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Jurnal Kedokteran Hewan

JKH
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Published by Universitas Syiah Kuala

ISSN : 1978225X EISSN : 25025600 DOI : 10.21157

Core Subject : Health, Science,

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

Jurnal Kedokteran Hewan (J. Kedokt. Hewan), or Indonesian Journal of Veterinary Sciences is a scientific journal field of veterinary sciences published since 2007, published FOUR times a year in March, June, September, and December by Universitas Syiah Kuala (Syiah Kuala University) and Indonesian Veterinary Medical Association (PDHI). Jurnal Kedokteran Hewan is a double-blind review process journal that has been accredited by National Journal Accreditation (ARJUNA), with second grade (Sinta 2), Number: 200 / M / KPTS / 2020. This journal has been registered in the Indonesian Publication Index (IPI), Google Scholar, Sinta, World Cat, Directory of Open Access Journals (DOAJ), EBSCO, Copernicus, Microsoft Academic, and other scientific databases. Jurnal Kedokteran Hewan receives scientific manuscripts in veterinary sciences (veterinary miscellaneous): anatomy, histology, physiology, pharmacology, parasitology, microbiology, epidemiology, veterinary public health, pathology, reproduction, clinic veterinary, aquatic animal disease, animal science, and biotechnology.

Arjuna Subject : Kedokteran Hewan - Kedokteran Hewan (Lain-Lain)

Articles 901 Documents

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KEMAMPUAN ANTI MAYOR PHYSIOLOGICAL PROTEIN SUBSTRAT ECTO CYCLIC AMP INDEPENDENT SERIN/THEONIN PROTEIN KINASE (MPS ecto-CIK) DALAM MENGHAMBAT VIABILITAS SPERMATOZOA KAMBING DAN SAPI Bayyinatul Muchtaromah; Sutiman B. Sumitro
Jurnal Kedokteran Hewan Vol 5, No 1 (2011): March
Publisher : Universitas Syiah Kuala

Penelitian ini bertujuan mengetahui pengaruh dosis dan lama inkubasi anti MPS ecto-CIK dalam menghambat viabilitas spermatozoa kambing dan sapi. Rancangan penelitian yang digunakan adalah rancangan acak lengkap (RAL) dengan pola faktorial yang terdiri atas 2 faktor yakni dosis pengenceran (0, 5, 10, dan 15 µl) dan lama inkubasi (5, 30, 60, dan 120 menit) masing-masing 6 kali ulangan. Data yang diperoleh dianalisis dengan analisis varian dua arah, yang dilanjutkan dengan uji jarak Duncan dengan taraf signifikansi 5%. Pemberian anti MPS ecto-CIK membran spermatozoa kambing dengan konsentrasi 0, 5, 10, dan 15 µl dan lama inkubasi 5, 30, 60, dan 120 menit berpengaruh signifikan terhadap viabilitas spermatozoa kambing dan sapi (P0,05). Perlakuan anti MPS ecto-CIK pada dosis 15 µl dan lama inkubasi 120 menit terhadap spermatozoa kambing dan sapi merupakan perlakuan yang paling optimal dalam menghambat viabilitas spermatozoa kambing (45,50±11,16 dan 44,87±9,40%) dan sapi (39,08±14,40 dan 36,67±11,93%).

ISOLASI DAN IDENTIFIKASI VIRUS AVIAN INFLUENZA PADA BERBAGAI SPESIES UNGGAS SECARA SEROLOGIS DAN MOLEKULER (Isolation and Identification of Avian Influenza in Different Species of Poultry by Means of Serological and Molecular Methods) Teuku Zahrial Helmi; Charles Rangga Tabbu; Wayan Tunas Artama; Aris Haryanto; Muhammad Isa
Jurnal Kedokteran Hewan Vol 10, No 1 (2016): March
Publisher : Universitas Syiah Kuala

