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INDONESIA
Bioscientist : Jurnal Ilmiah Biologi
ISSN : 23385006     EISSN : 26544571     DOI : https://doi.org/10.33394
Bioscientist : Jurnal Ilmiah Biologi (BJIB), receiving and publishing article in the form of research (scientific article) in the field of biology education and utilization of biological research in learning. Moreover, this journal bridges the gap between research and practice, providing information, ideas and opinion, in addition to critical examinations of biology research and teaching. Through the coverage of policy and curriculum developments, the latest results of research into the teaching, learning and assessment of biology are brought to the fore. Special emphasize are as follow: 1. Research on Learning Biology (Biology Learning Materials at All Education Levels). 2. Pure Research of Biology Developed or Studied to the Sources, Materials, or Instructional Media Biology (Biology Learning Materials at All Education Levels and Application in Society). 3. Curriculum of Biology Education at all Education Levels. 4. Class Action Research (CAR) and Lesson Study in Biology. 5. Other Qualitative Research of Biology Education. 6. The School Management and Biology/Science Laboratory Management. 7. Biology Learning Evaluation. 8. Teacher Professional Issues/Trends in Biology Education. 9. Another Study for the Scope of Biology Education. 10. Research on Environmental Education.
Articles 1,428 Documents
Pengembangan Protokol Deteksi Staphylococcus aureus Berbasis Molekuler Koentjoro, Maharani Pertiwi; Alviani, Melinda Nuril; Jatmiko, Yoga Dwi; Habibah, Laila Nur; Al Fatih, Ahmad Nuril Fuad; Kartikaningsih, Hartati
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.9494

Abstract

Staphylococcus aureus becomes a normal flora in humans, especially on the skin and in the nose. However, if it becomes excessive or if there are pathogenic variants, it can cause various health problems. The purpose of the study is to develop a molecular-based detection method for Staphylococcus aureus using the norA primer gene. The norA gene in Staphylococcus aureus is known to play a role in pathogenesis with its antibiotic resistance ability. This type of research is analytical observational with a cross-sectional method. The methods in the study include the isolation and identification of Staphylococcus aureus from healthcare facility equipment. Isolation and identification include bacterial isolation using Blood Agar Plate (BAP) media; isolate purification, Gram staining; biochemical tests using Mannitol Salt Agar (MSA) media, glucose tests, Voges Proskauer (VP) tests, catalase tests, and coagulase tests. Furthermore, S. aureus isolates were tested using a molecular-based method, namely Polymerase Chain Reaction (PCR). This method includes DNA isolation stages, qualitative testing with agarose gel electrophoresis, semi-quantitative testing with image J software, amplification with Real-Time Polymerase Chain Reaction (RT-PCR) using norA gene primers. The Mann-Whitney test results gave a value of p = 0.334 (p> 0.05) indicating the suitability between the culture method and the PCR method with the developed protocol in detecting Staphylococcus aureus. The developed method includes the use of base sequences in the norA gene primer, optimization of annealing and extension temperatures, and the concentration of DNA templates used.
Aplikasi Streptomyces sp. ASR 67 dan Pupuk Organik Cair dalam Mengendalikan Penyakit Layu Fusarium oxysporum dan Pemacu Pertumbuhan Tanaman Kentang (Solanum tuberosum L.) Varietas Granola Diharjo, Dewi; Fatmawati, Umi
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.9924

Abstract

Demand for the Granola variety of potatoes continues to increase, but its productivity is not yet sufficient for market needs. This is due to the wilt disease attack on potatoes caused by the fungus Fusarium sp., thereby reducing potato productivity. The aim of this research was to determine the use of liquid culture of Streptomyces sp. ASR 67 and liquid organic fertilizer as an organic anti-fungal in controlling wilt disease caused by Fusarium oxysporum on potato plants (Solanum tuberosum L.). The research was carried out using a Randomized Group Design (RAK) method using five treatments. Each treatment was repeated five times and each repetition used one potato plant. Treatment was given by watering the potato growing medium for 30 days, which was done every three days. The results showed that administration of liquid culture of Streptomyces sp. ASR 67 10% combined with liquid organic fertilizer (1:9) was able to increase plant growth with the parameters of height, number of leaves, number of stems, weight of tubers significantly, while POC 2% and Streptomyces sp. ASR67 (1:1) is able to reduce the incidence of wilt disease best.
Estimation of Ripitability, MPPA, Livestock Ranking and The Relationship of Morning and Afternoon Milk Production in The Second and Fourth Lactation Alhikami, Waliyyul Ahdi; Mudawamah, Mudawamah; Kentjonowaty, Inggit
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.12367

