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FORMULASI DAN KARAKTERISASI NANOENKAPSULASI YEAST BERAS HITAM DENGAN METODE SONIKASI MENGGUNAKAN POLOXAMER Nur Aida Amyliana; Rudiana Agustini
Unesa Journal of Chemistry Vol 10 No 2 (2021)
Publisher : Department of Chemistry, Faculty of Mathematics and Natural Sciences, Surabaya State University, located at Jl Ketintang, Surabaya, East Java, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (322.474 KB) | DOI: 10.26740/ujc.v10n2.p184-191

Abstract

Abstrak. Yeast beras hitam adalah suatu produk fermentasi pada media tepung beras hitam yang berpotensi untuk dimanfaatkan sebagai senyawa aktif dalam obat. Komponen biokatif dalam yeast perlu dilindungi agar tidak terjadi kerusakan, salah satunya yakni dengan enkapsulasi. Tujuan dari penelitian ini adalah untuk mengetahui pengaruh formulasi dalam nanoenkapsulasi yeast dengan teknik sonikasi yang menggunakan bahan pelindung atau wall material poloxamer. Karakterisasi nanokapsul yang dilakukan meliputi ukuran partikel dan zeta potensial menggunakan alat Particle Size Analyzer ukuran partikel dan pengamatan morfologi partikel menggunakan Transmission Electron Microscopy (TEM). Hasil penelitian menunjukkan bahwa formula terbaik dalam pembuatan nanokapsul yeast adalah dengan perbandingan yeast dan poloxamer sebesar 1:4. Karakterisasi nanokapsul menghasilkan ukuran partikel 260 nm dengan zeta potensial -6.96 mV. Berdasarkan hasil penelitian terlihat bahwa nanokapsul berbentuk partikel yang memanjang dan terikat satu sama lain, secara keseluruhan yeast telah terenkapsulasi oleh poloxamer. Kata kunci : yeast, poloxamer, formulasi, nanoenkapsulasi, sonikasi Abstract. Yeast is a fermentation product in black rice that has opportunity to be used as an active compound in medications. Yeast’s biocative components needed to prevent the damage by encapsulation. The aim of the research was to determine the effect of formulation on nanocapsule yeast using the sonication technique with poloxamer as wall material. Characterization of nanocapsule was done through particle size and zeta potential with the Particle Size Analyzer and particle morphology with Transmission Electron Microscopy (TEM). The results showed that the best formula of yeast nanocapsules at the ratio between yeast and poloxamer was 1: 4. Nanocapsule characterization resulted particle size was 260 nm and a zeta potential was -6.96 mV. Based on the result of the studies, it appears that nanocapsule had elongated morphology that bound to each another, overall the yeast has been encapsulated by poloxamer. Key words: yeast, poloxamer, formulation, nanoencapsulation, sonication
PENENTUAN KANDUNGAN TOTAL ANTOSIANIN YEAST BERAS HITAM (Oryza sativa L. Indica) MENGGUNAKAN METODE pH DIFFERENSIAL Fatimatuz Zahroh; Rudiana Agustini
Unesa Journal of Chemistry Vol 10 No 2 (2021)
Publisher : Department of Chemistry, Faculty of Mathematics and Natural Sciences, Surabaya State University, located at Jl Ketintang, Surabaya, East Java, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (422.021 KB) | DOI: 10.26740/ujc.v10n2.p200-208

