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Determination of anthracnose (Colletotrichum gloeosporioides) resistance group in shallot (Allium cepa var. aggregatum) Rizki Abi Amrullah; Maharijaya, Awang; Purwito, Agus; Wiyono, Suryo
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 51 No. 3 (2023): Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24831/jai.v51i3.47429

Abstract

Shallot anthracnose, caused by Colletotrichum gloeosporioides, is a devastating disease in a tropical country with high humidity and rainfall. Chemical control of anthracnose is neither economical nor eco-friendly, and genetic resistance is considered an efficient management method. This study aimed to determine the resistance groups of several shallot varieties and predict resistance heritability characteristics. In this study, a total of 13 Indonesian shallot varieties were evaluated for anthracnose resistance and separated into two groups, resistance and susceptible, based on K-means clustering developed by using disease resistance/susceptibility characteristics such as incubation period, disease incidence, disease severity, and spot diameter. The results indicate that the Agrihorti, Maja Cipanas, Batu Ijo, and Rubaru varieties were identified as resistant groups based on lower disease severity and incidence, smaller spot diameter, and longer incubation period. Maja Cipanas and Rubaru were more consistent in all variables, which is recommended as a source of genetic resistance genotypes. On the other hand, the Biru Lancor, Bima Brebes, Srikayang, Violetta, Slupu Merah, Pancasona, Sakato, Katumi, and Kuning varieties were identified as susceptible groups based on high disease severity and incidence, large spot diameter, and short incubation period. Keywords: susceptibility characteristics, genetic resistance, k-means clustering, disease severity
Callus formation response from immature male flower explant of plantain banana (Musa acuminata x Musa balbisiana cv. Kepok) treated by 2,4-D and BAP Dhiya Nabilla Ardhani; Maharijaya, Awang; Megayani Sri Rahayu
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 52 No. 1 (2024): Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24831/jai.v52i1.49008

Abstract

Callus induction is an important step in indirect somatic embryogenesis. The aim of this study was to obtain an optimal medium for the callus formation of plantain bananas (cv. Kepok). The research was conducted from May to August 2022 at the Tissue Culture Laboratory of PT. ITCI Kartika Utama, Penajam Paser Utara District, East Kalimantan. The explant used an immature male flower of plantain banana (cv. Kepok). The experiment used a completely randomized design with two factors concentration of 2,4-D (1,2 and 4 ppm) and BAP (5, 10, and 15 ppm). The appearance of callus was monitored daily until it developed explants. On the final observation day, the percentage of explants with callus, as well as the color and texture of the callus, were assessed. The research findings suggest that the optimal medium for inducing callus in male banana flower explants was MS medium supplemented with 2 ppm 2,4-D and 5 ppm BAP. This medium resulted in calluses that exhibited a yellowish-white color and a compact texture. Keywords: callus morphology; genetic improvement; plant growth regulator; tissue culture; triploid banana
Evaluation of phosphorus fertilizer rate based on Upland Soil Test Kit analysis for tomato fertigation Alveno, Vitho; D. Susila, Anas; Suketi, Ketty; Maharijaya, Awang
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 52 No. 2 (2024): Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24831/jai.v52i2.52177

Abstract

Upland Soil Test Kit (PUTK) is a rapid soil analysis kit that estimates the nutrient availability in soil. From previous studies, tomato plant production showed a good correlation with available phosphorus (P) and potassium (K) analysis using the PUTK. This study aimed to find the optimal P fertilizer rate based on PUTK analysis for tomato fertigation. This experiment was carried out using a randomized complete block design, four replications with a single factor, namely P fertilizer rate comprising 0% X, 50% X, 100% X, 150% X, and 200% X, with X the recommended P fertilizer of PUTK on moderate P availability (175 kg ha­1). The ANOVA showed that P fertilizer did not significantly affect the vegetative growth, production, and fruit quality of tomatoes. It means that PUTK analysis had low accuracy as a basis for determining the optimum P fertilizer rate for tomato fertigation. The low accuracy could be due to PUTK underestimating the status of soil P availability. Mechlich-1 analysis showed that the experimental field had high P availability (44.6 ppm), while PUTK analysis still indicates moderate P availability. This shows that PUTK needs to be developed further, especially quantifying the result to help make informed and accurate decisions. Keywords: precision farming; precision fertilization; Upland Soil Test Kit; FERADS; sustainable agriculture
Seed tuber production of potato from stem cuttings, planting densities, and paclobutrazol concentrations Kalsum Yulifar, Andi Sri Ummi; Maharijaya, Awang; Purwito, Agus; Gunawan, Endang; Harti, Heri; Suhartanto , M. Rahmad
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 52 No. 1 (2024): Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24831/jai.v52i1.52685

