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Histological Analysis of Somatic Embryogenesis from Immature Zygotic Embryo of Wild Banana Musa acuminata ssp. malaccensis Handayani, Tri; Martanti, Diyah; Prawestri, Apriliana Dyah; Tihurua, Eka Fatmawati; Maharijaya, Awang; Wahyu, Yudiwanti; Sobir; Witjaksono
Journal of Tropical Crop Science Vol. 11 No. 03 (2024): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.11.03.307-319

Abstract

Somatic embryogenesis, a crucial plant regeneration method, has become indispensable for crop improvement, particularly for species reliant on somatic cell manipulation techniques. Optimization of this process necessitates an understanding of the developmental stages involved. This study investigates the histological aspects of somatic embryogenesis in Musa acuminata ssp. malaccensis derived from immature zygotic embryos. Through detailed histological analysis, we aimed to elucidate the morphological changes and cellular organization occurring during the various stages of somatic embryogenesis, from induction, culture proliferation, and somatic embryo development to plantlet conversion. The initial stages of embryogenesis, characterized by nodules, were primarily composed of meristematic cells with high cell division activity. These cells contained tetrad-like structures that could develop into distinct two- and four-celled proembryoids or proembryogenic aggregates. Our histo-anatomical analysis revealed that embryogenic cultures proliferated through multiple pathways simultaneously: somatic embryo budding, proembryo formation, and pro-embryonic mass formation from both internal and peripheral cells. At the stage of somatic embryo development, embryos with a well-defined protoderm layer, containing cells with prominent nuclei and dense cytoplasm, potentially regenerate into plantlets. Furthermore, histological examination revealed the presence of procambium within mature somatic embryos, which subsequently developed into the vascular system of the complete plantlet
An Efficient Somatic Embryogenesis and Plant Regeneration from Immature Embryo of Wild Banana Musa acuminata ssp. malaccensis Handayani, Tri; Martanti, Diyah; Prawestri, Apriliana Dyah; Maharijaya, Awang; Wahyu, Yudiwanti; Sobir; Witjaksono
Journal of Tropical Crop Science Vol. 12 No. 01 (2025): Journal of Tropical Crop Science
Publisher : Department of Agronomy and Horticulture, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jtcs.12.01.172-184

Abstract

Wild banana Musa acuminata ssp. malaccensis, an ancestor of cultivated bananas, possesses valuable genetic diversity, including resistance genes to fusarium wilt, and demonstrates high environmental adaptability. These traits are important for pre-breeding programs, whether by conventional breeding, which is a lengthy process, or by taking advantage of somatic cell manipulation techniques such as somatic hybridization, which requires an efficient plant regeneration system like somatic embryogenesis. We have established an efficient and comprehensive protocol for somatic embryogenesis of this wild Musa using immature zygotic embryo explants covering culture induction and proliferation, somatic embryo development, and subsequent plant conversion. Embryogenic culture was induced on a complex-modified MS medium supplemented with 1 mg.L-1 2,4-D auxin or 5 mg.L-1 picloram. The embryogenic cultures proliferated in the form of granular or nodular structures, which was best obtained by reducing the picloram concentration to 1 mg.L-1 and combining it with the same concentration of 2,4-D at a half-strength macro-nutrient of basal medium. Embryo development from embryogenic cultures and regeneration into shoots. Proembryos as granular structures spontaneously matured into early-stage somatic embryos upon withdrawal of the strong auxin inducer. Increasing the sucrose and gelling agent concentrations in the growth medium improved somatic embryo formation from embryogenic cultures. The frequency of shoot formation from developed somatic embryos was increased by incorporating 0.5 mg.L-1 BA and 0.5 mg.L-1 GA3 into the regeneration medium.
Understanding gene interactions controlling resistance to pepper yellow leaf curl disease through phenotype-based analysis Sayekti, Tri Wahono Dyah Ayu; Syukur, Muhamad; Hidayat, Sri Hendrastuti; Sobir, Sobir; Maharijaya, Awang
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 53 No. 2 (2025): Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24831/jai.v53i2.65965

