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Journal : Ahmar Metastasis Health Journal (AMHJ)

Soaked Water of Rice Straw Ash and Ziziphus mauritiana Leaf Extract as Disinfectant: A Microbiological Assessment Yulia, Yulia; Amaliyah, Nurul
Ahmar Metastasis Health Journal Vol. 5 No. 1 (2025): Ahmar Metastasis Health Journal
Publisher : Yayasan Ahmad Mansyur Nasirah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53770/amhj.v5i1.477

Abstract

Commercial dishwashing liquids contain chemicals that are extremely toxic to humans and the environment, hence alternative dishwashing using natural compounds is urgent. The purpose of this study was to evaluate the effect of a combination formula of rice straw ash and Ziziphus mauritiana leaf extract (JEBI) in reducing germs colonies per food equipment. This experimental study has a pre-post-test design with a control group. The variations of the JEBI combination used are 1:1, 1:2, 1:3, 2:1, and 3:1. Another treatment is using a commercial dishwasher brand "X" and only running water. A total of 210 cutleries (plates and bowls) from 30 restaurants in six districts were taken randomly—calculation of bacterial colonies using the Total Plate Count Agar method. The results were analyzed using one-way ANOVA to compare the effect between treatment groups on germ counts. Overall, a significant difference between the JEBI combination and the number of germs on food equipment was present in this study (p<0.001). The 1:3 JEBI ratio reduced the highest number of germs (average 24.6 colonies/cm2), slightly better than using "X" dishwashing liquid (average 25.6 colonies/cm2). Additionally, all treatments gave a significant reduction of germs compared to washed cutleries without any disinfectant (p<0.001). These findings strengthen the potential disinfectant function of rice straw ash and Ziziphus mauritiana leaf extract (JEBI) against germs on cutleries. Further studies with different ratio formulas and better study designs are required to obtain better and more precise results.
Co-Authors Adib, Moh. Afrianto, Rizki Ajinah, Ajinah Akhmad Taufiq, Mirwan Alexander Winggu Alfando, Yohanes Desidarus Amaria S.S wujon Ardhanty, Amelia Asriyani Tekol Aulia, Andi Rafika Rezky Aurelia Enomeko Beatrix A.K Lero Chusnul Khotimah Daflarosa R. Jaun Defrila K. Srirahayu DWI BHAKTI INDRI Elly Trisnawati Fathmawati Fathmawati Fathmawati Fikriyani, Mohammad Farhan Florida D.Y Jabur Fransiska N. Rado Fransiskus P. Ruku Fuad Hasim, Muhamad Gabriela A.L Keupung Hediyanti, Giska Hikmah, Kholisotul Indah Budiastutik Indah Purwaningsih Jaun, Daflarosa R. Karina Junita Kasrudin, Iqrayati Kausari, Ahmad Keupung, Gabriela A.L Khayan Khayan Kuswiyanto, Kuswiyanto M Baihaqi MaIik Saepudin Margaretha N. Pano Maria C. Koza Maria E.K Ola Maria F. Jaman Maria H. Gego Maria H.A Vera Maria M. Sasa Maria O.G Moni Marselina Eku Mathias F.B Weka Maulana, Rachmat Dwi Cahyo Misbach, Maulya Disti Misniarni Modesta Rengo Muflihah Muhammad Arafah Mutiara Nur Jasynda Nurhaliza, Vidiya Nurul Kharismadewi Pano, Margaretha N. Pranaka, Resky Nanda Purnomo, Aryanto R. Rindu Garvera Rachmawati, Emilia Rado, Fransiska N. Rafiyanti, Nuri Septi Ratih Lukmitarani Ratih Lukmitarani, Ratih Ratni A. Jue Regi Refian Garis Salbiah Salbiah, Salbiah Sasa, Maria M. Siti Helmyati Skolastika E. Kampas Slamet Wardoyo Sofiyana, Fitri Srirahayu, Defrila K. Suharno Sunarsieh Sunarsieh, Sunarsieh Suparmin Susilawati, Susilawati Syafa'at, Wahyu Syam, Rahmawati Syamsuar Manyullei Syamsul Bakhri Gaffar Taufik Anwar Tekol, Asriyani Teresia Y. Guna Thelma Issadora Ervenny Ursula H. Rampang Utami, Bunga Nuur Primayu Vidiya Nurhaliza Virgilius Duek Weka, Mathias F.B Yohanes P.L Kilok Yovantrinus Watu yulia Yulia Yulia