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CYTOTOXICITY ASSAY USING BRINE SHRIMP LETHALITY TEST ON COLLAGEN-CHITOSAN WOND DRESSING STERILIZED BY ULTRAVIOLET LIGHT Ary Andini; Endah Prayekti; Devyana Dyah Wulandari; Ersalina Nidianti
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 2 No 1 (2020): Laboratory Examinations Support in Medical Toxicology
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v2i1.1467

Abstract

Collagen gives a moist state on the wound area to accelerate the wound healing process. Chitosan is a polymer as known as non-toxic, antibacterial, antifungal, biodegradable, and biocompatible materials. Combination of collagen and chitosan expected to be the best biomaterials as a wound dressing for the healing process. The study aimed to determine cytotoxicity assay on collagen-chitosan wound dressing sterilized by ultraviolet (UV) Light using Brine Shrimp Lethality Test (BSLT) method. The test groups were divided into K0, K1, K2, and K3 groups. K0 contained pure chitosan as a control group, K1 contained collagen 25%-chitosan 75%, K2 contained collagen 50%-chitosan 50%, K3 contained collagen 75%-Chitosan 25%. Collagen extracted from skin and scalp of snakehead fish (Channa striata) then mixed with chitosan until formed collagen-chitosan wound dressing. This study used Brine Shrimp Level Test (BSLT) method with solution concentration 10, 50, 100, 250, 500, 750 and 1000 ppm. Based on the results showed that K0, K1, K2, and K3 group had LC50 >1000 that proven collagen-chitosan wound dressing was non-toxic materials. As conclusion of the study explained composite wound dressing based on collagen-chitosan in all groups that sterilized under UV-Light along 15 minutes was not toxic based on Brine Shrimp Lethality Test showed LC50 >1000.
PERANCANGAN PROGRAM PENENTUAN HISTOGRAM CITRA DENGAN GRAPHICAL USER INTERFACE (GUI) Ima Kurniastuti; Ary Andini
TEKNOLOGI DITERAPKAN DAN JURNAL SAINS KOMPUTER Vol 1 No 1 (2018): June
Publisher : Unusa Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/atcsj.v1i1.4

Abstract

Dalam penelitian ini dilakukan perancangan program penentuan histogram citra yang dilengkapi dengan Graphical User Interface (GUI) sehingga pengguna lebih mudah dalam mengoperasikan program. Perancangan dilakukan dalam beberapa tahap yaitu tahap perancangan desain GUI, perancangan GUI, perancangan program dan testing program. Perancangan desain GUI bertujuan untuk menghasilkan desain GUI yang akan mempermudah programmer dalam merancang GUI program. GUI program merupakan tampilan yang akan muncul saat pengguna mengoperasikan program. Tahap perancangan program berfungsi untuk membuat source code yang berisi perintah-perintah dalam program. Tahap terakhir yang dilakukan dalam penelitian ini adalah testing program dengan menggunakan sebuah citra. Dari hasil testing program dapat disimpulkan bahwa program penentuan histogram citra berjalan dengan baik.
Rancang Bangun Program Penentuan Fitur Tekstur Citra Kuku Jari Tangan sebagai Deteksi Dini Resiko Diabetes Mellitus Ima Kurniastuti; Ary Andini; Paramitha Nerisafitra
TEKNOLOGI DITERAPKAN DAN JURNAL SAINS KOMPUTER Vol 2 No 1 (2019): June
Publisher : Unusa Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/atcsj.v2i1.1138

Abstract

Pada penelitian ini dilakukan rancang bangun program penentuan ekstraksi fitur tekstur citra kuku jari tangan sebagai deteksi dini resiko diabetes mellitus dilakukan dalam beberapa tahapan yaitu persiapan data, perancangan desain program, perancangan program dan pengujian program. Tahap persiapan data dilakukan untuk mempersiapkan masukan program yaitu citra kuku jari tangan. Tahap perancangan desain program digunakan untuk merancang desain program yang mempermudah peneliti dalam merancang program. Tahap perancangan program dilakukan dengan merancang GUI program dan kode program untuk memberikan perintah pada GUI program. Tahap terakhir yaitu pengujian program dengan citra kuku jari tangan sebagai masukan program. Hasil pengujian program menunjukkan bahwa program berhasil menentukan fitur tekstur citra kuku jari tangan berupa mean, variance, skewness, kurtosis dan entropy serta dapat mengeksport hasil fitur tekstur citra ke dalam file excel.
STUDI PREVALENSI RISIKO DIABETES MELITUS PADA REMAJA USIA 15–20 TAHUN DI KABUPATEN SIDOARJO Ary Andini; Evy Sylvia Awwalia
Jurnal Ilmu Kesehatan dan Kesehatan Vol 2 No 1 (2018): FEBRUARY
Publisher : UNUSA Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/mhsj.v2i1.600