Abstract

Pepper yellow leaf curl disease (PYLCD) is the primary disease that affects chili plants, resulting in a loss of quality and quantity. The identification of chili plants resistant to PYLCD is a solution to support optimal chili cultivation. The purpose of this study was to estimate genetic parameters to obtain information on the genetic control of chili resistance to PYLCD. The genotypes used in this study consisted of four parental, four hybrid genotypes, and 180 individuals of the F2 population. The results showed that the genotypes "F6074" and "IPBC12" were categorized as resistant, while the genotypes “IPBC5” and “YUNI” were categorized as susceptible. The distribution of resistance level in chili peppers to yellow curly leaf disease tends to follow a ratio of 13:3, with a dominance of susceptible traits. The assumption for this phenomenon is that resistance to PYLCD is controlled by at least two genes. Both genes have dominant and recessive epistatic interactions. Keywords: Begomovirus; chi-square; epistasis; resilience
Pengurangan Pupuk Anorganik oleh Pupuk Kandang Kambing terhadap Kualitas Umbi dan Ketahanan Hama pada Bawang Merah Romdoni, Arif; Maharijaya, Awang; Siti Yuliani, Titiek
Journal of Comprehensive Science Vol. 3 No. 5 (2024): Journal of Comprehensive Science (JCS)
Publisher : Green Publisher Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59188/jcs.v3i5.712

Abstract

Penggunaan pupuk anorganik perlu dikurangi karena dapat meninggalkan residu kimia pada tanah serta menurunkan kualitas bawang merah. Pupuk organik kotoran kambing (POKK) dapat dijadikan alternatif untuk mengurangi penggunaan pupuk anorganik tersebut. Penelitian dilakukan pada Maret hingga Juni 2019 di lahan sentra bawang merah Desa Sukorejo, Kabupaten Nganjuk, Jawa Timur. Penelitian bertujuan untuk menentukan pupuk anorganik yang dapat dikurangi oleh POKK terhadap kualitas umbi bawang merah dan ketahanannya terhadap hama. Penelitian menggunakan rancangan petak terpisah dengan rancangan lingkungan kelompok lengkap teracak. Aplikasi 10 ton ha-1 POKK dalam budidaya bawang merah di Kabupaten Nganjuk dapat mengurangi penggunaan pupuk anorganik hingga 75%. Penambahan 10 ton ha-1 POKK dan 25 kg N; 6.26 kg P2O5; 31.31 kg K2O ha-1 mampu menghasilkan persentase diameter umbi besar (> 2 cm) sebanyak 75.18%. Tingkat kejadian dan serangan Spodoptera exigua dapat diturunkan menjadi 0% pada minggu ke 3 dengan kombinasi 50.09 kg N; 12.52 kg P2O5; 62.52 kg K2O ha-1 dan 10 ton ha-1 POKK. Aplikasi 10 ton ha-1 POKK dapat menurunkan tingkat kejadian hama Liriomyza chinensis pada minggu ke 6 menjadi 34.50%.
Respon Delapan Genotipe Melon (Cucumis melo L.) terhadap Perlakuan KNO3 Huda, Amalia Nurul; Suwarno, Willy Bayuardi; Maharijaya, Awang
Jurnal Hortikultura Indonesia (JHI) Vol. 9 No. 2 (2018): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (324.337 KB) | DOI: 10.29244/jhi.9.2.84-92