Abstract

Abstrak. Beras hitam memiliki berbagai kandungan komponen bioaktif yang berperan sebagai antioksidan meliputi senyawa fitokimia dan antosianin. Kandungan pigmen atau zat warna pada beras hitam disebabkan oleh senyawa antosianin dan proantosianidin. Salah satu produk diversifikasi berbahan dasar beras hitam yang dapat dikembangkan adalah fermentasi menggunakan ragi roti (bakery’s yeast). Penelitian ini bertujuan untuk mengidentifikasi yeast beras hitam sebagai antioksidan dalam senyawa fitokimia dengan Liquid Chromatography Mass Spectrometry (LC-MS), serta mengetahui kestabilan kandungan total antosianin dari berbagai pH asam menggunakan metode pH differensial. Metode ekstraksi yang digunakan adalah maserasi dengan larutan etanol 96% ditambah pelarut HCl 1% hingga pH (1, 1,5, 2, dan 2,5) yang dilakukan selama 24 jam. Hasil penelitian menunjukkan bahwa yeast beras hitam dapat dimanfaatkan sebagai sumber antioksidan yang baik, dikarenakan mengandung 20 senyawa turunan fitokimia dan antosianin yang tinggi. Kandungan total antosianin yeast beras hitam pada variasi pH 1 sebesar 60,132 mg/100g, pH 1,5 127,045 mg/100g, pH 2 47,566 mg/100g, dan pH 2,5 29,790 mg/100g. Senyawa antosianin bekerja secara optimum dalam pelarut asam pada pH 1,5, sehingga dapat disimpulkan bahwa pH dapat mempengaruhi stabilitas pigmen antosianin. Kata Kunci :Yeast beras hitam,antosianin, fitokimia, antosianin, pH differensial. Abstract. Black rice contains various bioactive components that act as antioxidants including phytochemical compounds and anthocyanins. The pigment or dye content in black rice is caused by anthocyanin and proanthocyanidin compounds. One of the diversified products made from black rice that can be developed is fermentation using bakery's yeast. This study aims to identify black rice yeast as an antioxidant in phytochemical compounds with Liquid Chromatography Mass Spectrometry (LC-MS), as well as to determine the stability of the total anthocyanin content of various acidic pH using differential pH methods. The extraction method used was maceration with 96% ethanol solution plus 1% HCl solvent to pH (1, 1.5, 2, and 2.5) which was carried out for 24 hours. The results showed that black rice yeast can be used as a good source of antioxidants, because it contains 20 high levels of phytochemical and anthocyanin derivatives. The total content of black rice yeast anthocyanin at various pH 1 was 60.132 mg / 100g, pH 1.5 127.045 mg / 100g, pH 2 47.566 mg / 100g, and pH 2.5 29.790 mg / 100g. Anthocyanin compounds work optimally in acidic solvents at pH 1.5, so it can be concluded that pH can affect the stability of anthocyanin pigments. Key Words : Yeast black rice, anthocyanins, phytochemicals, pH differential.
Analysis of Effectiveness PBL-STEM to Improve Student’s Critical Thinking Skills Noera Wahdaniyah; Rudiana Agustini; Tukiran Tukiran
IJORER : International Journal of Recent Educational Research Vol. 4 No. 3 (2023): May
Publisher : Faculty of Teacher Training and Education Muhammadiyah University of Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46245/ijorer.v4i3.312

Abstract

Objective: This study aims to analyze the effectiveness of PBL-STEM in improving students' critical thinking skills. Method: The method used in this research is the literature review method. A literature review is a type of research that collects data and information from various sources by collecting and analyzing. This study analyzes as many as two national and international articles that can be accounted for. The articles used were published in 2018-2022. The steps in this Literature review method are identifying topics, searching for and selecting relevant articles, analyzing and synthesizing the literature, and creating a conclusion. Results: (1) the application of the PBL model can improve students' critical thinking skills; (2) the integration of STEM in the learning process is also able to improve critical thinking skills because STEM provides opportunities for students to identify real-life problems and solve them, which is per the characteristics of PBL; (3) the integration between PBL and STEM can be applied and can provide more effective results in improving students' critical thinking skills. Novelty: ThisThis study discusses the analysis of how effective the integration of STEM and PBL from the latest journals so that this research can be used as a reference for making quality learning tools.
Analysis of Effectiveness Argument-Driven Inquiry to Improve Students’ Argumentation Skill and Conceptual Understanding Agustina Dua Kuki; Rudiana Agustini; Utiya Azizah
IJORER : International Journal of Recent Educational Research Vol. 4 No. 3 (2023): May
Publisher : Faculty of Teacher Training and Education Muhammadiyah University of Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46245/ijorer.v4i3.316

Abstract

Objective: This study examined the effectiveness of the Argument-Driven Inquiry learning model in science learning in improving students' argumentation skills and conceptual understanding. Method: The techniques used are 1) searching articles on Scopus and Google Scholar using the keyword Argument-Driven Inquiry in science learning, 2) articles were selected focus on increasing conceptual understanding and argumentation skills, 3) the metadata was selected limited to 2015-2023, and 4) conducting the in-depth review. Results: The ADI model was able to improve students' argumentation skills. In general, students' argumentation levels are in levels 3-4. The quality of the arguments developed by students shows the understanding of the concepts possessed by students. Students can reach the cognitive level created (C6) by writing scientific reports. Novelty: This study reinforces previous research regarding the effectiveness of the ADI model in improving argumentation skills and conceptual understanding taken from recent articles. Therefore, this article can be the basis for developing learning tools for the ADI model.
Isolation and identification of proteolytic lactic acid bacteria from freshwater pomfret (Colossoma macropomum) intestine Risti Wulaningtyas; Rudiana Agustini
Jurnal Pijar Mipa Vol. 18 No. 5 (2023): September 2023
Publisher : Department of Mathematics and Science Education, Faculty of Teacher Training and Education, University of Mataram. Jurnal Pijar MIPA colaborates with Perkumpulan Pendidik IPA Indonesia Wilayah Nusa Tenggara Barat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jpm.v18i5.5481

