Articles
<![CDATA[THE INFLUENCE OF ORGANIC SOLVENT PROTEIN PRECIPITATION ON SDS PAGE PROTEIN PROFILE IN SERUM ]]>
<![CDATA[Tri Joko Raharjo]]>;
<![CDATA[Rusmiati Suprihatin]]>;
<![CDATA[Deni Pranowo]]>
<![CDATA[ Indonesian Journal of Chemistry Vol 7, No 3 (2007) ]]>
Publisher : <![CDATA[Universitas Gadjah Mada ]]>
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DOI: 10.22146/ijc.21680
<![CDATA[A study on the influence of organic solvent protein precipitation to the profile of the serum protein has been accomplished. The expected conditions were precipitation of abundant proteins present in serum result in increasing relative concentration of minor protein which can be useful for sample preparation for biomarker studies. The serum were precipitated with various diluted (<10%) acetonitrile, methanol and ethanol followed by SDS-PAGE analysis of the supernatant in order to investigate the protein profile. There were no linear relationship between solvent concentration and the number of protein bands. However, an optimal condition of precipitation was found which is by methanol 0.1%.]]>
<![CDATA[CALLUS INDUCTION AND PHYTOCHEMICAL CHARACTERIZATION OF Cannabis sativa CELL SUSPENSION CULTURES ]]>
<![CDATA[Tri Joko Raharjo]]>;
<![CDATA[Ogbuadike Eucharia]]>;
<![CDATA[Wen-Te Chang]]>;
<![CDATA[Robert Verpoorte]]>
<![CDATA[ Indonesian Journal of Chemistry Vol 6, No 1 (2006) ]]>
Publisher : <![CDATA[Universitas Gadjah Mada ]]>
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DOI: 10.22146/ijc.21776
<![CDATA[Callus of Cannabis sativa has been successfully induced from C. sativa explants and seedings. It seems that flowers are the best explant for callus induction and induction under light also give better results than induction in dark. Four cell culture lines were established from flower induced callus. Phytochemical profiles of C. sativa suspension cell cultures were investigated using HPLC and 1H-NMR. Cannabinoids and phenolic compounds related to cannabinoids such as flavonoids could not be found in the cell suspension cultures and there is no major chemical difference between the cell lines though they can visually be distinguished by their colors. Only in one cell line some aromatic compounds in the water/methanol extract could be observed in the 1H-NMR. Further investigations showed that none of these compounds are flavonoids. It seems that lack of cannabinoids in the cell cultures is related to lack of polyketide synthase activity.]]>
<![CDATA[SYNTHESIS OF 3,4-DIMETHOXY ISOAMYL CINNAMIC AS THE SUNSCREEN COMPOUND FROM CLOVE OIL AND FUSEL OIL ]]>
<![CDATA[Tutik Dwi Wahyuningsih]]>;
<![CDATA[Tri Joko Raharjo]]>;
<![CDATA[Iqmal Tahir]]>
<![CDATA[ Indonesian Journal of Chemistry Vol 2, No 1 (2002) ]]>
Publisher : <![CDATA[Universitas Gadjah Mada ]]>
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DOI: 10.22146/ijc.21933
<![CDATA[Synthesis of sunscreen compound 3,4-dimethoxy isoamyl cinnamic from clove oil and fusel oil has been done. The majoring component of clove oil that is eugenol has been isolated, followed with several chemical processes i.e. isomerization into isoeugenol, oxidation of the product to change into vanillin, then modification vanillin into veratraldehyde. From the fusel oil, we isolate isoamyl alcohol and modified by acetylation into isoamyl acetic. The final product could be produce by Claissen's condensation of isoamyl acetic and veratraldehyde to give 3,4-dimethoxy isoamyl cinnamic. All of the processes are followed with structure characterization using GC, IR, GC-MS and 1H-NMR spectroscopy. The result shown that compound has 46.98% purity and potent as the UV-B sunscreen's type (lmax = 313 nm). In-vitro sunscreen's activity of the compound was tested by UV-Vis spectrophotometry and resulting a maximum Sun Protection Factor value (SPFin-vitro) at low concentration, 10.25 mg/mL.]]>
