concentration; ammonium sulfate precipitation
Formulasi Gel dari Fraksi Etil Asetat Batang Jarak Cina (Jatropha multifida L.) Efektivitas Terhadap Luka Bakar Giovana Zola, Elpa; Hartesi, Barmi; Anggresani, Lia
Journal of Pharmaceutical and Sciences JPS Volume 8 Nomor 3 (2025)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v8i3.966

Abstract

Background: Burns represent a condition of damaged or lost skin tissue due to high-temperature exposure. Oral therapy has limitations in penetrating hardened skin tissue, necessitating topical preparations such as gels that offer advantages in comfort, absorption capability, and ease of application. The stem of physic nut (Jatropha multifida L.) contains bioactive compounds including alkaloids, flavonoids, saponins, and tannins that may accelerate burn wound healing, particularly in its ethyl acetate fraction. Objective: This study aimed to formulate the ethyl acetate fraction of physic nut stem into a gel preparation and evaluate the effect of concentration variations on burn wound healing efficacy. Methods: Physic nut stems were extracted with 96% ethanol, then fractionated using n-hexane, ethyl acetate, and ethanol solvents. The ethyl acetate fraction was formulated into a hydrophilic gel at concentrations of 0.140%, 0.281%, and 0.421%. The gel preparations were evaluated through organoleptic tests, homogeneity, pH, spreadability, stability, irritation, and burn healing efficacy tests in rabbits. Results: Phytochemical screening revealed that the ethyl acetate fraction contained alkaloids, flavonoids, saponins, and tannins. All gel formulations met physical preparation requirements with skin-compatible pH (5.95-6.37). Formula F1 (0.140%) demonstrated optimal burn healing efficacy, equivalent to positive control (p > 0.05), and was topically safe (non-irritating). Conclusion: The ethyl acetate fraction of physic nut stem was successfully formulated into a stable and effective gel preparation for burn wound healing, with 0.140% as the optimal concentration. Further studies are required to examine toxicity and the mechanism of action of active compounds.
AKTIVITAS KOAGULAN DARI DAUN SEMBUNG RAMBAT (Mikania micrantha Kunth) SECARA IN VITRO Andriani, Lili; Perawati, Santi; Putri, Nadia; Hartesi, Barmi
Media Farmasi: Jurnal Ilmu Farmasi Vol. 17 No. 1: Maret 2020
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/mf.v17i1.16004

Abstract

Tumbuhan sembung rambat (Mikania micrantha Kunth) pada Suku Anak Dalam (SAD) Jambi digunakan untuk mengobati luka dan infeksi kulit yang bisa mengakibatkan perdarahan. Untuk menghindari perdarahan dibutuhkan senyawa yang kerjanya sebagai koagulan. Oleh karena itu pencarian senyawa koagulan penting dilakukan, salah satunya yang ada pada daun sembung rambat. Tujuan penelitian ini adalah untuk mengetahui aktivitas ekstrak dan fraksi daun sembung rambat sebagai koagulan secara in vitro. Metode penelitian yang digunakan yaitu ekstraksi daun sembung rambat menggunakan metode maserasi dengan pelarut etanol 70%, ekstrak etanol difraksinasi menggunakan metode ekstraksi cair-cair dengan pelarut n-heksan, etil asetat dan butanol. Aktivitas koagulan dilihat dengan cara melihat waktu pembekuan darah pada semua golongan darah (A, B, O dan AB) menggunakan metode Lee White dengan tiga konsentrasi ekstrak (10%, 20% dan 30%). Ekstrak daun sembung rambat pada semua konsentrasi menunjukkan aktivitas koagulan, dan fraksi etil asetat menunjukkan aktivitas koagulan yang lebih cepat untuk golongan darah O. Ekstrak dan fraksi daun sembung rambat memiliki aktivitas sebagai koagulan secara in vitro.
ANALISIS ASAM RETINOAT PADA KRIM PEMUTIH MALAM YANG BEREDAR DI KLINIK KECANTIKAN KOTA JAMBI PADA KECAMATAN JELUTUNG Hadriyati, Armini; Hartesi, Barmi; Fitri, Siska
Media Farmasi: Jurnal Ilmu Farmasi Vol. 17 No. 1: Maret 2020
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/mf.v17i1.16127

