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Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati
Published by Universitas Atma Jaya Yogyakarta
ISSN : 25273221     EISSN : 2527323X     DOI : doi.org/10.24002/biota
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati merupakan jurnal ilmiah yang memuat hasil-hasil penelitian, kajian-kajian pustaka dan berita-berita terbaru tentang ilmu dan teknologi kehayatian (biologi, bioteknologi dan bidang ilmu yang terkait). Biota terbit pertama kali bulan Juli 1995 dengan ISSN 0853-8670. Biota terbit tiga nomor dalam satu tahun (Februari, Juni, dan Oktober).
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 1,193 Documents
 113   114   115   116   117 
Isolation And Characterization of Lactobacillus Species From Local Indonesian Cow’s Milk Kandou, Abigail Sabrina; Sugata, Marcelia; Jo, Juandy
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 9, No 2 (2024): June 2024
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v9i2.7792

Abstract

Probiotics are a group of beneficial microorganisms that can improve the health of their host. Lactobacillus species are lactic acid bacteria that have great potential as probiotics, in which cow’s milk is a great source of lactic acid bacteria. In this study, Lactobacillus spp. were isolated from fresh local Indonesian cow’s milk. The methods used to characterize the Lactobacillus spp. were (i) biochemical tests including catalase, hemolytic and sugar fermentation tests; (ii) tolerance tests against salt (NaCl 2%, 4% and 6%), low pH (2, 3, 4, 5, 6 and 7) and temperature (7°C, 37°C and 45°C); and (iii) 16S rRNA sequencing. The isolation yielded 14 isolates matching the criteria of Lactobacillus spp. colony and cell (i.e., Gram positive rods that did not produce endospores and did not have a waxy layer covering its cell wall). Based on subsequent biochemical tests, 5 isolates were suspected as potential probiotic Lactobacillus spp. The 16S rRNA sequencing revealed that the isolate L was Limosilactobacillus fermentum. In conclusion, this study demonstrated that local cow’s milk could be used to isolate Lactobacillus spp.
Pattern of Antibiotic Resistance in Salmonella sp. Bacteria Contaminating Fresh Faeces of Laying Hens in Kediri District, West Lombok Regency Ramdani, Fitrah Akbar; Riwu, Katty Hendriana Priscilia; Kholik; Mbura, Yonanda Verawati Haslinda; Zakarias, Herdin Vanek
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 9, No 2 (2024): June 2024
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v9i2.7814

Abstract

Antibiotic resistance has become a common problem in poultry farming in Indonesia. One of them is caused by improper use of antibiotics. This study aims to determine the pattern of resistance of Salmonella sp. isolated from laying hens to antibiotics. The samples used in this study were thirty samples of fresh faeces of laying hens from Kediri District, West Lombok Regency. The samples were isolated and identified through Gram staining and biochemical tests (indole, methyl red-Voges Proskauer, triple sugar iron agar, citrate, and urease), resulting in ten positive samples of Salmonella sp. The study continued with antibiotic resistance testing using disc diffusion or Kirby-Bauer methods. The antibiotics used in the study were aztreonam, ciprofloxacin, streptomycin, chloramphenicol, and tetracycline. The results showed that Salmonella sp. has been resistant to some antibiotics. The highest resistance levels are streptomycin and tetracycline at 50% (5/10), while the lowest is ciprofloxacin at 10% (1/10). Antibiotics that are still sensitive are aztreonam 90% (9/10) and chloramphenicol 100% (10/10). The findings of this study conclude that there is a pattern of antibiotic resistance in laying hens farms that can have a negative impact on human and animal health.
Penambahan Biji Chia (Salvia hispanica L.) pada Fermentasi Tempe Kedelai dalam Peningkatan Aktivitas Antioksidan dan Nilai Kesukaan Dwianto, Stefanus Agung; Meitiniarti, V Irene; Sukmana, Andreas Binar Aji; Dewi, Lusiawati
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 9, No 2 (2024): June 2024
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v9i2.7828

