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INDONESIA
Jurnal Kedokteran Hewan
Published by Universitas Syiah Kuala
ISSN : 1978225X     EISSN : 25025600     DOI : 10.21157
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary
Jurnal Kedokteran Hewan (J. Kedokt. Hewan), or Indonesian Journal of Veterinary Sciences is a scientific journal field of veterinary sciences published since 2007, published FOUR times a year in March, June, September, and December by Universitas Syiah Kuala (Syiah Kuala University) and Indonesian Veterinary Medical Association (PDHI). Jurnal Kedokteran Hewan is a double-blind review process journal that has been accredited by National Journal Accreditation (ARJUNA), with second grade (Sinta 2), Number: 200 / M / KPTS / 2020. This journal has been registered in the Indonesian Publication Index (IPI), Google Scholar, Sinta, World Cat, Directory of Open Access Journals (DOAJ), EBSCO, Copernicus, Microsoft Academic, and other scientific databases. Jurnal Kedokteran Hewan receives scientific manuscripts in veterinary sciences (veterinary miscellaneous): anatomy, histology, physiology, pharmacology, parasitology, microbiology, epidemiology, veterinary public health, pathology, reproduction, clinic veterinary, aquatic animal disease, animal science, and biotechnology.
Arjuna Subject : Kedokteran Hewan - Kedokteran Hewan (Lain-Lain)
Articles 901 Documents
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COMPARISON OF DIFFERENT COMMERCIAL SEROLOGY KITS FOR THE DETERMINATION OF SEROPOSITIVE TOXOPLASMOSIS IN CATTLE IN INDONESIA Subekti, Didik T; Valinata, Sisca; Fong, Sulinawati
Jurnal Kedokteran Hewan Vol 15, No 4 (2021): December
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v15i4.21716

Abstract

This research aims to explain the evaluation of the differences in four commercial kits for the detection of serological toxoplasmosis used in Indonesia. The results of the study found that the toxoplasmosis seropositivity determined by the four commercial kits showed a significant difference (P0.05). Seropositive toxoplasmosis obtained using Pastorex, Toxotest, IDScreen, and Toxo Ab were 35.12%, 60.12%, 26.19%, and 10.12% respectively. IDScreen had a good agreement with Toxo Ab (Gwet's AC1= 0.623) and a moderate agreement with Pastorex (Gwet's AC1= 0.494-0.511). Toxotest had a low agreement with three commercial kits (Gwet's AC1= 0.2) but had a moderate agreement with western blotting (WB) and modified agglutination test (MAT) (Gwet's AC1= 0.458-0.557).
THE EFFECT OF MALACCA LEAVES (Phyllantus emblica) ETHANOLIC EXTRACT ON Plasmodium falciparum GROWTH IN VITRO Asmilia, Nuzul; TR, T Armansyah; Aliza, Dwinna; Melia, Juli; Rahmi, Erdiansyah; Daulay, Lingga Surya Maret
Jurnal Kedokteran Hewan Vol 12, No 4 (2018): December
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v12i4.10215

Abstract

The aim of this research was to find out in vitro antiplasmodium activity of Malacca leaves (Phyllantus emblica) ethanolic extract against Plasmodium falciparum growth. In this study, Plasmodium culture contained 5% parasitemia in ring stage was cultured using candle jar method and antiplasmodial activity test was carried out using microculture. The treatments were divided into 7 groups with four repetitions. K1 as negative control group was given Roswell Park Memorial Institute (RPMI), while K2 as positive control group was given artesdiaquine. Groups K3, K4, K5, K6, and K7 group was added with 100 g/mL, 75 g/mL, 50 g/mL, 25 g/mL, and 5 g/mL of Malacca leaves ethanolic extract, respectively. Antiplasmodial activity was determined by inhibition concentration of 50% parasite growth (IC50). The data were analyzed using ANOVA and followed by Duncan test. The average of parasitemia level in group K1, K2, K3, K4, K5, K6, and K7 were 55.2515.62, 8.502.52, 8.503.00, 9.250.95, 9.002.70, 9.792.06, and 10.752.22, respectively. The average of inhibition percentage in group K1, K2; K3; K4; K5; K6; and K7 were 0.000.00%, 84.624.55%; 84.625.43%; 83.261.73%; 83.714,90%; 82.353,73%; and 80.546.83%, respectively (P0.01). The results showed that the administration of malacca leaves ethanolic extract significantly affect (P0.01) the inhibition of Plasmodium growth as compared to group K1 (negative control). Probit analysis reveals the IC50 value was 3.889 g/mL. In conclusion, all doses of malacca leaves ethanolic extract used in this study was able to inhibit Plasmodium falciparum growth with IC50 value was 3.889 g/mL.
KUALITAS SPERMATOZOA HASIL SEXING MENGGUNAKAN PENGENCER ANDROMED DAN CAUDA EPIDIDYMAL PLASMA-2 (CEP-2) DITAMBAH KUNING TELUR 10% Purwoistri, Rita Fitria; Susilawati, Trinil; Rahayu, Sri
Jurnal Kedokteran Hewan Vol 7, No 2 (2013): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v7i2.922