Abstract

The production of freshwater pomfret (Colossoma macropomum) in Indonesia is abundant, but the digestive tract waste weighing 10-15% of the fish body is not widely utilized. The fish's digestive tract can potentially produce lactic acid bacteria (LAB). Lactic acid bacteria have a proteolytic system that can produce extracellular protease enzymes. Protease enzymes are the most widely utilized enzymes in various industries and have a global revenue of up to $2.1 billion in 2021. Protease enzymes derived from microbes are in great demand due to the efficiency of time, place, cost, and better cultivation techniques than animal and plant sources. This study aimed to isolate and identify proteolytic lactic acid bacteria from the intestines of freshwater pomfret fish and determine their proteolytic index. The methods used were morphological, biochemical, sugar, and proteolytic tests. The BAT-A isolate with the highest proteolytic index of 2.52 with characteristics of Gram-positive, non-spore, facultatively anaerobic, catalase-negative, non-motile, homofermentative, can ferment glucose, maltose, lactose and sucrose which were then identified as proteolytic lactic acid bacteria Enterococcus sp which has the potential as a candidate source of protease enzymes from bacteria isolated from the intestines of freshwater pomfret.
Effectiveness of ADI-STEM to Improve Student’s Science Literacy Skill Sigit Purnomo; Yuni Sari Rahayu; Rudiana Agustini
IJORER : International Journal of Recent Educational Research Vol. 4 No. 5 (2023): September
Publisher : Faculty of Teacher Training and Education Muhammadiyah University of Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46245/ijorer.v4i5.382

Abstract

Objective: This study aims to analyze the effectiveness of the Argument-Driven Inquiry (ADI)–Science, Technology, Engineering, and Mathematics (STEM) model in improving students' scientific literacy abilities. Method: The method used in this research is the literature review method. The literature review is a type of research used to collect data and information by collecting and reviewing various references. This study analyzes as many as 20 national and international articles that can be accounted for. The articles used were published in 2015-2022. The steps taken in this study were identifying topics, finding and selecting appropriate articles, analyzing and synthesizing the literature, and concluding. Result: Based on the results of the studies and analyses that have been carried out, it can be concluded that (1) the application of the ADI learning model can improve students' scientific literacy skills; (2) the ability to argumentation skills to increase students' scientific literacy; (3) integrating STEM into the learning process is also able to improve scientific literacy skills because STEM provides opportunities for students to identify real-life problems. Novelty: This study reveals that students' scientific literacy can be increased effectively by integrating ADI and STEM. These findings invite researchers, educators, and the government to develop learning that facilitates ADI-STEM learning.
Development of Website-Based Learning Media on Reaction Rate Material to Improve Student Learning Outcomes Evita Harti Nanda; Rudiana Agustini
Hydrogen: Jurnal Kependidikan Kimia Vol 11, No 4 (2023): August 2023
Publisher : Universitas Pendidikan Mandalika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/hjkk.v11i4.8523

Abstract

This development research aims to describe the feasibility of website-based learning media for reaction-rate material. The feasibility of learning media is reviewed in terms of validity, practicality, and effectiveness. Media validity is measured by content validity and construct validity. The practicality of the media is measured by student responses and activities. Effectiveness is measured by the percentage of classical completeness and the improvement of students' learning outcomes. The research method used in this research is Borg and Gall's Research and Development (R&D) method, which has been modified by Sukmadinata. Media trials were conducted at SMA Negeri 20 Surabaya on 33 students of class XI MIPA 2 who had previously received reaction rate material. This website-based learning medium is declared feasible. The results of content and construct validity show valid results in each aspect with a mode between 4 and 5. Practicality results show that web-based learning media is practical with 98,10% of relevant student activities at the first meeting and 99,05% at the second meeting, while the percentage acquisition of each aspect in the student response gets a percentage of  90%. The results of the effectiveness of learning media obtained classical completeness results of 87.9% and an N-Gain score of 0.78. This is supported by the results of the Paired sample t-test of 0.000, which means there is a significant average difference in learning outcomes.
Development of Android-Based Marbles Chem Hydrocarbon Mobile Game Media to Improve Motivation and Learning Outcomes of 11th Grade Science Students Izzatul Muhidah; Rudiana Agustini
Hydrogen: Jurnal Kependidikan Kimia Vol 11, No 6 (2023): December 2023
Publisher : Universitas Pendidikan Mandalika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/hjkk.v11i6.8728

