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Kadar Fenolik Total dan Uji Aktivitas Antioksidan Ekstrak Kulit Batang Matoa (Pometia pinnata J.R.Forst. & G. Forst) dengan DPPH Islami, Deri; Setiawati, Yuyun; Putriani, Kony
JURNAL PROTEKSI KESEHATAN Vol 14 No 2 (2025): JPK: Jurnal Proteksi Kesehatan
Publisher : Poltekkes Kemenkes Riau

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Abstract

Matoa bark (Pometia pinnata J.R. Forst. & G. Forst) has long been used traditionally as a remedy for various ailments such as fever, skin disorders, infections, and inflammation. This study aims to determine the total phenolic content and antioxidant activity of matoa bark extract obtained through successive maceration using n-hexane, ethyl acetate, and 96% ethanol. Phytochemical screening revealed the presence of alkaloids, flavonoids, tannins, saponins, and triterpenoids. The results showed that the 96% ethanol extract had the highest total phenolic content at 169.804 mg GAE/g, followed by the ethyl acetate extract at 97.647 mg GAE/g, and the n-hexane extract at 52.549 mg GAE/g. Antioxidant activity tested using the DPPH method indicated that the ethanol extract exhibited the strongest activity with an IC₅₀ value of 92.593 μg/mL (strong category), followed by the ethyl acetate extract at 121.695 μg/mL (moderate category), and the n-hexane extract at 187.393 μg/mL (weak category). This study shows the role of polar solvents in increasing antioxidant activity and total phenolic content.
EVALUASI SEDIAAN MASKER GEL PEEL-OFF KOMBINASI EKSTRAK DAUN MANGGA BACANG (Mangifera foetida)DAN DAUN SALAM (Syzygium polyanthum) Putriani, Kony; Mardhiyani, Dini; Anggraini, Lovera
Jurnal Riset Kefarmasian Indonesia Vol. 4 No. 1 (2022): Jurnal Riset Kefarmasian Indonesia
Publisher : APDFI (Asosiasi Pendidikan Diploma Farmasi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33759/jrki.v4i1.218

Abstract

Mango Bacang leaves and bay leaves are plants that can be used as ingredients in traditional medicine as natural antibacterials which are believed to help get rid of acne. Several previous studies also reported that Bacang mango leaves and bay leaves have activity against Staphylococcus aureus, Escherichia coli and Salmonella typhi bacteria. This study aims to determine the evaluation results of peel-off gel mask preparations that meet the requirements. The test methods include organoleptic test, pH test, viscosity, homogeneity test and dispersion test. The test results showed that F1 and F2 were the best formulas with the characteristics of a green-black gel, a characteristic odor of mango Bacang and bay leaves, a viscosity of 1129 cPs and 1135 cPs, pH 4.4 and 4.5. The dispersion test was 5.1 cm and 5.7 cm and the homogeneity test on the peel off gel mask was homogeneous, there were no fibers, lumps or differences in color when applied to a transparent slide.
Uji Toksisitas Ekstrak Etanol 96% Daun Mahkota Dewa (Phaleria Macrocarpa) dengan Metode Brine Shrimpe Lethality Test (BSLT) Larysa Fernenda; Kony Putriani; Oksi Apriliani
Jurnal Ilmu Kesehatan Abdurrab Vol. 2 No. 1 (2024): Volume 2 Nomor 1 Maret 2024
Publisher : LPPM Universitas Abdurrab