Abstract

Tempe merupakan produk pangan berbahan kedelai (Glycine max) yang difermentasi dengan melibatkan mikroba dari genus Rhizopus. Tempe memiliki berbagai kandungan gizi dan senyawa, salah satunya adalah antioksidan. Untuk meningkatkan kandungan antioksidan dalam tempe dapat dilakukan dengan penambahan bahan alami. Biji chia (Salvia hispanica) dipilih karena termasuk bahan pangan fungsional dan memiliki kandungan antioksidan. Tujuan penelitian ini untuk mengetahui efektivitas penambahan biji chia pada fermentasi tempe kedelai dalam peningkatan antioksidan dan nilai kesukaan. Penelitian dilakukan menggunakan RAL dengan satu faktor dan empat taraf perlakuan, yaitu konsentrasi penambahan biji chia 0%,  0,5%, 1% dan 1,5% (b/b). Penentuan aktivitas antioksidan dilakukan dengan metode DPPH, sedangkan uji hedonik dilakukan menggunakan kuesioner pada 15 orang panelis. Hasil pengukuran antioksidan menunjukkan penambahan biji chia pada tempe dapat meningkatkan aktivitas antioksidan yang dapat dilihat pada persen inhibisi. Persen inhibisi paling tinggi yaitu pada konsentrasi biji chia 1% yaitu sebesar 69,83%. Pada uji hedonik dengan uji Univariate dan uji lanjutan Duncan, perlakuan tempe tanpa pemberian bumbu menunjukkan tidak berbeda nyata antar konsentrasi perlakuan pada parameter aroma dan tekstur. Sedangkan perlakuan tempe dengan penambahan bumbu menunjukkan tidak berbeda nyata antar konsentrasi perlakuan pada parameter tekstur dan rasa.
Keanekaragaman Jenis Ikan di Hulu Sungai Opak menggunakan Environmental DNA (eDNA) Metabarcoding Yudha, Donan Satria; Salsabila, Sophia; Priyono, Dwi Sendi
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 9, No 3 (2024): October 2024
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v9i3.7864

Abstract

Penelitian mengenai keanekaragaman jenis ikan di Hulu Sungai Opak DIY sebelumnya telah dilakukan pada tahun 2010 dan 2013 dengan metode konvensional. Akan tetapi, metode tersebut memiliki beberapa keterbatasan. Monitoring menggunakan eDNA metabarcoding diperlukan untuk memperoleh data yang lebih detail dan akurat. Pada penelitian ini, digunakan primer 250 bp 16S Vertebrate. Penelitian ini bertujuan untuk mengetahui keanekaragaman jenis ikan di hulu Sungai Opak pada tahun 2022 dengan pendekatan eDNA metabarcoding; mengetahui perbedaan keanekaragaman jenis ikan di hulu Sungai Opak pada tahun 2010, 2013, dan 2022; dan menguji efektivitas identifikasi keanekaragaman jenis ikan menggunakan eDNA metabarcoding di sungai-sungai di Daerah Istimewa Yogyakarta. Metode yang digunakan dalam penelitian ini meliputi sampling air, filtrasi dan preservasi DNA, ekstraksi DNA, PCR dan elektroforesis, sequencing, dan analisis bioinformatik. Hasil penelitian menunjukkan bahwa teridentifikasi 11 spesies ikan dengan nilai minimum percentage of identical matches sebesar 97%. Jenis ikan yang teridentifikasi pada tahun 2022 lebih banyak dibandingkan pada tahun 2010 dan 2013. Efektivitas penggunaan eDNA metabarcoding dalam identifikasi keanekaragaman jenis ikan di sungai dapat dilakukan secara cepat namun kurang akurat. Ketidakakuratan tersebut disebabkan oleh kontaminasi eDNA, kurangnya spesifitas target sekuens dan primer untuk monitoring ikan, serta database referensi yang belum lengkap untuk ikan air tawar di Indonesia.
Natural Dye as an Alternative to Hematoxylin-Eosin Staining on Histological Preparations Karlina, Ina; Pusparini, Nur Ainun Oktavia; Maharesi, Chesa Ekani; Saeed, Faisal; Retnoaji, Bambang; Saragih, Hendry; Septriani, Nur Indah; Rohmah, Zuliyati; Hadi, Susilo; Nuriliani, Ardaning
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 2 (2025): June 2025
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i2.7909