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ABSTRACTPotassium (K) is a mineral nutrient needed by crops that influences the quality of fruits and vegetables. The objective of this study was to elucidate the interaction effects of genotype by KNO3 treatment (G × KNO3) on fruit traits of melon. The experiment was conducted from August to October 2016 at Tajur II Experimental Station of IPB, Bogor, following a split plot design with three replicates. The main plot was two levels of KNO3 and the subplot was eight genotypes, consisting of seven test genotypes and one check variety (Eagle). The KNO3 treatments were applied weekly, started from 7-49 DAP with 5 g L-1 concentration, ±200 ml plant-1. G × KNO3 interaction effects were significant for sugar content and titratable acidity (TA). P34 had relatively high sugar content and TA. P25 showed a significant increase of sugar content when KNO3 is added, while Eagle, P311, and P34 showed significant decrease of sugar content. Days to male flowering, days to hermaphrodite flowering, and fruit weight had high broad sense heritability (repeatability), while days to harvest, fruit diameter, flesh thickness, rind thickness, and sugar content had moderate heritability. Fruit weight had significant positive correlation with fruit diameter, flesh thickness, and rind thickness. Application of KNO3 fertilizer in practical is therefore suggested for the postive-response genotypes to KNO3.Key words: fruit quality, KNO3, melon, sugar contentABSTRAKKalium (K) merupakan mineral yang dibutuhkan oleh tanaman yang berpengaruh terhadap kualitas buah dan sayur. Pada budidaya melon umumnya, sumber mineral K diperoleh dalam bentuk KNO3. Penelitian ini bertujuan mengidentifikasi pengaruh interaksi genotipe × perlakuan KNO3 (G × KNO3) terhadap peningkatan kualitas buah melon. Percobaan dilakukan pada bulan Agustus sampai Oktober 2016 di Kebun Percobaan IPB Tajur II, Bogor menggunakan rancangan petak terbagi (split plot design) dengan tiga ulangan. Petak utama merupakan perlakuan KNO3 dengan dua taraf, dan anak petak merupakan genotipe dengan delapan taraf, yang terdiri dari tujuh genotipe uji dan satu varietas pembanding (Eagle). Perlakuan KNO3 dilakukan setiap minggu mulai 7–49 HST dengan konsentrasi 5 g L-1 dan volume aplikasi ± 200 ml tanaman-1. Interaksi G × KNO3 berpengaruh nyata terhadap karakter kadar gula dan total asam tertitrasi (TAT). Genotipe P34 memiliki kadar gula dan TAT yang relatif tinggi. P25 merupakan genotipe yang menunjukkan respon positif berupa peningkatan kadar gula pada perlakuan KNO3, namun sebaliknya genotipe Eagle, P311, dan P34 justru menunjukkan penurunan kadar gula yang signifikan pada perlakuan KNO3. Karakter yang memiliki nilai heritabilitas (repeatabilitas) arti luas yang tergolong tinggi adalah umur berbunga jantan, umur berbunga hermaprodit, dan bobot buah, sedangkan yang heritabilitasnya tergolong sedang adalah umur panen, diameter buah, tebal daging buah, tebal kulit buah, dan kadar gula. Bobot buah berkorelasi positif dengan diameter buah, tebal daging buah, dan tebal kulit buah. Aplikasi pemupukan KNO3 secara praktis disarankan pada genotipe melon yang memiliki respon positif terhadap KNO3.Kata kunci: kandungan gula, KNO3, kualitas buah, melon
The characteristic Performace of Quantitative Flowering Characters and Metabolomic Profile of Shallot (Allium cepa var. aggregatum) Induced by Vernalization Maharijaya, Awang; Purwito, Agus; Marlin; Sobir
Jurnal Hortikultura Indonesia (JHI) Vol. 9 No. 3 (2018): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (307.116 KB) | DOI: 10.29244/jhi.9.3.197-205