The purpose of this research was to identify avian influenza (AI) virus using serological and molecular methods on poultry which suspected as AI infected in Aceh province. This study used 37 samples of tracheal and cloacal swabs and organs from various species of poultry that were collected from several districts/cities in Aceh. Samples were collected and put into transport media and stored at 4° C before sending to the laboratory. Samples were inoculated in specific pathogen-free of embryonated chicken egg with the age of 9-11 days for further serological and molecular examination. From 37 samples which infected to embryonated chicken egg then followed by hemagglutinin agglutination test/hemagglutinin inhibition revealed that 7 samples were positively infected with AI virus. The amplification result of specific matrix gene primer was followed by electrophoresis on 2% agarose gel which were obtained in the form of a deoxyribonucleic acid (DNA) band at 276 bp for matrix gene and 1.725 bp for H5 gene for all isolates test. In conclusion, the virus which caused the death of various types of poultry in Aceh province is avian influenza A virus subtype H5.Key words: avian influenza virus, H5N1, serologic, matrix, heamaglutinin

PROFIL DARAH TIKUS PUTIH (Rattus norvegicus) YANG DIINFEKSIKAN Trypanosoma evansi DAN DIBERIKAN EKSTRAK KULIT BATANG JALOH (Salix tetrasperma Roxb) Yudha Fahrimal; Eliawardani E; Afira Rafina; Al Azhar; Nuzul Asmilia
Jurnal Kedokteran Hewan Vol 8, No 2 (2014): September
Publisher : Universitas Syiah Kuala

Penelitian ini bertujuan mengetahui gambaran darah (hematokrit, eritrosit, leukosit, dan diferensial leukosit) tikus yang diinfeksi Trypanosoma evansi (T. evansi) dan diberi ekstrak kulit batang jaloh (Salix tetrasperma Roxb). Duapuluh lima ekor tikus jantan dibagi secara acak ke dalam 5 kelompok perlakuan yang masing-masing terdiri atas 5 ekor tikus. Kelompok 0 (K0) tanpa perlakuan, kelompok I (K1) hanya diinfeksikan dengan 103 T. evansi, kelompok II (K2) diinfeksikan dengan 103T. evansi dan diberikan ekstrak kulit batang jaloh 30 mg/kg bobot badan, kelompok III (K3) diinfeksikan 103 T. evansi dan diberikan ekstrak kulit batang jaloh 45 mg/kg bobot badan, dan kelompok IV (K4) diinfeksi dengan 103 T. evansi dan diberikan ekstrak kulit batang jaloh 60 mg/kg bobot badan. Infeksi T. evansi dilakukan secara intraperitoneal sedangkan ekstrak diberikan secara oral selama 3 hari berturut-turut. Hasil penelitian menunjukkan bahwa rata-rata ± SD nilai hematokrit dan eritrosit tikus dari K1, K2, K3 dan K4 lebih rendah dari K0. Sebaliknya, rata-rata ± SD jumlah leukosit (103/µl) lebih tinggi dari K0. Diferensial leukosit menunjukkan jumlah masing-masing sel leukosit semua tikus dalam kelompok perlakuan meningkat setelah pemberian ekstrak kulit batang jaloh kecuali eosinofil dan limfosit yang justru menurun. Dari penelitian dapat disimpulkan bahwa Infeksi T. evansi menurunkan kadar hematokrit dan eritrosit namun meningkatkan kadar leukosit tikus dan pemberian ekstrak kulit batang jaloh dosis rendah dalam waktu yang singkat mampu mengembalikan profil darah tikus mendekati nilai normal.