Abstract

Penelitian ini bertujuan mengetahui kualitas spermatozoa setelah sexing dengan gradien albumin (putih telur) menggunakan pengencerandromed dan cauda epididymal plasma-2 (CEP-2) ditambah kuning telur 10%. Pengamatan kualitas spermatozoa meliputi motilitas, viabilitas,abnormalitas, konsentrasi, dan total spermatozoa motil. Gradien albumin dibuat dengan cara mencampur putih telur dengan pengencer andromed atau CEP-2 ditambah kuning telur 10% sehingga menghasilkan konsentrasi putih telur 10, 30, dan 50%. Pengencer andromed menghasilkan motilitas spermatozoa hasil sexing pada lapisan atas yang lebih tinggi daripada pengencer CEP-2 ditambah kuning telur 10%. Pengencer andromed menghasilkan viabilitas, abnormalitas, konsentrasi, dan total spermatozoa motil yang sama seperti pengencer CEP-2 ditambah kuning telur 10%. Pengencer andromed dan CEP-2 ditambah kuning telur 10% dapat mengurangi penurunan kualitas spermatozoa.
UJI TOKSISITAS AKUT EKSTRAK ETANOL DAUN MALAKA (Phyllantus emblica) TERHADAP MENCIT (Mus musculus). (Acute Toxicity Test of Ethanolic Extract of Malaka (Phyllantus emblica) Leaves on Mice (Mus musculus)) TR, T. Armansyah; Indriany, Sudi; Sutriana, Amalia; Rosmaidar, Rosmaidar; Asmilia, Nuzul; Panjaitan, Budianto; Aliza, Dwinna; Hamdan, Hamdan
Jurnal Kedokteran Hewan Vol 10, No 2 (2016): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v10i2.5137

Abstract

ABSTRACT The aim of this research was to asses the acute toxicity of ethanolic extract of malaka leaves using lethal dose 50 (LD50) on mice (Mus musculus). Twenty male mice weighing between 20-30 g were randomly divided into 4 groups (group K1-K4) of 5 mice each. All mice in group K1, K2, K3, and K4 were administered ethanolic extract of malaka leaves with the dose of 2, 4, 8, and 16 g/kg bw, respectively. Single dose of ethanolic extract of malaka leaves were given by oral gavage prior to clinical observation . The observation period was 14 days post administration, for sign of toxicity symptom, weight loss, and mortality. The result showed that no mortality was observed in the experimental animals during this study. Slight reduction of body weight was observed in group K2, K3, and K4, and no toxicity sign was found during fourteen days of observation. The LD50 of ethanolic extract of malaka leaves was higher than 16 g/kg body weight, thus, the substance was practically non toxic substance.
RESPONS SEL EPITEL USUS (CMT-93) TERHADAP NUTRASETIKAL GALOHGOR Roosita, Katrin; R, Rimbawan; Djuwita, Ita; Damanik, M. Rizal; Kusharto, Clara M.
Jurnal Kedokteran Hewan Vol 9, No 2 (2015): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v9i2.2825

