Article Per Year (5 Year)
p-Index From 2021 - 2026
1.556
P-Index
This Author published in this journals
All Journal Althea Medical Journal Dharmakarya : Jurnal Aplikasi Ipteks Untuk Masyarakat Jurnal Teknologi Informasi dan Ilmu Komputer Global Medical and Health Communication Molecular and Cellular Biomedical Sciences (MCBS) Chimica et Natura Acta Jurnal Endurance: Kajian Ilmiah Problema Kesehatan BALABA (JURNAL LITBANG PENGENDALIAN PENYAKIT BERSUMBER BINATANG BANJARNEGARA) The Indonesian Journal of Gastroenterology, Hepatology and Digestive Endoscopy
Savira Ekawardhani
Division Of Parasitology Department Of Biomedical Science Faculty Of Medicine Universitas Padjajaran, Jalan Raya Bandung Sumedang KM. 21, Hegarmanah, Kecamatan Jatinangor, Kabupaten Sumedang, Jawa Barat, Indonesia
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Dentistry Economics, Econometrics & Finance Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health Veterinary

Published : 13 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 13 Documents
Search

 1   2 
The μDrop Method Enhances Melanin Content Measurement in the in vitro Melanogenesis Model Using B16F10 Cell Line Yuliarni, Dinda; Kamilah, Mutiara Mila; Nugraha, Gaga Irawan; Faridah, Lia; Bashari, Muhammad Hasan; Ekawardhani, Savira
Molecular and Cellular Biomedical Sciences Vol 9, No 1 (2025)
Publisher : Cell and BioPharmaceutical Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21705/mcbs.v9i1.527

Abstract

Background: The B16F10 cell line is a cell frequently used in melanin content assays. However, reports on cell models using B16F10 are limited, particularly as the robust model cell in the Indonesian cosmetics industry. We found measuring melanin content using microplate spectrophotometry to be challenging, so this research was conducted to develop a method using μDrop spectrophotometry.Materials and methods: In this in vitro study, the B16F10 melanoma cell line was cultured in Roswell Park Memorial Institute (RPMI) medium containing 5% fetal bovine serum (FBS). The cells were categorized into control, stimulated, and treated groups. Melanogenesis stimulation was achieved using 1μM α-melanocyte-stimulating hormone (α-MSH), while inhibition using 800 μg/ml kojic acid. After treatment, the cells were incubated for 48 hours. Their melanin content was then measured using an ELISA reader with a μDrop method and compared with the microplate method. Statistical analysis used a one-way ANOVA test with Turkey’s Post Hoc analysis.Results: The μDrop method increased the melanin signal into the linear range of machine readings, while the signals from the microplate method fell far below this range. The B16F10 melanoma cell lines stimulated by α-MSH exhibited increased melanin production compared with the control group, while kojic acid treatment significantly reduced (p<0.05) melanin content in the stimulated group.Conclusion: The μDrop method significantly outperformed the microplate method in measuring melanin content within melanogenesis cell models, offering enhanced accuracy and particularly excelling at quantifying low content of melanin. Keywords: μDrop, microplate, melanin, melanogenesis, B16F10 cell line, RPMI
New Custom Primers for the Detection of SARS-CoV-2 using the Singleplex rRT‒PCR SYBR Green-Based Method with the NSP10 and N genes as Targets Gaffar, Shabarni; Shabrinna, Hanif; Putri, Rafika; Wiraswati, Hesti Lina; Hartati, Yeni Wahyuni; Ishmayana, Safri; Faridah, Lia; Ekawardhani, Savira
Chimica et Natura Acta Vol 13, No 1 (2025)
Publisher : Departemen Kimia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cna.v13.n1.53493

Abstract

Although COVID-19 is no longer a global health emergency, rapid, sensitive, and specific detection tests are still needed. In this study, we developed a cost-effective test, the SYBR Green-based rRT‒PCR kit, using new custom primers targeting the N and NSP10 genes of the SARS-CoV-2 virus. The specificity of the designed primers was determined through agarose gel electrophoresis. A standard curve generated from a ten-fold dilution of SARS-CoV-2 RNA was used to determine the efficiency and sensitivity of the kit. Validation of this protocol was carried out on ten clinical specimens. As expected, the results showed that the N and NSP10 gene primers produced 134 and 161 bp products, respectively. The limits of detection and limit of quantification with N gene primers were 7.74 and 23.46 copies/μL, respectively, and those with the NSP10 gene primers were 4.69 and 14.21 copies/μL, with a PCR efficiency of 102.5% and 110.6%, respectively. The validation results with clinical specimens revealed that seven samples were true-positive for COVID-19 (Ct range 15.09–21.33), and three were confirmed to be true-negative. Costs associated with COVID-19 patient testing can be anticipated to decrease with the use of custom primers for the detection of SARS-CoV-2 via the use of the singleplex rRT‒PCR mix SYBR Green.
Informasi Dini Terhadap Penyakit Kanker Payudara berbasis Telepon Pintar Wiraswati, Hesti Lina; Ekawardhani, Savira; Windria, Sarasati; Khaq, Arina Al; Nurfitri, Fauzia; Wahyuni, Dini; Rachmawati, Nadya; Baasin, Rusdi Toriq; Arimdayu, Annissa Retno; Arimdayu, Annisa Retno
Jurnal Teknologi Informasi dan Ilmu Komputer Vol 9 No 4: Agustus 2022
Publisher : Fakultas Ilmu Komputer, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25126/jtiik.2022933182

