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Genetic Diversity Analysis Using Resistance Gene Analog-Based Markers to Support Morphological Characterization of Shallots Lina Herlina; Reflinur Reflinur; Kristianto Nugroho; Rerenstradika T. Terryana; Sobir Sobir; Awang Maharijaya; Suryo Wiyono
Jurnal AgroBiogen Vol 14, No 2 (2018): December
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v14n2.2018.p65-74

Abstract

Shallot (Allium cepa var. aggregatum) is one of the most important vegetable crops grown in Indonesia. The limited knowledge available on the genetic diversity and the threat of plant disease have been major problems to maintain high shallot production in Indonesia. Development of molecular markers linked to disease resistance is required for molecular breeding activity in this crop. This study aimed to assess the genetic diversity at conserved domain of resistance gene analog (RGA) in a set of 36 Indonesian shallot genotypes to complement morphological characterization. Twelve morphological and fifteen molecular markers traits were investigated in an attempt to characterize and to discriminate the Indonesian shallots genotypes. Characterization at orphological level indicated that phenotypic variance was highest for total bulb weight (TWB, cv = 99.39%) and the least for the plant height (PH, cv = 28.16%). The correlation analysis between traits showed that TWB and number of bulb (NB), TWB and bulb weight per plant (WB), NB and WB, and WB and PH were positively correlated. Molecular analysis revealed a total of 1,512 alleles with an average of 1.946 alleles per locus. The Polymorphism Information Content (PIC) values ranged from 0.253 to 0.676 and six out of 15 RGA markers were highly informative with PIC values ≥0.50. Based on cluster analysis, the 36 Indonesian shallot genotypes were clearly discriminated into six major groups. These results revealed that the RGA-based markers could support the morphological characterization in evaluating the genetic diversity of shallots. 
Longday Photoperiod Accelerates Flowering in Indonesian Non-Flowering Shallot Variety Fazat Fairuzia; Sobir Sobir; Awang Maharijaya; Masaki Ochiai; Kunio Yamada
AGRIVITA, Journal of Agricultural Science Vol 44, No 2 (2022)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v44i2.3053

Abstract

The shallot flowering ability is essential to increase productivity by assembling superior varieties through the hybridization and botanical seed or true shallot seed (TSS). The photoperiod length greatly influences flowering in shallot, which plays a significant role in the flowering initiation. This research aims to study flowering responses with different photoperiods in shallots. Six shallot varieties: Lokananta, Bima Brebes, Rubaru, Palasa, Biru Lancor, and Batu Ijo, were grown in a greenhouse under a short-day photoperiod, 10 hours of natural condition photoperiod for 60 days. Furthermore, half of the plants are moved to long-day treatment. The temperature during plantation is around from 7-13°C (similar to Indonesia’s upland temperature). The results indicate that long-day photoperiod increased the shallot flowering response on the five shallot varieties tested, except Rubaru. Rubaru could not produce flowers under both photoperiod treatments 120 days after plantation (DAP). Lokananta variety is the most responsive variety to flower, even on short-day photoperiod. Palasa variety, which could not flower under Indonesian photoperiod conditions, could produce flowers on long-day treatment and underplanting conditions at low to moderate temperatures.
TINGKAT SERANGAN LALAT BUAH DAN THRIPS PADA CABAI DENGAN BERBAGAI TEKNIK PENGENDALIAN HAMA DAN PENYAKIT Tamrin Khamidi; Suryo Wiyono; Kusuma Darma; Awang Maharijaya
JURNAL BIOINDUSTRI (JOURNAL OF BIOINDUSTRY) JURNAL BIOINDUSTRI: VOL. 3 NO. 2 (2021)
Publisher : Universitas Trilogi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31326/jbio.v3i2.755