THE INFLUENCE OF NUTRITIONAL FLUSHING ON BODY MORPHOMETRICS OF FEMALE BREEDING GOATS Nurul Isnaini; Dedes Amertaningtyas; Hanief Eko Sulistyo; Artharini Irsyammawati; Faizal Andri
Jurnal Kedokteran Hewan Vol 16, No 2 (2022): June
Publisher : Universitas Syiah Kuala

The purpose of this current study was to evaluate the influence of nutritional flushing on body morphometrics of female breeding goats. This study used 32 local female goats or does with an average age of 3.28±1.08 years and an average body weight of 42.47±8.28 kg. The goats were distributed in a completely randomized design using a 2 x 2 factorial pattern with 8 replications. The first factor was the flushing feed (FF) type, consisting of rice bran and concentrate, whereas the second factor was the level of tannin-protected arginine (TPA: 0 and 300 mg/kg). The body morphometrics observed in this study were initial body length (IBL), final body length (FBL), body length change (BLC), initial chest girth (ICG), final chest girth (FCG), chest girth change (CGC), initial wither height (IWH), final wither height (FWH), and wither height change (WHC). The IBL, FBL, BLC, ICG, FCG, IWH, FWH, and WHC of the local does were not significantly different (P0.05) after receiving the FF type. However, FF significantly affected the CGC of the goats (P0.001). The use of concentrate for the local female local goats resulted in a higher CGC (P0.001) compared to the use of rice bran. On the other hand, TPA did not significantly affect all body morphometrics of the goats (P0.05). Thus, it can be concluded that the use of concentrate leads to higher chest girth change, giving better body growth compared to the use of rice bran. The addition of tannin-protected arginine was also found to have no significant contribution to the body morphometrics of the local female goats. _____________

CRYOPRESERVATION OF KAMPUNG ROOSTER SEMEN USING EGG YOLK DILUENT FROM FOUR TYPES OF POULTRY WITH DIFFERENT CONCENTRATIONS Khairuddin Khairuddin; Muhammad Erik Kurniawan; Soman Soman
Jurnal Kedokteran Hewan Vol 13, No 3 (2019): September
Publisher : Universitas Syiah Kuala

The aim of this study was to determine the type and the best concentration of egg yolk in maintaining the quality of kampung rooster spermatozoa during cryopreservation. This study used a completely randomized factorial pattern design with the first factor was the type of egg yolk (purebred chicken, kampung chicken, duck, and quail) and the second factor was the concentration of egg yolk (5%, 10%, and 15%). Semen was collected from twelve kampung roosters using massage method. Immediately after collection, the semen was evaluated macroscopically and microscopically. Semen with more than 70% motility was used in this study. The semen was diluted, packed in a ministraw, equilibrated, and frozen using liquid nitrogen vapor and stored in a liquid nitrogen container for 24 hours. Observation of spermatozoa motility was carried out in fresh semen, diluted semen, after equilibration and after thawing with four replications. The results showed that the type of egg yolk treatment had no effect (P0.05) on the recovery rate and motility of spermatozoa before and after cryopreservation, but egg yolk concentration had a highly significant effect (P0.01) on the quality of spermatozoa. Egg yolks in 10-15% concentration had spermatozoa motility and recovery rate higher than egg yolk with 5% concentration. In conclusion, purebred chicken egg yolk, kampung chicken egg yolk, duck egg yolk, and quail egg yolk each in diluent can be used to maintain the quality of kampung rooster spermatozoa at a concentration of 10-15% during cryopreservation.

REPEATED FREEZE-THAW CYCLES BUT NOT SHORT-TERM STORAGE OF FECAL EXTRACTS AT AMBIENT TEMPERATURE INFLUENCE THE STABILITY OF STEROID METABOLITE LEVELS IN CRESTED MACAQUES Gholib Gholib; Muhammad Agil; Iman Supriatna; Bambang Purwantara; Michael Heistermann; Antje Engelhardt
Jurnal Kedokteran Hewan Vol 11, No 2 (2017): June
Publisher : Universitas Syiah Kuala