Abstract

Most Producing Production Ability (MPPA), also known as Expected Production Ability (EPA) in dairy cattle, indicates the ability to produce milk that can be passed down to offspring. The objective of this study is to obtain the repeatability values of morning and evening milk production in the second and fourth lactations of Friesian Holstein (FH) dairy cows as a basis for estimating MPPA values, ranking, and the relationship of milk production in livestock. This case study uses a sample determined by purposive sampling technique, focusing on cows with recorded second and fourth lactations. The research was conducted at a local dairy farm in the Pujon area of Malang Regency. The sample consisted of 12 cows with the same day, month, and lactation period. Data were analyzed using variance analysis, correlation, and regression. The results showed that the repeatability value of morning milk production ranged from 0,63 to 0,99, categorized as high, while the repeatability of evening milk production ranged from 0,39 to 0,99, categorized as moderate to high. The MPPA values from the study ranged from 3.602 liters per cow to 10,811 liters per cow (morning milk production) and from 1,237 liters/cow to 5,024 liters/cow (evening milk production). The ranking of cattle based on MPPA morning milk production ranged from 1 to 7. The relationship between morning and evening milk production is represented by the equation Y = 0,6213, X = 1,7587 with a determination coefficient of 88,68%.
Review : Kajian Potensi Bakteri Endofit sebagai Agen Biodegradasi Linear Alkylbenzene Sulfonate (LAS) Mahmudah, Arini Nurul; Faizah, Hanik; Munir, Misbakhul
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.10181

Abstract

Linear Alkylbenzene Sulfonate (LAS) is a type of anionic surfactant which is commonly used as a raw material in making commercial detergents or cleaners. Even though LAS can be degraded naturally in the environment, detergent wastewater containing LAS still needs to be treated properly before being discharged into the environment. One way that can be done to reduce the toxic effects of LAS is biodegradation. The use of bacteria, especially endophytic bacteria associated with plants, is known to have potential as a biodegradation agent for LAS. This review aims to examine the potential of endophytic bacteria as LAS biodegradation agents. The method used in this review is a literature review from several international and national journal references. Data were analyzed descriptively and presented in narrative and table form. The results of the research show that several species of endophytic bacteria isolated from various types of plants have the potential to degrade pollutants, such as heavy metals, benzene, phenol, phenanthrene (PHE), diesel, ammonium, phosphate, COD, and hydrocarbons which also shows that endophytic bacteria have the potential to degrades LAS.
Identifikasi Keragaman Mikroflora dan Pengaruhnya pada Kualitas Organoleptik Tempoyak Khas Suku Dayak di Kalimantan Tengah Swestyani, Sondra; Hujjatusnaini, Noor
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.11008

Abstract

Tempoyak as local wisdom and national culture is poorly documented. Therefore, it is important to explore the preferences of the millennial generation regarding the organoleptic qualities of Tempoyak. The research design is a one-shot case study. The variables tested were the diversity of microflora in tempoyak, and its influence on the level of organoleptic quality and the level of preference of the millennial generation. Identification of isolates using API-50CHL, biochemical tests and physiological tests. Organoleptic quality uses the hedonic scale test. The research panelists were 120 people from the Dayak, Madura, Jawa and Banjar tribes. Data analysis uses one way Anava analysis. 15 isolates of microflora were found in Tempoyak which affected its taste and organoleptics, 8 isolates including lactic acid bacteria, including Pediococcus acidilactici, Lactobacillus curvatus, Leuconostoc mesenteroides, Lactobacillus plantarum, Streptococcus thermophiles, Lactobacillus pentosus, Enterococcus faecium, and Weissella paramesenteroides. 1 isolate of non-lactic acid bacteria that is Micrococcus varians. 2 isolates from the yeast group consisted of Saccharomyces cereviciae and Kluyveromyces marxianus, and 4 isolates included Rhizopus, Monilia sitophila, and Mucor roxii. All microflora found were capable of producing lactic acid, except Aspergillus repens. The statistical test results show the significance of its influence on the organoleptic quality of all sub-variables, including color, taste, aroma and texture of Tempoyak. The exploration results in this research indicate opportunities for Tempoyak to be more developed, innovative and contemporary, so that it is preferred by the millennial generation.
Analisis Cluster: Pedagogy Knowledge Guru Menuju Digitalisasi Pendidikan di Era Merdeka Belajar Berdasarkan Daerah Afirmasi Ratnaya, I Gede; Hajiriah, Titi Laily; Fajri, Siti Rabiatul; Fitriani, Herdiyana
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.10750

