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INDONESIA
Bioscientist : Jurnal Ilmiah Biologi
ISSN : 23385006     EISSN : 26544571     DOI : https://doi.org/10.33394
Bioscientist : Jurnal Ilmiah Biologi (BJIB), receiving and publishing article in the form of research (scientific article) in the field of biology education and utilization of biological research in learning. Moreover, this journal bridges the gap between research and practice, providing information, ideas and opinion, in addition to critical examinations of biology research and teaching. Through the coverage of policy and curriculum developments, the latest results of research into the teaching, learning and assessment of biology are brought to the fore. Special emphasize are as follow: 1. Research on Learning Biology (Biology Learning Materials at All Education Levels). 2. Pure Research of Biology Developed or Studied to the Sources, Materials, or Instructional Media Biology (Biology Learning Materials at All Education Levels and Application in Society). 3. Curriculum of Biology Education at all Education Levels. 4. Class Action Research (CAR) and Lesson Study in Biology. 5. Other Qualitative Research of Biology Education. 6. The School Management and Biology/Science Laboratory Management. 7. Biology Learning Evaluation. 8. Teacher Professional Issues/Trends in Biology Education. 9. Another Study for the Scope of Biology Education. 10. Research on Environmental Education.
Articles 115 Documents
Search results for , issue "Vol. 12 No. 2 (2024): December" : 115 Documents clear
Dampak Pemanfaatan Elektronik Rekam Medis di Fasilitas Kesehatan: Pendekatan Sistematik Literatur Review Arsyam, Haeril; Sulaiman, Lalu; Setiawan, Sabar
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.12800

Abstract

This study examines the impact of Electronic Medical Records (ERM) implementation in healthcare facilities over the last decade, focusing on its benefits and challenges. ERM has significantly improved patient care quality, operational efficiency, and data security, providing fast and accurate access to medical information for clinical decision-making. However, despite these advantages, the adoption of ERM faces several technical and organizational challenges, including limited interoperability between systems and user resistance. A systematic literature review was conducted, analyzing studies from 2014-2024, to explore both the positive outcomes and barriers encountered during ERM implementation. The results indicate that while ERM reduces medical errors and enhances care coordination, particularly in managing chronic conditions, issues such as data privacy concerns and high implementation costs remain significant barriers. Recommendations to address these challenges include the development of standardized policies for system interoperability and comprehensive user training to facilitate adaptation. This study underscores the need for a more integrated approach that combines technical improvements with organizational support to maximize the potential benefits of ERM.
mRNA Relative Expression Catalase in Hypertension Yohana, Yohana; Faradilla, Meutia Atika; Meiyanti, Meiyanti; Hartanti, Monica Dwi; Margo, Eveline; Anastasya, Karina Shasri
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.12825

Abstract

Hypertension is number one worldwide disease which lead to death. Initially hypertension is caused by changes in the walls of blood vessels. These vascular changes are due to the accumulation of ROS (Reactive Oxygen Species). ROS is the result of the balance between antioxidants and oxidants, but uncontrolled conditions will cause vascular damage. One of ROS product from metabolism which could played role in downregulation antioxidant gene expression is hydrogen peroxide. Hydrogen peroxide could be neutralized by catalase enzyme. Studies about catalase expression in hypertension subject is still limited. This research aims to determine mRNA relative expression catalase in hypertension. In this case control study, thrity subject hypertension and normotension subject were recruited within the age of 50-60 years. Hypertension subject was chosen according to JNC 8. Two millilitres vein blood was isolated into RNA. mRNA expression was detected by qRT PCR with 2 steps. Relative expression was determined using livak method. The data was analyzed using Mann Withney test with GraphPad software. Research results show that (1) catalase mRNA relative expression was 0,6 fold in hypertension; (2) catalase relative expression was not significantly lower in hypertension; (3) other antioxidants might have other mechanism to detoxify ROS and prevent hypertension.
Analisis Efektivitas Pelatihan Full Online dan Blended Learning di Balai Pelatihan Kesehatan Mataram Musawirin, Musawirin; Menap, Menap; Sismulyanto, Sismulyanto
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.12878

