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ISOLATION, IDENTIFICATION AND SENSITIVITY OF AMILOLITIC BACTERIA FROM MANGROVE ECOSYSTEM SEDIMENT IN PURNAMA MARINE STATION DUMAI ON THE PATHOGENIC BACTERIA Silitonga, Lamtiur Rotua; Nursyirwani, Nursyirwani; Effendi, Irwan
Asian Journal of Aquatic Sciences Vol. 2 No. 3 (2019): December
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

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Abstract

Litter from the weathering of dead mangrove stems and leaves contains a lot of starch which has potential to be degraded by amylolytic bacteria into simple compounds with the help of the amylase enzyme. Amylolytic bacteria are bacteria that hydrolyze starch into simpler compounds namely glucose with the help of the amylase enzyme. This study aims to 1) isolate, identify and test sensitivity of amylolytic bacterial isolates found at the Purnama Dumai Marine Station, 2) the ability of amylolytic bacterial isolates to inhibit the growth of pathogenic bacteria (Escherichia coli, Pseudomonas aeruginosa and Vibrio alginolyticus) and 3) to determine the of amylolytic bacterial species by 16S rRNA sequence analysis. The results showed 10 bacterial isolates (TR 2, TR 6, TR 7, TR 9, TR 11, TR 13, TR 15, TR 16, TR 18 and TR 20) were able to inhibit the growth of pathogenic bacteria (E.coli, P.aeruginosa and V.alginolyticus). The sensitivity test of isolate TR 20 against E.coli was categorized into weak with inhibition zone diameter of 4.65 mm. Sensitivity of isolate TR 6 against P.aeruginosa was categorized into medium with inhibition zone diameter of 5.22 mm. Then sensitivity of isolate TR 11 against V.algynolyticus was categorized into medium with inhibition zone diameter of 5.55 mm. DNA analysis using 16S rRNA method and BLAST analysis showed similarity of each isolate. Isolate TR 6 was similar to Bacillus paramycoides strain MCCC 1A04098, isolate TR 11 was in a group of Enterobacter cloacae strain ATCC 13047 and TR 20 was in a group of Vibrio harveyi strains of NBRC 15634
SCREENING THE ANTIBACTERIAL POTENTIAL OF Avicennia marina MANGROVE LEAF EXTRACT AS A NATURAL ANTIBIOTIC INGREDIENT Sibarani, Michael Parluhutan Jupiter; Effendi, Irwan; Yoswaty, Dessy
Asian Journal of Aquatic Sciences Vol. 7 No. 3 (2024): December
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (760.204 KB) | DOI: 10.31258/ajoas.7.3.462-469

Abstract

Bacterial resistance to antibiotics is a global health problem caused by two factors, namely natural resistance and acquired resistance. Several factors can cause problems. The problem in this research is whether Avicennia marina mangrove leaf extract found in the mangrove ecosystem in Bandar Bakau, Pangkalan Sesai Village, Dumai City, can be used as an antibiotic. This research was conducted in January-March 2024. This research aimed to determine the potential of mangrove leaf extract A. marina as an antibiotic against pathogenic bacteria Vibrio sp, Aeromonas sp, and Pseudomonas sp. The results of this research will provide benefits in the form of information regarding the potential of A. marina mangrove leaf extract as an antibiotic. The methods used in this research are survey methods and experimental methods. The samples were then analyzed using the paper disk diffusion method at the Marine Microbiology Laboratory, Faculty of Fisheries and Marine Science, Universitas Riau. The data obtained is then presented and explained descriptively. The research results showed that antibiotics added to A. marina leaf extract could inhibit the growth of A.hydrophila bacteria based on the inhibition zone formed of 11mm. A.marina contains alkaloids, terpenoids, and flavonoids and has antibacterial properties against A.hydrophila bacteria. Based on the data, it can be concluded that A.marina leaf extract can be used against pathogenic bacteria
ABUNDANCE AND DIVERSITY OF PHYTOPLANKTON IN THE WATERS OF TELUK LECAH VILLAGE, RUPAT, BENGKALIS Anwar, Sagita; Mulyadi, Aras; Effendi, Irwan
Asian Journal of Aquatic Sciences Vol. 7 No. 3 (2024): December
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/ajoas.7.3.329-335

