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Perbedaan Pemberian Kapur Dan Dolomit Terhadap Pertumbuhan Miselium Jamur Tiram Putih (Pleurotus ostreatus (Jacq. Ex. Fr) Kummer) || Differences of Giving Calcite And Dolomite To The Myselium Growth White Oyster Mushroom (Pleurotus ostreatus (Jacq. Ex. Fr) Kummer) Berutu, Mei Awbina; Wibowo, Risky Hadi; Sinisuka, Alfredi Anis Fadhila Ginting; Darwis, Welly; Sipriyadi, Sipriyadi; Berutu, Ali Sadikin
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 6, No 2: Jurnal Pembelajaran Dan Biologi Nukleus September 2020
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v6i2.1799

Abstract

Jamur Tiram putih (Pleurotus Ostreatus (Jacq. Ex. Fr) Kummer) adalah jenis jamur kayu yang memiliki kandungan nutrisi lebih tinggi dibandingkan jenis jamur lainnya dan banyak diminati di Indonesia. Untuk pertumbuhan miselium jamur tiram dan di gunakan kapur dan dolomit. Dolomit dan kapur memiliki kemampuan untuk mempercepat pertumbuhan jamur. Tujuan dari penelitian ini untuk mengetahui perbedaan pemberian dosis kapur dan dolomit yang tepat terhadap pertumbuhan miselium jamur tiram putih. Penelitian ini dilakukan di Usaha Bersama Jamur Tiram Medan, Sumatera Utara dengan menggunakan bibit F2 dari jamur tiram yang diperoleh dari lokasi penelitian. Penelitian ini menggunakan metode eksperimen dengan 2 penelitian dengan penelitian I (yaitu pengaruh perlakuan kapur) dan penelitian II (pengaruh perlakuan dolomit) dengan masing-masing 4 perlakuan dan 6 ulangan. 10 gram kapur dan 30 gram dolomit merupakan dosis yang optimum pada pertumbuhan miselium jamur tiram putih.White Oyster Mushroom (Pleurotus ostreatus (Jacq. Ex. Fr) Kummer) is a type of wood fungus that has a higher nutrient content than other types of mushrooms and is in great demand in Indonesia. For the growth of oyster mushroom mycelium, Calcite and dolomite are used. Dolomite and Calcite have the ability to accelerate mold growth. The purpose of this study was to determine the differences in the correct dose of Calcite and dolomite for the growth of mycelium white oyster mushrooms. This research was conducted at the Medan Oyster Mushroom Joint Venture, North Sumatra using F2 seeds from oyster mushrooms obtained from the research location. This study used an experimental method with 2 studies with research I (namely the effect of lime treatment) and research II (the effect of dolomite treatment) with 4 treatments and 6 replications respectively.10 grams of Calcite and 30 grams of dolomite is the optimum dose for the growth of white oyster mushroom mycelium
Antibacterial Activity of Ethanol Extracts from Leaves and Flowers of Katang-Katang Ipomoea pes-caprae (L) R.Br Against Pseudomonas aeruginosa Wibowo, Risky Hadi; Nabella, Inastia; Fitri, Sabrina; Rahmawati, Suci; Hermansyah, Oky; Masrijal, Camelia Dwi Putri; Slamet, Samwilson; Mashudi, Mashudi; Hidayah, Thoriqul
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 10, No 1: Jurnal Pembelajaran Dan Biologi Nukleus March 2024
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v10i1.4779

Abstract

The resistance character and infection pathogenicity of bacteria to humans is currently increasing, so there is a need for new alternative drugs. The bioactive compounds contained in katang-katang plant are one of the new sources of antibacterials obtained from nature. This study used leaves and flower samples of Katang-katang (Ipomoea pes-caprae (L)R.Br) obtained from the coastal area of Bengkulu City. The purpose of this study was to determine the phytochemical compounds of the leaves and flower extracts of Katang-katang using color reaction test method and to determine the antibacterial activity of the extracts of the leaves and flowers of Katang-katang against the testing pathogenic bacteria (Pseudomonas aeruginosa) at concentrations of 10%,25%,50%,75% using disc diffusion method. The leaves and flowers ethanolic extracts of the Katang - katang were proven contain secondary metabolites such as alkaloids, flavanoids, saponins, tannins and steroids. Antibacterial effectiveness of the ethanol extract from leaves was classified as moderate at a concentration of 50% with a measured inhibition zone of  6.30 mm and a concentration of 75% with an inhibition zone size 8.13 mm. In contrast, effectiveness of the antibacterial extracted from flower was classified as weak at a concentration of 50% with an inhibition zone 4.50mm and moderate at a concentration of 75% with a measured inhibition zone size of 6.40 mm. The experiment revealed that the leaves and flowers of the Katang-katang have antibacterial potential against P. aeruginosa which could be developed as a medicinal ingredient in the future
Perbandingan Struktur Komunitas Cacing Tanah Megadrilli pada Ekosistem Alami dan Ekosistem Buatan Jody, Regina; Darmi, Darmi; Rizwar, Rizwar; Jarulis, Jarulis; Wibowo, Risky Hadi
Bioscientist : Jurnal Ilmiah Biologi Vol. 11 No. 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.9262

