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INDONESIA
Jurnal Kedokteran Hewan
Published by Universitas Syiah Kuala
ISSN : 1978225X     EISSN : 25025600     DOI : 10.21157
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary
Jurnal Kedokteran Hewan (J. Kedokt. Hewan), or Indonesian Journal of Veterinary Sciences is a scientific journal field of veterinary sciences published since 2007, published FOUR times a year in March, June, September, and December by Universitas Syiah Kuala (Syiah Kuala University) and Indonesian Veterinary Medical Association (PDHI). Jurnal Kedokteran Hewan is a double-blind review process journal that has been accredited by National Journal Accreditation (ARJUNA), with second grade (Sinta 2), Number: 200 / M / KPTS / 2020. This journal has been registered in the Indonesian Publication Index (IPI), Google Scholar, Sinta, World Cat, Directory of Open Access Journals (DOAJ), EBSCO, Copernicus, Microsoft Academic, and other scientific databases. Jurnal Kedokteran Hewan receives scientific manuscripts in veterinary sciences (veterinary miscellaneous): anatomy, histology, physiology, pharmacology, parasitology, microbiology, epidemiology, veterinary public health, pathology, reproduction, clinic veterinary, aquatic animal disease, animal science, and biotechnology.
Arjuna Subject : Kedokteran Hewan - Kedokteran Hewan (Lain-Lain)
Articles 901 Documents
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CHARACTERISTIC OF OVARIAN AND ESTRADIOL CONCENTRATIONS IN THE FOLLICULAR FLUID OF SLAUGHTERED ACEH CATTLE Adam, Mulyadi; Gholib, Gholib; Hafizuddin, Hafizuddin; Zamzami, Rumi Sahara; Bahi, Muhammad
Jurnal Kedokteran Hewan Vol 13, No 4 (2019): December
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v13i4.12890

Abstract

The objective of this study was to characterize the morphology of ovary from slaughtered Aceh cow and measuring the concentration of estradiol in the follicular fluid to examine the potential utilizing for in-vitro fertilization (IVF) development in Aceh cattle. In total, 30 pairs of ovaries were collected from 30 Aceh cattle slaughtered at Banda Aceh abattoir. Morphological characteristics of ovaries observed were the size of ovaries, weight of ovaries, number of follicles, the diameter of follicles, corpus luteal (CL), and volume of follicular fluid. The follicular fluid was aspirated using a disposable syringe and analyzed the estradiol concentrations using enzyme-linked immunosorbent assay (ELISA). The follicles were grouped into 3 categories based on the diameter () of follicles: small follicles ( 1 - 5 mm), medium follicles ( 5 - 8 mm), and large follicles ( 8 mm). The morphological characteristic of ovaries varied between right and left ovaries in which right ovaries showed bigger size and weight compared to the left ovaries (P0.05), whereas the number of follicles and follicular fluid did not significantly different (P0.05). The concentrations of estradiol in follicular fluid of large and medium follicles were significantly higher compared to small follicles (P0.05), whereas the concentration of estradiol between large and medium follicles, did not significant different (P0.05). In conclusion, the follicles from abattoir can be used as potential oocytes resources to support IVF development for Aceh cattle in the future. The follicular fluid provided from these follicles can be an alternative for in vitro maturation media of oocytes due to containing high estradiol concentrations.
CORRELATION BETWEEN PROGESTERONE HORMONE CONCENTRATION AND NUMBER OF FETUSES OF ETAWA CROSSBRED GOAT IN DIFFERENT GESTATION PERIOD Syafruddin, Syafruddin; Rizal, Khairul; Rosmaidar, Rosmaidar; Melia, Juli; roslizawaty, roslizawaty; Zuhrawati, Zuhrawati; Rahmi, Erdiansyah; Abrar, Mahdi
Jurnal Kedokteran Hewan Vol 11, No 3 (2017): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v11i3.8687

