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Using Rhizhopora apiculata Extract for Mosquito Larvae Control Soni Sanjaya; Irwan Effendi; Nursyirwani Nursyirwani
Tropical Marine Enviromental Sciences Vol. 1 No. 1 (2022)
Publisher : Department of Marine Science, Faculty of Fisheries and Marine Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/tromes.1.1.25-31

Abstract

Temephos is the most commonly used synthetic larvicide to control vectors of several diseases. Currently in some areas there has been resistance of larvae to temephos, so natural larvicides are needed as an alternative. Rhizophora apiculata contains flavonoid compounds that are respiratory toxins to some larvae. This research was conducted in March - Juny 2022, to determine the inhibitory of mangrove extract against mosquito larvae in brackish water. Samples were collected from the Bakar Bakau Dumai, Riau Indonesia. The experiment was setted in a single factor experimental design. Mangrove leaves are finely ground and kneaded while mixed with water and deposited for 6 minutes and filtered. This extract solution was mixed with brackish water to obtain a test medium of 500 l with a concentration of 6 (a1), 9 (a2) and 12% (a3), positive control or 1 g of abate powder (a4) and negative control or brackish water (a0). A total of 20 mosquito larvae were put into the media and their mortality was observed at 0, 15, 30, 45 and 60 minutes after introduction. The mortality of larvae were then analyzed using probit analysis to obtain LC50 (Lethal Concentration 50) and LT50 (Lethal Time 50) values. R. apiculata extract is toxic to mosquito larvae, where larval death has been seen since 15 minutes and continues until 60 minutes after introduction. At the 60th minute the mortality rate is as follows; a1 (60 %), a2 (80 %), a3 (100 %), a4 (100 %), and a0 a1 ( %). Based on the results of the above probit values ​​in the LC50 and LT50 tests on R. apiculata leaves, the LC50 value is 9,732 while the LT50 value is estimated at 21,217
Supplementation of Fermented Moringa Leaves in Feed on Blood Glucose of Striped Catfish (Pangasianodon hypophthalmus) Infected with Aeromonas hydrophila Windarti Windarti; Irwan Effendi; Ronal Kurniawan; Mega Novia Putri; Dimas Gusriansyah; Okta Rizal Karsih
Jurnal Natur Indonesia Vol. 22 No. 2 (2024): October
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jnat.22.2.92-99

Abstract

Moringa leaves can be used as an herbal plant in fish growth to prevent and treat bacterial infections. This study aims to determine the effect of fermented moringa leaves through feed on blood glucose in striped catfish (Pangasianodon hypophthalmus). This research was conducted from September to November 2023 at the Marine Biotechnology Laboratory, Faculty of Fisheries and Marine Sciences, Riau University. The method used is an experimental method using a completely randomized design with 4 (four) treatments and 3 (three) replicates; the treatment dose of fermented moringa leaves is T0 (control), T1 (10 g/kg feed), T2 (15 g/kg feed), and P3 (20 g/kg feed). The catfish measured 5.00 ± 1.00 cm and weighed 4.00 ± 0.50 g, reared in an 80 L tank at a stocking density of 1 fish / 4 L water. The maintenance was carried out for 60 days, and blood glucose observations were made at the beginning (H-1), day 30 (H-30), day 60 (H-60), and post-challenge test (H-75). The results showed that adding fermented moringa leaves to the diet did not affect the blood glucose of the catfish (p>0.05). This indicates that moringa leaves can keep the fish in normal condition after being challenged with Aeromonas hydrophila bacteria.
Effect of Different Salinity on the Growth of Chlorella sp in Laboratory Scale Culture Irwan Effendi; Mega Novia Putri; Ulfi Laili Astika; Ronal Kurniawan
Jurnal Natur Indonesia Vol. 22 No. 1 (2024): April
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jnat.22.1.21-27

