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THE POTENTIAL USE OF SIAM WEED (Chromolaena odorata) LEAF EXTRACT AS AN ALTERNATIVE ANTIBACTERIAL COMPOUND TO TREAT Vibrio parahaemolyticus INFECTION IN PACIFIC WHITE SHRIMP (Litopenaeus vannamei) Magfira, Magfira; Abidin, La Ode Baytul; Nur, Indriyani
Jurnal Riset Akuakultur Vol 19, No 3 (2024): September (2024)
Publisher : Politeknik Kelautan dan Perikanan Jembrana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jra.19.3.2024.177-190

Abstract

Siam weed plant or Siam weed (Chromolaena odorata) is an herb commonly used as a medicinal plant in Asian countries, including Indonesia, particularly in   Southeast Sulawesi. This study explores the effectiveness of different Siam weed leaf extract concentrations in treating Vibrio parahaemolyticus infection in Pacific white shrimp (Litopenaus vannamei). In this study, the infected shrimps were soaked in C. odorata leaf extract solution at 1, 2, and 3 ppt concentrations and no soaking of the extract (control). The parameters measured were recovery rate, survival rate, percentage of total haemocyte count (THC) and differential haemocyte count (DHC). The results showed that the V. parahaemolyticus-infected Pacific white shrimps soaked in 3 ppt C. odorata leaf extract had the highest recovery and survival rates compared to shrimp treated with C. odorata leaf extract at 1 and 2 ppt. Similarly, the shrimp group treated with 3 ppt of C. odorata leaf extract had better haemolymph profiles than those treated with the other concentrations of C. odorata leaf extract. This study concludes that C. odorata leaf extract enhances the immune response of L. vannamei by increasing the activity of semi-granular cells  in eliminating the pathogenic cells of V. parahaemolyticus.Tanaman krinyuh (Chromolaena odorata) merupakan tanaman herbal yang umum digunakan sebagai tanaman obat di negara-negara Asia, termasuk Indonesia, khususnya di Sulawesi Tenggara. Penelitian ini bertujuan untuk mengetahui efektivitas berbagai konsentrasi ekstrak daun tanaman krinyuh dalam mengobati infeksi Vibrio parahaemolyticus pada udang vaname (Litopenaus vannamei). Pada penelitian ini, udang yang terinfeksi direndam ke dalam larutan ekstrak daun C. odorata pada konsentrasi 1, 2, dan 3 ppt dan tanpa perendaman ekstrak (kontrol). Parameter yang diukur adalah tingkat kesembuhan, tingkat kelangsungan hidup, persentase jumlah hemosit total (JHT), dan jumlah hemosit diferensial (JHD). Hasil penelitian menunjukkan bahwa udang vaname yang terinfeksi V. parahaemolyticus yang direndam dalam ekstrak daun C. odorata 3 ppt memiliki tingkat kesembuhan dan kelangsungan hidup tertinggi disbanding udang yang diobati dengan ekstrak daun C. odorata pada konsentrasi 1 dan 2 ppt. Demikian pula, kelompok udang yang diberi 3 ppt ekstrak daun C. odorata memiliki profil hemolim yang lebih baik daripada yang diberi konsentrasi ekstrak daun C. odorata lainnya. Penelitian ini menyimpulkan bahwa ekstrak daun C. odorata meningkatkan respons imun L. vannamei dengan meningkatkan aktivitas sel semi-granular dalam menghilangkan sel patogen V. parahaemolyticus. 
Immunostimulation of Nile Tilapia Through the Provision of Synbiotic Feed (Eleutherine bulbosa and Probiotic) to Prevent Motile Aeromonas Septicemia (MAS) Disease Hasmin; Hamzah, Muhaimin; Abidin, La Ode Baytul
Journal of Fish Health Vol. 5 No. 3 (2025): Journal of Fish Health
Publisher : Aquaculture Department, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jfh.v5i3.7650

