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Efek Ekstrak Kulit Durian (Durio zibethinus) Terhadap Kadar Glukosa Darah dan Histopatologi Hepar Tikus Wistar yang Diinduksi Aloksan Hura, Risna Pebriani; Budi, Andre; Suandy, Suandy
JUKEJ : Jurnal Kesehatan Jompa Vol 4 No 3 (2025): JUKEJ: Jurnal Kesehatan Jompa
Publisher : Yayasan Jompa Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.57218/jkj.Vol4.Iss3.2005

Abstract

Kulit durian (Durio zibethinus) kaya flavonoid yang berpotensi menurunkan glukosa darah dan melindungi hepar, namun bukti in vivo terstandar masih terbatas. Tujuan: Mengevaluasi efek ekstrak kulit durian terhadap kadar glukosa darah dan gambaran histopatologi hepar pada tikus Wistar yang diinduksi aloksan. Metode: Dua puluh lima tikus jantan diacak ke lima kelompok: kontrol negatif, kontrol positif (metformin 45 mg/kgBB), serta ekstrak 125, 250, dan 500 mg/kgBB (per oral). Glukosa darah diukur pra-induksi, pasca-induksi, hari ke-5, 10, dan 15; jaringan hepar dinilai semikuantitatif (nekrosis, kongesti, inflamasi) oleh penilai tersamar. Analisis menggunakan model berulang lintas waktu dan uji lanjutan yang sesuai. Hasil: Setelah induksi, terjadi hiperglikemia pada semua kelompok. Penurunan glukosa bermakna terlihat pada hari ke-10 dan 15 (p<0,05), dengan efek terbesar pada metformin, diikuti dosis ekstrak 250–500 mg/kgBB. Skor histopatologi hepar tidak berbeda bermakna antar kelompok. Kesimpulan: Ekstrak kulit durian menunjukkan aktivitas antihiperglikemik yang bergantung dosis, sementara bukti hepatoprotektif belum konklusif pada ukuran sampel ini. Diperlukan standarisasi ekstrak dan uji dengan daya (power) memadai untuk konfirmasi.
Strain Bakteri Asam Laktat SBM10 dari Matriks Berbasis Nabati Berupa Fermentasi Sirup Beras Merah: Evaluasi Keamanan In Vitro dan Karakterisasi Colin, Vito; Suandy, Suandy; Chiuman, Linda
Journal of Pharmaceutical and Sciences JPS Volume 8 Nomor 4 (2025)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v8i4.1099

Abstract

Plant-based fermentation offers a route to develop nondairy probiotic candidates, yet syrup-like matrices remain underexplored compared with solid or beverage systems. This study investigated fermented red rice syrup (prepared from red rice and barley malt powder) as a source of lactic acid bacteria (LAB) and advanced one isolate, SBM10. Cultivation on MRS supplemented with CaCO₃ yielded acidogenic colonies. A stable morphotype (SBM10) was purified and exhibited a LAB consistent profile, Gram-positive rods, catalase negative, TSIA K/A without H₂S, Simmons citrate negative, gelatinase negative, CO₂ positive in Durham and γ-hemolytic on 5% sheep blood agar. Under gastrointestinal stress models (4 h, 37 °C), tolerance relative to controls was 27.14% at pH 3.0 and 35.58% in 0.3% oxgall. In disk diffusion assays, the cell-free supernatant (CFS) showed no inhibition of Escherichia coli or Staphylococcus aureus. Thus, no antibacterial activity was detected by disk diffusion under the conditions tested, whereas amoxicillin produced zones of 13.95 mm and 12.43 mm, respectively. SBM10 presents a safety-supportive, LAB-typical profile with partial tolerance to gastric-like acidity and bile. Although no disk diffusion antagonism was detected for the CFS, the results motivate taxonomic confirmation and process/formulation optimization, such as improving acid/bile robustness and re-evaluating antimicrobial potential with complementary assays, to clarify the suitability of SBM10 for plant-based functional applications.
Efektivitas Ekstrak Bawang Putih Tunggal Terhadap Gambaran Histopatologi Ginjal Dan Kadar Ureum Kreatinin Rattus Novergicus Yang Mengalami Dislipidemia Rambe, Natasya Septiana; Suandy, Suandy; Chiuman, Linda
Syntax Literate Jurnal Ilmiah Indonesia
Publisher : Syntax Corporation

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36418/syntax-literate.v10i10.62239