Abstract

The aim of carrying out the research is to determine the abundance and diversity of phytoplankton. The method used in this research is a survey method, namely data collection, documentation, and direct observation in the field. Samples were analyzed at the Marine Biology Laboratory of Riau University. The results of the research show that there are six types of phytoplankton found in the waters of Teluk Lecah Village, Rupat District, Bengkalis Regency, namely Synedra sp, Dactylococcopsis sp, Nitzschia sp, Tribonema sp, Cylindrotheca sp, and Rhizosolenia sp. The first highest phytoplankton abundance was at station II, with an abundance of 0.1660, followed by the second highest phytoplankton abundance at station I, namely the residential area, with an abundance of 0.1494 Ind/L, while the lowest phytoplankton abundance was at station III, namely at the harbor with an abundance of 0. ,0996 Ind/L. The species diversity index at stations I and III, H' > 3, means the level of species diversity is high and the water conditions are not polluted. At station II, 1 ≤ H' ≤ 3 means the level of species diversity is moderate, and the water conditions are lightly polluted. It can be concluded that the H' value still meets the criteria. The species diversity index at all stations, E > 0.5, means that the uniformity of organisms is in balance, and there is no competition for certain places or food. It can be concluded that the E value also meets the criteria. Species dominance index at all stations, D < 0.5 means no dominant species exists
POTENTIAL OF VARIOUS INDONESIAN MEDICINAL PLANTS TO INHIBIT THE GROWTH OF Aeromonas hydrophila BACTERIA Kurniawan, Ronal; Windarti, Windarti; Effendi, Irwan; Putri, Mega Novia; Syahputra, Tomi; Gusriansyah, Dimas
Asian Journal of Aquatic Sciences Vol. 7 No. 2 (2024): August
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/ajoas.7.2.299-304

Abstract

Aeromonas hydrophila is a gram-negative bacterium that can infect fish and is zoonotic to humans. Efforts to prevent this bacterial infection can be made using herbal plants due to the content of secondary metabolite compounds. This study aims to explore plants that have the potential as antibacterials, especially A. hydrophila bacteria. This research was conducted in February 2024 at the Marine Biotechnology Laboratory, Faculty of Fisheries and Marine Sciences, Universitas Riau. The experimental method used included the stages of preparation of plant leaf samples and antibacterial tests using Kirby-Bauer discs. The plants used were the leaves of senduduk (Melastoma malabathricum), kantong semar (Nepenthes sp), lime (Citrus aurantifolia), belimbing bukit (Acetosella barreligeri), sirsak (Annona muricata), mucuna (Mucuna bracteata), Israel grass (Asystasia gangetica), and miana (Coleus scutellariodes). The results showed that the leaves of kantong semar, senduduk, and belimbing bukit could inhibit bacterial growth in a strong category. In contrast, the leaves of sirsak, mucuna, Israel grass, miana, and lime were classified as moderately inhibiting the growth of A. hydrophila bacteria. In conclusion, these herbal plants can potentially prevent A. hydrophila bacterial infection.
OPTIMIZATION OF Bacillus cereus GROWTH IN MEDIA WITH DIFFERENT CARBON SOURCES Putra, Muhammad Hendra; Feliatra, Feliatra; Effendi, Irwan
Asian Journal of Aquatic Sciences Vol. 4 No. 3 (2021): December
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