Abstract

The competence possessed by teachers is an important factor in achieving learning and education goals in schools. One of the essential competencies in the learning process is pedagogical competence. The Era of Independent Learning marks a paradigm shift in education in Indonesia by utilizing technology to improve the quality of learning. However, challenges arise especially in affirmation areas that have different socio-economic conditions and educational infrastructure. This study aims to determine the ability of teachers' pedagogic competence in facing the digitalization of education in the era of Independent Learning based on affirmation areas. In addition, this study also groups teachers based on their pedagogic abilities towards the digitalization of education in the era of Freedom of Learning using cluster analysis analyzed through SPSS For Windows 23. The research sample consisted of 100 respondents from several affirmation regions in Indonesia, such as Bima, West Lombok, East Lombok, Central Lombok, Maluku, NTT, Papua, and Sumbawa. Additional respondents came from non-affirmative areas as comparators, including Mataram City, Surabaya, Kalimantan, and Magetan East Java. The results of the study show that West Lombok Regency has the highest level of pedagogic competence among affirmation areas with a score of 87.74, while Bima Regency has the lowest score of 75. This average score is still categorized as high because it is above 75. The comparison area shows that Magetan has the highest average score of 100. Cluster analysis showed the formation of two groups, namely cluster 1 consisting of teachers from Magetan, Papua, West Lombok, and NTT, and cluster 2 consisting of teachers from Kalimantan, Bima, Surabaya, Mataram City, Maluku, Sumbawa, and Central Lombok.
Colocasia esculenta L. Extract Increase Growth and Motility of Rotenone Induced Zebrafish (Danio rerio) Larvae Stunting Model Furqon, Muhammad; Kumala, Shirly; Farida, Yunahara; Zaidan, Sarah; Sumiyati, Yati
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.10670

Abstract

This study aims to determine the effect of Colocasia esculenta L on the growth and motility of zebrafish (Danio rerio) larvae after induction of rotenone as a stunting model. The Zebrafish have divided 3 groups i.e Normal (embryonic medium without rotenone induction), Negative Control (embryonic medium with rotenone induction), and Treatment Groups (embryonic medium with rotenone induction and Colocasia esculenta extracts, dose 30-60-90 ppm). The stunting model is designed by induction of 12.5 ppb rotenone to Zebrafish larvae. The growth of the larvae was observed by using a microscope to measure the body and head length. Evaluation of motility was carried out by recording the movement of the fish for 1 minute, and the pace was analyzed by using Video Tracker. The evaluations were observed at 3, 6, 9 dpf (day post-fertilization). Colocasia Esculenta Extract (CEE) groups have higher body lengths compared to the Negative Control Group. The lengths were 125.4 µm for CEE30, 119.4 µm for CEE60, and 121.4 µm CEE90 while the length for the Negative Control Group was 118.3 µm at 9 dpf. However, there were no different values for the ratio of head length to body length of all groups. The ratio was 1:6 at 3 dpf, and 1:4 at 6 and 9 dpf. Moreover, the motility was higher in CEE groups compared to the Negative Control at 3, 6, 9 dpf. The body length and motility of Zebrafish larvae higher compare to the Negative Control Group showing the potential of Colocasia esculenta extract on growth and enhancement of motoric activity.
Pengaruh Model Project Based Learning terhadap Aktivitas dan Hasil Belajar Peserta Didik Kelas X SMA Anugrahsari, Iin; Adnan, Adnan; Junda, Muhammad
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.10901

