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<![CDATA[Identifikasi Staphylococcus Aureus pada Fermentasi Ikan Pedo Menggunakan Teknik Pewarnaan Gram ]]>
<![CDATA[Afifatur Rizqiah]]>;
<![CDATA[Ardi Mustakim]]>
<![CDATA[ Jurnal Pendidikan Kimia, Fisika dan Biologi Vol. 2 No. 2 (2026): Maret : Jurnal Pendidikan Kimia, Fisika dan Biologi ]]>
Publisher : <![CDATA[Asosiasi Riset Ilmu Pendidikan Indonesia ]]>
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DOI: 10.61132/jupenkifb.v1i4.520
<![CDATA[This study aims to identify the presence of Staphylococcus aureus in fermented pedo fish (Decapterus spp.) products using the Gram staining technique. The Gram staining technique is a simple but effective method to distinguish Gram-positive and Gram-negative bacteria based on differences in their cell wall structure. Samples were taken from traditional fermented pedo fish and analyzed in a microbiology laboratory. The results showed the presence of Gram-positive coccus-shaped bacteria that clustered like grapes, in accordance with the morphological characteristics of S. aureus. This identification can be used as an initial step in evaluating the microbiological quality of fermented products.]]>
<![CDATA[Potensi Ekstrak Jahe (Zingiber officinale) sebagai Antibakteri Alami terhadap Kontaminasi Bakteri pada Petis Udang ]]>
<![CDATA[Suci Fabilla]]>;
<![CDATA[Ardi Mustakim]]>
<![CDATA[ Jurnal Cakrawala Pendidikan dan Biologi Vol. 2 No. 3 (2025): September: Jurnal Cakrawala Pendidikan dan Biologi ]]>
Publisher : <![CDATA[Asosiasi Riset Ilmu Pendidikan Indonesia ]]>
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DOI: 10.61132/jucapenbi.v2i3.526
<![CDATA[Shrimp petis is a traditional Indonesian fermented product that is susceptible to contamination by pathogenic bacteria such as Staphylococcus aureus, Escherichia coli, and Pseudomonas spp. Preservation is generally carried out with chemical antibiotics, but their use can cause side effects and microbial resistance. This study aims to examine the effectiveness of ginger extract (Zingiber officinale) as a natural antibiotic against bacteria in shrimp petis. Ginger extract is known to contain active compounds such as gingerol and shogaol which have antibacterial activity. Through literature studies and disc diffusion method analysis, it was found that ginger extract was able to produce an inhibition zone of 12–22 mm against test bacteria. These results indicate that ginger extract is effective in inhibiting the growth of pathogenic bacteria and has the potential to be used as a natural preservative. In addition, a combination of biological, chemical, and natural preservation approaches is recommended to maintain the quality and safety of shrimp petis. The use of ginger extract is in line with the trend of healthy and environmentally friendly food.]]>
<![CDATA[Uji Efektivitas Antimikroba Dettol, Betadine, dan Harpic terhadap Staphylococcus Aureus dengan Metode Difusi Cakram ]]>
<![CDATA[Citra Bunga Lestari]]>;
<![CDATA[Ardi Mustakim]]>
<![CDATA[ Jurnal Cakrawala Pendidikan dan Biologi Vol. 2 No. 3 (2025): September: Jurnal Cakrawala Pendidikan dan Biologi ]]>
Publisher : <![CDATA[Asosiasi Riset Ilmu Pendidikan Indonesia ]]>
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DOI: 10.61132/jucapenbi.v2i3.528
<![CDATA[This research was conducted to assess the antimicrobial activity of Dettol, Betadine, and Harpic against the bacterial growth of Staphylococcus aureus using the disk diffusion technique. The method involved placing sterile paper disks soaked with each test agent onto agar media previously inoculated with S. aureus. Observations revealed that Harpic formed the widest inhibition zone, measuring 15 mm, followed by Dettol with a 12 mm zone, and Betadine with the smallest at 8 mm. Meanwhile, the control disk exhibited no inhibitory effect (0 mm). These results suggest that Harpic has the highest antimicrobial potential among the three products tested in suppressing Staphylococcus aureus growth.]]>
<![CDATA[Uji Efektivitas Antibakteri Ekstrak Temulawak (Curcuma Xanthorrhiza) terhadap Sampel Tuak ]]>
<![CDATA[Dina Oktalia Putri]]>;
<![CDATA[Ardi Mustakim]]>
<![CDATA[ Algoritma : Jurnal Matematika, Ilmu pengetahuan Alam, Kebumian dan Angkasa Vol. 3 No. 4 (2025): Algoritma : Jurnal Matematika, Ilmu pengetahuan Alam, Kebumian dan Angkasa ]]>
Publisher : <![CDATA[Asosiasi Riset Ilmu Matematika dan Sains Indonesia ]]>
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DOI: 10.62383/algoritma.v3i4.639