Abstract

This study aimed to determine the optimal salinity in increasing the growth rate of Chlorella sp in laboratory scale culture. This research was conducted in July 2023 at the Marine Biotechnology Laboratory, Faculty of Fisheries and Marine Sciences, Universitas Riau. The experimental method was applied using a complete randomized design (CRD) with four treatments and three replicates. The treatments were as follows: T0 (control), T1 (25 ppt salinity), T2 (30 ppt salinity), and T3 (35 ppt salinity). The initial density of Chlorella sp was 250x103 cells/mL, and cell abundance observations were carried out for 14 days. The parameters observed were cell abundance, specific growth rate, and water quality (temperature and pH). The results showed that different salinity influenced the growth of Chlorella sp. 35 ppt salinity gave the best results on cell abundance of 266.66x104 cells/mL, with peak population occurring on day 10. Water quality during the study was still in the normal range and can be tolerated for the growth of Chlorella sp, and the temperature ranged from 25-31oC and pH 6.4-8.0.
The Addition of Guava Leaves in Feed to the Blood Glucose of Carp reared in Brackish Water and Infected with Aeromonas hydrophila Irwan Effendi; Windarti Windarti; Heri Masjudi; Muhammad Rizal Razman; Ahmed Al-Harbi; Nisfi Maulidia Nasution; Tomi Syahputra; Ronal Kurniawan
Jurnal Natur Indonesia Vol. 21 No. 2 (2023): October
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jnat.21.2.156-161

Abstract

Guava leaves can be utilized as feed additives and medicine for aquaculture. Limiting factors in aquaculture, including stocking density, and salinity can cause stress in fish, so they are easily infected with disease. This study was conducted to determine the effect of guava on the blood glucose of carp reared in brackish water media. This research was conducted from August to September 2023 at the Marine Biotechnology Laboratory, Faculty of Fisheries and Marine, Universitas Riau. The method used in this study is an experimental method using a completely randomized design with 5 (five) treatments and 3 (three) replicates, the treatments are T0 (control), T1 (1.0g), T2 (1.5g), T3 (2 g), and T4 (2.5g). Carp measuring 5.00 ± 1.00 cm with a weight of 4.00 ± 0.50 g were obtained from farmers in the Koto Panjang hydroelectric reservoir, Sumatra, Indonesia. Fish rearing was carried out for 60 days. The results showed that the provision of guava leaves in the feed affected the blood glucose levels of carp. The dose of 1.5 g/100g feed (T2) was the optimal dose in increasing the blood glucose of carp reared in brackish water media and infected with A. hydrophila bacteria
Genetic Change of Pharella acutidens Influenced by Pb and Cd Metals in Rupat Strait Waters Tri Vani Bernadetha Ginting; Syafruddin Nasution; Irwan Effendi
Jurnal Natur Indonesia Vol. 19 No. 2 (2021): October
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jnat.19.2.43-50

Abstract

Rupat Strait related to the Strait of Malacca is a national and international shipping lane and makes the region affected by anthropogenic activities that result in a decrease in the quality of the waters such as the increasing amount of heavy metal content. The most easily contaminated  organism of heavy metals is bivalves due to and its low mobility, bivalves being filter feeders, and the ability to accumulate contaminants,. The purpose of  this study was to look at genetic changes in bivalves  (P.acutidens) contaminated by heavy metals. The study was conducted from February to March 2021. Bivalve sample was collected from the waters of Rupat Strait and sample analysis was conducted in the Marine Biology Laboratory and Genetics Laboratory of Riau University. Bivalve DNA is isolated with Geneaid Tissue Genomic DNA Mini Kit. DNA isolation results were then carried out PCR process and sequencing process was carried out in PT. Genetika Science Indonesia. The results of study show that sequencing results obtained by samples derived from Selinsing have the most changes in the composition of nitrogen bases compared to samples from the other two stations
Utilization of Sago Liquid Waste Organic Fertilizer as a Culture Medium for Chlorella sp. Irwan Effendi; Mega Novia Putri; Ulfi Laili Astika; Ronal Kurniawan
Jurnal Natur Indonesia Vol. 21 No. 1 (2023): April
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jnat.21.1.55-62

