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All Journal HAYATI Journal of Biosciences Jurnal Biotek Medisiana Indonesia Prosiding Seminar Biologi The Indonesian Biomedical Journal Universa Medicina Indonesian Journal of Obstetrics & Gynecology Science Acta Biochimica Indonesiana Eksakta : Berkala Ilmiah Bidang MIPA Majalah Patologi Indonesia Jurnal Biomedika dan Kesehatan Majalah Sainstekes World Nutrition Journal Jurnal Health Sains Muhammadiyah Journal of Geriatric Indonesian Archives of Biomedical Research (InABR) eJournal Kedokteran Indonesia IJRETINA - International Journal of Retina Jurnal Kedokteran Meditek Proceedings Book of International Conference and Exhibition on The Indonesian Medical Education and Research Institute

Sri Widia A.Jusman

Biochemistry Department, Faculty Of Medicine, Universitas Indonesia

Author-ID : 1802714

Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Energy Engineering Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Neuroscience Nursing Public Health Veterinary

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Peran Sitoglobin dalam Mencegah Stres Oksidatif Werdhasari, Asri; Prijanti, Ani Retno; Jusman, Sri Widia
Jurnal Biotek Medisiana Indonesia Vol 5, No 1 (2016)
Publisher : Puslitbang Biomedis dan Teknologi Dasar Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/jbmi.v5i1.5147.

Ekstrak Teh Hijau Mengurangi Luas Area Fibrosis Paru Tikus Desdiani; Iris Rengganis; Samsuridjal Djauzi; Agus Setiyono; Mohammad Sadikin; Sri Widia A. Jusman; Nurjati Chairani Siregar; Suradi; Putri C. Eyanoer
Majalah Patologi Indonesia Vol 29 No 1 (2020): MPI
Publisher : Perhimpunan Dokter Spesialis Patologi Indonesia (IAPI)

BackgroundPulmonary fibrosis is often found in infectious diseases and chemical exposure in the work environment. Antifibrotic effects can befibropreventive and fibrolysis. Bleomycin can cause inflammation and pulmonary fibrosis. Catechin is proven can repair liverdamages due to alcohol induction. The aim of this study is to examine the effect of antifibrotic green tea catechin on reducing thearea of rat lung fibrotic.MethodsThis research was carried out in vivo experimentally in the Laboratory Animal Management Unit at Faculty of Veterinary MedicineIPB in 4 groups of rat experiments, consisting of 3 rats in the negative control group, 3 rats in the positive control group, 3 rats in thegroup of rats given bleomycin on the 8th day and 28thwith green tea extract every day from day 0 to 49 days (fibropreventive), 3groups of rats given bleomycin days 0 and 21 with green tea extract every day from day 15 to 49 (fibrolysis). Histopathologicalexamination was performe for all groups of rats.ResultsThis study showed that administration of green tea extract in fibropreventive groups and fibrolysis groups could reduce the fibrosisarea in rat lungs based on Aschroft's modified scale.ConclusionBased on the results of this study it was found that the anti-fibrotic activity of green tea extract was proven to be able to suppressthe development of pulmonary fibrosis both by fibropreventive and fibrolysis

Expression of Hypoxia-Inducible Factor-1α and Myoglobin in Rat Heart as Adaptive Response to Intermittent Hypobaric Hypoxia Exposure Margaretha Herawati; . Wardaya; Wawan Mulyawan; Fanny Septiani Farhan; Frans Ferdinal; Sri Widia A. Jusman; Mohamad Sadikin
HAYATI Journal of Biosciences Vol. 24 No. 3 (2017): July 2017
Publisher : Bogor Agricultural University, Indonesia

