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ISOLASI SENYAWA 2-GERANIL-2’,3,4,4’-TETRAHIDROKSI DIHIDROKALKON DARI DAUN SUKUN (Artocarpus altilis (Park.) Fosberg) DENGAN FLASH COLUMN CHROMATOGRAPHY: Isolation of 2-Geranyl-2',3,4,4'-Tetrahydroxy Dihydrochalcone from Breadfruit Leaf (Artocarpus altilis (Park.) Fosberg) Using Flash Column Chromatography Yumni, Gharsina Ghaisani; Pertiwi, Krisna Kharisma; Widiyastuti, Yuli; Fakhrudin, Nanang
Jurnal Tumbuhan Obat Indonesia Vol. 16 No. 1 (2023): July 2023
Publisher : Universitas Tidar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31002/jtoi.v16i1.599

Breadfruit is one of the Indonesian plants traditionally used in medication. The main active compound in breadfruit leaves is a geranylated flavonoid namely 2-geranyl-2',3,4,4'-tetrahydroxy dihydrochalcone (GTD). Previous study showed that the separation and isolation of GTD from sukun leaves is time consuming and laborious as it requires a long procedure (extraction, liquid-liquid partition, Vacuum Liquid Chromatography (VCC), Sephadex Column Chromatography (SCC), and preparative Thin Layer Chromatography (TLC). This process is ineffective and inefficient. Thus, the more effective and shorter method of isolation is needed. This study aimed to isolate GTD from breadfruit leaves utilizing flash column chromatography (FCC). The breadfruit leaves were extracted using ethanol and the extract was partitioned with the solvent n-hexane: ethyl acetate: methanol: water (3:1:3:1). The lower phase containing GTD was subjected to VCC and the fraction containing GTD was purified with FCC (using n-hexane, ethyl acetate, and methanol in a gradient polarity as mobile phases; and silica gel as a solid phase) to isolate GTD. The isolated GTD was analyzed by thin-layer chromatography (TLC) and purity was determined using high-performance liquid chromatography. This method was able to produce 138 mg of GTD (purity of 88.49 %) from 15 g of breadfruit leaf extract (0.92% yield). This study demonstrated that GTD, a main bioactive compound of breadfruit leaves, could be effectively isolated by using FCC instead of SCC and preparative TLC.

POTENSI EKSTRAK ETHANOL DAUN BELUNTAS (Pluchea indica Less.) PADA PENYEMBUHAN LUKA BAKAR Pertiwi, Krisna K.; Rosa J. Hesturini; Dwi Wahyuni; Muhammad W Pambudi
Indonesian Health Literacy Journal Vol. 1 No. 2 (2024): Volume 1 Number 2 2024
Publisher : Suluh Adiluhung Publisher (SAPub)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70574/6ek01e91

Luka bakar merupakan kecelakaan yang sering terjadi di Indonesia. Masyarakat sering menggunakan bahan alam untuk mengatasinya, termasuk penggunaan daun beluntas. Penelitian ini bertujuan membuktikan potensi daun beluntas dalam penyembuhan luka bakar derajad II. Penelitian ini dilakukan secara eksperimental. Daun beluntas diekstraksi dengan ethanol 96% dengan perbandingan 1:10 selama 6 hari. Ekstrak kental yang didapatkan selanjutnya diuji skrining fitokimia dan KLT. Hewan coba yang digunakan adalah tikus putih jantan dibagi menjadi 5 kelompok, tiap kelompok terdiri dari 5 ekor. Setiap kelompok diberi perlakuan dengan kontrol positif, kontrol negatif, dan sampel dengan konsentrasi 5%, 15%, 25%. Ekstrak mengandung senyawa flavonoid, saponin dan tanin. Berdasarkan hasil pengukuran luka, masing-masing konsentrasi memberikan hasil yang berbeda secara signifikan (Sig=0,000) dan yang memberikan hasil yang paling baik adalah konsentrasi 25%.