Abstract

Chlorella sp. is a microalga that can grow and develop in wastewater media, including sago liquid waste that is not utilized and becomes a pollution material. This study aims to determine the utilization of sago liquid waste organic fertilizer on the growth of Chlorella sp. This research was conducted in February 2023 at the Marine Biotechnology Laboratory, Faculty of Fisheries and Marine Sciences, Riau University. The method used was the experimental method, by applying a complete randomized design (CRD) with four treatments and three replicates. The treatments were as follows: T0 (control), T1 (150 ml sago liquid waste), T2 (200 ml), and T3 (250 ml). The initial density of Chlorella sp. was 250 x103 cells/ml, and cell abundance observations were made for 14 days. The parameters observed were cell abundance, specific growth rate, and water quality (temperature and pH). The results showed that the utilization of sago liquid waste affected the abundance of Chlorella sp. 150 ml concentration and gave the best results on cell abundance of 233.33 x103 cells/ml with the peak population occurring on day 9. Water quality during the study was still in the normal range and can be tolerated for the growth of Chlorella sp, namely temperature ranging from 26-31o C and pH 6.2-8.0.
HISTOPATHOLOGICAL OF STRIPED CATFISH (Pangasianodon hypophthalmus) REARED IN DIFFERENT SALINITIES Riswan, M; Syawal, Henni; Tang, Usman M; Effendi, Irwan; Kurniawan, Ronal; Wahyuni, Sri; Putri, Mega Novia
Asian Journal of Aquatic Sciences Vol. 8 No. 1 (2025): April
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/ajoas.8.1.1-6

Abstract

This study aimed to discover the effects of different salinities on histopathological Pangasianodon hypophthalmus. The method used is experimental by applying a completely randomized design (CRD) with four treatments: salinity 0 ppt, salinity 5 ppt, salinity 7 ppt, and salinity 9 ppt. The fish specimen is 8-10 cm in length and weighs 5 g. It was raised in a 54 L tank containing 40 L of water at a density of 2 fish 1 L-1 and kept for 45 days. Feed with commercial pellet 3 times a day in satiation. The results showed histopathological changes in gills and kidneys occurred at a salinity of 9 ppt. Chloride cell hypertrophy and secondary lamella bend in the gill organs, while the kidneys have acute tubular necrosis. Meanwhile, a salinity of 7 ppt resulted in abnormalities in the kidney organs with hemorrhage. It can be concluded that a salinity of 5 ppt indicates a safe salinity condition for the maintenance of striped catfish.
Effect of Detergent Concentration and Exposure Duration on the Growth of Heterotrophic Bacteria in Seawater Hafizh, Muhammad; Effendi, Irwan; Zulkifli, Zulkifli
Journal of Coastal and Ocean Sciences Vol. 6 No. 2 (2025): May
Publisher : Department of Marine Science, Faculty of Fisheries and Marine Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jocos.6.2.71-77

Abstract

This research was conducted from June to July 2024 at the estuary of the Selat Morong River, Rupat District, Bengkalis Regency, Riau Province. This study aimed to examine the effect of different detergent concentrations on the growth of heterotrophic bacteria, as well as to investigate the influence of varying detergent exposure durations on their growth. The experimental method employed a two-factor completely randomized design (CRD) consisting of different detergent concentrations (Factor A) with five levels: A1 (0 ppm) as the control, A2 (3000 ppm), A3 (6000 ppm), A4 (9000 ppm), and A5 (12,000 ppm), and varying exposure durations (Factor B): 1 day, 6 days, 11 days, 16 days, and 21 days. The results showed that the logarithmic values of heterotrophic bacterial growth over the 21 days indicated the highest growth occurred on day 1 at a concentration of 0 ppm, with a value of 7.09 CFU/mL. However, bacterial growth declined over time, reaching its lowest point on day 11 at a concentration of 3000 ppm, with a value of 6.95 CFU/mL. Statistical analysis revealed that different concentrations and exposure durations had no statistically significant effect on the growth of heterotrophic bacteria (P>0.05), meaning that variations in detergent concentration and exposure time did not cause meaningful differences in bacterial growth under the experimental conditions. This study highlights the potential resistance of heterotrophic bacteria to detergent exposure. It provides a basis for further research into the resilience of microbial communities in marine environments affected by pollution.
MANAGING CANNIBALISM AND IMPROVING LARVAL SURVIVAL IN BARRAMUNDI HATCHERY Lesmana, Indra; Effendi, Irwan; Suharman, Indra; Putra, Iskandar; Ruthmawati, Abigael; Andesca, Yofi Devara
Asian Journal of Aquatic Sciences Vol. 8 No. 2 (2025): August
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/ajoas.8.2.203-213