Abstract

This study aimed to investigate the correlation between progesterone hormone concentration and number of fetus as well as the difference in progesterone hormone concentration from different gestation periods in Etawah crossbred (EC). Five EC goats used in this study were injected with 2 mL of 5.5 mg prostaglandin F2 (PGF2, Capriglandin) intramuscularly, with an interval of 10 days. Goats that showed symptoms of estrus were mated naturally with male goats. Blood samples were taken on the 7th, 14th, 21st, and 75th days of gestation. Progesterone hormone concentration was determined using enzyme linked immunosorbent assay (ELISA). The number of fetus was determined at 35th gestational day using ultrasonography (USG). Four out of 5 goats were found to have 1 fetus and 1 goat had 2 fetuses. The progesterone concentration on the 7th, 14th, 21st, and 75th days of gestation in goats with single fetus were 5.21, 7.78, 11.97, and 18.78 ng/mL, respectively, while in goat with two fetuses were 8.44, 14.53, 16.81, and 22.73 ng/mL, respectively. The correlation (r) between progesterone hormone concentration and number of fetus on the 7th, 14th, 21st, and 75th days of gestation were 0.442, 0.854, 0.592, and 0.757, respectively. It is concluded that progesterone concentrations are highly correlated to the number of fetus in each gestation period in EC goats.
AKURASI METODE OBSERVASI TIDAK KEMBALI BERAHI (NON-RETURN TO ESTRUS) DAN ULTRASONOGRAPHY (USG) UNTUK DIAGNOSIS KEBUNTINGAN KAMBING PERANAKAN ETTAWAH s, Syafruddin; r, Rusli; h, Hamdan; r, Roslizawaty; Rianto, Sapta; Hudaya, Sapto
Jurnal Kedokteran Hewan Vol 6, No 2 (2012): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v6i2.308

Abstract

Tujuan penelitian ini adalah mengetahui akurasi pemeriksaan kebuntingan dengan metode observasi tidak kembali berahi (non-return toestrus) dan ultrasonography (USG) pada kambing peranakan Ettawah (PE). Penelitian ini terdiri atas 2 percobaan. Pada percobaan I, digunakan kambing betina PE sebanyak 24 ekor dan 5 ekor kambing jantan PE sebagai pengusik. Observasi tidak kembali berahi dilakukan 18-24 hari setelah inseminasi buatan secara visual dan dibantu dengan kambing jantan. Kambing yang tidak memperlihatkan gejala berahi pada saat pemeriksaan diberi nilai positif sedang kambing yang memperlihatkan gejala berahi pada saat pemeriksaan diberi nilai negatif. Hasil pemeriksaan ini akan dikonfirmasi dengan pemeriksaan kebuntingan secara ultrasonography (USG). Kambing yang memiliki nilai positif pada observasi tidakkembali berahi tetapi negatif pada pemeriksaan USG diberi nilai positif palsu, sedang kambing yang memiliki nilai negatif pada observasi tidak kembali berahi tetapi positif pada pemeriksaan USG diberi nilai negatif palsu. Akurasi hasil pemeriksaan untuk diagnosis bunting dan tidak bunting masing-masing adalah 93,75 dan 37,50%. Pada percobaan II, digunakan kambing betina PE sebanyak 160 ekor. Pemeriksaan kebuntingan dilakukan menggunakan USG pada waktu yang telah ditetapkan pada kambing yang telah dikawinkan pada waktu yang bervariasi. Estimasi usia kebuntingan ditentukan dengan memperhitungkan lama kebuntingan pada kambing yakni 5 bulan, kemudian diikuti selisih waktu antara kelahiran dengan jadwal pemeriksaan. Hasil positif dari pemeriksaan ditandai dengan bentukan bulat anechoic di sebelah dorsal dari vesika urinaria sedangkan terlihatnya gambaran muskulus mengindikasikan kambing negatif bunting. Hasil pemeriksaan ini akan dikonfirmasi dengan angka kelahiran. Dari hasil penelitian diperoleh akurasi diagnosis bunting adalah 94,3% sedang akurasi diagnosis tidak bunting adalah 100%. Dari 160 ekor kambing yang diperiksa, diperkirakan jumlah kambing yang bunting pada usia 1, 2, dan 3 bulan masing-masing adalah 19, 98, dan 7 ekor kambing.
GAMBARAN INVOLUSI UTERUS KAMBING KACANG (Capra sp.) BERDASARKAN PENGAMATAN DENGAN ULTRASONOGRAFI TRANSKUTANEUS Riady, Ginta; Siregar, Tongku Nizwan; Melia, Juli; H, Hamdan; Ayunanda, Reni
Jurnal Kedokteran Hewan Vol 9, No 1 (2015): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v9i1.2780