Abstract

Motile Aeromonas Septicemia (MAS) is one of the bacterial diseases that attacks and causes mass death of nile tilapia. Disease control in fish farming using synthetic antibiotics has caused problems in aquaculture, such as bacterial resistance to antibiotics, drug residues in fish, and water pollution. Therefore, alternative measures to control MAS that are safe and environmentally friendly, such as the application of immonostimulant that use organic ingredients, are needed. This research aims to determine potency of immunostimulation of nile tilapia trough feeding of synbiotic feed namely prebiotic Eleutherine bulbosa powder (Ebp) and probiotic containing Lactobacillus casei and Saccharomyces cerevisiae as for prevention MAS disease. The fish were reared for 21 days, in reared for the first 14 days, the fish were given synbiotic feed then for the next 7 days they were given feed without synbiotics. The fish were challenged with Aeromonas hydrophila on day 15th. Experimental design used was completely randomized design with four treatments: 0 g Ebp and 0 ml probiotics in 1 kg of feed (synbiotic free feed), 7.5 g, 10 g, and 12.5 g Ebp each with 15 ml probiotics in 1 kg of feed. The results showed that feeding the fish with synbiotic feed, especially the treatment Ebp12.5, give significantly different results on parameters prevalence, fish recovery, survival rate of the fish test. This research concluded that treatment of Ebp 12.5 was the best dosage of immunostimulant to prevent MAS disease in nile tilapia.
Application of Black Turmeric Rhizome (Curcuma aeruginosa Roxb) Ethanol Extract to Treat Aeromonas hydrophila Infection in Catfish (Clarias gariepinus) Mulfiani, Riri; Nur, Indriyani; Abidin, La Ode Baytul
Journal of Fish Health Vol. 5 No. 4 (2025): Journal of Fish Health
Publisher : Aquaculture Department, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jfh.v5i4.8609

Abstract

Aeromonas hydrophila is a pathogenic bacterium that frequently attacks, causes death, and harms catfish farmers. The continuous use of antibiotics for disease control has been shown to have negative impacts on pathogenic bacterial resistance, antibiotic residues in fish, aquatic environmental pollution, food safety, and causes allergies in human consumers. The use of phytopharmaceuticals is known to be more environmentally friendly and sustainable because they contain active compounds that are more easily degraded, thus leaving no synthetic antibiotic residues in fish tissues and water. This research aimed to determine the effect of black turmeric (Curcuma aeruginosa Roxb) rhizome ethanol extract on treating A. hydrophila infection in catfish. Fish were raised for 14 days. For the first seven days, they were kept under normal conditions. On day 7, the fish were infected with A. hydrophila. On day 8, the diseased fish were immersed in black turmeric rhizome ethanol extract. The experiment used group randomized design with treatment of control or without black turmeric extract (BT0), 35 mg/L, 40 mg/L and 45 mg/L black turmeric extract (BT35, BT40, and BT45). The result showed that treatments were not significantly different (P>0,05) on recovery rate, survival rate, and WBC, but significantly different in hematocrit percentage (P<0,05). The highest of fish recovery rate and survival rate (100%) were obtained in BT40. This research concluded that immersion dosage of 40 mg/L black turmeric rhizome ethanol extract could treating catfish infected with A. hydrophila.
Enhancement of Skin Pigmentation and Intestinal Health in Comet Goldfish (Carassius auratus) Using Dunaliella salina Teodoresco 1905 Supplemented Diets Iba, Wa; Martono, Martono; Syam, Rukman Awan; Ruslaini, Ruslaini; Abidin, La Ode Baytul; Kurnia, Agus; Nur, Indriyani; Rice, Michael A
Jurnal Ilmiah Perikanan dan Kelautan 2026: JIPK VOLUME 18 ISSUE 2 YEAR 2026 (JUNE 2026, ISSUE IN PROGRESS)
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v18i2.83250