Abstract

Heart disease and kidney failure are both increased by dyslipidemia. There are a lot of antioxidant and nephroprotective chemicals in just one clove of garlic (Allium sativum L.), including allicin, flavonoids, and organosulfur compounds. This study set out to examine the effects of a single garlic extract on the urea, creatinine, and uric acid levels in rats with dyslipidemia (Rattus norvegicus). There was an examination of the renal histopathology picture as well. This research used an experimental tack by using a control group design with pre- and post-tests. Each of the four groups consisted of 24 male Wistar rats' tails: the control group (aquades), the experimental group (simvastatin 2.1 mg/kgBB), the garlic extract alone group (200 mg/kgBB), and the highest dose (400 mg/kgBB). Each group followed a high-fat diet for a full month. Both the 200 mg/kgBB and 400 mg/kgBB doses of garlic extract significantly decreased the levels of ureum, creatinine, and uric acid (p<0.05) as compared to the negative control group. Kidney histopathology also revealed improvement, with less congestion, necrosis, and cell death. The positive control and the 400 mg/kgBB group showed similar effects after delivery. Rats with dyslipidemia showed improvements in renal histology and decreased levels of ureum, creatinine, and uric acid after administration of a single garlic extract.
Hubungan Konsumsi Kopi Dengan Kadar Asam Urat, Kolestrol, dan Body Mass Index (BMI) di Warkop Asli Medan Ananda, Rayhan Khozin Aulia; Lubis, Yolanda Eliza Putri; Suandy, Suandy
Syntax Literate Jurnal Ilmiah Indonesia
Publisher : Syntax Corporation

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36418/syntax-literate.v10i10.62247

Abstract

Coffee consumption has become part of people's lifestyle, especially among the productive age group. Coffee contains active compounds known to affect purine metabolism, lipid profile, and nutritional status. This study aims to analyze the relationship between coffee consumption and uric acid levels, cholesterol, and Body Mass Index (BMI) among consumers at Warkop Asli Medan. This study employed a descriptive observational design with a cross-sectional approach conducted from May to August 2025. A total of 96 respondents were selected using purposive sampling technique. Data were collected through questionnaires and direct examinations including uric acid levels, cholesterol, and BMI measurements. Data analysis used chi-square test with significance level of α = 0.05. The majority of respondents were male (65.6%) with moderate coffee consumption (59.4%). Examination results showed 81.3% of respondents had normal uric acid levels, 76.0% had normal cholesterol levels, and 60.4% had normal BMI. Bivariate analysis showed no significant relationship between coffee consumption and uric acid levels (p=0.426), BMI (p=0.160), and cholesterol (p=0.905). There is no significant relationship between coffee consumption and uric acid levels, cholesterol, and BMI among consumers at Warkop Asli Medan.
Uji Daya Hambat Kombinasi Ekstrak Daun Nanas (Ananas Comosus (L.) Merr.) dan Daun Sirih Hijau (Piper Betle L.) Terhadap Bakteri Propionibacterium Acnes dan Staphylococcus Aureus Zuhra, Layla; Lubis, Yolanda Eliza Putri; Suandy, Suandy
Syntax Literate Jurnal Ilmiah Indonesia
Publisher : Syntax Corporation

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36418/syntax-literate.v10i10.62296