Abstract

Flowering initiation is regulated by the internal and external condition of plant. Vernalization is considered to induce flower initiation on shallots (Allium cepa var. aggregatum). This research objective was to analyze the flowering quantitatif characters and metabolomic profile of shallot during vernalization on bulb development. Vernalization was carried out at 8 0C for 6 weeks treatment were four bulb growth stage i.e non-vernalized bulbs (S0), vernalization on embryonic stage (S1), vernalized bulbs on 1 cm of shoot stage (S2) and vernalized bulbs on 2 cm of shoot stage (S3). Vernalization treatment in early stage increased the number of tillers, number of umbel, diameter of umbel and percentage of flowering compared to another stage. The early stage of bulbs growth was the effective stage in receiving vernalization treatment. The bigger number and diameter of umbel lead to the higher percentage of flowering in shallot plant. The number and diameter of umbel can be used as character of selection for the percentage flowering character in shallot. Metabolomic analysis has identified of 104 specific metabolites from different vernalization treatments and clustered shallot into three groups. The early stadium of bulbs development (embryo stadia and stadia 1 cm buds) contains specific metabolomes (phytol and 2-propanone) as the indicator of reproductive phase. Keywords: correlation, flower induction, hierarchical cluster analysis, metabolomic
Performance and Poduction of G2 Potato Tuber Seeds Using Diference Seed Sources Azhari, Andi; Maharijaya, Awang; Sobir
Jurnal Hortikultura Indonesia (JHI) Vol. 10 No. 1 (2019): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (252.237 KB) | DOI: 10.29244/jhi.10.1.27-35

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Produksi benih G2 kentang (Solanum tuberosum L.) menggunakan umbi dan setek dari dua varietas lokal dilakukan untuk mengetahui keragaan dan produksinya di Lapangan. Saat ini di Indonesia, setek kentang digunakan secara terbatas untuk produksi umbi G0 dan G1 pada kondisi terkontrol (rumah kaca atau rumah kasa). Rancangan Kelompok Lengkap Teracak (RKLT) faktorial digunakan pada penelitian ini dengan varietas (RGH01 dan Medians) dan sumber benih (umbi (G0) dan setek) sebagai faktor dan lima ulangan. Data dianalisis dengan sidik ragam yang kemudian dilanjutkan dengan uji Duncan. Tanaman kentang yang ditanam dengan sumber benih setek menghasilkan jumlah daun dan cabang yang lebih banyak pada kedua varietas. Namun, tidak berbeda nyata pada peubah tinggi tanaman. Jumlah umbi per tanaman menunjukkan hasil tidak berbeda nyata pada seluruh kombinasi perlakuan dengan 5.2 hingga 5.9 umbi per tanaman. Produktivitas umbi yang lebih besar dihasilkan oleh tanaman kentang dengan sumber benih umbi pada kedua varietas. Persentase umbi kelas S (<40 g) yang lebih besar diperoleh dari tanaman kentang dengan sumber benih setek sebesar 82.0% (Medians) dan 94.1% (RGH01). Kata kunci: perbanyakan benih, perbanyakan kentang, setek, Solanum tuberosum L., umbi
Heritability, Correlation, and Path Analysis on Various Characters of Tomato (Solanum lycopersicum) under Shading and Normal Condition Ritonga, Arya Widura; Chozin, Muhamad Achmad; Syukur, Muhamad; Maharijaya, Awang; Sobir
Jurnal Hortikultura Indonesia (JHI) Vol. 10 No. 2 (2019): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (383.399 KB) | DOI: 10.29244/jhi.10.2.85-93

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Character selection information is essential for plant breeding program. SSH3 tomato genotype (shade-loving genotype), 4974 (shade-sensitive genotype), and F2 population derived from “SSH3 x 4974” were evaluated to estimate the heritability, correlation, direct and indirect effects between yield and various yield atributting characters in tomato under shading and normal condition at Pasir Kuda Station, Bogor Agriculture University, West Java, Indonesia from July until October 2016. The results showed that plant height, leaf length, leaf width, leaf weight, specific leaf weight, fruit weight, fruit number per plant and fruit weight per plant had high heritability under normal condition, while plant height, fruit set, fruit number of plant and fruit weight per plant had high heritability under shading condition. High direct positive effect on yield per plant was resulted from fruit weight and fruit number of plant under shading and normal condition, while leaf width had direct positive effect on fruit weighr per plant only under shading condition. High indirect positive effect on yield per plant was resulted from fruit set, fruit length and fruit diameter under shading and normal condition, while plant heigh had high indirect effect on yield per plant only under shading condition. Fruit set, fruit weight, and fruit number per plant characters were potential to be used as character selection for tomato productivity in normal conditions. Plant height, fruit set and fruit number per plant were potential to be used as character selection for tomato productivity under shading condition. Keywords: direct effect, fruit set, indirect effect, low light tolerant
Comparative analysis of Genetic Diversity among Pod Vegetables Genetic Resources Potential in Indonesia Revealed by ISSR Markers Rif’atunidaudina, Ria; Sobir; Maharijaya, Awang
Jurnal Hortikultura Indonesia (JHI) Vol. 10 No. 3 (2019): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (597.977 KB) | DOI: 10.29244/jhi.10.3.161-172