Penetapan Kadar Kuersetin pada Obat Herbal yang Mengandung Ekstrak Tempuyung (Sonchus arvensis) Menggunakan KLT Densitometri Asih Imulda Hari Purwani; Krisna Kharisma Pertiwi; Dwi Wahyuni; Rachma Nurhayati
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.11260

Quercetin are the main marker compounds in extracts of Sonchus arvensis which show nephrolithiasis activity. For quality control of herbal medicines, Densitometric Thin Layer Chromatography (TLC) method has been developed in this study using quercetin as phytochemical markers. Objective: Determination for quercetin in herbal medicines. Determination of quercetin by determining the composition of the mobile phase, the wavelength of the analysis, the saturation time, the elution distance and the volume of the spot. The parameters observed in the optimization method and stability test are area, retardation factor (Rf), peak shape and resolution (Rs). The composition of selected mobile phase which results good separation and symmetrical peak shape is chloroform: methanol: dichloromethane: acetonitrile: formic acid (6: 2: 2: 0.05: 0.05 v/v/v/v/v) with a wavelength of 335 nm, time of saturation is 30 minutes, elution distance is 8 cm, volume of spots is 10 µL and Rf 0.48. In this study, it was shown that there were no factors that had a significant effect so that the method could be applied for the determination of quercetin levels in herbal medicines.The research data showed that the range of levels on quercetin 3.26%.

Karakterisasi dan Uji Aktivitas Antimikroba Bakteri Kandidat Probiotik dari Terasi Udang Rebon Herlinda Mawardika; Krisna Kharisma Pertiwi; Dwi Wahyuni; Qosania Wilda Aulia
Bioscientist : Jurnal Ilmiah Biologi Vol 11, No 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.8981

Probiotics are live microorganisms that are widely used to help maintain a healthy digestive system. Some compounds secreted by these bacteria have a bactericidal effect on pathogenic and putrefactive bacteria. Various types of probiotic bacteria can be obtained from fermented foods and drinks. This research aims to characterize bacteria from rebon shrimp paste in Macajah Village, Tanjung Bumi District, Bangkalan Regency, East Java Province which have potential as probiotics and test their antimicrobial activity against pathogenic bacteria. Bacteria were isolated using the Pour Plate technique using de Man Rogosa Sharpe Agar media. The bacterial isolates were then characterized by observing colony morphology, cells and biochemical properties. Next, antibacterial activity was tested using the well diffusion method. The results showed that the four bacteria obtained from the samples were gram positive, round and rod shaped, catalase negative, homofermentative, grew at a temperature of 37-45°C, survived at NaCl concentrations of 4 and 6.5%, low pH, and bile salts. According to the characterization results, three bacterial isolates belonged to the genus Enterococcus and one other isolate belonged to the genus Bacillus. Bacillus isolate is a potential probiotic candidate, because it has inhibitory activity against Escherichia coli and Staphylococcus aureus.

Uji Aktivitas Antioksidan Esktrak Kasar dan Terpurifikasi Daun Salam (Syzygium polyanthum) Krisna Pertiwi; Dwi Wahyuni; Rosa Juwita Hesturini
JUSTER : Jurnal Sains dan Terapan Vol. 4 No. 2 (2025): JUSTER: Jurnal Sains dan Terapan
Publisher : Jompa Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar

Bay leaves (Syzygium polyanthum) are one of Indonesia’s biological resources that have long been utilized, particularly as a cooking spice and in traditional therapy. Current use by the public is still largely based on empirical evidence, and scientific validation is still needed to support its application. The aim of this study was to determine the antioxidant capacity of both crude and purified bay leaf extracts. Extraction was carried out using the maceration method with 96% ethanol as the solvent. Purification of the extract was performed using a liquid-liquid extraction method with a solvent ratio of ethanol to n-hexane = 1:1. Phytochemical screening was conducted on both the crude and purified extracts. Antioxidant activity was evaluated using the DPPH method. Free radical scavenging was measured by UV-Vis spectrophotometry at the maximum wavelength. The results of phytochemical screening indicated that both crude and purified extracts contained alkaloids, flavonoids, tannins, and saponins. The maximum absorbance wavelength was found at λ = 514 nm. The antioxidant activity (IC₅₀) of the crude and purified bay leaf extracts was 15.120 ± 0.039 ppm and 13.293 ± 0.039 ppm, respectively. These results indicate that bay leaves possess strong antioxidant potential.

Paper Soap Herbal Kemangi dan Serai Sebagai Solusi Inovatif Pemberdayaan Petani Sayur Desa Janti, Papar Kediri Puji Lestari, Tri; Evi Kurniawati; Dwi Wahyuni; Esti Ambar Widyaningrum; Ida Kristianingsih; Nadia Pramasari; Krisna Kharisma Pertiwi
Room of Civil Society Development Vol. 4 No. 5 (2025): Room of Civil Society Development
Publisher : Lembaga Riset dan Inovasi Masyarakat Madani