Abstract

Latar Belakang: Deteksi dini kanker payudara akan memberikan tingkat kesembuhan lebih tinggi bagi penderita jika penderita datang lebih dini ketika terdapat gejala adanya kanker payudara. Namun, masyarakat Indonesia cenderung datang ke pusat kesehatan dalam kondisi kanker stadium lanjut sehingga sulit diobati. Ketersediaan layanan informasi tentang kanker payudara, dan pemeriksaan mandiri berbasis telepon pintar menjadi kebutuhan saat ini.  Oleh karena itu diperlukan sosialisasi yang benar tentang kanker. Salah satu alat sosialisasi yang dianggap efektif adalah media digital. Di Indonesia, aplikasi berbasis telepon pintar sudah dibuat untuk kanker serviks, tetapi untuk kanker payudara hanya tersedia dalam bentuk hologram berisi simulasi gerakan Periksa Payudara Sendiri (SADARI). Aplikasi hologram ini belum menyertakan informasi tentang kanker payudara secara komprehensif. Tujuan: Menyediakan layanan informasi tentang kanker payudara, dan penjelasan serta simulasi bagaimana memeriksa secara mandiri yang berbasis menggunakan aplikasi berbasis telepon pintar. Metode: Metode penelitian terdiri atas desain aplikasi, pengumpulan asset sebagai sumber database, validasi aplikasi, dan uji coba aplikasi kepada 100 responden menggunakan teknik purposive sampling. Evaluasi kelayakan aplikasi dilakukan menggunakan kuesioner pre-test dan post-test. Hasil: Aplikasi berhasil dibuat dan dapat diunduh melalui google play store. Aplikasi ini membantu meningkatkan pengetahuan responden tentang kanker dari rata-rata 43,38% menjadi 77,72%. Sebesar 34.6% responden setuju dan  61.8% sangat setuju bahwa aplikasi ‘Be Care’ berguna dan bermanfaat. Kesimpulan: Aplikasi “Be Care” dinilai layak untuk digunakan dalam meningkatkan pengetahuan, kesadaran, dan keterampilan terkait deteksi dini kanker payudara. Aplikasi berbahasa Indonesia dengan status ‘free access’ ini diharapkan menjadi salah satu media efektif yang berkontribusi dalam menurunkan jumlah kasus baru dan kematian akibat kanker. AbstractBackground: An early breast cancer detection will provide a higher cure rate for sufferers if the patient arrives early when there are symptoms of breast cancer. However, Indonesians tend to come to the health centres with an advance condition, which it makes difficult to be treated. The availability of information about breast cancer and smartphone-based self-examination is a necessity at this time. Therefore, we need the correct socialization. One of the socialization tools that are considered effective is digital media. In Indonesia, smartphone-based application has been made for servical cancer, but it is only available in hologram containing of Breast Self-Examination (BSE) for breast cancer. This does not include comprehensive information about breast cancer. Objective: To provide information service about breast cancer, and an explanation also simulation of how to do BSE based on a smartphone-based app. Methods: The research consists of application design, collection of assets as a database source, application validation, and testing to 100 respondents using purposive technique sampling. The evaluation for feasibility of the app was carried out using pre-test and post-test questionnaire.  Results: The app was successfully created and can be downloaded via Google Playstore. This app helps increase knowledge of the respondents about cancer from an average 43.38% to 77.22%. As much as 34.6% of respondents agree and 61.8% strongly agree that the app “Be Care” is useful. Conclusion: "Be Care" app is considered feasible to be used in increasing knowledge, awareness, and skills related to early detection of breast cancer. This Indonesian language app with “free access” is expected to be one of the effective media contributing to reducing the number of new cases and death due to cancer.
Co-Authors Achadiyani Ade Zuhrotun Adelina Siagian Agoes, Ridad Andri Rezano Arimdayu, Annisa Retno Arimdayu, Annissa Retno Asep Wiryasa Astri Gloria Larwuy Baasin, Rusdi Toriq Bashari, Muhammad Hasan Dewi, Sumartini Dicky Bagus Pratama Dini Oktaviani Dini Wahyuni Djati, Imam Damar Enny Rohmawaty Erlangga Ing Geni Bisma Pratama Eva Nuriyah, Eva Fahmy Fathurrohman Fauzah, Nisa Fauziah, Nisa Fauziah, Nisa Gaga Irawan Nugraha Hadyana Sukandar Hansen Wangsa Herman Hesti Lina Wiraswati I Made Joni Ida Kinasih Kamilah, Mutiara Mila Khaq, Arina Al Latama, Zahra Nabila Lia Faridah Liana Awalia Lutfunnisa Marhendra Satria Utama Melia Juwita Adha Meri Alex Sandra Muhammad Begawan Bestari Muhammad Saifuddin B. S. Arif Nenny Agustanti Nurfitri, Fauzia Nurul Mufliha Patahuddin PUTRI, RAFIKA Rachmawati, Nadya Ramadhani Eka Putra Rini Hendriani Rosdianto, Aziiz Mardanarian Safri Ishmayana Salma Nur Raidah Saragih, Winda A. M. Sarasati Windria Shabarni Gaffar -, Shabarni Gaffar Shabrinna, Hanif Siti Silvia Nur Shofa Shamantri Sulaksono, Haura Labibah Salsabil Syafrizal Saragih Usman, Hermin A. Veronica Oladitha Siagian Watanabe, Kozo Wijaya, Muhammad Palar Yasfira Aradella Yeni Wahyuni Hartati Yoga Windhu Wardhana Yuliarni, Dinda