Abstract

Teknologi mikroba intensif merupakan paket integrasi berbagai agens hayati dalam budidaya cabai yang diketahui efektif dalam menekan berbagai penyakit cabai.  Pengaruh teknik tersebut terhadap dua hama utama cabai yaitu lalat buah (Bactrocera sp) dan thrips (Thrips sp) belum diketahui. Tujuan penelitian ini adalah untuk meneliti pengaruh teknik mikroba intensif terhadap tingkat serangan lalat buah dan thrips pada cabai.  Penelitian terdiri dari empat perlakuan yaitu mikroba intensif penuh, mikroba intensif di pesemaian, konvensional dan tanpa pengendalian.  Penelitian dilakukan di tiga lokasi dengan ketinggian tempat yang berbeda. Pengamatan tingkat serangan lalat buah dilakukan pada umur 10 dan 11 minggu setelah tanam, sementara pengamatan terhadap kerusakan thrips dilakukan seminggu sekali mulai 2 hingga 10 minggu setelah tanam.  Perlakuan mikroba intensif secara nyata mengurangi tingkat serangan lalat buah.  Perlakuan mikroba intensif mengurangi serangan thrips pada dua lokasi tanam yaitu Margasari dan Bojong, namun tidak berpengaruh nyata pada satu lokasi lainnya yaitu Bumijawa.
Evaluation of Chili Nazla IPB as An Ornamental Chilli for Releasing Variety Abdul Hakim; Muhamad Syukur; Sobir; Awang Maharijaya; Syarifah Iis Aisyah; Dewi Sukma; Arya Widura Ritonga; Muhammad Ridha Alfarabi Istiqlal; Sulassih; Bagas Akmala Putra; Arum Sholikhah
Jurnal Biologi Tropis Vol. 23 No. 3 (2023): July - September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i3.4976

Abstract

Chili (Capsicum annuum L.) exhibits a wide range of variations in terms of crown shape, fruit shape, fruit color, leaf color, leaf shape, and taste. While chili is commonly known as a food plant, it also serves as an attractive ornamental plant. Specifically, ornamental chili plants are desired to possess specific traits, such as compact growth, high fruit yield, and a variety of fruit colors within a single plant. This study aims to comprehensively describe the quantitative and qualitative superiority of the Nazla IPB variety in terms of its characteristics. The research was conducted in the Leuwikopo Darmaga experimental garden's greenhouse, located in Bogor, utilizing a randomized complete block design with four replications. The Nazla IPB variety, which is an open-pollinated (OP) cultivar, stands out due to its short plant height, ranging from 17.83 to 26.42 cm, making it highly suitable for ornamental purposes. Furthermore, the chili plants of the Nazla IPB variety possess a compact crown shape, enhancing their visual appeal, coupled with their short stature (ranging from 21.83 to 26.69 cm). The primary distinguishing features of the ornamental chili variety Nazla IPB include: 1) the color of the leaves, which transition from green (Green 137 C) to purple (Purple N186 A); 2) the black color of the young chili fruit (Black 203 A); 3) the fruit shape of Nazla IPB, which can be described as moderately triangular; and 4) the fruit undergoes a color transformation from black to dark green and finally to red. Nazla IPB has potential to serve as an attractive decorative plant option.
Diallel Analysis of Chili Pepper Resistance to Melon Aphid (Aphis gossypii Glover) Infestation in Seedling Phase Ady Daryanto; Muhamad Syukur; Awang Maharijaya; Purnama Hidayat
Agrotech Journal Vol 3, No 2 (2018): Agrotech Journal (ATJ)
Publisher : Universitas Sembilanbelas November Kolaka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31327/atj.v3i2.868