The aim of this study was to investigate the influence of intermittent hypobaric hypoxia on the expression hypoxia adaptation proteins, namely hypoxia inducibla factor-1a (HIF-1a) and myoglobin (Mb). Twenty five male Sprague-Dawley rats were exposed to intermittent hypobaric hypoxia in a hypobaric chamber in Indonesian Air Force Institute of Aviation Medicine, for 49.5 minutes at various low pressure, 1 week interval for 4 times (day 1, 8, 15 and 22). HIF-1α and Mb protein were measured with ELISA. mRNA expression of Mb was measured with one step real time RT-PCR. HIF-1α protein levels increased after induction of hypobaric hypoxia and continues to decrease after induction of intermittent hypobaric hypoxia 3 times (ANOVA, p = 0.0437). mRNA expression and protein of Mb increased after induction of hypobaric hypoxia and continues to decrease after induction of intermittent hypobaric hypoxia 3 times (ANOVA, p = 0.0283; 0.0170), and both are strongly correlated (Pearson, r = 0.6307). The heart of rats adapted to intermittent hypoxia conditions by upregulation the expression of HIF-1a and myoglobin and then both return to normal level.

Efek Bubuk Susu Kedelai terhadap Kadar Kolesterol LDL dan HDL Serum Wanita Perimenopause dengan Hiperkolesterolemia Retno Kuntarti; Sri Sukmaniah; Sri Widia A. Jusman
Majalah Sainstekes Vol 4, No 2 (2017): Desember
Publisher : Lembaga Penelitian Universitas YARSI

Perimenopausal women is at risk of atherosclerosis as a result of the increase of LDL cholesterol level and the decrease of HDL cholesterol level. Soy milk powder consumption may protect toward these risk factors. This purpose of study is to investigate the effects of 2x30 g/d soy milk flour for eight weeks on serum cholesterol LDL and HDL levels in hypercholesterolemic perimenopausal women. The 19 subjects received 2x30 g/d soy milk powder for eight weeks. LDL and HDL serum cholesterol levels were determined at 0, 4, 9 weeks. Dietary intakes were assesed using 1x24 hours food recall. Statistical analysis used t Test. After eight weeks intervention, Soy milk powder decreases significant (p less than 0.05) LDL cholesterol levels at the 4th week (8.59 plus-minus 17.31%), and the8th week (7.81 plus-minus 11.32%). Soy milk powder can’t increase HDL cholesterol levels at the 4th and 8th weeks significantly. Soy milk decrease significant the ratio of LDL to HDL (7,03 plus-minus 16,82%) at the 4th week. Consuming soy milk powder 2 x 30 g/d during eight weeks can reduce the LDL cholesterol level and LDL/HDL ratio significantly.

Deskriptif Histologi Fase Wound Healing (Penyembuhan Luka) Pada Regenerasi Jaringan Ekor Cecak (Hemidactylus platyurus) Titta Novianti; Mohamad Sadikin; Vetnizah Juniantito; Sri Widia A Jusman; Evy Ayu Arida
Prosiding Seminar Biologi Vol 2 No 1 (2016): Prosiding Seminar Nasional From Basic Science to Comprehensive Education
Publisher : Jurusan Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/psb.v2i1.3305

Saat terjadi kerusakan pada jaringan setiap organisme, maka tubuh akan selalu berusaha untuk memulihkan kerusakan pada jaringan. Pada organisme multiseluler, regenerasi tidak hanya pemulihan struktur tetapi juga terjadi pemulihan fungsi jaringan. Proses regenerasi jaringan terjadi dalam empat tahap yang melibatkan berbagai jenis sel. Tahap pertama regenerasi adalah fase wound healing (penyembuhan luka), yang merupakan fase penentu berhasilnya proses regenerasi jaringan. Hasil penelitian secara deskriptif histologi dengan pewarnaan konvensional hematoksilin eosin, menggunakan model ekor cecak, terdapat berbagai jenis sel yang muncul dan berperan dalam proses regenerasi fase tersebut. Pada hari pertama dan hari ketiga setelah proses autotomi ekor cecak, tampak sel darah merah akibat perlukaan dan sel darah heterofil yang berperan mem-fagositosis sel dan jaringan yang mati, serta munculnya sel fibroblast. Sel fibroblast ini yang akan berperan dalam pembentukan jaringan baru. Pada hari kelima tampak pembentukkan pembuluh darah dan otot yang baru, serta pada hari kesepuluh terjadi pembentukkan lapisan sel epitel dan epidermis. Tahap regenerasi hari pertama sampai hari kesepuluh merupakan tahap penyembuhan luka. Setelah luka tertutup,mulai terjadi pertumbuhan berbagai jaringan di bawah epidermis. Kata kunci: regenerasi jaringan, histologi, ekor, cecak (Hemydactylus platyurus)