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Abstract

Mahkota dewa (Phaleria macrocarpa) is a plant from the Thymelaeceae family. This plant contains several active compounds such as alkaloids, flavonoids, steroids, saponins, and tannins, so it has potential as a herbal medicine in Indonesia. This study aims to determine the toxicity of the leaves of the god crown using the Brine Shrimp Lethality Test (BSLT) method. Extraction of the god crown leaves using 96% ethanol solvent with the maceration method was then carried out a phytochemical screening which showed that the sample of the crown god leaf contained alkaloids, flavonoids, steroids, saponins and tannins. Toxicity testing of the ethanol extract of Mahkota Dewa leaves on Artemia salina Leach larvae was divided into 5 test groups, namely 4 treatment groups (concentrations of 250 ppm, 500 ppm, 750 ppm, and 1000 ppm) and 1 control or comparison group (seawater). Each concentration was made in 3 vials including 10 Artemia salina Leach larvae. Observations were made by looking at the death of Artemia salina Leach larvae after 24 hours of treatment. The research results can be seen through probit analysis by calculating the LC50 value. The LC50 value of the ethanol extract of Mahkota Dewa leaves was 7,7090 /mL. This shows that the ethanol extract of Mahkota Dewa leaves has a toxic effect on Artemia salina Leach larvae because the LC50 value is <1000 ppm.
Formulasi Sediaan Krim dari Ekstrak Kulit Batang Menteng (Baccaurea racemosa) Vonny Kurnia Utama; Kony Putriani; Sabrina Hayati
Jurnal Ilmu Kesehatan Abdurrab Vol. 2 No. 3 (2024): Volume 2 Nomor 3 September 2024
Publisher : LPPM Universitas Abdurrab

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Abstract

Stem bark of the menteng (Baccaurea racemosa) contains antioxidant phenolic and flavonoid compounds capable of stopping free radical chain reactions. This research aims to make a formula for cream preparation and to find out the antioxidant activity of cream extract from the stem bark of the menteng (Baccaurea racemosa). The study used experimental methods to make a 96% ethanol extract cream with concentrations of 0,5%, 1,5%, and 3%. The results of a semisolid shaped organoleptic test with a typical fragrance of brown, brown, and old brown brown bark extract, homogenity tests F0, F1, F2, and F3, are homogeneous. The pH tests F0 (5), F1 (4,5), F2 (4,7) and F3 (4,8). The spreadability tests F0 (6,2 cm), F1 (6,1 cm), F2 (5,9 cm) and F3 (5,6 cm). Viscosity tests F0 (887,73 cPs), F1 (748,06 cPs), F2 (839,43cPs) and F3 (817,03cPs). Testing antioxidant activity using UV-Vis spectroscopic photometers obtained a wavelength of 516,80 nm with an absorption value of 0,725 in formula 1 (extract concentration of 0,5%) obtained an IC50 value of 51,41, in formula 2 (extract concentration of 1,5%) obtained an IC50 value of 63,43, and in formula 3 (extract concentration of 3%) obtained an IC50 value of 48,91. The antioxidant content of formulas 1 and 2 has strong antioxidant activity whereas formula 3 has a significant amount of antioxidant activity.
TOXICITY OF ETHANOL EXTRACT FROM CHINESE LEUCAENA LEAF (Leucaena leucocephala) USING THE ARTEMIA SALINA BIOASSAY METHOD Kony Putriani; Wahyu Margi Sidoretno; Ayatu Fahmi
Jurnal Ilmu Kesehatan Abdurrab Vol. 3 No. 4 (2025): Volume 3 Nomor 4 Desember 2025
Publisher : LPPM Universitas Abdurrab

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Abstract

Chinese petai (Laucaena leucocephala) is a plant from the Fabaceae family. This plant contains several active compounds, including alkaloids, flavonoids, steroids, saponins, and tannins, making it a potential herbal medicine in Indonesia. This research aims to determine the toxicity of petai cina leaf extract using the Brine Shrimp Lethality Test (BSLT) method. The extraction process of Chinese petai leaves uses 96% ethanol solvent with the maceration method, then a phytochemical screening is carried out, which shows that the Chinese Petai leaf samples contain alkaloids, flavonoids, steroids, saponins, and tannins. Testing the toxicity of the ethanol extract of petai cina leaves on Artemia salina Leach larvae was divided into 5 test groups, namely 4 treatment groups (concentration 250 ppm, 500 ppm, 750 ppm, and 1000 ppm) and 1 control or comparison group (seawater). Each concentration was made in 3 vials containing 10 Artemia salina Leach larvae. Observations were made by looking at the death of Artemia salina Leach larvae after 24 hours of treatment. The research results can be seen through probit analysis by calculating the LC50 value. The LC50 value of the ethanol extract of petai cina leaves was 31.57342 µg/ml. This shows that the ethanol extract of petai cina leaves has a toxic effect on Artemia salina Leach larvae because the LC50 value is <1000 ppm.
Formulasi Obat Kumur Ekstrak Kulit Nanas sebagai Antibakteri Streptococcus mutans Kony Putriani; Senti Dwi Suryani; Nadya Putri Auliya Serawaidi; Nur Izzati
JURNAL PROTEKSI KESEHATAN Vol 15 No 1 (2026): JPK: Jurnal Proteksi Kesehatan
Publisher : Poltekkes Kemenkes Riau