Bacillus cereus is a probiotic bacteria that requires media as a source of nutrients for its growth. This study aims to the media used were tofu liquid waste, sago liquid waste, and molasses. The method used is an experimental method, namely testing two groups of bacterial isolates; B. cereus SN7 and the consortium (combination of five isolates from B.cereus with different strains). Each group of isolates was grown with three replications on each waste medium. bacterial growth every six hours for twenty four hours using two methods; Total Plate Count (TPC) and bacterial cell biomass. There was no significant difference in growth between the two groups of bacterial isolation. The optimal growth of each isolate was found in molasses waste. The growth between two bacterial isolates in the liquid waste media was B. cereus SN7 bacterial isolate.
THE EFFECT OF DIFFERENT FEED ON THE GROWTH RATE OF Artemia salina Tampubolon, Kristanti; Effendi, Irwan; Tanjung, Afrizal
Asian Journal of Aquatic Sciences Vol. 3 No. 1 (2020): April
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

Artemia salina is a natural feed that is widely used in fish and shrimp hatcheries due to of its good nutritional content. This research was conducted in August 2019. This study aims to determine the effect of different types of feed on the growth rate of A. salina on a laboratory scale. The method used in this study was an experimental method that uses a completely randomized design (CRD) 1 factor with 3 level namely, cornstarch (A), wheat (B) and tapioca (C). Water parameters show that the conditions was in good condition with average temperatures ranging from 28.1 - 28.7 oC, the pH 8 and with a salinity of 60 ppt. Body length of A. salina ranged from 5.33 - 82.33 mm, where the body length in level A (cornstarch) was 82.33, level B (wheat) was 54.33, level C (tapioca) was 18, 67 and the control level is 5.33. The results of the ANOVA test for body length increase of A. salina showed p<0.05, that is 0.00. The survival rate of A. salina with different feed ranges from 0.5% - 88.50%, where the survival rate in level A (cornstarch) is 88.50%, level B (wheat) is 3.17%, level C (tapioca) is worth 51.50% and Control level is worth 0.5%. Survival rate in level A (cornstarch) is quite good. Based on ANOVA test, the survival rate between levels showed p<0.05, that is 0.00.
UTILIZATION OF MICROALGAE Scenedesmus vacuolatus AS A PHOSPHATE (PO43-) BIOREMEDIATOR IN DOMESTIC WASTEWATER Fathoni, Ahmad Yusron; Nursyirwani, Nursyirwani; Effendi, Irwan; Ambarsari, Hanies
Asian Journal of Aquatic Sciences Vol. 3 No. 1 (2020): April
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

This research was conducted in October 2019 to determine the effect of differences in the addition of KH2PO4 concentrations and the concentration of microalgae Scendesmus vacuolatus with experiment method. The results showed significant effect was found on differences in microalgae concentration and KH2PO4 concentration. KH2PO4 with the concentration of 8 ppm is the most influential on decreasing phosphate levels than KH2PO4 with 5 ppm. The highest samples in phosphate reduction were A2, B2 and C2 with different algal formulations and 8 ppm KH2PO4 concentration. Two-way ANOVA test showed sig. 0.000, Which was mean that there were significant effect to decrease levels of phosphate based on concentration. Post hoc DMRT which has a significantly different dominance value of KH2PO4 concentration results that A1, B1, C1 with A2, B2, C2 have significant differences in the decrease in phosphate levels due to differences in KH2PO4 levels.
Peningkatan Pengetahuan dan Kesadaran Pelajar terhadap Ekosistem Bakau di Bandar Bakau Kota Dumai, Provinsi Riau Elizal, Elizal; Effendi, Irwan; Nedi, Syahril; Tanjung, Afrizal
Journal of Rural and Urban Community Empowerment Vol. 5 No. 1 (2023): Oktober
Publisher : Fakultas Perikanan dan Kelautan Universitas Riau