Abstract

Penelitian ini bertujuan untuk mengetahui efektivitas pelatihan full online dan blended learning di Balai Pelatihan Kesehatan Mataram. Studi ini merupakan penelitian kuantitatif untuk membandingkan peningkatan pengetahuan peserta berdasarkan hasil pre-test dan post-test dari metode pelatihan full online dan blended learning. Analisis data penelitian menggunakan statistik inferensial uji-t untuk mengetahui perbedaan hasil belajar kedua metode pelatihan. Hasil penelitian menunjukkan bahwa (1) tidak ada perbedaan signifikan antara nilai pre-test dan post-test peserta, dengan p-value lebih besar dari 0,05; (2) peserta yang mengikuti pelatihan full online menunjukkan persentase peningkatan sebesar 75,41%, lebih besar dibandingkan dengan metode blended learning sebesar 60,23%. Hal ini mengindikasikan bahwa fleksibilitas dalam metode full online dapat memberikan dampak yang lebih baik terhadap peningkatan pengetahuan peserta.
Teknik RAPD (Random Applied Polymorphic DNA) untuk Analisis Keanekaragaman Genetik pada Tanaman Ciplukan (Physalis angulata L.) di Kabupaten Labuhan Batu Utara Yusvita, Diana; Idami, Zahratul
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.12889

Abstract

The ciplukan plant (Physalisangulata L.) can be used as traditional medicine, but so far people only know that this plant can only be used for the fruit. This research aims to determine the results of RAPD primary analysis which can produce polymorphic bands in Ciplukan Plants (Physalisangulata L.) in North Labuhan Batu Regency using different primers. This research is a quantitative research. Sampling was carried out in Bondar Village and Simpang Tiga Village, Kualuh Leidong District, North Labuhan Batu Regency. Then continued with molecular analysis at the Genetics Laboratory, Faculty of Science and Technology, North Sumatra State Islamic University, Medan. The results of RAPD (Random Applied Polymorphism DNA) analysis of ciplukan plants in North Labuhan Batu district using six different primers, namely the length of the nucleotides in the amplified DNA fragments, are shown in primers OPA-2 & OPA-3 are 600bp-3000bp, while OPA-5, OPA-7, OPD -11, & OPD-13 are 500bp-3000bp with different nucleotide sequences. The differences in the length and number of nucleotide sequences in ciplukan plants are caused by the plants used coming from different locations and several factors such as the environment, population size, natural conditions, reproductive methods and natural selection.
Potensi Infusa Daun Paitan (Tithonia diversifolia) sebagai Larvasida Terhadap Mortalitas dan Morfologi Larva Aedes aegypti Wahyuni, Dwi; Swandono, Hari Untarto; Purwani, Asih Imulda Hadi; Pramasari, Nadia; Putri, Sherlyna Rizkiya
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.12902

Abstract

Dengue Hemorrhagic Fever is an infectious disease caused by the Dengue virus through the Aedes aegypti mosquito vector, which is controlled both at the larval and adult stages. The use of chemicals that are difficult to degrade in nature can pollute the environment. The Paitan plant (Tithonia diversifolia) contains secondary metabolite compounds that have the potential to be natural larvicides. This study aims to determine the natural larvicidal activity of Paitan leaf infusion. This study is an experimental study. The results of this study indicate that (1) Paitan leaf infusion contains alkaloid, flavonoid, terpenoid, saponin, phenolic, and tannin compounds. At concentrations of 1.8%, 3.2% and 5.6%, the mortality percentage results were 41.30%, 50.60% and 60% respectively; (2) The administration of Paitan leaf infusion has an effect on morphological damage Aedes aegypti larvae in the thorax, digestive tract and siphon. The content of different metabolite compounds causes different levels of morphological damage at each concentration
Uji Efektivitas Ekstrak Daun Pandan Wangi (Pandanus amaryllifolius Roxb) dalam Sediaan Gel terhadap Bakteri Staphylococcus epidermidis Syafitri, Mulyani; Yuliana, Agnes; Krismayadi, Krismayadi
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.12903