Abstract

Jerawat vulgaris merupakan kondisi inflamasi kronis pada kelenjar pilosebasea yang ditandai dengan berbagai jenis lesi. Bakteri utama yang berperan dalam patogenesis jerawat adalah Propionibacterium acnes dan Staphylococcus aureus. Terapi antibiotik konvensional dapat menimbulkan efek samping dan resistensi, sehingga diperlukan alternatif antimikroba dari bahan alam. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri kombinasi ekstrak etanol daun nanas (Ananas comosus (L.) Merr.) dan daun sirih hijau (Piper betle L.) terhadap bakteri P. acnes dan S. aureus. Ekstrak dibuat dengan metode maserasi menggunakan etanol 96% pada konsentrasi 20%, 40%, dan 60%. Uji antibakteri dilakukan menggunakan metode difusi cakram (Kirby-Bauer) pada media Nutrient Agar, dengan klindamisin sebagai kontrol positif dan aquadest sebagai kontrol negatif. Hasil penelitian menunjukkan bahwa kombinasi ekstrak pada semua konsentrasi mampu membentuk zona hambat terhadap P. acnes (diameter rata-rata: 20,23 mm; 22,69 mm; 24,85 mm) dan S. aureus (diameter rata-rata: 20,51 mm; 20,23 mm; 24,74 mm), dengan zona hambat terbesar pada konsentrasi 60%. Analisis statistik menunjukkan perbedaan signifikan pada P. acnes (p = 0,016), namun tidak pada S. aureus (p = 0,284). Temuan ini mendukung potensi kombinasi ekstrak daun nanas dan sirih hijau sebagai alternatif agen antibakteri topikal untuk pengobatan jerawat, khususnya terhadap P. acnes. Penelitian lebih lanjut diperlukan untuk mengkaji mekanisme kerja dan keamanan penggunaannya.
DAMPAK PEMBERIAN SIRUP BERAS MERAH FERMENTASI BUBUK BARLEY TERHADAP BERAT BADAN TIKUS WISTAR DIABETES YANG DIINDUKSI ALOKSAN Suandy, Suandy; Aurellia, Fransisca; Nasution, M. Andriady Saidy
Jambura Journal of Health Sciences and Research Vol 6, No 4 (2024): OKTOBER: JAMBURA JOURNAL OF HEALTH SCIENCES AND RESEARCH
Publisher : Universitas Negeri Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35971/jjhsr.v6i4.27569

Abstract

Pengobatan farmakologis diabetes menghadapi tantangan berupa biaya tinggi dan resiko hipoglikemia, sehingga diperlukan alternatif pemanis pengganti gula. Kebaruan penelitian ini karena menggunakan beras merah yang difermentasi terhadap perubahan berat badan tikus diabetes. Tujuan penelitian untuk mengevaluasi dampak sirup beras merah yang difermentasi dengan bubuk malt barley terhadap berat badan tikus yang diinduksi aloksan. Penelitian ini menggunakan metode ekperimen murni dengan desain pre test dan post test yang menggunakan tikus wistar (Rattus norvegicus) pada empat kelompok yang dibagi acak yaitu K-, K+, P1, dan P2. Berat badan diukur pada hari ke-0, 7, 14, 21, dan 28. Hasil Tukey HSD menunjukkan bahwa terdapat perbedaan bermakna antara kelompok kontrol negatif dengan kelompok dosis 15 mg/kgBB dengan nilai p=0,023 (p 0,05). Kesimpulan penelitian ini bahwa pemberian sirup beras merah dengan dosis 15 mg/kg BB efektif dalam meningkatkan berat badan tikus diabetes. Hal ini mengindikasikan bahwa sirup beras merah bisa menjadi alternatif terapi untuk diabetes karena kandungan aktif dalam sirup beras merah berperan dalam perbaikan sel beta pankreas dan produksi insulin.
Effectiveness of Earthworm Extract on the Lipid Profile of Diabetic Wistar Rats Suandy, Suandy; Chiuman, Linda; Halim, Angel Jonathane
Jurnal Kedokteran Brawijaya Vol. 33 No. 2 (2024)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jkb.2024.033.02.3

Abstract

Various metabolic diseases, such as diabetes mellitus, are characterized by hyperglycemia, stemming from decreased insulin action, secretion, or both. The use of chemical medicines for diabetes mellitus, especially acarbose, involves regulating the digestion and absorption of complex carbohydrates. However, acarbose may disrupt liver function, prompting exploration into alternative sources for treatment, such as earthworm extract. The purpose of this study was to identify the effectiveness of ethanol extract and coelomic fluid from earthworms (E. eugeniae) on the lipid profile of male diabetic wistar rats (R. norvegicus) in vivo. The research hypothesis is that earthworm extracts could effectively lower the lipid profile of diabetic Wistar rats. This research is experimental research with pre-test and post-test control study group design, using an in vivo method. The results of the one-way ANOVA test of HDL were p=0.441; p=0.441; p=0.000; for LDL were p=0.691; p=0.101; p=0.049; for total cholesterol were p=0.107; p=0.347; p=0.486; and for triglycerides were p=0.028; p=0.926; p=0.553 on days 0, 7, and 14, respectively. Based on the research data, both ethanol extract and a combination of ethanol extract and coelomic fluid were able to reduce lipid profile levels on the 7th and 14th days. Earthworm extract has the potential to promote repair of β cells and is anti-inflammatory which can be used to reduce lipid profile levels.
Correlation of Wellness Program Against Degenerative Indicators Through Experimental Pre-Posttest Suandy, Suandy; Susianto, Susianto; Handoko, Erwin; Luwis, Kevin; Sanoesi, Vindelin
Jurnal Kedokteran Brawijaya Vol. 33 No. 2 (2024)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jkb.2024.033.02.4