Abstract

Aphis gossypii Glover is one of important insect pest in Indonesia. Genetic analysis of resistance to A. gossypii is required in plant breeding program to obtain host-plant resistance cultivar. Diallel analysis was used to estimate genetic parameters for chili pepper resistance to A. gossypii infestation in early generation. The objective of this research was to estimate genetic parameters of chili pepper resistance to A. gossypii infestation with diallel crossing design. The F1 and parent plants were arranged in randomized competed block design with three replication. Resistance lines was measured using choice test laboratory screening techniques. Two aphids were infested per plant and stopped 12 days after first infestation. Different lines respond was detected as shown by significant numbers of aphid per leaf, total aphid per plant, and total winged aphid per plant. There were no maternal effect and resistance were controlled by recessive and polygenic genes. Gene effects for resistance to aphid’s infestation were additive and dominance. Dominance effect larger than additive effects. Broad-sense heritability values were high but narrow-sense heritability values were very low
Evaluasi Teknis dan Manajerial Kegiatan Pemupukan Kelapa Sawit di Kabupaten Landak, Kalimantan Barat Sari Mahyendra; Hariyadi; Awang Maharijaya
Buletin Agrohorti Vol. 11 No. 2 (2023): Buletin Agrohorti
Publisher : Departemen Agronomi dan Hortikultura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agrob.v11i2.47135

Abstract

Aktivitas pemeliharaan tanaman kelapa sawit merupakan upaya yang sangat penting dalam meningkatkan produktivitas yang optimal. Salah satu upaya dalam meningkatkan produktivitas yaitu dengan pemupukan. Pemupukan merupakan proses penambahan unsur hara dan perbaikan struktur tanah serta penggantian unsur-unsur hara yang hilang. Pemupukan harus sesuai dengan dosis yang telah ditentukan dalam buku rekomendasi pemupukan dan tepat waktu pemberiannya. Penelitian bertujuan mengevaluasi kegiatan pemupukan tanaman kelapa sawit berdasarkan efektivitas pemupukan. Kegiatan dilaksanakan pada Februari hingga Mei 2019 di Kabupaten Landak, Kalimantan Barat. Metode yang dilaksanakan selama kegiatan penelitian yaitu metode langsung dan metode tidak langsung. Percobaan disusun menggunakan uji t berpasangan terhadap pemupukan berbagai jenis pupuk yang berbeda pada kelapa sawit. Pengamatan dilakukan terhadap keefektifitasan pemupukan meliputi kaidah 6T (tepat jenis, tepat dosis, tepat waktu, tepat cara, tepat tempat, dan tepat alat), prestasi kerja dan gejala defisiensi unsur hara. Hasil pengamatan menunjukkan gejala defisiensi unsur hara secara visual menunjukkan tanaman kelapa sawit masih mengalami kekurangan unsur Magnesium (Mg), Kalium (K), Boron (B), dan Nitrogen (N). Kata kunci: defisiensi, efektivitas, rekomendasi, unsur hara
The Sensitivity of Four Garlic Genotypes on Low Temperatures and the Role on Dormancy Breaking Niken Kendarini; Syarifah Iis Aisyah; Awang Maharijaya; Sobir Sobir
AGRIVITA Journal of Agricultural Science Vol 45, No 3 (2023)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v45i3.4177

Abstract

The garlic seed bulbs cannot be planted immediately after harvest because has dormant period. Bulb dormancy can be broken by exposure of pre-planting bulbs to low temperatures. The research aimed to determine the sensitivity of different bolting types of garlic genotypes at low temperatures and the role of low temperatures on dormancy break. The experiment was conducted from March to June 2019 at the Universitas Brawijaya, using a nested design with three replications. Chinese hardneck and softneck, Sangga Sembalun and Tawangmangu Baru were the garlic accessions used in the study. The storage temperature treatments at 3 and 7°C; and room temperature at 27°C. Chinese softneck bulb had the highest sprouting and rooting after 3 and 7°C storage and was sensitive to low temperatures. The Chinese hardneck had the lowest sprouting and was highly insensitive to cold stress. Sangga Sembalun and Tawangmangu Baru had sprouted bulbs in between these Chinese genotypes, and they were insensitive and highly insensitive to low temperatures, respectively. Metabolites of 5-hydroxymethyl-2-furancarboxaldehyde, palmitic acid, diallyl trisulfide, and 2,3-dihydro-3,5-dihydroxy-6-methyl-4(H)-pyran-4-one played important roles in the bulb response to low temperature stress and directly or indirectly involved in the sprouting and rooting in dormant garlic cloves.
Genetic Diversity and Population Structure Analysis of Potato Somaclones Nina Agusti Widaningsih; Ika Roostika; Dwinita Wikan Utami; Awang Maharijaya; Kusmana; Usep Jenudin; Sobir
HAYATI Journal of Biosciences Vol. 30 No. 6 (2023): November 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.6.1008-1016