Abstract

Penelitian ini bertujuan mengetahui gambaran involusi uterus kambing kacang berdasarkan pengamatan dengan ultrasonografi (USG) transkutaneus. Hewan yang digunakan adalah 1 ekor kambing kacang betina pascapartus dengan status melahirkan normal dan melahirkan satu ekor anak. Kambing diperiksa dalam posisi berbaring (lateral recumbency). Pengamatan involusi uterus dilakukan setiap hari, dimulai dari hari pertama pascapartus sampai tidak ada lagi pengurangan diameter lumen kornua uterus. Pada hari pertama sampai hari ke-7, gambaran karunkula (hypoechoic), lokia (anechoic), lapisan miometrium, dan endometrium (hypoechoic) yang dipisahkan oleh lapisan pembuluh darah (anechoic) terlihat jelas dengan diameter lumen kornua uterus menurun dari 87,6 menjadi 52,8 mm. Pada hari ke-8 diameter lumen 45,4 mm menurun menjadi 38,4 mm pada hari ke-14. Lokia dan lapisan miometrium masih terlihat sedangkan lapisan pembuluh darah dan karunkula sudah tidak terlihat. Pada hari ke-15 diameter lumen 35,5 mm menurun menjadi 19,3 mm pada hari ke-20 dengan lapisan uterus, lokia, lapisan pembuluh darah, dan karunkula sudah tidak terlihat. Ukuran diameter lumen kornua uterus mengalami penurunan setiap hari dan berhenti mengalami penurunan pada hari ke-20 dengan diameter 19,3 mm. Penelitian ini memperlihatkan bahwa proses involusi uterus kambing dapat diamati dengan metode USG transkutaneus.
EFFECTIVENESS OF PROBIOTIC AND DIGESTIVE ENZYMES IN FERMENTED DIET ON GROWTH, FAT AND CHOLESTEROL CONTENTS OF BROILER Yaman, Dr. M Aman
Jurnal Kedokteran Hewan Vol 16, No 4 (2022): December
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v16i4.25146

Abstract

ABSTRACTThe present study aims to determine the effect of fermented diet containing probiotics and digestive enzymes with different feed levels on growth, fat and cholesterol contents of broiler chickens. Evaluating the effectiveness of probiotics (P) and digestive enzymes (DE) in fermented diet on broiler was observed by 4 treatments: P0=control (fermented diet without P and ED) EP), P1(fermented diet contained 0.5% P only)), P2 (fermented diet contained 0.5% DE only), P3 (fermented diet contained 0.5% P and 1% DE) and P4 (fermented diet contained 1% P and 0.5% DE). A hundred DOCs of broiler chickens strain CP 707 were used (unsex) and it designed by a completely randomized design with 5 treatments, 4 replications and each replication consisted of 5 DOCs. The data obtained were analyzed by means of variance and Duncan's multiple range test. The results of present study showed that broiler fed on fermented diet containing P and/or ED with different levels had a significant effect (P0.05) on final body weight, weight gain, carcass weight, fat content on thigh muscle. Broiler fed on fermented diet contained 0.5% DE only (P2) resulted in better growth and weight gain than other treatments. However, fermented diet contained probiotics and digestive enzymes had no effect on fat and cholesterol contents in breast muscle. It was concluded that the addition of digestive enzymes in fermented diet formulated by local raw materials was very effective than probiotics in increasing body weight gain, carcass weight and prevented the increase in fat and cholesterol contents both in breast and thight muscles of broiler chicken.
THE EFFECT OF COGON GRASS ROOT ETHANOL EXTRACT ON REPRODUCTIVE ORGAN AND EPIDIDYMAL SPERM QUALITY OF MOUSE WITH DIFFERENT AGES Sudiman, Jaqueline; Widyastuti, Rini; Lubis, Alkaustariyah; Hartady, Tyagita; Syamsunarno, Mas Rizky Anggun Adipurna; Sumarsono, Sony Heru
Jurnal Kedokteran Hewan Vol 14, No 1 (2020): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v14i1.14969