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Kacang tunggak (Vigna unguiculata ssp unguiculata), kacang panjang (V. unguiculata ssp sesquipedalis), kacang Bogor (V. subterranea), koro kratok (Phaseolus lunatus), kacang merah (P. vulgaris), koro pedang (Canavalia ensiformis), koro benguk (Mucuna pruriens) dan kecipir(Psophocarpus tetragonolobus) merupakan sayuran polong penting di Indonesia dari tanaman kacang-kacangan. Tanaman tersebut bernilai ekonomi tinggi dan kaya nutrisi dengan kandungan protein biji keringnya yang hampir sama dengan kedelai dan dapat menunjang kesehatan manusia sekaligus menunjang pangan di masa depan. Informasi keragaman genetik di antara sayuran polong yang berbeda masih terbatas. Penelitian ini bertujuan untuk menganalisis keragaman genetik dalam dan antar sayuran polong yang berbeda spesies berdasarkan marka ISSR. Sebanyak 32 aksesi sayuran polong dianalisis menggunakan 11 primer ISSR menghasilkan 80 pita polimorfik dengan persentase polimorfisme sebesar 100% dan primer informatif yang didapat adalah PKBT 3 dan PKBT 6. Hasil analisis kluster dan analisis PCA mengelompokkan 32 aksesi tersebut ke dalam delapan kluster dan menunjukkan mayoritas aksesi yang satu spesies mengelompok bersama. Koefisien kemiripan genetik Gower keseluruhan aksesi berkisar antara 0.425-0.988, dan koefisien kemiripan delapan spesies berkisar antara0.444 – 0.700. Hubungan kekerabatan antara spesies V. subterranean - C. Ensiformis memiliki jarak genetik terkecil, sedangkan jarak genetik terbesar ditemukan antara spesies P. vulgaris - M. pruriens. Penentuan kekerabatan tersebut bermanfaat untuk pemahaman yang lebih lanjut tentang hubungan di antara sayuran polong yang berbeda spesies, yang umumnya dianggap sebagai kelompok yang kompleks dengan variabilitas fenotipik yang tinggi. Kata kunci: jarak genetik, kacang-kacangan, koefisien kemiripan, pengelompokan, polimorfisme
Peningkatan Pertumbuhan dan Pengendalian Rebah Kecambah Bibit Cabai Menggunakan Teknologi Agens Hayati pada Berbagai Ketinggian Tempat Suryo Wiyono; Awang Maharijaya; Tamrin Khamidi; Ahmad Fauzi Ridwan; Kusuma Darma
Jurnal Hortikultura Indonesia (JHI) Vol. 12 No. 2 (2021): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jhi.12.2.138-146