<![CDATA[Validation of a Non-Specific Dye Real-Time PCR Assay for Porcine Adulteration in Meatball Using ND5 Primer ]]>
<![CDATA[Tri Joko Raharjo]]>;
<![CDATA[Ery Nourika Alfiraza]]>;
<![CDATA[Esti Enjelina]]>;
<![CDATA[Deni Pranowo]]>
<![CDATA[ Indonesian Journal of Chemistry Vol 17, No 2 (2017) ]]>
Publisher : <![CDATA[Universitas Gadjah Mada ]]>
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DOI: 10.22146/ijc.22646
<![CDATA[Porcine adulteration in meatball samples were analyzed using real-time polymerase chain reaction (RT-PCR), based on the ND5 primer obtained by previous study. This work consisted of three stages which were annealing temperature optimization, method validation, and application. DNA template was extracted using phenol-CIAA (chloroform-iso amyl alcohol) method. The optimum annealing temperature for ND5 primers (forward primer 5'-CATTCGCCTCACTCACATTAACC-3' and reverse primer 5'-AAGAGAGAGTTCTACGGTCTGTAG-3') was 58.0 °C, obtained after testing annealing at 50.5 to 59.5 °C gradient temperature with 5 °C interval. Melting curve analysis was done at 65.0 to 95.0 °C, with increasing temperature for 0.5 °C per 2 sec. Method was validated for its specificity, precision and limit of detection. RT-PCR method with ND5 primers produced 227 bp DNA fragment with 78.50 °C Tm value. From eight commercial meatball samples, one was detected containing porcine. The methods showed high specificity and precision, with experimentally determined limits for porcine were no less than 1%.]]>
<![CDATA[PCR Primer Specific CaMV 35S Promoter to Detect Transgenic Soybean in Indonesia Commercial Soy Bean and Tempeh ]]>
<![CDATA[Tri Joko Raharjo]]>;
<![CDATA[Surajiman Surajiman]]>
<![CDATA[ Indonesian Journal of Chemistry Vol 17, No 3 (2017) ]]>
Publisher : <![CDATA[Universitas Gadjah Mada ]]>
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DOI: 10.22146/ijc.24163
<![CDATA[In the framework of the supervision and enforcement of the regulation regarding the content of soybean transgenic in food and processed foods such as tempeh, a reliable testing method is indispensable. Performance specific primer PCR amplification with promoter of CaMV 35S tested to detect the presence of GMOs. The parameters tested were specificity, precision and cut off detection using CRM transgenic soybean. The method is reliable to detect transgenic soybean specifically and has the annealing temperature at 59 °C during the 30 cycle standard PCR condition. The method did not show any false positive and false negative results meaning good precision. The cut off the methods is up to 2 copies total DNA of soybean or less than 104 copies of the CaMV 35S promoter. Observation to the commercial soybeans and tempeh found that most of the commercially available soybean in Indonesia are transgenic (8 of 10 sample) while all tested tempeh sample were detected have been fermented from transgenic soybeans.]]>
<![CDATA[Forgery Detection Beef with Mice Meat (Mus musculus) in Meatballs Using Real-Time Polymerase Chain Reaction (Real-Time PCR) Primer Specific for a Target Mitochondrial DNA ND-1 Gene ]]>
<![CDATA[Tri Joko Raharjo]]>;
<![CDATA[Gilang Aji Pratama]]>;
<![CDATA[Irma Nuryanti]]>;
<![CDATA[Rarastoeti Pratiwi]]>
<![CDATA[ Indonesian Journal of Chemistry Vol 19, No 1 (2019) ]]>
Publisher : <![CDATA[Universitas Gadjah Mada ]]>
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DOI: 10.22146/ijc.27542