Abstract

This study aims to determine changes related to epididymal sperm parameters in mice given cogon grass root ethanol extract (CGREE). The experiment was carried out by dividing three groups of mice aged 4-week old (early pubertal), 8-week old (adult), and 24-week old (old) as control and treatment groups. The treatment groups were given 115 mg/kg body weight of CGREE for 14 days. The mice were sacrificed on day 15 to asses reproductive organ indexes and quality of the epididymal sperm. Index of epididymis and ductus deferens indices significantly reduced in the 4-week old treatment group compared to control (0.23 vs 0.33; 0.08 vs 0.16, respectively), and vesical seminal significantly reduced in 8- week old treatment group compared to control (0.76 vs 0.68). The epididymal sperm quality significantly decreased in all treatment groups, but the sperm concentration in early pubertal group showed an increase. These results indicate that CGREE can enhance several semen parameters in prepubertal and adult mice but this effect is not apparent in old mice.
GENETIC VARIATION OF cDNA OF LDLR GENE IN HYPORESPONDER CYNOMOLGUS MACAQUES (Macaca fascicularis) Taher, Achmad; Solihin, Dedy Duryadi; Sulistiyani, Sulistiyani; Sajuthi, Dondin; Astuti, Dewi Apri
Jurnal Kedokteran Hewan Vol 11, No 3 (2017): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v11i3.2939

Abstract

The study aimed to identify genetic variation of cDNA sequence from low density lipoprotein receptor (LDLR) gene of hyporesponder cynomolgus macaques. The animal used in this study was one hyporesponder cynomolgus macaque obtained from selection result in Primate Research Center-Bogor Institute of Agriculture (PSSP IPB). Amplification of cDNA from LDLR gene was performed using polymerase chain reaction (PCR) method with 4 pairs of walking primer. Alignment of amplification result sequence from 4 pairs of walking primer generated target sequence of 2353 bp which located on position 188-2540. Analysis of target sequence alignment on reference sequence in GenBank found 8 single nucleotide polymorphism (SNP), namely c408CT; c.1200CT; c.1497CT; c.1644TC; 1791TC; 1804AG; 2088CT; and 2377GA. Of 8 SNPs, c.1804AG and 2377GA. Two SNP (c.1804AG; dan 2377GA) caused changing of amino acids composition namely p.K602E (lysine glutamate) and p.V793I (valine isoleucine). This result proved the potential use of genetic variation of cDNA sequence from LDLR gene as genetic marker for selection of hyporesponder cynomolgus macaques.
RESPONS HSP-70 DAN KADAR KORTISOL AKIBAT PEMBERIAN KOMBINASI EKSTRAK JALOH DAN KROMIUM PADA AYAM BROILER YANG MENGALAMI CEKAMAN PANAS s, Sugito; Rahmi, Erdiansyah; Isa, Muhammad
Jurnal Kedokteran Hewan Vol 6, No 2 (2012): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v6i2.341