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Abstract

Kegiatan pengabdian masyarakat ini bertujuan untuk meningkatkan pengetahuan dan appresiasi pelajar terhadap fungsi/peranan ekosistem bakau terhadap lingkungan, demikian juga tentang pentingnya upaya pengelolaan demi keberlanjutan pemanfaatannya. Pelaksanaan kegiatan menggunakan pendekatan dialogis yang dipandu oleh para instruktur. Kemudian mereka diajak instruktur berkeliling sambil menunjukkan salah satu jenis bibit bakau yang ad di kawasan tersebut. Kegiatan ini berjalan dengan baik, sesuai dengan yang diharapkan.
PELATIHAN PEMBUATAN BAKSO DAN NUGET IKAN BAGI IBU RUMAH TANGGA KAMPUNG MINAS BARAT KABUPATEN SIAK Effendi, Irwan; Wiyati, Rita
Journal of Rural and Urban Community Empowerment Vol. 1 No. 1 (2019): Oktober
Publisher : Fakultas Perikanan dan Kelautan Universitas Riau

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Abstract

Kurangnya minat makan ikan anak-anak Indonesia, mengakibatkan adanya penderita gizi protein dan penghambatan pertumbuhan. Ibu rumah tangga merupakan potensi tersendiri untuk pengembangan ekonomi dan nutrisi keluarga. Sebagian masyarakat mungkin tidak senang mengkonsumsi lele. Mungkin karena bau amisnya, bentuknya menakutkan bagi anak-anak, menjijikkan bagi sebagian orang tidak suka memakan ikan. Namun dengan dibuat menjadi bakso dan nugget orang akan lebih tertarik dan berminat untuk mengkonsumsinya. Kegiatan ini memberdayakan anggota kelompok dan menunjang perekonomian keluarga melalui pendampingan dan penyuluhan tentang teknologi pembuatan bakso dan nugget ikan. Para peserta diajari cara membuat bakso ikan dan nugget ikan. Strategi yang pertama dilakukan adalah dengan mengajar ibu-ibu nelayan untuk bergabung dalam program pengabdian ini, yaitu mereka akan dibentuk menjadi kelompok binaan yang berjumlah 20 orang dan menunjuk salah seorang diantara mereka sebagai ketua kelompok. Setelah kelompok terbentuk, maka akan dilakukan penyuluhan tentang manfaat ikan dalam bidang kesehatan maupun sebagai bahan dasar dalam pembuatan aneka jenis makanan olahan yang bernilai gizi dan ekonomi.
Teknik Budidaya Azolla microphylla pada Media Ember dan Kolam Terpal Effendi, Irwan; Ilahi, Ilham
Journal of Rural and Urban Community Empowerment Vol. 1 No. 1 (2019): Oktober
Publisher : Fakultas Perikanan dan Kelautan Universitas Riau