Abstract

Barramundi (Lates calcarifer) are economically valuable and usually cultivated in tropical marine hatcheries. Their fertilization and hatching rates are generally high, but poor survivorship of larvae due to cannibalism and cohort mismanagement remains a problem. In this study, we sought to address field-based techniques for controlling cannibalism and augmenting larval survival in large-scale hatcheries at Balai Besar Perikanan Budidaya Laut (BBPBL), Lampung, Indonesia. A 30-day rearing cycle was completed through descriptive observation methods focused on reproduction, and growth monitoring was performed concerning feeder grade improvement. The recorded data indicated a fertilization rate of 86% alongside an impressive hatching rate of 96%. However, the survival rate dropped to 52%, primarily due to cannibalism. Considerable size disparity among cohorts, lack of grading during asynchronous growth periods, and stunted development seem to drive cannibalism under these conditions. Increasing the frequency or decreasing the size gap between assessments has proven effective in enhancing survival levels alongside better feeding synchronization, water quality management, and reduced particulate matter concentration in water tanks. These outcomes will assist tropical aquaculture systems with long-term sustainable frameworks while providing actionable insight to hatchery managers without further testing
Structure of Intestinal and Kidney Tissue of Carp (Cyprinus carpio) Maintained in Salinity Media and Feed Enriched with Guava Leaf Flour (Psidium guava) Pulungan, Amar Doli; Windarti, Windarti; Eddiwan, Eddiwan; Effendi, Irwan; Kurniawan, Ronal
Jurnal Natur Indonesia Vol. 23 No. 2 (2025): October
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/jnat.23.2.136-143