<![CDATA[Palm wine (tuak) is a traditional fermented beverage that is prone to microbial contamination, thus requiring natural alternatives with antibacterial properties. This study aimed to determine the effectiveness of Curcuma xanthorrhiza (temulawak) extract in inhibiting microbial growth in tuak samples. The method used was the disc diffusion test with three extract concentrations, 25%, 50%, and 100%. The tuak samples were first femented, serially diluted, and inoculated on Nutrient Agar (NA). Sterile paper discs soaked in each extract were placed on the medium surface and incubated at room temperature for 24 hours. The results showed no visible inhibition zones around the discs at any concentration tested. The conclusion of this study is that temulawak extract at concentrations of 25–100% did not demonstrate antibacterial activity against microorganisms in tuak samples. This study serves as a foundational evaluation of natural antibacterial agents and highlights the need for further optimization of extraction techniques or targeted testing on specific bacterial isolates.]]>
<![CDATA[Pengaruh Ekstrak Orthosiphon Aristatus terhadap Pertumbuhan Mikroorganisme Uji dalam Praktikum Mikrobiologi Dasar ]]>
<![CDATA[Rezky Maulidiya]]>;
<![CDATA[Ardi Mustakim]]>
<![CDATA[ Algoritma : Jurnal Matematika, Ilmu pengetahuan Alam, Kebumian dan Angkasa Vol. 3 No. 4 (2025): Algoritma : Jurnal Matematika, Ilmu pengetahuan Alam, Kebumian dan Angkasa ]]>
Publisher : <![CDATA[Asosiasi Riset Ilmu Matematika dan Sains Indonesia ]]>
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DOI: 10.62383/algoritma.v3i4.643
<![CDATA[Orthosiphon aristatus is a medicinal plant that has the potential as a natural antibacterial agent. This study aims to evaluate the effect of cat's whiskers leaf extract on microbial growth using Nutrient Agar (NA) media with variations in extract concentrations of 100 gr, 50 gr, and 25 gr. Antibacterial activity tests were carried out using the well diffusion paper disk method, and observations of microbial growth inhibition zones were carried out after incubation for 24 hours at 37°C. The test results showed that there was inhibition of microbial growth that varied according to the concentration of the extract used; a concentration of 100 gr gave the largest inhibition zone compared to 50 gr and 25 gr. Data analysis showed that increasing extract concentration was directly proportional to increasing antibacterial effectiveness. These findings indicate that Orthosiphon aristatus leaf extract has the potential as a natural antibacterial that is effective in inhibiting microbial growth in NA media, so it has the potential to be developed as an active ingredient in pharmaceutical formulations.]]>
<![CDATA[Isolasi dan Identifikasi Bakteri E-Coli dari Tumbuhan Coleus Amboinicus ]]>
<![CDATA[Zinda Rizkia Aprilia]]>;
<![CDATA[Ardi Mustakim]]>
<![CDATA[ Nian Tana Sikka : Jurnal ilmiah Mahasiswa Vol. 3 No. 4 (2025): Nian Tana Sikka : Jurnal ilmiah Mahasiswa ]]>
Publisher : <![CDATA[Fakultas Ekonomi & Bisnis, Universitas Nusa Nipa ]]>
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DOI: 10.59603/niantanasikka.v3i4.940
<![CDATA[Escherichia coli (E. coli) is an indicator bacteria that is often used to assess the level of microbial contamination in food and plants. This study aims to isolate and identify the presence of E. coli from Coleus amboinicus plants which are known to have antibacterial activity. Fresh leaf samples of C. amboinicus were taken from the home yard environment. Isolation was carried out using the serial dilution method and planting on eosin methylene blue (EMB) placement media. Identification was carried out based on colony morphology, Gram staining, and biochemical tests including IMViC (Indole, Methyl Red, Voges-Proskauer, Citrate). The results showed the presence of metallic green colonies on EMB, which showed the characteristics of E. coli. Biochemical tests showed Indole (+), Methyl Red (+), Voges-Proskauer (-), and Citrate (-), which confirmed the isolate as E. coli. The presence of E. coli in C. amboinicus indicates the possibility of environmental fecal contamination, and is a concern in the processing and consumption of this medicinal plant.]]>
<![CDATA[Proses Pembuatan Media Agar dari Bahan Alami untuk Bakteri dan Jamur ]]>
<![CDATA[Siti Dwi Cahira]]>;
<![CDATA[Ardi Mustakim]]>
<![CDATA[ Nian Tana Sikka : Jurnal ilmiah Mahasiswa Vol. 3 No. 4 (2025): Nian Tana Sikka : Jurnal ilmiah Mahasiswa ]]>
Publisher : <![CDATA[Fakultas Ekonomi & Bisnis, Universitas Nusa Nipa ]]>
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DOI: 10.59603/niantanasikka.v3i4.944