Abstract

Jerawat atau acne vulgaris adalah peradangan kronis yang umum terjadi pada remaja dan dewasa, yang dapat menurunkan rasa percaya diri. Salah satu penyebabnya adalah bakteri Staphylococcus epidermidis. Daun pandan wangi (Pandanus amaryllifolius Roxb) mengandung senyawa flavonoid dan fenol yang berpotensi sebagai antibakteri dan dapat digunakan sebagai obat antijerawat. Gel adalah sediaan farmasi yang efektif untuk mengatasi jerawat. Penelitian ini bertujuan untuk mengetahui efektivitas antijerawat pada sediaan gel ekstrak daun pandan wangi terhadap bakteri Staphylococcus epidermidis. Studi ini merupakan penelitian eksperimental dengan melakukan uji antibakteri pada sediaan ekstrak daun pandan dan gel esktrak daun pandan wangi. Hasil penelitian menunjukkan bahwa (1) hasil uji efektivitas ekstrak daun pandan wangi dengan menggunakan metode sumuran pada konsentrasi 30%, 50%, dan 70% menghasilkan zona hambat berturut-turut adalah 12,48 mm, 16,69 mm, dan 17,68 mm sehingga konsentrasi 50% efektif menghambat bakteri Staphylococcus epidermidis; (2) hasil uji efektifitas gel ekstrak daun pandan wangi pada kosentrasi 15%, 20%, dan 25% menghasilkan zona hambat berturut-turut adalah 2,39 mm, 3,58 mm, dan 5,36 mm sehingga konsentrasi gel ekstrak daun pandan wangi 25% efektif menghambat bakteri Staphylococcus epidermidis. 
Daya Hambat Formula Optimum Sabun Cair Daun Bandotan (Ageratun conyzoides) terhadap Bakteri Staphylococcus aureus dan Pseudomonas aeruginosa Chandra, M. Andi; Afra, Fairuz Yaumil; Rahmiati, Nur
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.12914

Abstract

Infeksi bakteri lebih sering terjadi pada kulit karena kulit merupakan lapisan terluar tubuh. Ada bukti bahwa daun tanaman bandotan memiliki sifat antibakteri. Pengembangan formulasi sabun cair memungkinkan daun bandotan untuk mencapai potensi penuhnya. Penelitian ini bertujuan untuk mengetahui seberapa efektif sabun cair terhadap mikroorganisme Staphylococcus aureus dan Pseudomonas aeruginosa. Studi ini merupakan penelitian ekspirimen yang dilakukan di laboratorium untuk menguji aktivitas antibakteri sabun daun bandotan menggunakan difusi sumur pada bakteri Staphylococcus aureus dan Pseudomonas aeruginosa. Hasil penelitian menunjukkan bahwa bakteri Pseudomonas aeruginosa memiliki zona hambat sebesar 2 mm, yang termasuk kategori lemah, sedangkan bakteri Staphylococcus aureus memiliki zona hambat rata-rata sebesar 13 mm, yang termasuk kategori kuat. Dengan demikian, sediaan sabun cair memiliki nilai zona hambat rata-rata yang termasuk dalam kategori kuat terhadap bakteri Staphylococcus aureus. Nilai zona hambat rata-rata ketika diujikan terhadap bakteri Pseudomonas aeruginosa masuk ke dalam kategori aktivitas antibakteri yang kurang baik
Kandungan Kadar Protein Tempe yang Difortifikasi Coriandrum sativum, Alium sativum, dan Amomum cardamomum Khotimah, Khusnul; Hidayah, Maulida Ulfa; Raihan, Raihan; Windiani, Titi
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.12919