<![CDATA[The expensive beef have encouraged counterfeiting beef on processed food products such as meatballs. Mice meat is frequently reported used for adulteration of beef. The accurate method is needed to ensure the supervision of food safety. This study reports the use of DNA testing to detect the presence of mice meat in meatballs with real-time PCR primer specific. PCR primers designed based on the ND-1 gene of mice mitochondrial DNA with the sequence are 5’-CGGCATCCTACAACCATTTGC-3’ and 5’-CGGCTCGTAAAGCTCCGAA-3’, respectively, target 294 bp DNA fragment. The real-time PCR can specifically detect the presence of the mice meat in a meatball with no detection the presence of beef, mutton, chicken, pork, and horsemeat. The method showed good precision shown by the CV of repeatability test at 2%, much lower than the requirement of < 25%. Real-time PCR was able to deliver positive results for as low as 0.5 ng DNA template, equivalent to 0.08 copies of genome DNA of mice equal to 80–150 copies of mtDNA. By using standard phenol-chloroform DNA isolation technique, this method is able to detect contamination of mice meat in meatball up to 1%. Three commercial meatballs confirmed contaminated by mice meat using the method.]]>
<![CDATA[Anticancer Activity of Venom Protein Hydrolysis Fraction of Equatorial Spitting Cobra (Naja sumatrana) ]]>
<![CDATA[Naseer Ahmed]]>;
<![CDATA[Garnis Putri Erlista]]>;
<![CDATA[Tri Joko Raharjo]]>;
<![CDATA[Respati Tri Swasono]]>;
<![CDATA[Slamet Raharjo]]>
<![CDATA[ Indonesian Journal of Chemistry Vol 23, No 2 (2023) ]]>
Publisher : <![CDATA[Universitas Gadjah Mada ]]>
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DOI: 10.22146/ijc.80720
<![CDATA[Bioactive peptides play an important role in targeting cancer cells. Venom protein from Naja sumatrana can be explored as a source of bioactive peptides. This research aims to identify and study the molecular docking of bioactive peptides (BPs) from trypsin hydrolysate of N. sumatrana venom protein which was fractionated using an SPE C18 column. The venom of N. sumatrana was hydrolyzed with trypsin enzyme. The protein hydrolysate was then fractionated using an RP-SPE HyperSep Retain PEP column, and the peptide fractions were tested for their anticancer activity against MCF-7 breast cancer cells using the (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method. Identification of peptides in the active fraction was carried out through high-resolution mass spectrometry. The identified peptides were molecularly docked with the EGFR receptor using AutoDock Vina. The results showed that the degree of hydrolysis was 74.7%. The 75% methanol fraction is the active fraction against MCF-7 cells, with an IC50 value of 4.80 μg/mL and a selectivity index of 5.00. Peptide-active anticancer fractions with the sequence of NSLLVK, SSLLVK and TVPVKR were successfully identified and exhibited high binding affinity values, good RMSD values, and the most suitable model for the epidermal growth factor receptor.]]>
<![CDATA[The Essential Factors and Trend Development of IoT Smart Box ]]>
<![CDATA[Surjandy]]>;
<![CDATA[Meyliana]]>;
<![CDATA[Condrobimo, Andreas Raharto]]>;
<![CDATA[Eka Widjaja, Henry Antonius]]>;
<![CDATA[Atmadja, Wiedjaja]]>;
<![CDATA[Susanto, Rudy]]>;
<![CDATA[Sablan, Bruno]]>
<![CDATA[ Indonesian Journal of Information Systems Vol. 6 No. 1 (2023): August 2023 ]]>
Publisher : <![CDATA[Program Studi Sistem Informasi Universitas Atma Jaya Yogyakarta ]]>
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DOI: 10.24002/ijis.v6i1.6794