Abstract

Rapid multiplication of potato seed using cuttings is a pivotal strategy for ensuring seed availability. This study aimed to elucidate the interactive effects of node number, paclobutrazol concentration, and planting density on seed potato and cutting productions. The study consisted of two experiments. The first experiment evaluated two genotypes (Granola and PKHT-6) with one or two nodes per cutting. The second experiment investigated planting density (one, three, or five plants per polybag) and paclobutrazol concentration (0, 15, 30, and 45 mg L-1). Notably, node number did not significantly influence cutting success, despite genotype-specific and interactive effects on vegetative parameters like plant height, stem diameter, leaf number, and root length. Planting density had no statistically significant impact on all vegetative parameters but demonstrably affected all production parameters. Conversely, paclobutrazol concentration significantly affected all vegetative parameters and influenced all production parameters except tuber weight per plant. From the results of this research, the use of single cutting is more recommended because it will obtain more plant material compared to two-node cuttings. Thus, in a more global context of potato development, this has the potential to increase the production of potato seeds from cuttings twice as much as the current seed production. The use of three plants/polybags is more recommended since it will produce more tuber but not different with five plants, and the use of paclobutrazol 15 mg L-1 is also recommended to increase the number of tubers. In this way, the production costs of G0 potato seeds can be reduced so that the availability of G0 potato seeds will be more guaranteed and affordable. Keywords: Granola; PKHT-6; rapid multiplication; tuber production
Aksi Gen Epistasis Duplikat pada Karakter Terkait Toleransi Naungan di Tanaman Tomat Ritonga, Arya W; Syukur, Muhamad; Chozin, Muhammad Achmad; Maharijaya, Awang; Sobir, Sobir
Buletin Agrohorti Vol. 12 No. 2 (2024): Buletin Agrohorti
Publisher : Departemen Agronomi dan Hortikultura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agrob.v12i2.56491

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Informasi tentang karakter seleksi dan pewarisan sifatnya sangat penting bagi program pemuliaan tanaman. Penelitian ini bertujuan untuk memperoleh karakter seleksi dan informasi pewarisan sifatnya terhadap cekaman naungan pada tanaman tomat. Populasi persilangan Biparental SSH3 x 4979 (tetua, F1, backcross dan F2) ditanam pada kondisi tanpa naungan (N0) dan kondisi cekaman naungan paranet 50% (N50) di Kebun Percobaan Pusat Kajian Hortikultura Tropika – Institut Pertanian Bogor, Pasir Kuda, Bogor, Jawa Barat, Indonesia. Hasil penelitian memperlihatkan bahwa karakter kehijauan daun, fruit set, jumlah buah dan bobot buah per tanaman merupakan karakter seleksi toleran naungan pada tanaman tomat. Terdapat aksi gen dominan dan epistasis duplikat pada karakter-karakter tersebut baik pada kondisi normal maupun kondisi cekaman naungan kecuali karakter fruit set pada kondisi tanpa naungan. Dominansi pada karakter-karakter tersebut bersifat over dominan pada kondisi tanpa naungan, namun dominan parsial pada kondisi cekaman naungan. Ragam aditif lebih berperan dibandingkan ragam non aditif pada karakter kehijauan daun, fruit set, jumlah dan bobot buah per tanaman pada kondisi normal namun sebaliknya dimana ragam non aditif lebih berperan dibandingkan ragam aditif pada karakter-karakter tersebut saat kondisi cekaman naungan. Seleksi pada generasi awal seperti generasi F2 dapat efektif dilakukan pada karakter-karakter tersebut pada kondisi tanpa naungan, namun seleksi pada kondisi cekaman naungan terhadap karakter-karakter tersebut lebih baik dilakukan pada generasi lanjut. Kata kunci: agroforestri, berkelanjutan, tumpang sari, varietas unggul
MUTU FISIK DAN KIMIA GABAH DAN BERAS GALUR – GALUR DIHAPLOID PADI HITAM Danu Kuncoro; Iswari Saraswati Dewi; Willy Bayuardi Suwarno; Awang Maharijaya; Purwoko, Bambang Sapta
Jurnal Agrotek Tropika Vol. 13 No. 4 (2025): JURNAL AGROTEK TROPIKA VOL 13, NOVEMBER 2025
Publisher : Departement of Agrotechnology, Agriculture Faculty, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jat.v13i4.9713