Abstract

Cultivated potato varieties are tetraploid and vegetatively propagated. Therefore genetic improvement for new traits is less effective through hybridization. The mutation is considered an alternative method for crop improvement of those varieties. This study aimed to evaluate the effects of gamma-ray irradiation on increasing genetic diversity among the somaclones and the changes in population structure. Forty-four somaclones were used as observed materials. The population was grown in a screen house using a completely randomized design with genotype (somaclone) as a single factor. Thirteen qualitatively and quantitatively morphological characters were observed. Six SSR markers were used for analyzing the population structure. The selection process was based on a weighting method divided into quartiles. Selected numbers were taken from quartile 3. Morphological analysis of stems and leaves resulted in five different somaclones, with significant differences in anthocyanin appearance and intensity of the green color of the leaflets. Diversity criteria based on quantitative characteristics showed a high level of diversity with a high heritability for tuber weight, length, and diameter and a moderate heritability for tuber number. Analyzing the population structure has offered insight into how gamma irradiation affected the somaclones. As a result of the selection, 12 somaclones met the requirements to serve as seed sources for field testing. It is expected that the results of this study will provide information about the diversity caused by gamma-ray irradiation treatment on potato somaclones and a method for improving the efficiency of the initial selection of potato populations.
Etiology of Anthracnose Disease on Shallots Caused by Colletotrichum gloeosporioides Amrullah, Rizki Abi; Wiyono, Suryo; Maharijaya, Awang; Purwito, Agus
Jurnal Fitopatologi Indonesia Vol 19 No 5 (2023): September 2023
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.19.5.206-214

Abstract

Penyakit antraknosa dapat terjadi pada seluruh fase pertumbuhan tanaman bawang merah (Allium cepa var. aggregatum) dan belum diketahui proses perkembangannya serta karakteristik penyebabnya. Penelitian dilakukan untuk mempelajari etiologi penyakit antraknosa yang meliputi perkembangan gejala, periode inkubasi dan karakteristik patogen melalui pengujian secara in vivo dan in planta. Awal gejala ialah munculnya bercak putih yang kemudian membesar hingga menyebabkan jaringan mati. Gejala khas yang ditimbulkan ialah adanya pola konsentris berwarna jingga kecokelatan pada bercak dan memiliki periode inkubasi 2-4 hari. Karakteristik morfologi koloni patogen berwarna putih, konidium berbentuk silindris, hifa bersekat. Karakter lain ditandai dengan keberadaan aservulus dan setae. Patogen yang diidentifikasi secara molekuler menunjukkan 99.51% homologi dengan Colletotrichum gloeosporioides aksesi KT390192.1 dari China yang berasal dari tanaman tembakau.
TINGKAT SERANGAN LALAT BUAH DAN THRIPS PADA CABAI DENGAN BERBAGAI TEKNIK PENGENDALIAN HAMA DAN PENYAKIT Tamrin Khamidi; Suryo Wiyono; Kusuma Darma; Awang Maharijaya
JURNAL BIOINDUSTRI (JOURNAL OF BIOINDUSTRY) JURNAL BIOINDUSTRI: VOL. 3 NO. 2 (2021)
Publisher : Universitas Trilogi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31326/jbio.v3i2.755