<![CDATA[Boxes for drug storage are handy and essential and play a role in helping the healing process and support maintaining patient health regularly for a long time. SmartBox is not a new thing, but because of its importance, it, SmartBox continues to be developed to date according to the needs needed. In previous research, the formation of SmartBox was only for particular things. This study tries to unify the 17 papers obtained with the help of a publish and perish application which is then carried out a literature review and using the VosViewer application to get an overview of trend development and the essential factors or aspects in the formation of SmartBox and its development. In addition to getting the trend of SmartBox development, this research also finds seven essential factors that will be useful for Smartbox development. With this research, it hopes that it will facilitate the development of SmartBox in the future.]]>
<![CDATA[Optimalisasi Kinerja Kondensor Sistem Refrigerant Plant di Kapal MV. Dian Cordelia ]]>
<![CDATA[Andika, Rio]]>;
<![CDATA[Nugraha, Markus Asta Patma]]>;
<![CDATA[Hasnur, Juliandri]]>;
<![CDATA[Susanto, Rudy]]>
<![CDATA[ Jurnal Cakrawala Bahari Vol. 7 No. 1 (2024): Jurnal Cakrawala Bahari ]]>
Publisher : <![CDATA[Politeknik Pelayaran Sumatera Barat ]]>
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DOI: 10.70031/jkb.v7i1.43
<![CDATA[Sistem pendingin yang menerima uap pendingin tekanan tinggi yang panas dari kompresor dan mengenyahkan panas pengembunan itu dengan cara mendinginkan uap pendingin tekanan tinggi yang panas ke titik embunnya dengan cara mengenyahkan panas sensibelnya. Metode yang digunakan pada penelitian ini yaitu kualitatif. Jenis metode kualitatif yang digunakan yaitu deskriptif kualitatif yang berarti mendeskripsikan dan menggambarkan fenomena-fenomena yang ada, baik bersifat alamiah maupun rekayasa manusia, yang lebih memperhatikan mengenai karakteristik, kualitas, keterkaitan antar kegiatan, yang dilakukan melalui observasi, wawancara, dan dokumentasi. Hasil penelitian disimpulkan bahwa kondensor dalam keadaan kotor akan mengganggu proses kondensasi gas freon menjadi cair. Freon yang dikondensasikan tidak dapat mencukupi kebutuhan pendinginan pada ruang pendingin, sehingga suhu ruang pendingin tidak dapat tercapai karena suatu kebocoran pada daerah tekanan rendah akan menyebabkan udara masuk kedalam sistem freon.]]>
<![CDATA[SISHANKAMRATA: An Approach to a Versatile Asymmetric Strategy for Weak and Strong from Clausewitz Trinity Perspective ]]>
<![CDATA[Maulidiyawan, Robby]]>;
<![CDATA[Susanto, Rudy]]>;
<![CDATA[Suhirwan]]>
<![CDATA[ Jurnal Internasional Bisnis, Humaniora, Pendidikan dan Ilmu Sosial Vol 6 No 2 (2024): International Journal of Business, Humanities, Education and Social Sciences ]]>
Publisher : <![CDATA[Universitas Teknologi Yogyakarta ]]>
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DOI: 10.46923/ijbhes.v6i2.469
<![CDATA[This study aims to examine the relevance of this system at a time when asymmetric war elements dominate many current conflicts. This research has two problem formulations, the research objectives of which are as follows: (1) How does SISHANKAMRATA integrate the principles of Clausewitz's Trinity to create a cohesive and adaptive defence strategy against modern asymmetric threats? (2) What are the strengths and limitations of SISHANKAMRATA as a strategic model for weak and strong actors? The method used is a qualitative research method of library research with the acquisition of secondary data such as laws, doctrine, posture, books and scientific articles. This study concludes that SIHANKAMRATA is versatile for facing conventional, asymmetric, and even hybrid threats. The nature of SISHANKAMRATA also invites people to prioritize this system through quantity. However, the weakness lies in the quality, in terms of the preparation stage, and in the later stage of maintaining it. It is crucial during the conflict since Indonesia possesses vast territory and archipelago.]]>