Abstract

Earthworms are an important component of soil ecosystems. The presence of earthworms in a habitat can enhance the quality of the soil, both in its physical, chemical, and biological properties. This research has the purpose to analyze the comparison of earthworm density found in natural ecosystems and artificial ecosystems. The determination of location points is done using the Cluster Sampling method, which includes both natural and artificial ecosystems. Sampling is conducted using the line transect method along a 100-meter length in 20 plots. The earthworm collection method involves the use of square and hand sorting methods. Data analysis includes density values, frequency, diversity index, and the Morisita index, as well as statistical analysis using an independent sample T-Test to determine the significance of the difference in earthworm abundance between the two locations. Five species of megadrillic earthworms were identified, classified into two ecological categories: epigeic and endogeic. In the natural ecosystem, there were five species, namely Pheretima capensis (epigeic), P. corethururus (endogeic), Pheretima sp.1 (epigeic), Megascolex sp. (endogeic), and Pheretima sp.2 (epigeic). Meanwhile, in the artificial ecosystem, three species were found, which are P. capensis (epigeic), P. corethururus (endogeic), and Pheretima sp.2 (epigeic). The density of megadrillic earthworms in the natural ecosystem (567.8 individuals/m2) is significantly higher compared to the artificial ecosystem (242.5 individuals/m2). The relative frequency of earthworms in the natural ecosystem is divided into 3 categories: Rare (Assesory), Common (Constant), and Very Common (Absolute), whereas in the artificial ecosystem, there are 2 categories: Common (Constant) and Very Common (Absolute). The diversity index of earthworms in the natural ecosystem tends to be higher (H' = 1.236) than in the artificial ecosystem (H' = 0.885). The distribution pattern of megadrillic earthworms in the natural ecosystem is clustered, while in the artificial ecosystem, it is random.
Aktivitas Antibakteri Ekstrak Daun Mangrove (Rhizophora mucronata) pada Bakteri Helicobacter pylori Penyebab Tukak Lambung Pertiwi, Reza; S, Salprima Yudha; Wibowo, Risky Hadi; Notriawan, Doni; Nasution, Riski Padilah; Azhar, Afra Wafiqah
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.9957

Abstract

Mangrove (Rhizophora mucronata) is a plant that can be used as an antibacterial because it contains several secondary metabolite compounds such as saponins and flavonoids which can inhibit bacterial growth. This research aims to determinehe antibacterial activity of mangrove leaves (Rhizophora mucronata) in inhibiting the growth of Helicobacter pylori bacteria. This research is an in vitro study using the agar dilution method for 2-5 days. The treatment groups consisted of negative, positive (co-trimoxazole), ethanol extract of mangrove leaves (Rhizophora mucronata) with concentrations of6.25%, 12.5%, 25% and 50%. The treatment was carried out by adding the Helicobacter pylori suspension to a petri dish containing agar media. Observations were made to see whether bacteria grew in the media. The data is then analyzed and broken down from the results of calculating the diameter of the inhibition zone. The test results showed that the ethanol extract of mangrove leaves (Rhizophora mucronata) had antibacterial activity in the weak category in inhibiting the growth of Helicobacter pylori bacteria. This antibacterial activity can be caused because mangrove leaves (Rhizophora mucronata) contain secondary metabolite compounds with an antibacterial mechanism of action, so this is in line with the results of this study.
Aktivitas antibateri sediaan hand and body lotion dari ekstrak etanol daun binahong (Anredera cordifolia (Ten.) Steenis) terhadap bakteri Staphylococcus aureus Dominica, Dwi; Anisah, Saskia; Wibowo, Risky Hadi; Shufyani, Fahma
Journal of Pharmaceutical and Sciences Suppl. 1, No. 1 (2023)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v6i5-si.405