Abstract

The incidence of degenerative diseases, such as hyperuricemia, hypercholesterolemia, obesity, hypertension, and diabetes mellitus, is increasing, primarily due to unhealthy lifestyles. Holistic treatment, involving pharmacology and lifestyle modification, is recommended. The Wellness program, covering diet, exercise, and relaxation techniques like meditation and yoga offers a holistic approach to reduce the incidence of degenerative diseases. Adopting a Wellness program could have positive outcomes, including cost-effectiveness, nutritional balance, and improvements in both physical (through yoga) and mental (through meditation) well-being, but the potential downsides include lack of control groups, inadequate consideration of age and gender, insufficient sample sizes, limited observation days, and an excessive number of measured parameters. This study aims to see the effectiveness and influence of the Wellness program on reducing uric acid levels, cholesterol, blood sugar levels, systolic and diastolic blodd pressure. The intervention is designed for women aged 30-60 who experience rage. However, individuals who are consuming medications or supplements, pregnant, breastfeeding, or not following a prescribed diet are excluded. This study used the quasi-experimental pre-posttest design to see the Wellness program’s effectiveness on uric acid, blood pressure, blood sugar levels, and total cholesterol. Sample size calculation was performed using G*Power version 3.1.9.7, and data analysis utilized the T-paired sampled test. The findings from a 3-day study involving 47 participants revealed significant differences post-implementation of the Wellness program, indicating a reduction in uric acid levels and an increase in blood sugar levels with a p-value<0.05.
Pengaruh Dukungan Keluarga Terhadap Kepatuhan Pasien Gagal Ginjal Dalam Terapi Hemodialisis Nasution, Ali Napiah; Suandy, Suandy; Manurung, Rachel Gita Berlian
Empiricism Journal Vol. 6 No. 4: December 2025
Publisher : Lembaga Penelitian dan Pemberdayaan Masyarakat (LITPAM)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36312/s1v4n576

Abstract

Penelitian ini bertujuan untuk mengetahui hubungan antara dukungan keluarga dan kepatuhan pasien gagal ginjal kronik dalam menjalani terapi hemodialisis di RSU Royal Prima Medan. Penelitian ini dapat berkontribusi pada praktik klinis atau kebijakan rumah sakit dalam meningkatkan kepatuhan terapi hemodialisis. Penelitian menggunakan desain analitik dengan pendekatan cross-sectional, melibatkan 57 responden yang ditentukan menggunakan rumus Slovin. Data dikumpulkan melalui kuesioner mengenai dukungan keluarga dan kepatuhan pasien, kemudian dianalisis menggunakan uji korelasi Spearman. Hasil penelitian menunjukkan bahwa mayoritas responden berusia di atas 40 tahun, berjenis kelamin laki-laki, berpendidikan SMA, serta telah menjalani hemodialisis selama satu tahun. Nilai rata-rata dukungan keluarga sebesar 42 menunjukkan kategori baik, sedangkan kepatuhan terapi memiliki nilai rata-rata 3,5 yang juga termasuk kategori baik. Hasil uji statistik menunjukkan nilai koefisien korelasi r = 0,309 dengan p < 0,001, menandakan adanya hubungan positif yang signifikan antara dukungan keluarga dan kepatuhan pasien hemodialisis, meskipun kekuatan hubungan tergolong lemah. Hal ini menunjukkan bahwa semakin baik dukungan keluarga, semakin tinggi pula tingkat kepatuhan pasien dalam menjalani terapi. Temuan ini menegaskan pentingnya peran keluarga dalam dukungan emosional dan motivasi agar pasien tetap konsisten menjalani pengobatan. Sehingga disarankan untuk meningkatkan dukungan keluarga di lingkungan rumah sakit atau klinis. The Influence of Family Support on Adherence to Hemodialysis Therapy in Kidney Failure Patients Abstract This study aims to determine the relationship between family support and adherence in patients with chronic kidney disease undergoing hemodialysis therapy at Royal Prima Medan General Hospital. This study can contribute to clinical practice or hospital policy in improving adherence to hemodialysis therapy. The study used an analytical design with a cross-sectional approach, involving 57 respondents determined using the Slovin formula. Data were collected through a questionnaire regarding family support and patient adherence, then analyzed using the Spearman correlation test. The results showed that the majority of respondents were over 40 years old, male, had a high school education, and had undergone hemodialysis for one year. The average value of family support was 42, indicating a good category, while adherence to therapy had an average value of 3.5, also in the good category. The results of the statistical test showed a correlation coefficient value of r = 0.309 with p < 0.001, indicating a significant positive relationship between family support and adherence in hemodialysis patients, although the strength of the relationship was relatively weak. This indicates that the better the family support, the higher the level of patient adherence in undergoing therapy. These findings emphasize the importance of the family's role in emotional support and motivation so that patients remain consistent in undergoing treatment. So it is recommended to increase family support in the hospital or clinical environment.
Hubungan Dukungan Keluarga, Kepatuhan Berobat, dan Tingkat Stres Terhadap Keberhasilan Adaptasi pada Pasien Diabetes Mellitus dengan Neuropati Diabetik di RS Royal Prima Medan Nasution, Ali Napiah; Suandy, Suandy; Zuhdi, Muhammad Fikri
Empiricism Journal Vol. 6 No. 4: December 2025
Publisher : Lembaga Penelitian dan Pemberdayaan Masyarakat (LITPAM)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36312/qanhp398