Abstract

Abstract: Worldwide number of adolescents diagnosed with diabetes mellitus has increased everyyear means that a prevention to reduce the prevalence of diabetes mellitus is required. The aim ofthis study was to determine the prevalence of diabetes mellitus risk in adolescents in Sidoarjo city,East Java and Diabetes Mellitus risk factors. Total population used in this study is composed by 150men and women aged 15–20 years who reside in various locations in Sidoarjo city. The obtaineddata were analyzed descriptively with cross-sectional design. The results showed the prevalence ofdiabetes risk in adolescents in Sidoarjo is about 42% with average glucose levels 104.35 ± 13.01mg/dl. The prevalence of non-diabetes mellitus in adolescents is 58% with the average glucose79.26 ± 7.01 mg/dl. The risk factor which lead to high risk of diabetes mellitus is fast-food consumption (71%), soft drink consumption (31%) and smoking (5%).
PENGARUH PEMBERIAN TOPIKAL EKSTRAK KOLAGEN KULIT IKAN LELE SANGKURIANG (CLARIAS GARIEPINUS VAR) TERHADAP TNF-Α DAN JUMLAH FIBROBLAST PADA LUKA BAKAR DERAJAT DUA TIKUS WISTAR Aisyah .; Zainatul Mufarikoh; Ary Andini
Jurnal Ilmu Kesehatan dan Kesehatan Vol 1 No 1 (2017): FEBRUARY
Publisher : UNUSA Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/mhsj.v1i1.611

Abstract

ABSTRACTBackground: Collagen has vital role on healing process of burn injuries, mainly in connective tissue.Collagen triggers fibroblast proliferation, support to form new granulation tissue and epithelium aroundwound. Wound treatment through collagen extract of Sangkuriang catfish skin can increase healing process.It stimulates humidity on level II burn injuries and encourage re-epithelization, proliferation and cellmigration also increases growth. Methods: This is an experimental studies using 4 groups of RattusNorvegicus (Wistar strain). All of the group get a burn injuries in their back skin. Control group 1 (K1) wastreated by lidocaine for three days and control group 2 (K2) for ten days. Whereas treatment group 1 (P1) getlidocaine and collagen extract from Sangkuriang cat fish for 3 days and treatment group 2 (P2) for 10 days.Result: Number of fibroblast/field of view on treatment group showed significantly increased comparedcontrol groups on the 3rd days (P=0,046) and the 10th (P=0,004). Percentage of TNF-α on level II-burninjuries in wistar rats showed significant difference. Percentage of TNF-α lower than treatment group andshowed significant lowering level compared control groups on the10th (P=0,022). Conclusion: Increasing offiroblast number and lowering level of TNF-α significantly showed collagen extract of Sangkuriang cat fishskin accelerate wound healing process.
MONITORING KADAR GLUKOSA DARAH SEBAGAI PENCEGAHAN DIABETES MELITUS BAGI MASYARAKAT DESA GELANG, TULANGAN, SIDOARJO Ary Andini; Andreas Putro Ragil Santoso
Community Development Journal Vol 2 No 1 (2018): Community Development Journal
Publisher : UNUSA Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (343.305 KB) | DOI: 10.33086/cdj.v2i1.382

Abstract

Kegiatan Pengabdian kepada Masyarakat (PkM) monitoring kadar glukosa darah sebagai pencegahan Diabetes Melitus dilaksanakan pada tanggal 14 Mei 2018. Kegiatan PkM ini merupakan hasil kerjasama dengan kader kesehatan setempat dalam rangka meningkatkan kualitas kesehatan masyarakat di desa Gelang, Kecamatan Tulangan, Kabupaten Sidoarjo. Peserta Pkm diikuti oleh masyarakat umum terutama para Lansia. Pelaksanaan kegiatan PkM dilakukan dalam dua tahapan yaitu pemeriksaan glukosa darah dengan menggunakan Point of Care Testing (POCT) dan konsultasi kesehatan. Berdasarkan hasil pemeriksaan menunjukkan bahwa 79% masyarakat memiliki kadar glukosa normal. Namun, masyarakat yang memiliki resiko diabetes (prediabetes) mencapai 19% dan peserta yang terindikasi terkena Diabetes Melitus adalah 2%.
ANALISIS RESIDU ANTIBIOTIK TETRASIKLIN PADA DAGING AYAM BROIlER DAN DAGING SAPI Siti Nur Aniza; Ary Andini; Indah Lestari
Jurnal SainHealth Vol 3, No 2 (2019): September 2019
Publisher : Faculty of Health Sciences Universitas Maarif Hasyim Latif