Abstract

Background: Formulation is an activity to design the composition of the active ingredients and additional ingredients to make specific preparations. One of the herbal plants that can be formulated in hand and body lotion is binahong leaves (Anredera cordifolia (Ten.) Steenis), which contain secondary metabolites such as flavonoids, saponins, alkaloids, and tannins. These secondary metabolites function as antibacterials. Gram-positive bacteria often cause skin problems, such as Staphylococcus aureus. This study aimed to determine the antibacterial activity of 5%, 7.5%, 10%, 12.5%, and 15% binahong leaf extract hand and body lotion against S. aureus bacteria. This research was conducted experimentally, including an examination of the physical quality of the preparation (organoleptic, homogeneity, pH, spreadability, and adhesion). This Method research is an experimental study, including collecting samples, making kepok banana heart calyx juice with concentrations of 2%, 4% and 6%, ointment evaluation tests and cut wound healing activity tests on white mice which were grouped into five groups with each group consisting of of 5 mice. Statistical analysis included a one-way ANOVA test to see whether there was a significant difference between concentrations and wound healing in white male mice. The results showed that binahong leaf extract could be formulated into hand and body lotion preparations because it has a homogeneous composition, a pH range of 4.8-5.1, spreadability of 5.7-6.0 cm, and adhesion of 2.16-2.49 seconds. Hand and body lotion preparations with 10% binahong leaf extract have the best physical quality. All formulations of binahong leaf extract hand and body lotion in this study could inhibit the growth of S. aureus with weak inhibition, and the best formula for inhibiting the growth of S. aureus was 15% binahong leaf extract hand and body lotion having a diameter of inhibition zone 2.575 m.
Bioprospeksi Senyawa Antibakteri dari Bakteri yang Berasosiasi pada Bulu Babi asal Kabupaten Kaur, Bengkulu Wibowo, Risky Hadi; Sipriyadi, Sipriyadi; Adfa, Morina; Prastya, M. Eka; Cahlia, Uci; Fahmi, Riziq Ilham Nur; Rothman, Dita Delia
Jurnal Kelautan Tropis Vol 28, No 3 (2025): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkt.v28i3.29412

Abstract

The digestive system of sea urchins functions to process organic material from food into bioactive compounds. . However, the direct exploitation of bioactive compounds from sea urchins may trigger ecological imbalances. These bioactive compounds can also be produced through interactions between sea urchins and their diverse associated bacteria within the body. Therefore, alternative methods are needed to obtain bioactive compounds without disrupting the aquatic ecosystem. This study aims to explore bioactive compounds from the associated bacteria of sea urchins (Stomopneustes variolaris Lamarck and Tripneustes ventricosus Lamarck) originating from Kaur Regency, Bengkulu. A total of 30 bacterial isolates were successfully obtained using Zobell Marine Agar (ZMA) medium. Potential isolates were identified through morphological observation, Gram staining, and biochemical tests. Antibacterial activity assays showed that 12 isolates had potential as antibacterial producers based on tests against Bacillus subtilis, Escherichia coli, and Staphylococcus aureus. Two isolates, TVL 6 and TVL 11, were able to inhibit all test bacteria. Additionally, assays using pellets and supernatants showed that all 12 isolates exhibited antibacterial activity, with 5 isolates showing activity in the supernatant. Isolates TVL 6 and TVL 11 were proven effective in inhibiting all test bacteria in both pellet and supernatant forms, with the highest inhibition observed against Staphylococcus aureus. These results indicate that the associated bacteria in the digestive tract of sea urchins have potential as candidates for antibacterial compound producers.   Sistem pencernaan bulu babi berfungsi untuk mengolah bahan organik dari makanan menjadi senyawa bioaktif. Namun, eksploitasi senyawa bioaktif dari bulu babi secara langsung dapat memicu ketidakseimbangan ekosistem. Senyawa bioaktif ini juga dapat dihasilkan melalui interaksi antara bulu babi dengan bakteri asosiasi yang beragam di dalam tubuhnya. Oleh karena itu, diperlukan metode alternatif untuk memperoleh senyawa bioaktif tanpa merusak keseimbangan ekosistem perairan. Penelitian ini bertujuan untuk mengeksplorasi senyawa bioaktif dari bakteri asosiasi bulu babi (Stomopneustes variolaris Lamarck dan Tripneustes ventricosus Lamarck) asal Kabupaten Kaur, Bengkulu. Sebanyak 30 isolat bakteri berhasil diisolasi menggunakan media Zobell Marine Agar (ZMA). Identifikasi isolat potensial dilakukan melalui pengamatan morfologi, pewarnaan Gram, dan uji biokimia. Uji aktivitas antibakteri menunjukkan bahwa 12 isolat memiliki potensi sebagai penghasil antibakteri berdasarkan pengujian terhadap kultur uji Bacillus subtilis, Escherichia coli, dan Staphylococcus aureus. Dua isolat, TVL 6 dan TVL 11, mampu menghambat semua bakteri uji tersebut. Selain itu, pengujian menggunakan pelet dan supernatan menunjukkan bahwa 12 isolat mampu menghasilkan aktivitas antibakteri, dengan 5 isolat menunjukkan aktivitas pada supernatan. Isolat TVL 6 dan TVL 11 terbukti efektif menghambat semua bakteri uji baik pada pelet maupun supernatan, dengan penghambatan tertinggi terhadap Stapylococcus aureus. Hasil ini mengindikasikan bahwa bakteri asosiasi dalam saluran cerna bulu babi memiliki potensi sebagai kandidat penghasil antibakteri. 
PELATIHAN PEMBUATAN ECOENZYME "CAIRAN SERBA GUNA" SEBAGAI BAHAN ALTERNATIF BIO-HANDSANITIZER DAN BIOFERTILIZER PADA KELOMPOK TANI DESA SUKA SARI, KECAMATAN KABAWETAN, KABUPATEN KEPAHIANG Wibowo, Risky Hadi; Sipriyadi, Sipriyadi; Adfa, Morina; Hidayah, Thoriqul; Indah Medani, Della; Silvia, Elsi; Wahyuni, Reza
Martabe : Jurnal Pengabdian Kepada Masyarakat Vol 5, No 1 (2022): Martabe : Jurnal Pengabdian Kepada Masyarakat
Publisher : Universitas Muhammadiyah Tapanuli Selatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31604/jpm.v5i1.376-384