Abstract

Hematoxylin-eosin is widely utilized in the field of animal microtechniques. However, the need to develop alternative dyes from natural sources such as plants has gained attention. Several studies have shown that many plants contain secondary metabolites with the potential to be developed as natural dyes. Lonchocarpus cyanescens and Syzygium cumini are promising candidates as alternative dyes for hematoxylin, while Lawsonia inermis and Hibiscus sabdariffa have shown potential as substitute dyes for eosin. These plants contain various secondary metabolites, including anthocyanins, flavonoids, chlorophyll, betalains, alkaloids, saponins, tannins, carbohydrates, proteins, phenolics, terpenoids, quinones, coumarins, xanthones, and resins. L. cyanescens exhibits a strong binding affinity to cells and tissues, particularly testicular tissue. Dyes derived from Syzygium cumini have been shown to provide a good staining result for rat liver cells. In contrast, dyes from Lawsonia inermis can stain cytoplasmic components and muscle fibers. Additionally, the dye from Hibiscus sabdariffa is capable of staining various biological components, including sperm, nerve cells, and blood cells. The dye preparation process involved extraction from different plant organs, such as leaves, flowers, and fruit. These findings suggest that secondary metabolites from these four plants hold significant potential for development as natural dyes to replace hematoxylin-eosin in histological applications.
Assessment on the Antimicrobial Activity of Lactiplantibacillus plantarum SU-KC1a against Listeria monocytogenes ATCC-7644 Timothy, Michael; Samantha, Ariela; Sugata, Marcelia; Tan, Tjie Jan; Jo, Juandy
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 1 (2025): February 2025
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i1.8038

Abstract

Lactobacillus species are particularly noteworthy due to their ability to synthesize a variety of antimicrobial substances, including organic acids and bacteriocins. Lactiplantibacillus plantarum SU-KC1a, a member of the genus of Lactobacillus, had been previously isolated from human breast milk by our group. A preliminary in silico investigation had identified that Lpb. plantarum SU-KC1a possessed genes responsible for plantaricin, which is a type of bacteriocin produced by Lpb. plantarum strains. This study therefore aimed to assess the antibacterial activity of Lpb. plantarum SU-KC1a against pathogenic Listeria monocytogenes ATCC-7644 and to induce the biosynthesis of plantaricin by Lpb. plantarum SU-KC1a through co-culture with L. monocytogenes ATCC-7644 as the inducer strain. Our results showed that the cell-free supernatants from both co-culture and monoculture of Lpb. plantarum SU-KC1a exhibited a modest antibacterial activity against L. monocytogenes ATCC-7644. However, it was further determined that the inhibitory effect was not due to the production of plantaricin. Instead, it was primary attributed to the production of organic acids, which decreased the pH and inhibited the growth of L. monocytogenes ATCC-7644. In conclusion, our study demonstrated that Lpb. plantarum SU-KC1a could exert a modest antibacterial activity towards L. monocytogenes ATCC-7644, but not through the induction of plantaricin.
Bentuk Trikoma (Urticaceae) dan Pemanfaatan dalam Pengobatan Tradisional pada Masyarakat Tablasupa Distrik Depapre Kabupaten Jayapura Demena, Widelmina Keterina; Raunsay, Edoward Krisson; Aisoi, Leonardo E.
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 9, No 3 (2024): October 2024
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v9i3.8099

Abstract

Masyarakat Kampung Tablasupa Distrik Depapre menggunakan tumbuhan daun gatal, daun jilat, dan jelatang dalam pengobatan secara tradisional untuk mengatasi keluhan kesehatan. Tujuan dari penelitian ini adalah untuk mengetahui bentuk trikoma Daun Gatal (Laportea decumana (Roxb.) Wedd.), Daun Jilat (Villebrunia rubescens Bl.), Daun Jelatang (Urtica dioica L.) dan penggunaan daun dalam pengobatan tradisional. Metode yang digunakan dalam penelitian ini adalah metode observasi, eksperimen, wawancara, dokumentasi, dan studi pustaka. Analisis data yang digunakan adalah analisis deskriptif kualitatif. Dari hasil penelitian ini diketahui bahwa bentuk trikoma Daun Gatal dan Daun Jilat berbentuk uniseluler sedangkan bentuk trikoma pada Daun Jelatang berbentuk bintang. Daun gatal dapat digunakan untuk menghilangkan rasa pegal-pegal pada badan, Daun Jilat dapat digunakan untuk mengeluarkan darah mati atau darah kotor akibat memar, Daun Jelatang dapat digunakan untuk mengobati asam urat.
Cell Surface and Adherence Properties of Indonesian Indigenous Lactic Acid Bacteria as Probiotic Candidate Leksono, Benediktus Yudo; Purwijantiningsih, Ekawati; Wibisono, Gracia Irene Benaya
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 9, No 1 (2024): February 2024
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v9i1.8124