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Abstract

Azolla microphylla merupakan tanaman paku air yang mungkin masih belum terlalu di kenal masyarakat luas. Namun sebenarnya, tanaman paku air ini yang berada di dalam genus Azollaceae ini memiliki beragam manfaat, manfaat tersebut ada beberapa banyak terutama di dalam sektor pertanian dan juga perternakan, yaitu untuk pakan unggas, ikan dan bahkan untuk di jadikan pupuk. Kegiatan budidaya Azolla microphylla ini dilaksanakan di Kampus Marine Centre tepatnya di Laboratorium alam Bioteknologi Kelautan, Jurusan Ilmu Kelautan Fakultas Perikanan dan Kelautan Universitas Riau. Pelaksanaan pelatihan ini digunakan metode ceramah, diskusi dan langsung demonstrasikan kepada para mahasiswa dan dosen. Kegiatan ini diawali dengan penyuluhan tentang potensi Azolla microphylladengan menjelaskan tentang Teknik budidaya Azolla microphyllaserta manfaat dalam bidang perikanan.penanaman bibit Azolla microphylla terpal dan ember ketercapaian kegiatan terhadap tingkat tumbuh Azolla microphylla mencapai 80% dari total jumlah penanaman bibit yang dilakukan pada kolam terpal dan ember. Sedangkan 20% mengalami kegagalan yang diakibatkan faktor ketercapaian cahaya matahari yang masuk kurang terhadap perkembangan Azolla microphylla.
Co-Authors abmi, cyndy Afrizal Tanjung Afrizani, Syafrinaldi Ahmed Al-Harbi Akbar, Sidiq Malik Andesca, Yofi Devara Andika Rismayanti Hadi ANDIKA SAPUTRA Anwar, Sagita Aqil Fikar Rivai Aras Mulyadi Arrafiqur Rahman Batubara, Ummi Mardhiah Bintal Amin Cahyani Fitrah Tanjung Christine M Situmeang Claudia, Kristiwany Mayneke Daulay, Anita Diah Permata Sari Dessy Yoswaty Dessy Yoswaty Dessy Yoswaty Desy Mutia Sari Dewan Pratama Novian Dimas Gusriansyah Dimas Gusriansyah Eddiwan Kamaruddin Efriyeldi, Efriyeldi Elizal Elizal Elizal, Elizal Eriani, Eti Eza Buana Fatwa Eza Buana Fatwa Fahmi, Adzra Ariesta Farma, Elmi Aulia Fathoni, Ahmad Yusron Feliatra Feliatra, Feli Fiki Harjuni firda laily mufid Ghifari, Muhammad Fadil Gusriansyah, Dimas Halima, Asyatul Hanies Ambarsari Hanryani, Putri Henni Syawal Henny Syawal Henny Syawal HERI SYAHFITRI Ida Ayu Eka Putri Yuliandari Ilham Ilahi Indra Lesmana Indra Suharman Irvina Nurachmi Irvina Nurrachmi Ita Widowati Joko Samiaji Judita Grace Merry Nainggolan Jusup Suprijanto Lubis, Nur Aisyah Lufita Nur Alfiah M. Yusuf Mardalisa, Mardalisa MARTONO MARTONO Masdini, Alvira Masjudi, Heri May Juna Tanjung Mega Novia Putri Mega Novia Putri Mega Novia Putri Mochammad Sukedi Moritania, Rizky Muhammad Aliv Kusuma Putra Muhammad Aliv Kusuma Putra Muhammad Arief Wibowo Muhammad Hafizh Muhammad Razali Muhammad Rizal Razman Muhson, Nanang Mukti, Pranata Rangga Mutia Dhiya Amani Mutia Dhiya Amani Mutiara Naimi Nasution Nasution, Nisfi Maulidia Nicholas Renaldo Ningsih, Irma Ardila Nisfi Maulidia Nasution Nuraini Nuraini Nursyafni Nanggola Nursyirwani Nursyirwani Nursyirwani Nursyirwani Nursyirwani, Nursyirwani Nyoto Okta Rizal Karsih Okta Rizal Karsih Parbo, Annisa Presti Povy Hutabarat Pulungan, Amar Doli Purba, Thadeus Mariode Triputra Purwantoro - Putra, Muhammad Hendra Putri, Mega Novia Rady, Rady Rika Permata Sari Rika Permata Sari Riswan, M Rita Wijaya Rita Wiyati Riza, Subkhan Roiman Pakpahan Ronal Kurniawan Ronal Kurniawan Ruthmawati, Abigael Serly Silviyanti Sibarani, Michael Parluhutan Jupiter Silitonga, Lamtiur Rotua Siti Ardiah Soni Sanjaya Soni Sanjaya Sony Sanjaya Sri Wahyuni Sri Wahyuni Suryadi Saputra Suryadi Saputra Syafruddin Nasution Syahputra, Tomi Syahril Nedi Syaputry Lita Yanti T. Budiardi Tampubolon, Kristanti Tanjung, Cahyani Fitrah Tengku Rany Oktaviany Tengku Rany Oktaviany Thamrin Thamrin Tomi Syahputra Tri Vani Bernadetha Ginting Tubagus Hasanuddin Ulfi Laili Astika Ulfi Laili Astika Usman Muhammad Tang Valentina Dian W. Cahyaningrum Windarti Windarti Windarti Windarti Windarti Windarti Yetti Elfina Yudia Anggun Kirana ZULKIFLI ZULKIFLI Zulkifli Zulkifli