Abstract

Teknologi mikroba intensif merupakan paket integrasi berbagai agens hayati dalam budidaya cabai yang diketahui efektif dalam menekan berbagai penyakit cabai.  Pengaruh teknik tersebut terhadap dua hama utama cabai yaitu lalat buah (Bactrocera sp) dan thrips (Thrips sp) belum diketahui. Tujuan penelitian ini adalah untuk meneliti pengaruh teknik mikroba intensif terhadap tingkat serangan lalat buah dan thrips pada cabai.  Penelitian terdiri dari empat perlakuan yaitu mikroba intensif penuh, mikroba intensif di pesemaian, konvensional dan tanpa pengendalian.  Penelitian dilakukan di tiga lokasi dengan ketinggian tempat yang berbeda. Pengamatan tingkat serangan lalat buah dilakukan pada umur 10 dan 11 minggu setelah tanam, sementara pengamatan terhadap kerusakan thrips dilakukan seminggu sekali mulai 2 hingga 10 minggu setelah tanam.  Perlakuan mikroba intensif secara nyata mengurangi tingkat serangan lalat buah.  Perlakuan mikroba intensif mengurangi serangan thrips pada dua lokasi tanam yaitu Margasari dan Bojong, namun tidak berpengaruh nyata pada satu lokasi lainnya yaitu Bumijawa.
Co-Authors ,, Rosminah Abdul Hakim Ady Daryanto Afaf, Sausanil Agus Purwito Ahmad Fauzi Ridwan Alveno, Vitho Amalia Nurul Huda Amarilis, Shandra Amrullah, Rizki Abi Anas Dinurrohman Susila Arum Sholikhah Azhari, Andi Bagas Akmala Putra Bambang Sapta Purwoko Bonjok Istiaji Danu Kuncoro Dhiya Nabilla Ardhani Diny Dinarti Diyah Martanti, Diyah Dwinita Wikan Utami Eka Fatmawati Tihurua Endang Gunawan Eny Widajati Erna Siaga, Erna Erna Sinaga Erviana Eka Pratiwi Fazat Fairuzia Hariyadi Harti, Heri Hidayat, dan Purnama Ika Roostika Inayah Yasmin Kamila Iswari Saraswati Dewi Iswari Saraswati Dewi Ita Aprilia J. K. J. Laisina Juharni Kalsum Yulifar, Andi Sri Ummi Ketty Suketi Kristianto Nugroho Kunio Yamada Kusmana Kusuma Darma Kusuma Darma Lina Herlina Lina Herlina M A Chozin Marlin Masaki Ochiai Megayani Sri Rahayu Muhamad Syukur Muhammad Mahmud Muhammad Ridha Alfarabi Istiqlal Neng Neni Neni, Neng Ni Made Armini Wiendi Ni Made Armini Wiendi, Ni Made Niken Kendarini Nina Agusti Widaningsih Nursalma, Linda Prawestri, Apriliana Dyah Prima Muklisa PURNAMA HIDAYAT Purwoko, Bambang Sapta Purwoko Qadir, Abdul Rahmawati, Rika Sri Ramdhani, Cahyati Reflinur Reflinur Reflinur Reflinur Rerenstradika T. Terryana Ria Rif’atunidaudina Riti, Estriana Ritonga , Arya Widura Ritonga, Arya W Ritonga, Arya Widura Rizki Abi Amrullah Romdoni, Arif Rosminah, Rosminah Salma, Linda Nur Sani, Mutiyara Sari Mahyendra Sayekti, Tri Wahono Dyah Ayu Siti Yuliani, Titiek Sobir Sobir Sobir Sobir Sobir Sobir Sobir Sobir SRI HENDRASTUTI HIDAYAT Suhartanto , M. Rahmad Sukma, Dewi Sulassih, . Suryo Wiyono Suwarto Suwarto Syarifah Iis Aisyah Tamrin Khamidi Tamrin Khamidi Tengku Laila Kamaliah Tri Handayani Ulil Azmi Nurlaili Afifah Usep Jenudin Willy Bayuardi Suwarno Witjaksono Yudiwanti Wahyu E. Kusumo Yuliani, Titiek