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59110/rcsd.764

Kebersihan tangan merupakan aspek penting dalam pencegahan penyakit menular. Kegiatan pengabdian masyarakat ini bertujuan untuk meningkatkan pengetahuan dan keterampilan kelompok petani sayur di Desa Janti, Kabupaten Kediri, melalui workshop pembuatan sabun kertas berbahan ekstrak daun kemangi (Ocimum basilicum) dan serai (Cymbopogon citratus). Metode pelaksanaan menggunakan pendekatan partisipatif dengan praktik langsung, melibatkan 10 peserta. Instrumen evaluasi berupa kuesioner pra dan pasca workshop dianalisis secara deskriptif untuk menilai perubahan pemahaman dan keterampilan. Hasil kegiatan menunjukkan adanya peningkatan signifikan dalam aspek pengetahuan mengenai manfaat, bahan, dan proses pembuatan sabun kertas, di mana 90–100% peserta mampu memahami dan mempraktikkan tahapan produksi setelah workshop. Produk yang dihasilkan tidak hanya efektif sebagai agen antibakteri, tetapi juga memiliki nilai tambah dari sisi ekonomi dan lingkungan. Temuan ini menegaskan bahwa metode workshop berbasis praktik langsung efektif dalam membangun keterampilan sekaligus menumbuhkan potensi kewirausahaan masyarakat desa melalui pemanfaatan sumber daya lokal.

Paper Soap Herbal Kemangi dan Serai Sebagai Solusi Inovatif Pemberdayaan Petani Sayur Desa Janti, Papar Kediri Puji Lestari, Tri; Evi Kurniawati; Dwi Wahyuni; Esti Ambar Widyaningrum; Ida Kristianingsih; Nadia Pramasari; Krisna Kharisma Pertiwi
Room of Civil Society Development Vol. 4 No. 5 (2025): Room of Civil Society Development
Publisher : Lembaga Riset dan Inovasi Masyarakat Madani

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59110/rcsd.764

Kebersihan tangan merupakan aspek penting dalam pencegahan penyakit menular. Kegiatan pengabdian masyarakat ini bertujuan untuk meningkatkan pengetahuan dan keterampilan kelompok petani sayur di Desa Janti, Kabupaten Kediri, melalui workshop pembuatan sabun kertas berbahan ekstrak daun kemangi (Ocimum basilicum) dan serai (Cymbopogon citratus). Metode pelaksanaan menggunakan pendekatan partisipatif dengan praktik langsung, melibatkan 10 peserta. Instrumen evaluasi berupa kuesioner pra dan pasca workshop dianalisis secara deskriptif untuk menilai perubahan pemahaman dan keterampilan. Hasil kegiatan menunjukkan adanya peningkatan signifikan dalam aspek pengetahuan mengenai manfaat, bahan, dan proses pembuatan sabun kertas, di mana 90–100% peserta mampu memahami dan mempraktikkan tahapan produksi setelah workshop. Produk yang dihasilkan tidak hanya efektif sebagai agen antibakteri, tetapi juga memiliki nilai tambah dari sisi ekonomi dan lingkungan. Temuan ini menegaskan bahwa metode workshop berbasis praktik langsung efektif dalam membangun keterampilan sekaligus menumbuhkan potensi kewirausahaan masyarakat desa melalui pemanfaatan sumber daya lokal.

Edukasi Penggunaan Suplemen Selama Pandemi Covid-19 pada Kader Kesehatan di Wilayah Kerja Puskesmas Karangan Kabupaten Trenggalek: Education on the Use of Supplements During the Covid-19 Pandemic for Health Cadres in the Work Area of the Karangan Health Center Trenggalek Regency Widyaningrum, Esti Ambar; Kurniawati, Evi; Lestari, Tri Puji; Pertiwi, Krisna Kharisma
PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Vol. 8 No. 6 (2023): PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/pengabdianmu.v8i6.5547

Several ways can be used to boost the immune system, one of which is by using supplements that can be obtained through a doctor's recommendation or pharmacist consultation. Low immunity in humans will make it easy to be exposed to diseases or viruses, including quickly exposure to Covid-19. This is why many people take supplements: they think the amount of minerals and vitamins they get from their food is insufficient to keep their immune system healthy. One of the considerations for taking supplements is knowledge of their benefits. Even though supplements are not drugs, their use must also be rational. Individuals with adequate knowledge, especially about supplements, will tend to choose and use supplements according to conditions and benefits for the body. To find out the effectiveness of counseling in increasing knowledge of the use of supplements to increase body immunity during the Covid-19 pandemic for health cadres in the Karangan Health Center, Trenggalek Regency working area. This community service is conducted through lectures and question-and-answer method counseling. The number of participants in this activity was 33 health workers in the work area of the Karangan Health Center, Trenggalek Regency. Results: There was an increase in knowledge of the use of immunity-boosting supplements in health cadres in the work area of the Karangan Health Center, Trenggalek Regency, from the majority of sufficient knowledge (pre-test) to good (post-test). Education on using supplements can increase the knowledge of health cadres in the work area of the Karangan Health Center.