Abstract

Graphical Abstract Highlight Research 1. Dunaliella salina meal (7–9%) optimally enhanced skin carotenoid deposition in C. auratus. 2. Higher D. salina inclusion improved intestinal morphology and reduced inflammatory lesions. 3. Intestine somatic index stabilized at 7–9%, indicating better digestive efficiency. 4. Survival rates increased with D. salina supplementation due to antioxidant and immunoprotected effects. 5. Microalgal carotenoids show strong potential as functional additives for ornamental fish feed.   Abstract Maintaining efficient and stable carotenoid-based pigmentation remains a challenge in aquaculture due to limited dietary carotenoid bioavailability and variable intestinal absorption. Comet goldfish (Carassius auratus) were used as a model species for aquaculture nutrition and digestive physiology to evaluate the effects of natural carotenoid supplementation from microalgae Dunaliela salina on skin pigmentation and intestinal health. This study aims to determine the effect of D. salina meal addition in formulated feed on the carotenoid content of fish skin, growth and survival as well as the intestinal condition of comet gold fish. Experimental diets were prepared by incorporating D. salina meal into a formulated feed at graded inclusion levels (0, 3, 5, 7, and 9%), where the microalgal meal was homogenized with other feed ingredients, pelleted, and oven-dried before use. The initial weight of experimental fish was 4 ± 1 g. The fishes were maintained in aquarium measuring of 40 × 35 × 35 (l × w × h) cm for 30 days and fed until satiation with 5 treatments of formulated feed added microalgae meal at 0, 3, 5, 7 and 9 %.  Commercial feed was used as control feed and all treatments were in triplicates. The highest final carotenoid content in skin of comet fish at 14.82 mg. g-1 was obtained with addition of 7 % microalgae meal.  The highest weight gain was obtained with addition of 5 % microalgae meal at 5.5 g.  Normal comet fish intestinal morphology was found in those feds on 9 % microalgae meal. Intestinal inflammation was observed in those feds with the addition of 3, 5 and 7 % microalgae meal and control feed.  The intestinal somatic index (ISI) value obtained was 1.08-3.06 %.  The survival was 100 % in fish fed with the addition of microalgae meal at high percentage from 5-9%. The findings provide practical guidance for developing natural, microalgae-based functional feeds that improve pigmentation efficiency, support intestinal health, and enhance survival, thereby contributing to more sustainable and economically valuable aquaculture production.  We recommend to use 7-9 % of D. salina meal for use in aqua feed to maintain optimal color, growth and survival of ornamental fish or cultured finfish in general.
Manajemen Kesehatan Ikan yang Dibudidaya di Kolam Batu Desa Kampoh Bunga Abidin, La Ode Baytul; Nur, Indriyani; Anwar, Asni; Hamsah, Hamsah; Bujang, Asis; Arsal, La Ode Muhammad; Aslin, La Ode; Sari, Sandra Mayang
Jurnal Pengabdian Magister Pendidikan IPA Vol 9 No 1 (2026): Januari - Maret 2026
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jpmpi.v9i1.14103

Abstract

Budidaya ikan dengan sistem kolam kurungan batu merupakan salah satu bentuk kearifan lokal masyarakat Desa Kampoh Bunga, Kabupaten Konawe Utara. Namun, sistem ini memiliki tantangan kesehatan ikan yang cukup tinggi akibat rendahnya penerapan biosekuriti, kurangnya pemahaman terhadap parameter kualitas air, serta minimnya penerapan imunostimulan berbahan alami (fitobiotik). Program Pengabdian kepada Masyarakat (PKM) ini bertujuan untuk meningkatkan kapasitas pembudidaya dalam manajemen kesehatan ikan melalui edukasi dan praktik langsung. Kegiatan meliputi penyuluhan biosekuriti dasar, manajemen kualitas air, dan demonstrasi fortifikasi pakan menggunakan fitobiotik melalui metode re-pelleting. Hasil kegiatan menunjukkan adanya peningkatan pemahaman dan keterampilan pembudidaya terhadap aspek biosekuriti, pengelolaan kualitas air, dan pemanfaatan imunostimulan alami. Peserta juga mampu mempraktikkan fortifikasi pakan secara mandiri menggunakan bahan-bahan lokal.