Abstract

Beras berwarna, khususnya beras hitam, merupakan jenis beras fungsional yang kaya akan antosianin dan memiliki nilai gizi tinggi. Seiring meningkatnya kesadaran masyarakat terhadap pola hidup sehat, permintaan terhadap beras hitam pun meningkat. Namun, Indonesia saat ini baru memiliki satu varietas unggul padi hitam, yaitu Jeliteng, sehingga diperlukan alternatif varietas baru yang memiliki mutu fisik dan kimia gabah serta beras yang baik. Salah satu sumber potensial adalah galur-galur dihaploid hasil persilangan melalui kultur antera, tetapi informasi mengenai mutu fisik dan kimia gabah serta berasnya masih terbatas, padahal data tersebut penting untuk mendukung seleksi calon varietas unggul yang adaptif dan disukai konsumen. Penelitian ini bertujuan untuk mengevaluasi mutu fisik dan kimia gabah serta beras dari galur-galur dihaploid padi hitam hasil kultur antera. Materi genetik terdiri atas 14 galur dihaploid dan dua varietas pembanding, yaitu Jeliteng (beras hitam) dan Aek Sibundong (beras merah). Pengujian meliputi karakter fisik (rendemen beras kepala, rendemen beras pecah kulit, panjang dan bentuk beras, persentase butir kapur, dan warna aleuron), kimia (kadar amilosa dan suhu gelatinisasi), serta uji organoleptik nasi (tekstur, rasa, aroma, dan penampilan). Hasil penelitian menunjukkan bahwa enam galur (DK-2, DK-8, DK-9, DK-11, DK-12, dan DK-14) memiliki kadar amilosa lebih rendah dan rendemen beras lebih baik dibanding Jeliteng. Dua galur, DK-6 dan DK-7, menghasilkan nasi lebih pulen dengan kadar amilosa 18,41% dan 19,11%, serta rendemen beras pecah kulit masing-masing sebesar 68% dan 66%. Temuan ini menunjukkan potensi beberapa galur dihaploid sebagai calon varietas unggul padi hitam yang adaptif dan disukai konsumen.
Perbanyakan Ruskus (Ruscus hypophyllum L.) secara In Vitro Agus Purwito; Prima Muklisa; Awang Maharijaya
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 33 No. 2 (2005): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1901.44 KB) | DOI: 10.24831/jai.v33i2.1519

Abstract

These experiments were aimed to obtain optimum medium for micropropagation of Ruscus. There were two experiments consist of in vitro shoots proliferation, shoot elongation and rooting.  The experiment of shoot proliferation performed by inducing adventitious shoots from explant in the Murashige and Skoog (1962)(MS) basal medium supplemented with combination of plant growth regulators BAP (0.0, 0.5, 1.0, 2.0, 4.0 and 6.0 mg/l) and IAA (0.0, 0.1, 0.2 and 0.4 mg/l). The elongation and rooting of plantlets were induced in the different concentration of the MS basal medium (0.5, 1.0 and 2.0 strength) combined with IBA (0.0, 1.0, 2.0, 3.0 and 4.0 mg/l).  Both experiments were arranged as completely randomized design with 15 replications. Adventitious shoots were produced in all medium supplemented with BAP with or without IAA. However MS medium supplemented with BAP 1 mg/l or 2 mg/l combined with IAA 0.2 mg/l were the best.  The number of adventitious shoots in these medium were 9.2 and 9.4 shoots after 8 weeks cultured respectively.  Increasing concentration of BAP more than 4 mg/l decreased number and size of adventitious shoots.  The plantlets produced in the proliferation medium were then transferring to the next treatments for elongation and rooting.  The best medium for elongation and rooting were medium with half strength of MS with or without IBA. Acclimatization conducted by transferring the rooted plantlets on the medium containing sterilized soil and rice husk charcoal (1:1).  After 4 weeks acclimatization, 60-100 percent of plantlets were survived and growth, depend on treatments.   Key words: Acclimatization, adventitious shoot, micropropagation, rooting, Ruscus hypophyllum L.
Uji Ketahanan in Vitro Klon-klon Kentang Hasil Persilangan Kentang Kultivar Atlantic dan Granola terhadap Penyakit Layu Bakteri (Ralstonia solanacearum) dan Busuk Lunak (Erwinia carotovora) Awang Maharijaya; Muhammad Mahmud; Agus Purwito
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 36 No. 2 (2008): Buletin Agronomi
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (50.137 KB) | DOI: 10.24831/jai.v36i2.20486