PERBEDAAN KADAR FLAVONOID KENTOS KELAPA (Cocos nucifera L.) YANG DIEKSTRAKSI DENGAN METODE BERBEDA Kurniawati, Evi; Lestari, Tri Puji; Pertiwi, Krisna Kharisma
PREPOTIF : JURNAL KESEHATAN MASYARAKAT Vol. 8 No. 1 (2024): APRIL 2024
Publisher : Universitas Pahlawan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/prepotif.v8i1.26952

Kelapa adalah tanaman yang memiliki banyak manfaat. Buahnya bernilai ekonomi tinggi, baik yang masih muda maupun yang sudah tua. Di dalam buah kelapa terdapat bagian kentos atau tombong kelapa, yang merupakan awal terbentuknya tunas kelapa. Kentos berbentuk bulat dan berada di dalam daging buah kelapa yang sudah tua. Kentos kelapa memiliki banyak khasiat dan mengandung berbagai zat gizi yang dibutuhkan manusia. Selain itu, kentos kelapa juga mengandung senyawa bioaktif, yaitu flavonoid. Untuk mendapatkan kandungan flavonoid dari kentos kelapa, perlu dilakukan proses ekstraksi. Kentos kelapa memberikan nutrisi yang sangat besar untuk tubuh dan masyarakat di Chennai- Tamil Nadu mengonsumsinya untuk mengurangi resiko tukak lambung. Kentos kelapa memiliki sifat kardioprotektif, termasuk sebagai antioksidan, dan juga memiliki kemampuan menurunkan infark miokard pada dosis 50, 100, dan 200 mg/100 g yang telah dibuktikanpada tikus jantan. Kentos kelapa sebagai bahan makanan memiliki banyak kandungan nutrisi yang dibutuhkan tubuh dalam proses metabolisme, di antaranya karbohidrat, protein, mineral, vitamin, dan lemak serta senyawa bioaktif seperti flavonoid dan terpenoid. Tujuan penelitian ini adalah untuk mengetahui apakah perbedaan metode ekstraksi berpengaruh terhadap kadar flavonoid dalam ekstrak etanol kentos kelapa (Cocos nucifera L.) menggunakan spektrofotometri UV-Vis. Hasil analisis kualitatif menunjukkan bahwa kentos kelapa positif mengandung flavonoid. Hasil analisis kuantitatif menunjukkan bahwa kadar flavonoid dalam ekstrak etanol daging kelapa bervariasi tergantung pada metode ekstraksi yang digunakan. Metode soxhletasi menghasilkan kadar flavonoid total tertinggi (94,159 mgQE/g), diikuti oleh metode perkolasi (44,418 mgQE/g) dan maserasi (19,630 mgQE/g).

Karakterisasi dan Uji Aktivitas Antimikroba Bakteri Kandidat Probiotik dari Terasi Udang Rebon Mawardika, Herlinda; Pertiwi, Krisna Kharisma; Wahyuni, Dwi; Aulia, Qosania Wilda
Bioscientist : Jurnal Ilmiah Biologi Vol. 11 No. 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.8981

Probiotics are live microorganisms that are widely used to help maintain a healthy digestive system. Some compounds secreted by these bacteria have a bactericidal effect on pathogenic and putrefactive bacteria. Various types of probiotic bacteria can be obtained from fermented foods and drinks. This research aims to characterize bacteria from rebon shrimp paste in Macajah Village, Tanjung Bumi District, Bangkalan Regency, East Java Province which have potential as probiotics and test their antimicrobial activity against pathogenic bacteria. Bacteria were isolated using the Pour Plate technique using de Man Rogosa Sharpe Agar media. The bacterial isolates were then characterized by observing colony morphology, cells and biochemical properties. Next, antibacterial activity was tested using the well diffusion method. The results showed that the four bacteria obtained from the samples were gram positive, round and rod shaped, catalase negative, homofermentative, grew at a temperature of 37-45Â°C, survived at NaCl concentrations of 4 and 6.5%, low pH, and bile salts. According to the characterization results, three bacterial isolates belonged to the genus Enterococcus and one other isolate belonged to the genus Bacillus. Bacillus isolate is a potential probiotic candidate, because it has inhibitory activity against Escherichia coli and Staphylococcus aureus.

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