Abstract

Salah satu bahan pemutih berbahaya yang ditakutkan terdapat dalam krim adalah asam retinoat karena asam retinoat dapat menyebabkan kulit kering, rasa terbakar, teratogenik (cacat pada janin), dan menyebabkan kanker kulit. Tujuan penelitian ini untuk mengetahui apakah krim pemutih yang beredar di kecamatan Jelutung  kota Jambi mengandung asam retinoat serta untuk mengetahui berapa kadar asam retinoat yang terkandung dalam krim pemutih wajah tersebut. Metode yang digunakan untuk analisis asam retinoat pada krim pemutih menggunakan kromotografi cair kinerja tinggi fase terbalik. Hasil uji validasi menunjukkan nilai RSD 0,4887 %, parameter linearitas  r=0,9982, LOD 0,0468 ppm, LOQ 1,565 ppm. Hasil analisa kuantitatif kandungan asam retinoat pada krim 1 (0,032%), krim 2 (0,015%), krim 3 (0,014%) krim 4 (0,016%) dan krim 5 (0,011%). Berdasarkan persyaratan dari BPOM krim pemutih tidah boleh mengandung asam retinoat. Berdasarkan hasil penelitian dapat disimpulkan bahwa  krim pemutih wajah yang beredar di  kecamatan Jelutung kota Jambi mengandung asam retinoat.
Co-Authors Agusriani Agusriani Agusriani Agusriani, Agusriani Amelia Soyata Amelia Soyata Andini Ayodhia Fitri Anggi Rahmadani ani astuti, ani Anis Yohana Chaerunisaa, Anis Yohana Ara Ofiana Ardia Risuana Ardilla Novianti Armini Hadriyati armini hadriyati, armini Astridawati, Indah Bunga Debi Lestari Cindy Dwi Saputri Cindy Dwi Saputri Dahlia, Dimbi Desi Sagita Dewi, Rasmala Diah Merdekawati, Diah Dian Oktavia Dyan Putri Oktaviani Fhatia Medina Fitri, Kurnia Fitri, Siska Fitria Fitria Fitriya, Nina Giovana Zola, Elpa Haflin Haflin Haflin Haflin Haflin Haflin Haflin Haflin, Haflin Helsa Raudatul Qalbi Hesty Murtiah Ikhwansyah Ikhwansyah Indah Astridawati Indah Permata Sari Indah Permata Sari Indri Meirista Irna Dila Jelly Permatasari Jessyca Jovanica Jovanica, Jessyca Kartika Mustofa Khairinisa, Miski Aghnia Kurniadi, Helman Lara Syahila Lesra Indriani Lestari, Bunga Debi Lia Anggresani Lia Anggresani Lia Anggresani Lia Anggresani Lili Andriani Lili Andriani Lili Andriani Lili Andriani Lufita, Lufita M. Kurnia Pratama Mahadma Bhima Whinata MAYANG KUSUMA DEWI, MAYANG KUSUMA Medina, Fhatia Mesra, Novia Dita Muhaimin Muhaimin Muhammad Randa Nadia Monika Nadia Putri Nadia Putri Nina Fitriya Novia Dita Mesra Novia Dita Mesra Nurhuda Nurhuda Oktavia, Dian Oktaviani, Dyan Putri Okti Lestari Pitriani Pitriani, Rosa Pratama, M. Kurnia Puja Ariska Putri Dwi Ramadanti Rahmadani, Anggi Rahmadevi Ramadani, Suci Ramadanti, Putri Dwi Randa, Muhammad Risuana, Ardia Rizky Yulion Robiatul A’dawiyah SE Rosa Pitriani Rozalti Rozalti Ruri Putri Mariska Safitri, Wiwit Wulan Santi Perawati Santi Perawati Santi Perawati Santi Perawati, Santi Saputri, Cindy Dwi Sari, Suci Indah SE, Robiatul A’dawiyah Shafira Al Fadhilah Siska Fitri Siti Chairani Siti Hamidatul 'Aliyah Sriwidodo Sriwidodo, Sriwidodo Suci Indah Sari Suci Ramadani Suci Ramadani Susanti Susanti Sutrisno , Deny Swinche, Ulfa Deni Ulfa Deni Swinche Whinata, Mahadma Bhima Wiwit Wulan Safitri Wulandari, Konita Yoga Windu Wardhana, Yoga Windu Yuli Fitri Utami Yuliawati Yuliawati Yuni Andriani ‘Aliyah, Siti Hamidatul