Negative Correlation between Cytoglobin Expression and Intracellular ROS Levels in Human Skin Keloid Fibroblasts Fajri Marindra Siregar; Novi Silvia Hardiany; Sri Widia Azraki Jusman
The Indonesian Biomedical Journal Vol 11, No 1 (2019)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v11i1.488

BACKGROUND: In our previous study, we found higher cytoglobin (Cygb) expression in keloid than normal tissue. Cytoglobin is a new globin family protein which function is still being studied to date. The purpose of this research is to elucidate the function of Cygb in human skin keloid fibroblasts (KFs), especially its role in intracellular reactive oxygen species (ROS) levels.METHODS: The study was conducted on human skin KFs obtained from primary culture. Inhibition of Cygb expression was achieved by using siRNA targeting Cygb. We compared the relative expression of Cygb between treatment and control group, and its effect on intracellular ROS levels. Gene expression was measured using quantitative real-time polymerase chain reaction (qRT-PCR) while the ROS level counted by dichlorodihydrofluorescein diacetate (DCFHDA) assay.RESULTS: There was an increase in intracellular ROS levels in the small interfering RNA (siRNA) (+) Cygb group compared to control group (1.673 vs. 1.260; 1.773 vs. 1.393; 1.710 vs. 1.360; respectively). There is a negative correlation between Cygb expression and ROS level (p<0.05; r=-0.651).CONCLUSION: There is a negative correlation between Cygb expression and intracellular ROS levels, we suggest Cygb acts as a ROS scavenger in human skin KFs.KEYWORDS: skin keloid fibroblasts, cytoglobin, siRNA, ROS

Secretomes of Primary Cancer-associated Fibroblasts Upregulate the Expression of Stemness Markers in HT-29 Human Colorectal Carcinoma Cells Septelia Inawati Wanandi; Dwi Retna Lestari; Noza Hilbertina; Nurjati Chairani Siregar; Sri Widia Jusman; Murdani Abdullah
The Indonesian Biomedical Journal Vol 12, No 4 (2020)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v12i4.1295

BACKGROUND: Cancer-associated fibroblast (CAF) is the most abundant tumor stroma. Our previous study has demonstrated that the secretomes of CAF isolated from colorectal carcinoma (CRC) patients could induce epithelial-mesenchymal transition in the HT-29 CRC cell line. However, the role of CAF secretomes in CRC stemness is needed to be further investigated. Therefore, the present study aimed to investigate the effect of CAF secretomes from CRC patients on the expression of stemness markers in HT-29 CRC cells in comparison with the secretomes from normal fibroblasts.METHODS: Fibroblasts were isolated from tumor (CAF) and their counterpart non-tumor (NF) areas of three CRC patients undergone surgical resection. Normal preputium fibroblasts (PF) were isolated during circumcision of three healthy boys aged 8 years. All fibroblasts were grown in free-serum culture medium for 24 hours to collect 50% (v/v) conditioned medium (CM). Then, CM was supplemented to HT-29 CRC cells for 72 hours. The effects of CAF- and NF-CM on the mRNA expression of CD44, CD133, OCT4, and ALDH1A1 were analysed using qRT-PCR. Cells proliferation was measured using the trypan blue exclusion assay.RESULTS: Supplementation of CAF-CM (50% v/v) significantly increased CD44, CD133, OCT4, and ALDH1A1 mRNA expressions compared to that of NF-CM and control without supplementation but had no effect on the proliferation of HT-29 cells.CONCLUSION: CAF secretomes from CRC patients upregulate the expression of CRC stemness.KEYWORDS: cancer-associated fibroblasts, ALDH1A1, OCT4, CD44, CD133, colorectal carcinoma