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51804/jsh.v3i2.600.22-32

Abstract

Tetracycline are antibiotics with broad-spectrum that used routinely in veterinary medicine for animal treatment and prevention from infectious diseases. But, consuming antibiotics for long time and exceeding standards can lead to accumulation of antibiotic residues in the body. Based on SNI 01-6366-2000, the maximum residual limit (BMR) of tetracycline antibiotic is 0.1 mg/kg in meat.The purpose of this study was to determine the concentration of tetracycline residues in broiler chicken meat and beef that obtained in East Surabaya namely Manyar market, Sinar Baru market, Ampengan Batu market, Tenggilis Mejoyo market, Krempyeng market, Soponyono market, and Gunung Anyar market. This study used Thin Layer Chromatography (TLC) as qualitative test and Spectrophotometric method as a quantitative test to determine the residual levels of tetracycline antibiotics. This study was conducted on March 15 onto May 2 2019. Based on the study, about 32 broiler chicken and 13 beef samples detected by the TLC method showed that had negative results, there was no containing tetracycline residues in samples. Awhile the results of the spectrophotometric method showed that as many as 31 samples of broiler chicken and 13 beef samples showed that had positive results containing residues of tetracycline antibiotics that exceed of the standard. Based on the results obtained that average concentration of tetracycline in samples of broiler chicken meat about 1,7 mg/kg and 0,8 mg/kg in beef samples. Descriptively,tetracycline residues in broiler chicken meat were higher than beef.
Analisa Kadar Kromium VI [Cr (VI)] Air di Kecamatan Tanggulangin, Sidoarjo Ary Andini
Jurnal SainHealth Vol 1, No 2 (2017): September 2017
Publisher : Faculty of Health Sciences Universitas Maarif Hasyim Latif

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51804/jsh.v1i2.105.55-58

Abstract

Tanggulangin is one of the sub-districts in Sidoarjo district affected by Lapindo mudflow caused by oil drilling system error located in the area of Siring, Porong. Therefore it had impacted the water quality in around Lapindo mudflow area. The aim of this research was to analysis the levels of water Cr (VI) Tanggulangin district. Water sample was analyzed by spectrophotometer with wavelength of 540 nm and done using two (2) steps ,which were(1) establishment of standard curve and (2) determination of Cr (VI) in water. Ten (10) water samples were taken from various villages in the subdistrict Tanggulangin. Based on the results on level of water chromium VI [Cr (VI)] analyze showed 4 of 10 samples had levels of water Cr (VI)higher than standard 0,05 mg/L (PP No.82 tahun 2001), those sample were 1, 3,4 and 10.
HUBUNGAN ANTARA KADAR BESI (Fe) AIR TAMBAK TERHADAP KADAR BESI (Fe) PADA DAGING IKAN NILA (Oreochromis niloticus) DAN IKAN BANDENG (Chanos chanos) DI KECAMATAN JABON SIDOARJO Siti Dzurrotul Ainiyah; Indah Lestri; Ary Andini
Jurnal SainHealth Vol 2, No 2 (2018): September 2018
Publisher : Faculty of Health Sciences Universitas Maarif Hasyim Latif

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51804/jsh.v2i2.258.21-28

Abstract

Jabon sub-district was one of located affected by Lapindo mudflow that had effect in water quality causing environmental pollution. One of the indicators of water pollution was heavy metal Fe exceeded of Fe standard about 0,3mg/L. When Fe was accumulated in the body could make irritation on skin and eyes, respiratory symptom, and could be caused cancer in long term. The purpose of research was to know correlation between Fe levels of pond water with Nile tilapia fish and Milkfish. This research used 10 samples of pond water, Nile tilapia fish and Milkfish were taken at same location in pond area of Jabon Sub-district. Analyzed of Fe was using Atomic Absorption Spectrophotometer (AAS). Based on the result indicated that Fe levels of pond water in Jabon sub-district was lower than Fe standard with mean about 0,015±0,445mg/L. Meanwhile Fe levels of Nile tilapia fish and Milkfish had mean about 4,783±0,444mg/kg and 6,6589±2,845mg/kg those were higher than Fe standard (> 0.5 mg/kg). It could be indicated that fish meat of Nile tilapia  fish and Milkfish were contaminated by Fe.Therefore, the results of pearson correlation test were shown no correlation between Fe levels of ponds water with Nile tilapia fish and Milkfish.
ANALISIS RESIDU ANTIBIOTIK TETRASIKLIN PADA DAGING AYAM BROIlER DAN DAGING SAPI ary andini
Jurnal SainHealth Vol 3, No 2 (2019): September 2019
Publisher : Faculty of Health Sciences Universitas Maarif Hasyim Latif