Abstract

One of the uses of ICT in the world of education can be in the form of game media because students are actively involved in learning so that it can increase motivation. Learning outcomes are related to motivation, if the motivation given is more appropriate, the more successful the learning will be. This study aims to obtain feasible Marbles Chem Hydrocarbon media to increase student motivation and learning outcomes based on three aspects, namely validity, practicality, and effectiveness. The research method used is research and development / Research and Development (R&D) adaptation from Plomp (2013), consisting of three phases, namely the Preliminer Phase, the Prototype Making Phase, and the Evaluation Phase. Limited trials were conducted in 11th Grade Science (MIPA) of State Senior High School (SMAN) 20 Surabaya. Validity is obtained from three validators. Practicality is obtained from the results of response questionnaires, observation of student activities, and the implementation of learning. effectiveness is obtained from ARCS motivation questionnaire sheets and student learning outcomes. Media validity on content validity and construct validity barada in the range of scores 4 and 5 with valid and very valid criteria. Practicality is reviewed based on observations of learning implementation, student response questionnaires, and observations of student activities with an average percentage of 98.47%, 96.98%, and the results of observations of relevant activities are greater than irrelevant activities, namely 205.16 > 6.43. The effectiveness of the media obtained pretest-posttest increased from 60 incomplete categories to 87.69 complete categories and the ARCS motivational questionnaire increased from 55.32 with the moderately effective category to 95.85 very effective categories. Based on the results of validity, practicality and effectiveness, Marbles Chem Hydrocarbon media is suitable to be used as a learning medium on Hydrocarbon material.
PELATIHAN PENYUSUNAN SOAL BERBASIS ARGUMENTASI BAGI GURU KIMIA DI KABUPATEN GRESIK Rusmini, Rusmini; Agustini, Rudiana; Nasrudin, Harun; Muchlis, Muchlis; Dwiningsih, Kusumawati; Hidayah, Rusly
Jurnal ABDI: Media Pengabdian Kepada Masyarakat Vol. 9 No. 2 (2024): JURNAL ABDI : Media Pengabdian Kepada masyarakat
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/abdi.v9i2.28686

Abstract

Telah dilaksanakan kegiatan pengabdian kepada Masyarakat (PKM) untuk guru kimia MGMP Kabupaten Gresik. Tujuan kegiatan PKM ini adalah melatihkan penyusunan soal berbasis argumentasi. Hal ini dikarenakan berdasarkan hasil angket pra PKM menunjukkan guru belum melakukan penilaian pada keterampilan argumentasi peserta didik. Kegiatan PKM dilakukan mulai tahap persiapan, pelaksanaan dan pelaporan. Hasil pelaksanaan pelatihan menunjukkan bahwa guru-guru peserta pelatihan memberikan respon yang sangat baik terhadap kegiatan pelatihan. Peningkatan kemampuan argumentasi berdasarkan hasil pretes postes dengan perkembangan rendah 15,385%, sedang 23,077% dan tinggi 61,538%. Kemampuan guru dalam menyusun soal berbasis keterampilan argumentasi telah tercapai 100%. Kegiatan ini menjadi Langkah awal bagi para guru untuk melatihkan lebih lanjut ke peserta didiknya mengingat pentingnya kemampuan argumentasi ini
Development of Student Worksheets based Assessment for Learning on Chemical Equilibrium to Improve Student Science Process Skills Utami, Dianita Fitri; Agustini, Rudiana
Hydrogen: Jurnal Kependidikan Kimia Vol 12, No 4 (2024): August 2024
Publisher : Universitas Pendidikan Mandalika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/hjkk.v12i4.12617