The objective of this study was to examine the effects of repeated freeze-thaw cycles and short-term storage of fecal extracts at ambient temperature on the stability of fecal glucocorticoid (fGCM) and estrogen metabolite (fEM) levels from crested macaques.In total 100 aliquots of fecal extracts from fecal samples collected from female crested macaques (Macaca nigra) living at the Tangkoko-Batuangus Nature Reserve, North Sulawesi were used. We performed two different experiments: (1) An experiment to investigate if levels of fGCM and fEM measured from fecal extracts that were exposed to two, four, six and eight repeated freeze-thaw cycles (test groups) differ to control samples (i.e. fecal extracts always stored frozen); (2) An experiment to evaluate whether storing fecal extracts at ambient temperature for two, four, six, and eight days (test groups)affects the levels of fGCM and fEM compared to the control group (i.e. fecal extracts frozen immediately).Results showed that hormone levels were significantly increased (P0.05) after four freeze-thaw cycles for fGCM and after eight freeze-thaw cycles for fEM. By contrast, there was no significant difference (P0.05) in levels of fGCM and fEM between the test groups and the control group in fecal extracts stored at ambient temperature. In conclusion, our data show that more than two and six repeated freeze-thaw cycles should be avoided when measuring fGCM and fEM in crested macaque fecal extracts, respectively. We also demonstrate that storing fecal extracts at ambient temperature is possible for at least 8 days without taking a risk of affecting the stability of fGCM and fEM levels.

PERBANDINGAN INTENSITAS BERAHI SAPI ACEH YANG DISINKRONISASI DENGAN PROSTAGLANDIN F2 ALFA DAN BERAHI ALAMI Hafizuddin Hafizuddin; Tongku Nizwan Siregar; Muslim Akmal; Juli Melia; Husnur rizal; Teuku Armansyah
Jurnal Kedokteran Hewan Vol 6, No 2 (2012): September
Publisher : Universitas Syiah Kuala

Penelitian ini bertujuan mengetahui perbedaan intensitas berahi sapi aceh antara yang disinkronisasi berahi dengan prostaglandin F2 alfa (PGF2α) dan berahi alami. Dalam penelitian ini digunakan 20 ekor sapi aceh betina yang dibagi atas dua kelompok. Kriteria sapi yang digunakan adalah umur 5-8 tahun, mempunyai bobot badan 150-250 kg, dan mempunyai minimal dua siklus reguler. Sapi yang digunakan mempunyai skor kondisi tubuh dengan kriteria baik, yaitu 3 atau 4 pada skala skor 5. Pada Kelompok I (KI) sapi disinkronisasi berahi mengunakan PGF2α sebanyak 5 mg/ml secara intramuskular. Pada kelompok II (KII) sapi dibiarkan memperlihatkan gejala berahi alami. Penilaian intensitas berahi dilakukan dengan memberi skor 1, 2, dan 3, berdasarkan kriteria yang dibuat oleh Kune dan Solihati (2007). Hasil penelitian menunjukkan tidak ada perbedaan intensitas berahi sapi aceh baik yang disinkronisasi berahi dengan PGF2α dan sapi yang mengalami berahi alami dengan skor intensitas berahi masing-masing adalah 2,40±0,84 dan 2,70±0,48.

The Efficacy of Seminal Vesicles Extract Administration on Percentage of Estrus and Pregnancy on Local Goat Syafruddin S; Tongku Nizwan Siregar; Herrialfian H; T Armansyah; Arman Sayuti; Roslizawaty R
Jurnal Kedokteran Hewan Vol 4, No 2 (2010): September
Publisher : Universitas Syiah Kuala

The aim of this research was to determine the percentage of estrus, performance of estrus, and percentage of pregnancy in local goat which synchronized with seminal vesicles extract. The seminal vesicles used in this research were collected from the waste of Banda Aceh slaughter house. The goats were allotted into 2 groups. Goats in group were injected with 0.5 ml PGF2á intramuscularly, and goats in group II were injected with 5 ml seminal vesicles extract intrauterine. The injection was done twice with the interval of 11 days. The goats which perform estrus sign are mated naturally once in every observation. Pregnancy diagnosing was done using chemical urine method 2 months after mating. The estrus percentage and pregnancy data were analyzed with chi-square and the estrus performance (onset and estrus duration) were analyzed using student T test. The estrus onset of group I and II were 29.33±4.62 and 24.00±0.00 hours (P0.05). Estrus duration of group I and II were 26.67+4.62 and 20.00+11.97 hours respectively (P0.05). The estrus percentage of group I and II did not show any significant differences (P0.05), those are 60.0 and 40.0% respectively, whereas the percentage of estrus from both groups were 100.0 %.