Abstract

Tempeh is an affordable source of plant-based protein for the community. The fortification of tempeh with coriander (Coriandrum sativum), garlic (Allium sativum), and cardamom (Amomum cardamomum) is important to study because these three spices contain bioactive compounds that potentially increase the protein content and nutritional value of tempeh. This research is necessary to scientifically examine the effect of spice fortification on tempeh protein levels as an effort to develop functional food. The testing method used in this study was the Kjeldahl method referring to SNI 01-2891-1992. Tempeh fortification with coriander showed the most significant increase in protein content, reaching 7.03%, compared to tempeh without spices, garlic, and cardamom, which only increased protein content to 2.74%, 2.92%, and 3.94%, respectively. This is due to the high protein content in coriander, approximately 17-20% per 100 grams of material, which significantly enriches the protein content of tempeh. In contrast, garlic and cardamom have lower protein content, around 6-7% and 9-11% per 100 grams of material, making their contribution to increasing tempeh protein content less significant. This research demonstrates that the use of coriander as a fortification ingredient can be an effective strategy to enhance the nutritional value and protein content of tempeh, making it a more functional and nutritious choice for consumers.
Keragaman Spesies Kupu-Kupu (Lepidoptera) Sebagai Objek Pengembangan Ekowisata TWA Suranadi Dan Upaya Penyusunan E-Modul Ekologi Hewan Efendi, Ismail; Karmana, I Wayan; Adawiyah, Siti Rabiatul; Arifin, Ahmad Aris
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.12921

Abstract

The purpose of this study is to identify the diversity of butterfly species as a potential object for ecotourism development in the TWA Suranadi and to develop an animal ecology e-module. This research is a descriptive exploratory study. Data collection was conducted using survey techniques, and samples were taken using Purposive Random Sampling by dividing the TWA Suranadi area into four transects (water transect, left transect, middle transect, and right transect). Butterfly collection was carried out using the sweeping technique implemented randomly. The development of the animal ecology module was designed using the 4D model (Define, Design, Develop, and Disseminate). The Develop phase was limited to expert validation to assess the content validity and construct validity of the animal ecology e-module. The results showed that butterfly diversity varied across the observation transects. The Middle Transect dominated with a high distribution percentage of 56.25%, followed by the Water Transect at 23.43%, the Left Transect at 15.62%, and the Right Transect with the lowest distribution percentage at 4.7%. Ecological analysis results indicated a species diversity index across the four research locations ranging at 2.341 (Moderate Diversity), an evenness index ranging from 0.152 (Low Evenness), and a dominance index of 0.108 (Low Dominance). Expert validation results showed that the content validity of the animal ecology e-module had an index of 0.880, categorized as very valid, while the construct validity, which included the feasibility of presentation, had an index of 0.902, categorized as very valid, and the feasibility of language aspect averaged at 0.770, categorized as valid.
Formulasi dan Uji Aktivitas Antibakteri Streptococcus mutans Pada Sediaan Obat Kumur Kombinasi Ekstrak Daun Seledri (Apium graveolens) dan Daun Jambu Biji (Psidium guajava) Yardha, Muahamad; Halimatushadyah, Ernie; Yuliana, Agnes
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.12933

Abstract

Dental caries is a disease of hard tissue caused by the activity of acid-producing bacteria that ferment carbohydrates after human consumption caused by the bacteria Streptococcus mutans. The flavonoid content in celery leaves and the tannin content in guava leaves can be used as an antibacterial treatment. Pharmaceutical preparations that can be used to treat dental caries problems are mouthwash. This research aims to formulate a combination of celery leaf and guava leaf extracts into a mouthwash preparation as an antibacterial against Streptococcus mutans. The research method used was experimental by testing the antibacterial activity of Streptococcus mutans in vitro. The results of the study showed that (1) the results of the antibacterial activity test on the negative control mouthwash were that the mouthwash base had no inhibition zone (2) the positive control chlorhexidine had an inhibition zone of 14.27mm (3) with a comparison between celery leaf extract and guava leaf extract 1:3 seeds, the results obtained were that the diameter of the inhibition zone at 20% concentration was 10.20 mm, 40% concentration was 11.20 mm, and 60% concentration was 12.05 mm; (4) The inhibitory power against Streptococcus mutans bacteria of the mouthwash containing a combination of celery leaf extract and guava leaf extract is greater than the inhibitory power of the positive control.

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