Abstract

Pengunaan agens hayati dalam perlakuan benih cabai merupakan alternatif pengendalian penyakit tular tanah berupa penyakit rebah kecambah yang disebabkan oleh berbagai cendawan pathogen tanah. Penelitian ini bertujuan (1) mengkaji keefektivan agens hayati dalam perkecambahan dan pertumbuhan bibit cabai besar dan (2) dan pengaruhnya terhadap pengendalian penyakit damping off di persemaian cabai pada berbagai ketinggian tempat. Penelitian ini terdiri dari tiga lokasi percobaan, (1) ketinggian tempat satu/KT I (947 m dpl) pada bulan Juni-September 2019 di lahan petani Desa Bumijawa, Kecamatan Bumijawa, Kabupaten Tegal, (2) ketinggian tempat dua/KT II (61 m dpl) pada bulan Juni-September 2019 di Desa Jembayat Kecamatan Margasari Kabupaten Tegal, dan (3) ketinggian tempat tiga/KT III (891 m dpl) pada bulan September-Desember 2020 di Desa Tuwel Kecamatan Bojong Kabupaten Tegal. Penelitian ini menggunakan rancangan acak kelompok satu faktor yaitu teknik pembibitan berupa konvensional dan teknologi agens hayati. Hasil penelitian menunjukkan aplikasi teknologi agens hayati terbukti secara nyata efektif meningkatkan perkecambahan (indeks vigor) dan pertumbuhan bibit cabai. Teknologi agens hayati mampu mengendalikan penyakit damping off pada kemunculan pertama penyakit dan kejadian penyakit di dataran rendah (KT II). Teknologi agens hayati direkomendasikan dalam teknik pembibitan cabai.
Co-Authors ,, Rosminah Abdul Hakim Ady Daryanto Afaf, Sausanil Agus Purwito Ahmad Fauzi Ridwan Alveno, Vitho Amalia Nurul Huda Amarilis, Shandra Amrullah, Rizki Abi Anas Dinurrohman Susila Arum Sholikhah Azhari, Andi Bagas Akmala Putra Bambang Sapta Purwoko Bonjok Istiaji Danu Kuncoro Dhiya Nabilla Ardhani Diny Dinarti Diyah Martanti, Diyah Dwinita Wikan Utami Eka Fatmawati Tihurua Endang Gunawan Eny Widajati Erna Siaga, Erna Erna Sinaga Erviana Eka Pratiwi Fazat Fairuzia Hariyadi Harti, Heri Hidayat, dan Purnama Ika Roostika Inayah Yasmin Kamila Iswari Saraswati Dewi Iswari Saraswati Dewi Ita Aprilia J. K. J. Laisina Juharni Kalsum Yulifar, Andi Sri Ummi Ketty Suketi Kristianto Nugroho Kunio Yamada Kusmana Kusuma Darma Kusuma Darma Lina Herlina Lina Herlina M A Chozin Marlin Masaki Ochiai Megayani Sri Rahayu Muhamad Syukur Muhammad Mahmud Muhammad Ridha Alfarabi Istiqlal Neng Neni Neni, Neng Ni Made Armini Wiendi Ni Made Armini Wiendi, Ni Made Niken Kendarini Nina Agusti Widaningsih Nursalma, Linda Prawestri, Apriliana Dyah Prima Muklisa PURNAMA HIDAYAT Purwoko, Bambang Sapta Purwoko Qadir, Abdul Rahmawati, Rika Sri Ramdhani, Cahyati Reflinur Reflinur Reflinur Reflinur Rerenstradika T. Terryana Ria Rif’atunidaudina Riti, Estriana Ritonga , Arya Widura Ritonga, Arya W Ritonga, Arya Widura Rizki Abi Amrullah Romdoni, Arif Rosminah, Rosminah Salma, Linda Nur Sani, Mutiyara Sari Mahyendra Sayekti, Tri Wahono Dyah Ayu Siti Yuliani, Titiek Sobir Sobir Sobir Sobir Sobir Sobir Sobir Sobir SRI HENDRASTUTI HIDAYAT Suhartanto , M. Rahmad Sukma, Dewi Sulassih, . Suryo Wiyono Suwarto Suwarto Syarifah Iis Aisyah Tamrin Khamidi Tamrin Khamidi Tengku Laila Kamaliah Tri Handayani Ulil Azmi Nurlaili Afifah Usep Jenudin Willy Bayuardi Suwarno Witjaksono Yudiwanti Wahyu E. Kusumo Yuliani, Titiek