Carbon tetrachloride administration induces the expression of hypoxia inducible factor-1alpha in rat liver Lindi G Haritsyah; Mohamad Sadikin; Sri Widia Jusman
Acta Biochimica Indonesiana Vol. 1 No. 1 (2018): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.v1i1.3

Background: There is now increasing evidence that HIF-1 is also responsive to a variety of non-hypoxic stimuli. However, the mechanisms by which these non-hypoxic stimuli induce HIF-1α are not completely known, yet, although some evidence points to a role of ROS as messengers regulating HIF activity. Objective: To determine the expression of HIF-1α in liver rat tissue induced by carbon tetrachloride under normoxic conditions, with or without N-acetylcysteine protection. Methods: Twenty-five male Sprague-Dawley rats were divided into 5 groups: normal control rats, normal rats orally administered with coconut oil (1 mL/200 g body weight) for 1 day, rats orally administered with CCl4 (0.55 mg/g body weight) for 1 day, rats injected i.v. with NAC (0.15 mg/g body weight) for 8 days and then orally administered with CCl4 (0.55 mg/g body weight) for 1 day, rats orally administered with CCl4 (0.55 mg/g body weight) for 1 day and then injected i.v. with NAC (0.15 mg/g body weight) for 2 days. The expression of HIF-1α mRNA was measured by real-time RT-PCR using the Livak method. The expression of HIF-1α protein was measured by ELISA assay. Results: The highest HIF-1α mRNA and protein expression found in the group treated by CCl4 and then was gradually lowered in the pre-NAC group, post-NAC group, control group, and last, in the oil group. Conclusion: Our study shows the effect of CCl4-treated rats under normoxic conditions increased the mRNA and protein HIF-1α. NAC post-treatment provide a better protective effect compared with NAC pre-treatment

Expression of apelin is related to oxidative damage in heart tissue of rats during chronic systemic hypoxia H R Helmi; Frans Ferdinal; Ani Retno Prijanti; Sri Widia A Jusman; Frans D Suyatna
Acta Biochimica Indonesiana Vol. 1 No. 2 (2018): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.v1i2.18

Background: Chronic systemic hypoxia is severe environmental stress for the heart and might lead to the development of heart failure. Apelin is an endogenous peptide that has been shown to have various beneficial effects on cardiac function. Apelin appears to have a role to play in the ventricular dysfunction and maintaining the performance of the heart. Objectives: In the present study we want to investigate the adaptive response of heart tissue to chronic systemic hypoxia and the correlation with apelin expression and oxidative stress in rat. Methods: An experimental study was performed using 28 Sprague-Dawley male rats, 8 weeks of age. Rats were divided into 7 groups 4 each, namely control group; normoxia (O2 atmosphere) and the treatment group of hypoxia (8% O2) for 6 hours; 1;3;5;7 and 14 days respectively. Body weight and heart weight were measured at each treatment. Ventricular thickness was measured by caliper, Apelin mRNA was measured using real-time qRT-PCR with Livak formula and malondialdehyde (MDA) level was used to assess oxidative stress due to cardiac tissue hypoxia. Results: Macroscopic exams showed hypertrophy at day 7th. The relative expression of Apelin mRNA in hypoxic heart is decreased at the beginning and then increased, starting from day-7 to day-14. The MDA levels were significantly increased from day-7 and were strongly correlated with relative expression Apelin. Conclusion: It is concluded that the increase of Apelin expression is related to oxidative stress in heart tissue of rats during chronic systemic hypoxia.