Abstract

Diabetes Mellitus (DM) adalah penyakit kronis dengan prevalensi tinggi secara global, di mana neuropati diabetik merupakan salah satu komplikasi yang sering dialami. Penelitian ini bertujuan untuk menganalisis hubungan antara dukungan keluarga, kepatuhan berobat, dan tingkat stres terhadap keberhasilan adaptasi pada pasien diabetes melitus dengan neuropati diabetik di RS Royal Prima Medan. Penelitian ini menggunakan desain kuantitatif dengan pendekatan cross-sectional. Data dikumpulkan melalui kuesioner dukungan keluarga berdasarkan model Nursalam yang terdiri dari 12 pertanyaan, kuesioner MMAS-8 untuk mengukur kepatuhan berobat, dan instrumen untuk mengukur tingkat stres. Data yang diperoleh dianalisis menggunakan uji Chi-Square dan Uji Fisher Exact Test apabila data tidak berdistribusi normal. Hasil penelitian menunjukkan bahwa sebagian besar pasien mendapatkan dukungan keluarga yang tinggi (73,3%) dan menunjukkan adaptasi yang baik (83,3%). Meskipun uji statistik menunjukkan tidak ada hubungan yang signifikan antara dukungan keluarga dan adaptasi pasien (p = 0,460), secara klinis, dukungan keluarga tetap memegang peranan penting dalam mendukung adaptasi pasien. Faktor-faktor lain seperti usia, tingkat pendidikan, dan durasi penyakit juga berperan dalam proses adaptasi. Temuan ini mengindikasikan pentingnya dukungan keluarga dalam pengelolaan diabetes melitus dan menyarankan perlunya program dukungan keluarga di rumah sakit. Penelitian lanjutan disarankan untuk mengeksplorasi faktor-faktor lain yang mempengaruhi adaptasi pasien, seperti tingkat stres, pendidikan, dan tingkat keparahan penyakit. The Relationship between Family Support, Treatment Compliance, and Stress Levels on Adaptation Success in Diabetes Mellitus Patients with Diabetic Neuropathy at Royal Prima Hospital Medan Abstract Diabetes Mellitus (DM) is a chronic disease with high global prevalence, and diabetic neuropathy is a common complication. This study aims to analyze the relationship between family support, medication adherence, and stress levels on the adaptation success of diabetes mellitus patients with diabetic neuropathy at Royal Prima Hospital Medan. This study uses a quantitative design with a cross-sectional approach. Data were collected using a family support questionnaire based on the Nursalam model consisting of 12 questions, the MMAS-8 questionnaire to measure medication adherence, and an instrument to measure stress levels. Data obtained were analyzed using Chi-Square test and Fisher Exact Test when data were not normally distributed. The results show that most patients received high family support (73.3%) and demonstrated good adaptation (83.3%). Although statistical tests showed no significant relationship between family support and patient adaptation (p = 0.460), clinically, family support plays an important role in supporting adaptation. Other factors such as age, education level, and disease duration also influence adaptation. These findings indicate the importance of family support in diabetes management and suggest the need for family support programs in hospitals. Further research is recommended to explore other factors affecting patient adaptation, such as stress levels, education, and disease severity.