Abstract

Several bacterial pathogens can cause diseases of potato. Ralstonia solanacearum and Erwinia carotovora are those of the world’s most important diseases of potato, especially in tropical climates. This experiment was aimed to obtain putative potato cultivars having good tolerance to bacterial wilt caused by R. solanacearum and soft rot caused by E. carotovora. Two adopted potato cultivars in Indonesia, cv. Atlantic (2n=4X=48) and cv. Granola (2n=4X=48), were used as parents. The experiment was arranged in a Randomized Complete Design. Test for tolerance was perfomed in vitro using BF15 as susceptible control and Solanum stenotonum as tolerance control. The result showed that there was high diversity of tolerance level to both R. solanacearum and E. carotovora. Some clones showed good tolerance level as compared to cv. Atlantic and cv. Granola, but none showed that as compared to S. stenotonum.
Co-Authors ,, Rosminah Abdul Hakim Ady Daryanto Afaf, Sausanil Agus Purwito Ahmad Fauzi Ridwan Alveno, Vitho Amalia Nurul Huda Amarilis, Shandra Amrullah, Rizki Abi Anas Dinurrohman Susila Arum Sholikhah Azhari, Andi Bagas Akmala Putra Bambang Sapta Purwoko Bonjok Istiaji Danu Kuncoro Dhiya Nabilla Ardhani Diny Dinarti Diyah Martanti, Diyah Dwinita Wikan Utami Eka Fatmawati Tihurua Endang Gunawan Eny Widajati Erna Siaga, Erna Erna Sinaga Erviana Eka Pratiwi Fazat Fairuzia Hariyadi Harti, Heri Hidayat, dan Purnama Ika Roostika Inayah Yasmin Kamila Iswari Saraswati Dewi Iswari Saraswati Dewi Ita Aprilia J. K. J. Laisina Juharni Kalsum Yulifar, Andi Sri Ummi Ketty Suketi Kristianto Nugroho Kunio Yamada Kusmana Kusuma Darma Kusuma Darma Lina Herlina Lina Herlina M A Chozin Marlin Marlin Masaki Ochiai Megayani Sri Rahayu Muhamad Syukur Muhammad Mahmud Muhammad Ridha Alfarabi Istiqlal Neng Neni Neni, Neng Ni Made Armini Wiendi Ni Made Armini Wiendi, Ni Made Niken Kendarini Nina Agusti Widaningsih Prawestri, Apriliana Dyah Prima Muklisa PURNAMA HIDAYAT Purwoko, Bambang Sapta Purwoko Qadir, Abdul Rahmawati, Rika Sri Reflinur Reflinur Reflinur Reflinur Rerenstradika T. Terryana Ria Rif’atunidaudina Riti, Estriana Ritonga, Arya W Ritonga, Arya Widura Rizki Abi Amrullah Romdoni, Arif Rosminah, Rosminah Salma, Linda Nur Sani, Mutiyara Sari Mahyendra Siswahyudianto Siti Yuliani, Titiek Sobir Sobir Sobir Sobir Sobir Sobir Sobir Sobir Suhartanto , M. Rahmad Sukma, Dewi Sulassih, . Suryo Wiyono Suwarto Suwarto Syarifah Iis Aisyah Tamrin Khamidi Tamrin Khamidi Tengku Laila Kamaliah Tri Handayani Ulil Azmi Usep Jenudin Willy Bayuardi Suwarno Witjaksono Yudiwanti Wahyu E. Kusumo Yuliani, Titiek