Abstract

The purpose of this study was to investigate the effect of project-based learning models on students' learning activities and outcomes. This research employed a quasi-experimental design with a pretest-posttest control group. The study was conducted from July to August 2022 at SMA Negeri 12 Makassar. The population consisted of 10th-grade students in the science program, totaling 7 classes. Random sampling selected X MIA 4 as the experimental group and X MIA 2 as the control group. Observational sheets were used to monitor student activities, while test instruments were used to assess student learning outcomes. Data collected were analyzed using descriptive statistics and inferential statistical analysis through Statistical Package for Social Science (SPSS) Version 23.0. Analysis of pretest and posttest scores from the experimental group, comprising 34 students, revealed that 5 students achieved a score in the 'very good' category and 9 students in the 'good' category. In contrast, the control group did not have any students in the 'very good' category; only 3 students were in the 'good' category. Observational results from three observers in the experimental group indicated that 96.73% of students were 'highly active', whereas in the control group, 67.07% were 'sufficiently active'. In conclusion, the research findings suggest that the project-based learning model influences students' learning activities and outcomes.
Studi In-Vivo Antifertilitas Stigmasterol Daun Beluntas (Pluchea indica L.): Pengamatan Konsentrasi dan Motilitas Spermatozoa Fatah, M. Arya; Susetyarini, Eko; Waluyo, Lud; Nurrohman, Endrik
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.11295

Abstract

Low public participation in contraception is one of the factors contributing high population density in Indonesia. Indonesia is known as a paradise for medicinal plants, there are many herbs that have the potential to be developed as contraceptives, one of which is the beluntas plant. This study aims to determine the effect of administering various doses of beluntas leaf stigmasterol on the concentration and motility of spermatozoa. The research was conducted at the Biomedical Laboratory, Muhammadiyah University of Malang, Campus 2 for 45 days. This type of research uses true experimental research with a RAL experimental design, consisting of four treatments and six replications. The doses for each treatment are P1 (0.25mg/gbb), P2 (0.5mg/gbb), P3 (0.75mg/gbb) and control (water). The results showed that administering the entire dose of stigmasterol had a significant effect on the concentration and motility of spermatozoa (P<0.05). Spermatozoa concentration P1 (11.83 ± 1.835), P2 (8.17 ± 1.472), P3 (6.33 ± 1.033), control (21.83 ± 3.312). Spermatozoa motility P1 (32.23 ± 25.406), P2 (19.85 ± 7.173), P3 (17.16 ± 2.801), control 975.23 ± 14.417). Stigmasterol from beluntas leaves has potential as a natural antifertility agent in men.
Analisis Kesulitan Guru Biologi dalam Mengembangkan Modul Ajar pada Kurikulum Merdeka di Sekolah Penggerak Kabupaten Temanggung Darmawan, Ericka; Rachman, Fikri; Sukmawati, Ika
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.9982

Abstract

The new policies included in the independent curriculum certainly have several different aspects. One of the differences here is in the teaching tools. In the independent curriculum, the Learning Implementation Plan (RPP) is referred to as a teaching module. Teachers can choose or even modify their own teaching modules that have been provided by the central government. Teachers need to develop teaching modules, but in reality there are still many teachers who experience difficulties in developing teaching modules. This research aims to determine the difficulties experienced by high school biology teachers in developing teaching modules. This study used qualitative research methods. The research subjects taken were the population of class X and XI Biology teachers at SMA Negeri 2 Temanggung, SMA Negeri 1 Candiroto, and SMA Negeri 1 Pringsurat. Data collection techniques used in research include observation, interviews and questionnaires. The research results show that the difficulty of Biology teachers in developing teaching modules is 45%. The level of difficulty is found in indicators of analysis and mapping of student needs, determining the dimensions of the Pancasila student profile, clarity of learning objectives, organizing teaching materials, determining learning models, determining and carrying out assessments, evaluating learning and following up on teaching modules, as well as difficulties in analyzing learning outcomes. will be reduced to a teaching module. The factors that make it difficult for Biology teachers to develop teaching modules are: 1) the number of students; 2) difficulty reading Learning Outcomes (CP); 3) the material is very short and unsystematic; 4) time constraints; 5) minimal reference for the implementation of the assessment; and 6) level of technological mastery. Thus, it can be concluded that the difficulties of high school biology teachers in developing teaching modules in the independent curriculum at Temanggung driving schools are in the "sufficient" category.

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