Abstract

Limbah organik berupa sayuran dan buah-buahan di Desa Sukasari, Kabupaten  Kepahiang, Kota Bengkulu yang melimpah dapat dijadikan sebagai produk Eco-enzyme sebagai bahan alternatif pembuatan Bio-handsanitizer dan Biofertilizer. Dari hasil kegiatan pengabdian kepada masyarakat mengenai aplikasi pemanfaatan ecoenzyme dari limbah organik sebagai bahan alternatif pembuatan Bio-handsanitizer dan Bio-fertilizer, para kelompok tani  telah mengetahui dan memahami cara pembuatan dan manfaat ecoenzyme. Hal ini menunjukkan kegiatan pengabdian sukses dan mencapai sasaran. Sosialisasi dan demonstrasi pelatihan yang dilakukan memberikan hasil persentase yang baik ketika pemberian umpan balik kepada peserta. Maka dari itu kegiatan pengabdian ini diharapkan untuk dapat memberi solusi terhadap kasus  pengolahan serta penanganan limbah organik melalui pembuatan produk ecoenzyme sebagai bentuk pengolahan limbah organik, seperti limbah sisa buah dan sayur dan diharapkan dapat memberi manfaat berupa wawasan untuk dimanfaatkan dalam realisasi produk ecoenzyme di Desa Suka Sari, Kecamatan Kabawetan, Kabupaten Kepahiang. Sehingga kedepannya kelompok tani dapat mengelola limbah organik secara tepat sehingga menghasilkan produk bermanfaat seperti biohandsanitizer dan biofertilizer dengan nilai jual yang tinggi dan diharapkan bisa mengurangi penggunaan pupuk kimia.
PENGAWETAN IKAN FILLET TUNA (Thunnus sp.) DENGAN KITOSAN MENGGUNAKAN METODE EDIBLE COATING Triani, Lensi Agus; Notriawan, Doni; Rahman, Refpo; Wibowo, Risky Hadi
Laboratory Journal : Jurnal Laboratorium Sains Terapan Vol. 2 No. 2 (2025): Desember
Publisher : Unib Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33369/jlst.2.2.33-45