KEMAMPUAN OOSIT IKAN LELE (Clarias grapienus) DALAM MENOLERANSI KLORIN SEBAGAI BAHAN OKSIDATIF STRES Hadid Armansyah; Yulianus Linggi; Marsoedi M
Jurnal Kedokteran Hewan Vol 8, No 1 (2014): March
Publisher : Universitas Syiah Kuala

Penelitian ini menggunakan klorin dalam bentuk natrium hipoklorit yang dipaparkan secara in vitro untuk menguji kemampuan oosit ikan lele dalam menoleransi kehadiran klorin sebagai bahan oksidatif stres dalam medium kultur. Konsentrasi klorin yang digunakan adalah 0, 10, 20, 40, 80, 160, dan 320 μM. Hasil penelitian menunjukkan bahwa semakin tinggi konsentrasi paparan semakin rendah jumlah oosit yang mampu bertahan hidup hingga 4 jam pengamatan. Konsentrasi klorin 320 μM menghasilkan jumlah oosit yang mampu bertahan hidup paling optimum. Disimpulkan bahwa oosit ikan lele mampu menoleransi kehadiran bahan oksidatif stres dalam medium kultur dengan konsentrasi paparan klorin sebanyak 320 μM.

COMPARISON OF POLYPEPTIDE PROFILE OF Trypanosoma evansi ISOLATES FROM INDONESIA AND THEIR RELATION TO BIOTYPE AND SENSITIVITY TO TRYPANOCIDAL Ichwan Yuniarto; Didik T Subekti; Umi Cahyaningsih; Fadjar Satrija
Jurnal Kedokteran Hewan Vol 12, No 2 (2018): June
Publisher : Universitas Syiah Kuala

This study aimed to determine whether the variant or biotype of Trypanosoma evansi can be seen from their polypeptide profiles using 12%sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE) stained with Brilliant Blue Commasie. The results generally showed thatthe molecular weight (MW) of polypeptides from nine isolates from East Java, Central Java, Banten, South Kalimantan, Central Kalimantan, andLampung provinces were in the range of 85.46 to 15.76 kD and each isolate has different polypeptide profile. Isolates A13 and A14 were isolatedfrom the same place but have different polypeptide profiles. Likewise, isolates S13 and S18 also have different polypeptide profiles despite beingisolated from the same place at the same time. On the other hand, isolate 372, 87, and 06 have different protein profiles but was classified in thesame biotype namely biotype I. Generally, the difference in protein profile actually more related to the biological diversity of the metabolism ofeach Trypanosoma evansi isolate from Indonesia.

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Tongku Nizwan Siregar

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Jl. Teungku Hasan Krueng Kalee No. 4, Kampus FKH Unsyiah, Kopelma Darussalam, Banda Aceh 23111, Indonesia

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Home Page

OAI Link

Editorial Team

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Contact Name Tongku Nizwan Siregar

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Phone +626517551536

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Editorial Address Jl. Teungku Hasan Krueng Kalee No. 4, Kampus FKH Unsyiah, Kopelma Darussalam, Banda Aceh 23111, Indonesia

Location Kab. aceh besar, Aceh INDONESIA

Abstract

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Abstract

Contact Name
Tongku Nizwan Siregar

Contact Email
jkh@usk.ac.id

Phone
+626517551536

Journal Mail Official
jkh@usk.ac.id

Editorial Address
Jl. Teungku Hasan Krueng Kalee No. 4, Kampus FKH Unsyiah, Kopelma Darussalam, Banda Aceh 23111, Indonesia

Location
Kab. aceh besar,

Aceh

INDONESIA