Abstract

Adherence to intestinal mucosa is one of the crucial probiotic traits. This ability varied among strains. This study aims to evaluate the cell surface properties and adherence potential of Indonesian Indigenous LAB. The adherence potential was evaluated using auto- aggregation, coaggregation against Salmonella, cell hydrophobicity, and adherence to stainless-steel surface. All strains classified as having medium high aggregation (>90%) after 24 h of incubation and can coaggregate with Salmonella (58-92%). Among all strains, Levilactobacillus brevis AB3 showed the highest hydrophobicity (36%) and adhesion to stainless steel (6.37 log CFU/mL). Current findings suggest that Indonesian Indigenous LAB, especially L. brevis AB3, possessed an adherence property and has a potential to be developed into probiotic bacteria.
Canting Expedition: Herpetofauna Diversity in the Mendolo Village, Lebakbarang, Pekalongan Gunawan, Dwi Ariya; Wijayati, Qadriyah Fitri Catur; Rahmajati, Raden Roro Digwikan; Fitriana, Isnaini; Fatmawati, Dwi; Arif , Irvanda Mustofa
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 9, No 3 (2024): October 2024
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v9i3.8131

Abstract

Central Java is an area that has natural forests with quite high biodiversity potential, one of which is Mendolo Village, Lebakbarang, Pekalongan. The diversity of herpetofauna species is one of its potential biodiversity. Data collection on herpetofauna in Mendolo Village has not yet been carried out, even though this village is in direct contact with natural forests with great potential. The aim of this research is to reveal the diversity of herpetofauna types at this location. The method used is VES (Visual Encounter Survey). The data obtained was then analyzed using the Shannon-Wiener Diversity Index. There were 23 species of reptiles and 16 species of amphibians, with a Diversity Index value of 2.942 for reptile types and 2.28 for amphibians. The conclusion that can be drawn is that the diversity of herpetofauna species in Mendolo Village is in the quite high category.
Sinergisme Pertumbuhan dan Tingkat Kesukaan pada Pembuatan Tempe Probiotik dengan Penambahan Pichia kudriavzevii TMT-2 dan Lactobacillus plantarum Dad-13 Yafi , Muhammad Raihan; Yulianto, Wisnu Adi
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 9, No 1 (2024): February 2024
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v9i1.8181

Abstract

Tempe probiotik dapat dibuat dengan penambahan khamir probiotik (Pichia kudriavzevii TMT-2) dan bakteri asam laktat (Lactobacillus plantarum Dad-13). Penelitian ini bertujuan untuk mempelajari sinergisme pertumbuhan antara jamur tempe, khamir dan bakteri probiotik, serta menentukan tempe probiotik yang disukai oleh panelis. Penelitian ini dikerjakan dengan menggunakan rancangan acak lengkap dua faktor, yaitu faktor pertama ialah jenis inokulum (tempe jamur merk Raprima, Raprima + Pichia kudriavzevii TMT-2, Raprima + L. plantarum Dad-13, Raprima + P. kudriavzevii TMT-2 + L. plantarum Dad-13. Faktor kedua adalah lama fermentasi (0, 24, 48, dan 72 jam). Hasil penelitian menunjukkan penambahan yeast, bakteri, dan dan bakteri probiotik tidak menghambat pertumbuhan jamur tempe. Penambahan khamir probiotik mempercepat laju pertumbuhan jamur tempe. Khamir dan bakteri probiotik yang ditambahkan dapat tumbuh dengan baik dan dapat mencapai 9 log10 CFU/g pada akhir fermentasi. Penambahan khamir dan bakteri probiotik tidak menurunkan tingkat kesukaan panelis dibandingkan dengan kontrol (jamur tempe). Berdasarkan jumlah probiotik dan tigkat kesukaan panelis perlakuan terbaik adalah tempe yang diinokulasi dengan Raprima + P. kudriavzevii TMT-2 + L. plantarum Dad-13. Tempe tersebut mengandung jumlah jamur sebanyak 8,53 log10 CFU/g, khamir 9,06 log10 CFU/g, dan bakteri 8,88 log10 CFU/g. Tempe probiotik ini layak untuk dikembangkan khususnya diversifikasi produk olahannya dan tingkat kesukaan konsumen.

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