Abstract

Carp (Cyprinus carpio) is one of the freshwater fishery commodities with high economic value. Keeping carp in saline media can affect the osmoregulation system, impacting the performance of vital organs such as the intestines and kidneys. Such disturbances can potentially reduce fish's health and survival rate. One strategy that can be used to increase the fish's immune system is through feed supplementation with natural immunostimulants, one of which is guava leaf flour (Psidium guajava), which is known to contain bioactive compounds such as flavonoids, tannins, and saponins. This study aims to evaluate the effectiveness of guava leaf flour-enriched feed on the histological structure of the intestines and kidneys of carp raised in saline media and exposed to Aeromonas hydrophila bacterial infection. The study was conducted from August to October 2024 at the Biotechnology Laboratory, Faculty of Fisheries and Marine, Universitas Riau. The method used was an experiment with a completely randomized design (CRD) consisting of five guava leaf flour dosage treatments with three replicates. The results showed that the histological structure of the intestine in all treatments was relatively normal, with neatly arranged villi and round to oval goblet cells. However, in the guava leaf powder supplementation treatment, several abnormalities were found in the kidney tissue, including inflammation, hemorrhage, and necrosis. This indicates that the salinity factor has a greater effect on kidney damage than the effect of immunostimulant administration. Nevertheless, the administration of guava leaf powder still positively improved the immune response and survival rate of fish. The optimal dose was obtained in treatment P2 (15 g/kg feed) with a survival rate of 83.33–93.33%. The water quality parameters during the study were still within the acceptable range for aquaculture, namely temperature 28–31°C, DO 3.7–7.4 mg/L, and pH 5.5–7. Thus, it can be concluded that feeding fish with guava leaf flour-enriched feed is effective in maintaining intestinal histology and increasing the survival rate of carp in saline media, although it cannot completely prevent kidney damage caused by environmental factors
Co-Authors abmi, cyndy Afrizal Tanjung Afrizani, Syafrinaldi Ahmed Al-Harbi Akbar, Sidiq Malik Andesca, Yofi Devara Andika Rismayanti Hadi ANDIKA SAPUTRA Anwar, Sagita Aqil Fikar Rivai Aras Mulyadi Arrafiqur Rahman Batubara, Ummi Mardhiah Bintal Amin Cahyani Fitrah Tanjung Christine M Situmeang Claudia, Kristiwany Mayneke Daulay, Anita Diah Permata Sari Dessy Yoswaty Dessy Yoswaty Dessy Yoswaty Desy Mutia Sari Dewan Pratama Novian Dimas Gusriansyah Dimas Gusriansyah Eddiwan Kamaruddin Efriyeldi, Efriyeldi Elizal Elizal Elizal, Elizal Eriani, Eti Eza Buana Fatwa Eza Buana Fatwa Fahmi, Adzra Ariesta Farma, Elmi Aulia Fathoni, Ahmad Yusron Feliatra Feliatra, Feli Fiki Harjuni firda laily mufid Ghifari, Muhammad Fadil Gusriansyah, Dimas Halima, Asyatul Hanies Ambarsari Hanryani, Putri Henni Syawal Henny Syawal Henny Syawal HERI SYAHFITRI Ida Ayu Eka Putri Yuliandari Ilham Ilahi Indra Lesmana Indra Suharman Irvina Nurachmi Irvina Nurrachmi Ita Widowati Joko Samiaji Judita Grace Merry Nainggolan Jusup Suprijanto Lubis, Nur Aisyah Lufita Nur Alfiah M. Yusuf Mardalisa, Mardalisa MARTONO MARTONO Masdini, Alvira Masjudi, Heri May Juna Tanjung Mega Novia Putri Mega Novia Putri Mega Novia Putri Mochammad Sukedi Moritania, Rizky Muhammad Aliv Kusuma Putra Muhammad Aliv Kusuma Putra Muhammad Arief Wibowo Muhammad Hafizh Muhammad Razali Muhammad Rizal Razman Muhson, Nanang Mukti, Pranata Rangga Mutia Dhiya Amani Mutia Dhiya Amani Mutiara Naimi Nasution Nasution, Nisfi Maulidia Nicholas Renaldo Ningsih, Irma Ardila Nisfi Maulidia Nasution Nuraini Nuraini Nursyafni Nanggola Nursyirwani Nursyirwani Nursyirwani Nursyirwani Nursyirwani, Nursyirwani Nyoto Okta Rizal Karsih Okta Rizal Karsih Parbo, Annisa Presti Povy Hutabarat Pulungan, Amar Doli Purba, Thadeus Mariode Triputra Purwantoro - Putra, Muhammad Hendra Putri, Mega Novia Rady, Rady Rika Permata Sari Rika Permata Sari Riswan, M Rita Wijaya Rita Wiyati Riza, Subkhan Roiman Pakpahan Ronal Kurniawan Ronal Kurniawan Ruthmawati, Abigael Serly Silviyanti Sibarani, Michael Parluhutan Jupiter Silitonga, Lamtiur Rotua Siti Ardiah Soni Sanjaya Soni Sanjaya Sony Sanjaya Sri Wahyuni Sri Wahyuni Suryadi Saputra Suryadi Saputra Syafruddin Nasution Syahputra, Tomi Syahril Nedi Syaputry Lita Yanti T. Budiardi Tampubolon, Kristanti Tanjung, Cahyani Fitrah Tengku Rany Oktaviany Tengku Rany Oktaviany Thamrin Thamrin Tomi Syahputra Tri Vani Bernadetha Ginting Tubagus Hasanuddin Ulfi Laili Astika Ulfi Laili Astika Usman Muhammad Tang Valentina Dian W. Cahyaningrum Windarti Windarti Windarti Windarti Windarti Windarti Yetti Elfina Yudia Anggun Kirana ZULKIFLI ZULKIFLI Zulkifli Zulkifli