Cytoglobin expression in rat kidney during exposure to systemic chronic hypoxia Ika Superti Daruningrum; Ani Retno Prijanti; Ninik Mudjihartini; Mohamad Sadikin; Sri Widia A Jusman
Acta Biochimica Indonesiana Vol. 3 No. 1 (2020): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.v3i1.55

Background: The kidneys in physiological conditions are always in a state of relative hypoxia. Cytoglobin (Cygb) is the newest globin protein found of the globin family. One of the functions of Cygb is in oxygen supply. Cygb expression is found to increase in hypoxic conditions, which are thought to be an adaptation response to hypoxia. Objective: This study aimed to analyze the expression of Cygb in rat kidneys which were exposed to chronic systemic hypoxia. Methods: Twenty five male Sprague-Dawley rats, weighing 150-200 g were used in this experiment. Rats were divided into 5 groups: The control group was exposed to normoxia; the hypoxia groups (10% oxygen / 90% nitrogen) for 1 day; 3 days; 7 days and 14 days. After treatment, rats were sacrificed and their kidneys were taken. Cygb mRNA expression was measured by qRT-PCR, while Cygb protein expression was measured by the ELISA method. Results: The expressions of Cygb mRNA and protein were found to be highest on day 3 of hypoxia and was correlated very strongly and significantly (r2 = 0.96; p <0.05). Conclusion: The highest expression of Cygb on day 3 of chronic systemic hypoxia exposure is suggested as an attempt to restore oxygen supply to the kidneys.

Co-Authors . Rusdi Abdul Halim Agus Setiyono Ahmad A Jusuf Ahmad Aulia Andi Arus Victor Andriani Andriani Angelina Stevany Regina Masengi Ani R Prijanti Ani Retno Prijanti Cindy Fawwaz Roviqoh Damar Prasmusinto Deasyka Yastani Desdiani Dewi Sukmawati DWI HASTUTI Dwi Hastuti Dwi Retna Lestari Ernie Purwaningsih Evy Ayu Arida Fadilah Fadilah Fanny Septiani Farhan Febriana Catur Iswanti Ferdiana Yunita Frans D Suyatna Frans Ferdinal Frans Ferdinal H, Novi Silvia Helmi Rizal Hendrik Kurniawan Heri Wibowo Ika Superti Daruningrum Indriati P Harahap Irawan Satriotomo Iris Rengganis Joedo Prihartono Joedo Prihartono Johannes Cornelius Mose K Kartini Kenny Cantika Abadi Labibah Qotrunnada Lailan Safina Nasution Lindi G Haritsyah Lis Rosmanah M Ekawati Maftuhatun Fista Amalia Mohamad Sadikin Mohamad Saekhu Mohammad Sadikin Mohammad Sadikin Muhammad Yamin Murdani Abdullah Ninik Mudjihartini Noroyono Wibowo Novi Resistantie Novi Silvia Hardiany Novianti, Titta Noza Hilbertina Nur Ita Margyaningsih Nurhadi Ibrahim Nurhadi Ibrahim Nurjati Chairani Siregar Nurjati Chairani Siregar Putri C. Eyanoer Rahayuningsih Dharma Raisa Nauli Reni Paramita Reni Paramita Ressa Yuneta Retno Kuntarti Rizqa Febriliany Putri Sadikin, Abdul Halim Saidah Rauf Samsuridjal Djauzi Saptuti Chunaeni, Saptuti Sarsanti, Pungguri Ayu Nega Septelia I. Wanandi Siregar, Fajri Marindra Siti Rahmawati Achyat Sri Sukmaniah Suhendro Suhendro Suradi Susi Rahmiyati Syarifah Dewi Tiara Mayang Pratiwi Lio Tjahjono Darminto Gondhowiardjo Vetnizah Juniantito Wardaya Wardaya Wawaimuli Arozal Wawan Mulyawan Wawan Mulyawan Werdhasari, Asri Wido Sari Nawangsih, Idah Wiji Lestari Wiwoho, Yudi Yuwono

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