<![CDATA[Agar is the main medium used in microbiology laboratories to grow bacteria and fungi because of its ability to form stable gels that are not easily degraded by microorganisms. However, the availability of expensive commercial agar and dependence on imports is a challenge for small-scale laboratories. This literature study aims to review the potential of natural materials such as local seaweed (Gracilaria sp.), banana peels, cassava starch, and coconut pulp as alternative media for microorganism growth. The results of the study indicate that Gracilaria has the ability to form gels similar to commercial agar, while banana peels and cassava starch are rich in nutrients but require the addition of thickeners. The main challenge lies in the sterilization process and media consistency. The use of these materials can be a cheap and sustainable solution in the development of microbiology media based on local resources.]]>
<![CDATA[Isolasi dan Identifikasi Bakteri serta Jamur pada Bekasam Ikan Mujair dengan Perlakuan Ekstrak Kunyit sebagai Agen Antimikroba ]]>
<![CDATA[Dini Anjani]]>;
<![CDATA[Ardi Mustakim]]>
<![CDATA[ Polygon : Jurnal Ilmu Komputer dan Ilmu Pengetahuan Alam Vol. 3 No. 4 (2025): Juli : Polygon : Jurnal Ilmu Komputer dan Ilmu Pengetahuan Alam ]]>
Publisher : <![CDATA[Asosiasi Riset Ilmu Matematika dan Sains Indonesia ]]>
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DOI: 10.62383/polygon.v3i4.640
<![CDATA[This study aims to isolate and identify microorganisms in the form of bacteria and fungi in tilapia fish waste and to demonstrate the effectiveness of turmeric extract (Curcuma longa Linn.) as an antimicrobial agent. Tilapia fish waste samples were fermented for seven days and then microorganisms were isolated using Nutrient Agar (NA) media for bacteria and Potato Dextrose Agar (PDA) for fungi. Turmeric extract treatment was carried out using the disc diffusion method. The results showed the presence of several species of Gram-positive and Gram-negative bacteria, as well as fungal colonies with dominant characteristics of Aspergillus sp. and Fusarium sp. Turmeric extract with a concentration of 20% showed a significant inhibition zone against the growth of pathogenic bacteria and fungi. The content of secondary metabolites such as alkaloids, tannins, and phenolics in turmeric extract is thought to be the main factor in antimicrobial activity.]]>
<![CDATA[Uji Aktivitas Antibakteri Ekstrak Jahe (Zingiber officinale) terhadap Mikroorganisme pada Tape Ketan Putih ]]>
<![CDATA[Shafitri Shafitri]]>;
<![CDATA[Ardi Mustakim]]>
<![CDATA[ Polygon : Jurnal Ilmu Komputer dan Ilmu Pengetahuan Alam Vol. 3 No. 4 (2025): Juli : Polygon : Jurnal Ilmu Komputer dan Ilmu Pengetahuan Alam ]]>
Publisher : <![CDATA[Asosiasi Riset Ilmu Matematika dan Sains Indonesia ]]>
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DOI: 10.62383/polygon.v3i4.641
<![CDATA[White glutinous rice fermentation (tape ketan putih) is a traditional food product containing various microorganisms, including Gram-positive bacteria that may act as contaminants and reduce product quality. Ginger (Zingiber officinale) contains active compounds such as gingerol and shogaol, known for their antibacterial properties. This study aimed to evaluate the antibacterial activity of ginger extract against microorganisms isolated from white fermented glutinous rice. Methods: Ginger extract was obtained by maceration using ethanol as a solvent and tested by disk diffusion method on Nutrient Agar against isolated microorganisms. Results: An inhibition zone was observed around the ginger extract disk, indicating moderate antibacterial activity against Gram-positive bacteria. Conclusion: Ginger (Zingiber officinale) extract shows potential as a natural antibacterial agent against Gram-positive bacteria in tape ketan putih and may be developed as a natural preservative for fermented products.]]>
<![CDATA[Pengujian Efektifitas Betadine, SoS, dan Hand Sanitizer, sebagai Antimikroba (Staphylococcus Aureus) ]]>
<![CDATA[Kayla Khairatifah Zalianti]]>;
<![CDATA[Ardi Mustakim]]>
<![CDATA[ Nian Tana Sikka : Jurnal ilmiah Mahasiswa Vol. 3 No. 4 (2025): Nian Tana Sikka : Jurnal ilmiah Mahasiswa ]]>
Publisher : <![CDATA[Fakultas Ekonomi & Bisnis, Universitas Nusa Nipa ]]>
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DOI: 10.59603/niantanasikka.v3i4.959
<![CDATA[Staphylococcus Aereus is a common us of skin and soft tissue infection. The aim of study was to evaluate the effectivaness of Betadine solution, Sos, and hand sanitizer as antimicrobial agent against S. Aereus. The study used a laboratory based experimental design, Where S. aereus was exposed to paper discs soaked in betadine, SOS, and hand sanitizer, the betadine disc showed a more significant antimicrobial effect than other This study shows that betadine is more effective than the others. Testing is carried out using the diffusion method where paper discs that have been soaked in antimicrobial substances are attached to the surface of agar media that has grown bacteria. In this way, a clear zone will be visible surrounding the disc paper as a sign of the microbe's sensitivity to the antimicrobial.]]>