Abstract

Penelitian ini bertujuan membandingkan pengaruh nutrasetikal galohgor dalam bentuk serbuk (GS) dan ekstrak (GE) terhadap proliferasi, morfologi, dan ekspresi gen Aldh1a2 pada sel epitel usus (CMT-93). Galohgor serbuk (GS) dibuat dengan menghancurkan semua bahan dandikeringkan menggunakan drum-dryer sedangkan GE dibuat dengan mengeringkan semua bahan dengan oven, digiling, dan dimaserasi dengan etanol selama 3x24 jam. Perlakuan didasarkan pada konsentrasi akhir -karoten yang berasal dari GS dan GE masing-masing sebesar 0,5; 1,5; dan 5,0 M dalam larutan medium Dulbecco's Modified Eagle's medium (DMEM) yang dilengkapi serum (10%). Analisis proliferasimenggunakan asai 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), dan ekspresi gen dianalisis dengan reverse transcriptasepolymerase chain reaction (RT-PCR). Hasil penelitian meunjukkan bahwa GE pada konsentrasi tinggi (5,0 M) secara signifikan (P0,05) dapatmenekan proliferasi dan memengaruhi morfologi sel CMT-93. Beta-karoten dalam GE dan GS memengaruhi ekspresi gen Aldh1a2 pada sel epitel usus CMT-93
The Effect of Centrifugation on Semen Quality of Peranakan Ettawah Goat's Post Thawing Indiah, Indiah; Wahjuningsih, Sri
Jurnal Kedokteran Hewan Vol 4, No 2 (2010): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v4i2.9809

Abstract

The aim of the research was to determine the optimum of centrifugation on the quality of Peranakan Ettawah goat's spermatozoa prepared for in vitro fertilization preparation.The research of material was frozen semen ofPeranakan Ettawah goat produced by Artificial Insemination Center in Singosari with minimum post thawing motility in 40%. The method of the research was an experiment method with the four treatment, i.e: 1000 (P1), 1500 (P2), 2000 rpm (P3) of different centrifugation. The variable observes were spermatozoa motility, spermatozoa viability, and abnormal morphology of spermatozoa. Data obtain was analyzed statistically using Completely Randomize Design and continued with Tukey test. The result showed that on the upper layer of each treatment (P1, P2, and P3) obtained 55,723,55; 69,553,35; and 55,192,72% for spermatozoa motility, 62,995,87; 73,994,36; and 57,399,22% for spermatozoa viability, and 7,193,64; 8,842,65; and 6,403,00% for abnormal morphology of spermatozoa, descriptively. While on the lower layer the result showed 55,593,99; 68,633,88; and57,612,20% for spermatozoa motility, 70,057,47; 77,441,08; and 69,9311,98% for spermatozoa viability, and 10,366,20; 9,553,27; and 8,092,80% for abnormal morphology of spermatozoa, desciptively. It was concluded that centrifugation in 1500 rpm showed the highest motility and viability on the upper layer and lower layer.
MORFOLOGI DAN MORFOMETRI PERTUMBUHAN RANGGAH VELVET MUNCAK JANTAN (Muntiacus muntjak muntjak) Wahyuni, Sri; Agungpriyono, Srihadi; Agil, Muhammad; Yusuf, Tuty Laswardi
Jurnal Kedokteran Hewan Vol 5, No 1 (2011): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v5i1.419

Abstract

Penelitian ini bertujuan menggambarkan morfologi pertumbuhan ranggah velvet (RV) dua ekor muncak jantan (M1 dan M2) yang dipelihara di kandang penelitian secara terpisah. Tanggal lepas ranggah dan hari pertama percabangan RV serta awal pengelupasan kulit velvet diamati dan dicatat. Pengukuran meliputi panjang dan diameter ranggah velvet utama (RVU) dan ranggah velvet cabang (RVC) dexter et sinister, serta durasi pertumbuhan RV. Rataan SD panjang dan diameter maksimum RVU dan RVC muncak M1 adalah 148,251,12 mm; 46,260,68 mm (panjang), 19,212,35 mm; 9,120,21 mm (diameter) dengan durasi pertumbuhan 104 hari; sedangkan muncak M2 berturut-turut adalah: 146,441,58 mm; 46,941,97 mm (panjang); 13,651,27 mm; 8,490,29 mm (diameter) dengan durasi pertumbuhan 98 hari. Tidak terdapat perbedaan tahapan pertumbuhan RVU dan RVC antara muncak M2 dan M1, namun berbeda pada ukuran RV dan durasi pertumbuhannya. Muncak M2 memasuki ranggah keras setelah pengelupasan kulit velvet lebih cepat dibandingkan M1. Dapat disimpulkan bahwa ukuran pertumbuhan RV berkorelasi erat dengan faktor umur, berat badan, dan postur tubuh muncak jantan.
MIKROMORFOLOGI ALAT KELAMIN PRIMER BIAWAK AIR (Varanus salvator bivittatus) JANTAN (Micromorphological Structure of Primary Reproductive Organ of Male Water Monitor Lizard (Varanus salvator bivittatus)) M, Mahfud; Winarto, Adi; Nisa, Chairun
Jurnal Kedokteran Hewan Vol 10, No 1 (2016): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v10i1.3375