Abstract

This study focuses on creating Student Worksheets (LKPD) that incorporate Assessment for Learning principles to improve students' scientific process skills in chemical equilibrium. The research employs the ADDIE (Analysis, Design, Development, Implementation, Evaluation) model of Research and Development (R&D). The study evaluates the LKPD's effectiveness, practicality, and validity. A one-sample t-test comparing pretest and posttest scores measures effectiveness. Practicality is determined through quantitative descriptive analysis of student feedback questionnaires and activity observation sheets. Validity assessment involves both qualitative and quantitative descriptive analysis using Likert scale validation forms. Results show that the developed LKPD achieved the highest possible validity score (mode of 5) across all aspects. It demonstrated 90.7% practicality and a statistically significant t-test result (p-value of 0.000). These outcomes suggest that the LKPD effectively enhances students' scientific process skills in chemical equilibrium studies. The Assessment for Learning approach and integrated feedback in the LKPD promote active student engagement and foster improved scientific process skills in this subject area.
Co-Authors Adyani, Laely Afian, Tilal Afifah, Diah Siti Agustyna Widyaningrum Ahlan Riwahyu Habibi Aida Fikriyah Alberto Yonathan Tangke Allo Andriani, Lupita ANTIKA SARI, VIVIN Arviana Ramadhanti Asri Susetyo Rukmi BENI SETIAWAN Budi Jatmiko Budi Jatmiko Cahyani, Agis Ayu Dewi Markiah DIAN ERLITA PUTRI, NAZTITI Dian Pradianti Dr. I Nyoman Tika,M.Si . Eka Septianingtyas Elok Sudibyo Ely Rohmawati Ely Rohmawati Endah Rahmawati ERMAN ERMAN Erman Erman Evita Harti Nanda Fatimatuz Zahroh Fauziyah Nisyah Feliatra Findiyani Ernawati Asih Firdaus, Laras Fitria Hidayati Fuaidah, Nur Gea, Natasyah Valencia Nilafah Harun Nasrudin Harun Nasrudin Hasanah, Kholifia Nabila Hasnawati Hasnawati Hasnawati Hasnawati Hendra Eka Nurdyanto Hiatun Sri Wahyuni Hurint, Jansensius Dani I Gusti Agung Komang Diafari Djuni Hartawan I Gusti Ayu Tri Agustiana Ibrahim, Musimin Imran, Yuliana Anggraini Izzatul Muhidah Jansensius Dani Hurint Julianto Julianto Julianto Julianto Julianto Julianto Kuki, Agustina Dua Kusumawati Dwiningsih Laely Adyani Lamdari, Eka Yuniar Retno Laras Firdaus Lathifatul Hikmah Layyina, Nunik Luthfi Faza Afina Riza Walida Luthfi Faza Afina Riza Walida, Luthfi Madlazim Madlazim Madlazim Madlazim Maghfiroh, Lailatul Mahyuddin Syaifulloh Markiah, Dewi Mu'minatus Solekha, Fadia Muchlis Muchlis Muchlis Muchlis Muchlis Muchlis Muh Makhrus Musimin Ibrahim MUSLIMIN IBRAHIM N Narsito NABILAH, SALMA Narsito Narsito Narsito Narsito Noera Wahdaniyah Nofi Eriana Sulistiani Nunik Layyina Nur Aida Amyliana NUR ALIZA AGUSTINA, SAVIRA Nur Fuaidah Nurdyanto, Hendra Eka Nurhayati Nurhayati Nurhikma Ramadhana Nurlarasati, Filla Qodaria Oktavilani, Nabila Defti Pradianti, Dian PRATIKNO, PUTRI PUJI ASTUTIK, SETYO Purwono, Tri Diansy Yana Putra, Armansyah QODARIA NURLARASATI, FILLA Raharjo Raharjo Raharjo Raharjo Rika Dyanita Sari Risti Wulaningtyas RIZKI, ULFATIKHAH Rizza Danila Rofiqotus Sholeha RR. Ella Evrita Hestiandari RUSLY HIDAYAH Rusmini Rusmini S.Pd. M Kes I Ketut Sudiana . Sari Edi Cahyaningrum Sari, Ilma Indana Satriawan, Muhammad Setiawan, Azzahra Laili Tivalia Putri SHOLIHA, AMILATU Sifak Indana Sigit Purnomo Soda, Fransiska Nay Sri Juari Santoso Sri Juari Santoso Sri Juari Santoso Sri Martini Sri Martini Sri Poedjiastoeti Suhartini, Erna Supardi, ZA Imam Suprayitno Suprayitno Surastri, Surastri SUYATNO Suyono Suyono Tangke Allo, Alberto Yonathan TAOFEK, IWAN Tirsa Fitriana Angsari Titik Taufikurohmah Titik Taufikurohmah Tjandrakirana Tjandrakirana Tjipto Prastowo Tjipto Prastowo Toeti Koestiari TOETI KOESTIARI Toeti Koestiari Tukiran Tukiran Tukiran Tukiran Tukiran Tukiran Utami, Dianita Fitri Utiya Azizah Wahono Widodo Wahono Widodo Wahono Widodo Wahono Widodo Wahyuni, Hiatun Sri Wakhidah, Nur Wasis Wasis Wasis Wasis Wasis WIDOWATI BUDIJASTUTI Widowati, Azza Nuriah Wihdatul Istiqah Yuliana Anggraini Imran Yuni Sari Rahayu YUNI SRI RAHAYU Yunita Yunita Yunita Yunita Z. A. Imam Supardi Z. A. Imam Supardi ZA Imam Supardi Zahro, Latifatuz