Abstract

Penelitian ini bertujuan mengetahui pengaruh pemberian kombinasi ekstrak jaloh dan kromium terhadap pembentukan heat shock protein-70 (HSP-70) dan kadar kortisol ayam yang mengalami cekaman panas. Dalam penelitian ini digunakan ayam broiler sebanyak 12 ekor yang dibagi atas empat perlakuan: Perlakuan I (EC), cekaman panas + dan kombinasi 1.000 mg ekstrak jaloh dengan 1.000 g kromium per liter air minum, perlakuan II (E), cekaman panas + 1.000 mg ekstrak jaloh per liter air minum, perlakuan III (KD), cekaman panas + 0,0 mg ekstrak jaloh dan 0,0 g kromium, dan Perlakuan IV (KL), tanpa cekaman panas dan 0,0 mg ekstrak jaloh dan 0,0 g kromium. Masing-masing perlakuan terdiri atas tiga ulangan. Metode pemberian cekaman panas dilakukan dengan meningkatkan suhu dalam kandang pada 331 C selama lima jam per hari dalam waktu lima belas hari. Pemberian kombinasi ekstrak jaloh dan kromium dilakukan dengan cara melarutkannya dalam air minum dan diberikan selama dua jam (pemberian pukul 10.00) sebelum suhu di dalam kandang mencapai 331 C. Pengambilan sampel serum dan jaringan paru dilakukan pada hari ke-15 pelaksanaan penelitian (ayam umur 36 hari). Pengambilan sampel darah dilakukan sebelum ayam dipotong dan jaringan organ paru diambil setelah ayam dipotong. Pada sampel serum dilakukan pemeriksaan kortisol dengan enzymelinkedimmunosorbantassay (ELISA) dan deteksi HSP-70 di dalam jaringan paru dilakukan menggunakan metode imunohistokimia. Hasil penelitian ini menunjukkan bahwa pemberian cekaman panas pada suhu 331 C selama lima jam per hari dapat meningkatkan pembentukan HSP-70 dalam paru dan kadar kortisol dalam serum. Pemberian ekstrak jaloh secara tunggal lebih efektif menurunkan jumlah HSP-70 pada jaringan paru dibandingkan jika dikombinasi dengan kromium.
Morphological on the Macroscropic Development of the Stomach in the Long-tailed Macaque (Macaca fascicularis) during Pre Natal and Post Natal Period Rahmi, Erdiansyah
Jurnal Kedokteran Hewan Vol 1, No 1 (2007): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v1i1.3119

Abstract

This research was conducted with aim to reveal the morphological changes on the stomach in thelong-tailed macaque (Macaca fascicularis) (Mf) during prenatal and postnatal development. Stomachtaken from seven fetuses (40, 55, 70, 85, 100, 120, and 150 gestation day) and two newborns (10 and 105day old) animals was used in this study. The stomach was studied at macroscopic for observation of thegeneral structure. Measurement conducted to stomach length, wide of stomach body, greater curvature,lesser curvature of the stomach in each of old age group. Perception result indicate that the stomach of Mf was red bean like in form with short minor kurvatura. These values increased along with the age of fetusand infant. The Lesser curvature very short. Greater curvature relative length (twice improvement) atpost natal (infant) compared to before birth (fetus). The morphological development of the stomachincreased with age, with very rapid further development after birth, which is possibly due to the age of thefetus and in addition, the start of digestive activity and optimalization of stomach function.Keywords: anatomy, digestion, primates
PENGGUNAAN CAIRAN FOLIKEL DALAM MEDIA MATURASI IN VITRO OOSIT KAMBING BLIGON Widayati, Diah Tri; Fatmawati, Devita Hery; Ariesta, Nony; K, Kustono
Jurnal Kedokteran Hewan Vol 8, No 1 (2014): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v8i1.1263

Abstract

Penelitian ini bertujuan mengetahui pengaruh medium maturasi terhadap kompetensi perkembangan in vitro oosit kambing bligon. Ovarium kambing diperoleh dari Rumah Potong Hewan, dibawa ke laboratorium dalam 0,9% NaCl pada suhu 31-34 C. Oosit diaspirasi dari folikel berdiameter 2-6 mm dengan menggunakan syringe 3 ml dan jarum 23 G. Oosit dengan minimal 2 lapisan sel-sel kumulus diproses untuk maturasi in vitro. Oosit dicuci 3 kali dengan dulbeccos phosphate buffered saline (DPBS) dan tissue culture medium 199 (TCM) 199, kemudian didistribusikan ke dalam 50 l medium maturasi yang berbeda, yaitu TCM 199 (tanpa suplementasi),

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