Abstract

The study aims to determine the histological structure of the testes, ductus epididymidis and ductus deferens of Varanus salvator bivittatus. The tissue samples were obtained by the histological techniques preparation with thickness section is 3-4 m then stained using hematoxylin-eosin (HE) and Masson's Trichrome (MT). The result showed that testes are covered by tunica vaginalis and tunica albuginea (tunica fibrous and tunica vasculosa). Tubulus seminiferous as a parenchyma are found in lobules of testes which are formed by septum as extension of tunica albuginea. Tubulus seminiferous is composed by layers of germinal epithelium cells consist of spermatogenia, Sertoli cell, spermatocyte and spermatid which is developed further to be spermatozoa in the lumen of tubulus. Among the tubulus seminiferous, there are groups of epithelioid cells called Leydig cells. The end of tubulus seminiferous formed ductus epididymidis which then ended up and known as ductus deferens. Epididymis is covered by dense connective tissue. Ductus epididymidis is divided into three segments: cranial, medial and caudal. It is lined by pseudostratified columnar epithelium which is varied in its thickness, amount and diameter of lumen. Lumen of the ductus deferens was lined by pseudostratified ciliated columnar epithelium and thin muscularis mucosal layer and thick circular smooth muscle were covered externally.Key words: Varanus salvator bivittatus, testes, ductus epididymidis, ductus deferens, micromorphology
PERKEMBANGAN EMBRIO SAPI SETELAH FERTILISASI MENGGUNAKAN METODE INTRACYTOPLASMIC SPERM INJECTION (ICSI) DAN AKTIVASI DENGAN STRONTIUM Gunawan, Muhammad; Fahrudin, Mokhamad; Boediono, Arief
Jurnal Kedokteran Hewan Vol 8, No 2 (2014): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v8i2.2651

Abstract

Penelitian ini bertujuan mengetahui pengaruh kombinasi intracytoplasmic sperm injection (ICSI) dan aktivasi dengan strontium untuk mengetahui perkembangan pronukleus dan perkembangan embrio sampai tahap blastosis. Kombinasi ICSI dan strontium 20 mM meningkatkan perkembangan pronukleus 2-PN mencapai 43,59%. Hasil perkembangan embrio pada perlakuan kombinasi ICSI dan strontium 20 mM mencapai tingkat perkembangan 2-4 sel (50,5%), 8-16 sel (43,73%), dan blastosis (15,63%). Kesimpulan dari hasil penelitian ini adalah kombinasi ICSI dengan aktivasi strontium 20 mM mampu menghasilkan perkembangan in vitro embrio sapi yang lebih baik.
BODY WEIGHT, MUSCLE WIGHT, PROTEIN, DNA AND RNA CONTENTS IN BREAST MUSCLE (M. Pectoralis Major) OF SELECTED LOCAL MEAT CHICKEN FED ON A DIFFERENT LEVEL OF KIAPU (Pistia stratiotes L.) IN FERMENTED DIET Yaman, M. Aman; Jeksi, Sri; Daud, Muhammad
Jurnal Kedokteran Hewan Vol 16, No 3 (2022): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v16i3.25282

Abstract

The study to evaluate changes in body weight, muscle weight, muscle protein content and total DNA and RNA on breast muscle of selectedlocal chicken due to different levels of fermented feed containing Kiapu (Pistia stratiotes L.) was conducted using a factorial randomized designfor 7 weeks. This study used 72 selected local chickens, which were separated into 2 groups (male and female) and fed on 3 types of feedtreatment, namely P0 (control treatment with 100% commercial feed), P1 (80% commercial feed + 20% fermented Kiapu feed), and P2 (70%commercial feed + 30% fermented Kiapu feed). Parameters were body weight, muscle weight, muscle protein content and total DNA and RNAon breast muscle of chicken. Total protein, DNA and RNA content were analysed by enzymatic reactions of prokaryotic cell and measured with aUV-Spectrophotometer according to the specified OD (optical density). The results showed that feeding Kiapu fermented 20-30% increased bodyweight and protein content of breast muscle of female chicken by change in DNA content. However, the feeding treatment had no changes inmuscle weight and RNA content of both in male and female chicken. On the other hand, substitution of 20-30% Kiapu fermented feed causednegative changes in DNA level of breast muscle. From this study, it was concluded that substitution of fermented feed containing 20-30% Kiapuassociated with the growth process of breast muscle protein is suitable for female but not suitable for male chicken.

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