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Yoserizal Yoserizal
Andalas University
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Journal : Microbiology Indonesia

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Selection and Identification of Cellulase-Producing Bacteria Isolated from the Litter of Mountain and Swampy Forest . WIZNA; HAFIL ABBAS; YOSE RIZAL; ABDI DHARMA; I PUTU KOMPIANG
Microbiology Indonesia Vol. 1 No. 3 (2007): December 2007
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (68.383 KB) | DOI: 10.5454/mi.1.3.7

Abstract

The isolation and selection of cellulase-producing bacteria was conducted to identify the species of cellulolytic Bacillus. The bacteria were isolated from the litter of swampy forest in Pesisir Selatan and mountain forest in Lembah Anai Tanah Datar. These bacteria were cultivated in selective media to obtain bacteria from the genus Bacillus. Six Bacillus isolates were obtainedfrom swampy forest and three Bacillus isolates from mountain forest. These isolates were cultivated in agar medium with carboxymethylcellulose as the carbon source. Colonies which produced clear zones were assumed to be cellulolytic Bacillus. Based on biochemical and morphological examinations the result indicated that these two isolates were Bacillus coagulans and B. amyloliquefaciens. The cellulase activity of B. coagulans and B. amyloliquefaciens were 0.812 and 1 200 unit ml-1 to C1(b-exoglucanase) respectively, 0.368 and 0.488 unit ml-1 to Cx(b-endoglucanase) respectively.
Co-Authors Abdi Dharma Agus Nurofik Ahyani, Tanthowi Jauhari Akhis Soleh Ismail Andre Zubir Anwar Kasim Aprizal Aprizal Aranzi Eriza Arwin Arwin Arya Azra Leswara Asrinaldi Asrinaldi Aulia Putri, Anisa Cecilya Asmarani Oka Putri Cinditha Fianda Dachi, Theofilus Desna Aromatica Dessy Trismiyanti Dhea Yosfika Difatria Nurdin Dwiputra, Rayhan Arif Erien Sri Wahyuni Eriza, Aranzi Erlina Erlina Firdausi, Fakhri Ahmad Firmanto Firmanto Hadiyanto Hadiyanto HAFIL ABBAS Hanoselina, Yulia Harni, Harni Hasan Sazali Hatika, Rindi Hendra Hendra Hendra Hendra Hermiyetti Hernadewita, Hernadewita Hs, Putri Hidayah Hutabian, Edwin I PUTU KOMPIANG Iip Permana Indra Jaya Juwita, Della Kadran Fajrona Kadri, Hanif Al Krismena Tovalini Laksana, Wanda Mabrukah Tomimi, Zahran Madeleine Cania MARIA ENDO MAHATA Marlinda Marlinda Melati Sukma Miftahul Syafira Mirnawati Mirnawati Muh Ajri Halim Muhammad Umar Maya Putra Naufal Azmi “Chan Nazar, Armenia Nellitawati Nellitawati Nila Wahyuni Ningsih, Dina Gustia Nini, Nini Pebriyanto S Prianka, Marselia Febi Purwo Subekti Putra, Trimardi Jaya Putri, Cecilya Asmarani Oka Rahmadhona Fitri Helmi Rahman, Jhoni Rahmi, Ulfia Retno Kus Setyowati Ridwan , Muhammad Rifma Rifma Rizka Khairunnisa Roni Ekha Putera Rr. Endang Sri Sulasih Rusdianto, Nur S, Pebriyanto Sintya Trifira Sintya Trifira Siti Rizka Mulyani Sohibun -, Sohibun Sufyarma Marsidin Sulastri Sulastri Suripto, Heri Sutiyem, Sutiyem Taufani Ahmad Tirza Haqia Purnama Tovalini, Krismena Vovi Ratna Qori Wahyuni, Erien Sri Weni Artitantia Wewen Kusumi Rahayu Wike Yuliana Wizna Wizna Yessie Aldriani Yolani Utami Yulia Hanoselina Yulivestra, Malse