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51804/jsh.v3i2.448.33-36

Abstract

Tetracycline are antibiotics with broad-spectrum that used routinely in veterinary medicine for animal treatment and prevention from infectious diseases. But, consuming antibiotics for long time and exceeding standards can lead to accumulation of antibiotic residues in the body. Based on SNI 01-6366-2000, the maximum residual limit (BMR) of tetracycline antibiotic is 0.1 mg/kg in meat.The purpose of this study was to determine the concentration of tetracycline residues in broiler chicken meat and beef that obtained in East Surabaya namely Manyar market, Sinar Baru market, Ampengan Batu market, Tenggilis Mejoyo market, Krempyeng market, Soponyono market, and Gunung Anyar market. This study used Thin Layer Chromatography (TLC) as qualitative test and Spectrophotometric method as a quantitative test to determine the residual levels of tetracycline antibiotics. This study was conducted on March 15 onto May 2 2019. Based on the study, about 32 broiler chicken and 13 beef samples detected by the TLC method showed that had negative results, there was no containing tetracycline residues in samples. Awhile the results of the spectrophotometric method showed that as many as 31 samples of broiler chicken and 13 beef samples showed that had positive results containing residues of tetracycline antibiotics that exceed of the standard. Based on the results obtained that average concentration of tetracycline in samples of broiler chicken meat about 1,7 mg/kg and 0,8 mg/kg in beef samples. Descriptively,tetracycline residues in broiler chicken meat were higher than beef.
Co-Authors Ainiyah, Siti Dzurrotul Aisyah . Akhmad, Nur Faradila Alfri Danti Izky Oktavia Andreas Putro Ragil Santoso, Andreas Putro Ragil Angggraini, Rahayu Anggriyani, Rinda Anita Anita Anita Anita Anita Ardyarini Dyah Savitri Arsya Tazkiya Asror, Anita Rizki Rahmawati Atika Nur Laila Awwalia, Evy Sylvia Brina Thursina Dibiasi Budiarti, Rizqi Putri Nourma Chilyatuz Zahroh Devita Rahma Putri Deviyani, Dira Devyana Dyah Wulandari Edjianto, Soebagijo Poegoeh Endah Budi Permana Putri Endah Prayekti Endang Sulistiyani Ersalina Nidianti Evi Sylvia Awwalia Evy Sylvia Awwalia Fadillah Triasmoro Fadlillah Triasmoro Iskandar Fakurazi, Sharida Farah Nuriannisa Fauziah, Luqiana Fitria Agustina Gilang Nugraha Halimah Prisilia Hamiduumajid Ballihg Ballihgoo Hana Murni Kartika Sari Handayani Handayani Hermawati, Septina Isro’ Hidayat, Muhammad Taufiq Husein Firdaus Ibrahim Dwi Waluyo Putra Ima Kurniastuti Imratul Mukarramah Indah Lestari Indah Lestri Indah Nur Kamaliyah Irda Puspita Kamariyah, ST. Kardina, Rizki Nurmalya Khamida Khamida Khiliah Navis Khusnah, Hidayatul Kifli, Nurolaini Lili Susan Rahmawati Maghfuroh, Qonita Iklila Mardhotillah, Rachma Rizqina Marinda Dwi Puspitarini Mega Silvia Anggraini Sukri Moch Samsul Arifin Mohammad Nasir Mufarikoh, Zainatul Muhamad Damar Baskara Muhammad Afwan Romdloni Muhammad Raffli Muhammad Taufiq Hidayat Mukarramah, Imraatul Nathalya Dwi Kartika Sari Navis, Khiliah Nerisafitra, Paramitha Nila Qurrotul A’yun Nur Hamidah Paramitha Nerisafitra Prayekti, Endah Puspitasari, Adela Puspitasari, Renny Novi Rahayu Anggraini Rahmawati Fitri Awalia Rahmawati, Lili Susan Ratna Sofaria Munir Retno Diah Putri Ekayanti Rhomadhoni , Muslikha Nourma Riyan Sisiawan Putra Rizki Amalia Rizki Nurmalya Kardina Rizki Nurmalya Kardina Rohmah, Wardah Rukman, Nia Kurniaty Salimi, Syafrina Maulidyatus Salman Firmansyah Satya Nugraha Wirayudha Siska Wulandari, Siska Siti Dzurrotul Ainiyah Siti Husnul Khotimah Siti Nur Aniza Sofaria Munir, Ratna Soleha, Umdatus Sri Hartatik Suryandaru, Suryandaru Syafiuddin, Achmad Thomas Sumarsono Thomas Sumarsono Wandara Sekar Ayu Pramesti Wideistianti, Annisa Pricilya Widya Novita Sari Widya Novitasari Wijanarko, Robert Aditya Wulandari, Deyana Dyah Yauwan Tobing Lukiyono Zain, R.A Wafiqah Aulia Zainatul Mufarikoh