Abstract

Ikan fillet tuna merupakan pengolahan inovasi ikan yang memiliki daya simpan yang singkat jika disimpan dalam suhu ruang. Penelitian ini bertujuan untuk memperpanjang daya simpan dan mempertahankan mutu fisik ikan fillet tuna dalam suhu ruangan dengan menggunakan lapisan kitosan. Penelitian ini menggunakan metode edible coating dengan merendam ikan fillet tuna pada larutan kitosan dengan berbagai variasi yakni tanpa perlakukan sebagai kontrol negatif, berbagai konsentrasi kitosan dari 1%, 1,5%, 2%, 2,5%, 3%, serta penggunaan formalin dengan konsentrasi 1% dan 10% sebagai kontrol positif selama 5 menit. Hasil menunjukkan bahwa ikan fillet tuna dengan konsentrasi 3% dapat memperpanjang daya simpan selama 4 hari sedangkan tanpa perlakuan sebagai kontrol negatif bertahan selama 2 hari, sampel dengan konsentrasi 1%, 1,5%, 2%, dan 2,5% memperpanjang daya simpan selama 3 hari. Maka dapat disimpulkan penggunaan larutan kitosan dapat digunakan sebagai pengawet ikan fillet tuna serta mempertahankan parameter fisik (tekstur, warna, aroma) dan pH ikan fillet tuna lebih baik setelah perlakukan dibandingkan sebelum perlakuan dengan larutan kitosan.
PENYULUHAN DAN PEMBUATAN PAPER SOAP (SABUN KERTAS) BAGI MASYARAKAT DI KELURAHAN PEMATANG GUBERNUR KOTA BENGKULU UNTUK MENINGKATKAN KESADARAN MENCUCI TANGAN SEBAGAI LANGKAH AWAL PENCEGAHAN WABAH COVID-19 Wibowo, Risky Hadi; Sipriyadi, Sipriyadi; Pertiwi, Reza; Hidayah, Thoriqul; Medani, Della Indah; Wulandari, Gustina Dwi; Rosianti, Nadya
Martabe : Jurnal Pengabdian Kepada Masyarakat Vol 4, No 1 (2021): Martabe : Jurnal Pengabdian Kepada Masyarakat
Publisher : Universitas Muhammadiyah Tapanuli Selatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31604/jpm.v4i1.317-324

Abstract

Hand is the main transmission pathogen entry into the body which is the part of the body that is most often in direct contact with other limbs and surrounding objects. The importance of maintaining hand hygiene by always washing hands with soap is the first step in preventing transmission of COVID-19. However, during a pandemic like this, it will be difficult to find soap available everywhere, and tends to be hard to carry all the time. Therefore, this service aims to invite the people of Pematang Gubernur, Bengkulu City, to use paper soap because it is easier to use its use and flexible to carry anywhere besides being an effort to prevent the COVID-19 outbreak, it can also add to new information and skills for residents in making attractive soaps. The feedback response given by the community service implementation team to the community service participants resulted in a positive response to this activity, who initially did not know about making and use of paper soap and became aware of this. 
Identifikasi dan Patogenisitas Agen Bakteri Penyebab Penyakit Busuk Batang pada Buah Naga (Hylocereus sp.) Fahmi, Riziq Ilham Nur; Cahlia, Uci; Wibowo, Risky Hadi; Sipriyadi, Sipriyadi
Spizaetus: Jurnal Biologi dan Pendidikan Biologi Vol 7 No 1 (2026): Spizaetus: Jurnal Biologi dan Pendidikan Biologi
Publisher : Program Studi Pendidikan Biologi, FKIP, Universitas Nusa Nipa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55241/spibio.v7i1.705

Abstract

The increasing intensification of dragon fruit (Hylocereus sp.) cultivation is followed by an increase in disease attacks that have the potential to reduce plant productivity. This study aims to isolate, identify, and test the pathogenicity of bacteria that cause dragon fruit stem rot in Pematang Donok Village, Kepahiang, Bengkulu. Bacterial isolation was carried out from disease-infected stem tissue, followed by isolation purification. Bacterial identification was carried out based on colony morphology and Gram staining, while pathogenicity and hemolysis tests were carried out on potatoes and blood agar media, respectively. The data were analyzed descriptively qualitatively through observation of colony morphological characteristics and Gram staining, as well as pathogenicity tests on potatoes and hemolysis tests on blood agar. The isolation results obtained two bacterial isolates, namely isolates BBNK1 and BBNK2 with different colony morphology characteristics. Both isolates are Gram-positive bacteria with bacillus and coccus cell shapes. Pathogenicity tests on potatoes showed that both isolates were able to cause soft rot symptoms such as tissue softening, discoloration, and mucus formation compared to the control. Hemolysis tests showed that only isolate BBNK1 showed hemolytic activity with the formation of a clear zone around the isolate. The findings of this study offer foundational data to support future research on molecular identification and the development of more environmentally friendly and sustainable disease management strategies.