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Journal : Bioscientist : Jurnal Ilmiah Biologi

Identification of Gastrointestinal Protozoa of Sumatera Elephant (Elephas maximmus sumatranus) in Lombok Wildlife Park Situmorang, Fernando Jose Immanuel Clinton; Kholik, Kholik; Atma, Candra Dwi; Riwu, Katty Hendriana Priscilia; Dharmawibawa, Iwan Doddy; Pradana, Munawer
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.11855

Abstract

The protozoan parasites have been reported to infect Sumatran elephants. Gastrointestinal protozoa could potentially be a factor in the decline in the Sumatran elephant population in Indonesia. This study aims to identify the presence of gastrointestinal protozoa in Sumatran Elephant (Elephas maximus sumatranus) in the Lombok Wildlife Park, North Lombok Regency, Indonesia. This research has used fresh fecal samples from 5 Sumatran elephants. The examination of feces samples using native, sedimentation, and floating methods. The research results have identified the presence of gastrointestinal protozoa in 2 of the 5 Sumatran elephant feces examined. The gastrointestinal protozoa found were Eimeria spp with dimensions of 16.30 x 20.93 μm and 25.21 x 38.49 μm.
Urine Analysis of Cat Utilizing the Thinka® Automatic Urine Analyzer as an Indicator for Uropoetic Disorders Riwu, Katty Hendriana Priscilia; Karina, Soca; Afnani, Daniah Ashri; Yansri, Alifianita Anake; Kholik, Kholik; Khairullah, Aswin Rafif
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 2 (2025): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i2.14738

Abstract

This study aims to determine the chemical content of the urine of local cats living freely around Mataram. The sample used was cat urine taken randomly. Twenty-five cats’ urine samples were put into a sterile urine pot. Urine chemistry examination and observation were carried out using a urine analyzer strip to identify the levels of urine chemistry, namely nitrite, protein, pH, blood, ketones, bilirubin, and glucose. The examination was done by dipping the strip into each urine sample; the strip was taken and tapped on the tissue, then inserted into the urine analyzer machine. After the results are obtained, the machine will automatically issue the results of the urine chemistry analysis, reading the results for no more than 60 seconds. This study emphasizes observing the quantity or amount of substances and sedimentation in cat urine samples. All of the 25 samples examined were positive (100%) for nitrite, protein, ketones, and glucose levels. The results of the blood level examination in urine were negative in all samples, the examination of urine pH levels in 25 samples varied with an average pH of 6.84, and the examination of bilirubin levels was positive in 24 (96%) urine samples. From the examination of the samples, there were indications of disorders of the uropoetic system and bacteriuria in cat urine.
Detection of HSP70 in Working Horses Using ELISA on Sumbawa Island Haddar, Mariyam Al; Pradana, Munawer; Kholik, Kholik; Supriadi, Supriadi; Satuman, Satuman
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 2 (2025): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i2.15197

Abstract

Heat shock protein 70 also known as HSP70 is a protein that regulates in response to stress factors such as heat, exercise, and others. The current research work used enzyme-linked immunosorbent assay (ELISA) to measure HSP70 levels in working horses on Sumbawa Island. Blood was taken through jugular venipuncture whereas saliva was collected non-invasively via cotton swab, both were analyzed with ELISA. The results indicated different expressions of HSP70; saliva shows a significantly higher level of HSP70 (52.07 ± 25.25 ng/mL) than serum (43.71 ± 34.83 ng/mL) from the same animal. The observed differences might indicate a better representation of acute stress by saliva due to physiological rapid turnover compared to serum, which reflects an integration of cumulative stress exposure. Our finding thus proves that double monitoring of both biological matrices offers complementary information in assessing stress in working horses while taking salivary HSP70 into account as especially promising for the in-field welfare monitoring due to its non-invasive collection and sensitivity to immediate stress conditions.