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Abstract

Dental caries is an oral health problem that is caused by Streptococcus mutans. Pineapple peel is known to contain bioactive compounds such as flavonoids, tannins, saponins, phenolics, and bromelain, which have potential antibacterial properties. This study aimed to formulate an ethanol extract of pineapple peel into a mouthwash preparation and to evaluate its antibacterial activity against Streptococcus mutans. This research used an experimental laboratory method with the disc diffusion technique. The extract concentrations used were 5%, 10%, and 15%, which were then formulated into mouthwash preparations F1, F2, and F3. The evaluation of the formulations included organoleptic tests, pH, viscosity, and antibacterial activity. The results showed that the ethanol extract of pineapple peel at concentrations of 5%, 10%, and 15% produced inhibition zones of 12.1±1.05 mm, 12.0±1.00 mm, and 13.4±1.64 mm, respectively. Meanwhile, the mouthwash formulations F1, F2, and F3 produced inhibition zones of 11.1±0.1 mm, 11.5±1.2 mm, and 11.2±0.76 mm, respectively. ANOVA test results indicated a significant difference among treatments (p < 0.05). It can be concluded that the ethanol extract of pineapple peel formulated as a mouthwash exhibits strong antibacterial activity against Streptococcus mutans
Formulasi Gel Ekstrak Bonggol Nanas dan Aktivitas Antibakteri terhadap Staphylococcus aureus Muhammad Faruqi Perdana; Vonny Kurnia Utama; Kony Putriani; Ahridansyah
Jurnal Ilmu Kesehatan Abdurrab Vol. 4 No. 2 (2026): Volume 4 Nomor 2 Juni 2026
Publisher : LPPM Universitas Abdurrab

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Abstract

Bonggol nanas (Ananas comosus (L.) Merr.) mengandung bromelain dan metabolit lain yang berpotensi sebagai antibakteri. Penelitian ini bertujuan memformulasikan ekstrak bonggol nanas ke dalam sediaan topikal berbasis nanogel serta mengevaluasi karakteristik fisik dan aktivitas antibakterinya terhadap Staphylococcus aureus. Penelitian menggunakan desain eksperimental laboratorium. Bonggol nanas diekstraksi dengan metode maserasi menggunakan etanol 96%, kemudian diformulasikan menjadi empat sediaan, yaitu F0 tanpa ekstrak, FI ekstrak 1,5%, FII ekstrak 3%, dan FIII ekstrak 5%. Evaluasi meliputi organoleptis, homogenitas, pH, viskositas, pengukuran ukuran partikel, stabilitas selama 28 hari, dan aktivitas antibakteri menggunakan metode difusi sumuran. Seluruh formula berbentuk semi padat, homogen, dengan pH awal F0, FI, FII, dan FIII berturut-turut 5,50; 5,42; 5,33; dan 5,38. Viskositas awal berada pada rentang 16.356-31.917 cPs dan masih sesuai rentang viskositas gel. Pengukuran ukuran partikel belum dapat mengonfirmasi pembentukan nanogel karena sistem terlalu viskos, sehingga sediaan dikategorikan sebagai gel. Formula FII menghasilkan zona hambat tertinggi sebesar 9,00 mm dengan kategori sedang. Ekstrak bonggol nanas berpotensi sebagai bahan antibakteri alami dalam sediaan gel topikal, tetapi optimasi formula diperlukan untuk menghasilkan sistem nanogel yang ideal.