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All Journal Seminar Nasional Aplikasi Teknologi Informasi (SNATI) JURNAL SAINS PERTANIAN EQUATOR Jurnal Pendidikan dan Pembelajaran Khatulistiwa (JPPK) Jurnal Kelautan : Indonesian Journal of Marine Science and Technology Jurnal Online Mahasiswa (JOM) Bidang Teknik dan Sains Jurnal Kelautan Tropis Omni-Akuatika Jurnal Universitas Paramadina Loquen: English Studies Journal Martabe : Jurnal Pengabdian Kepada Masyarakat Jurnal Kelautan Nasional Abdi Dosen : Jurnal Pengabdian Pada Masyarakat Jurnal Ilmiah Profesi Pendidikan Jurnal Riset Jakarta Jurnal Abdi Insani Jurnal Chart Datum Community Development Journal: Jurnal Pengabdian Masyarakat Jurnal Syntax Imperatif : Jurnal Ilmu Sosial dan Pendidikan Journal of Social Research COMSERVA: Jurnal Penelitian dan Pengabdian Masyarakat Journal Of Human And Education (JAHE) Indonesian Andrology and Biomedical Journal TRANSPUBLIKA INTERNATIONAL RESEARCH IN EXACT SCIENCES (TIRES) Integrasi : Jurnal Ilmiah Teknik Industri VISA: Journal of Vision and Ideas Journal of Education Sciences (Edusci) GENEVA: Jurnal Teologi dan Misi Folia Medica Indonesiana
Agustinus Agustinus
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Impact of preparation using conventional and modified density gradient centrifugation methods on sperm concentration, motility and number of normal motile sperm recovery (nmsr) Yudiwati, Rina; Pramesti, MPBD; Agustinus, Agustinus; Pradana, E; Purwanto, Bambang
Folia Medica Indonesiana Vol. 53, No. 3
Publisher : Folia Medica Indonesiana

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Abstract

Some preparation techniques, either conventional or advanced, have been provided. Advanced technique may overcome the limitations of conventional techniques. Recently, not all fertility clinics in Indonesia are able to provide advanced preparation techniques. Some techniques require expensive equipments and can only be used for intracytoplasmic sperm injection (ICSI). Some fertility clinics use a modified procedure, namely the combination of density gradient centrifugation with a swim-up method for the preparation of the sperm to be used in ART. This study aimed to determine whether the modified density gradient centrifugation, which is density gradient centrifugation followed by a swim-up, is able to yield better results than conventional density gradient centrifugation. This study was a laboratory experimental pre and pos-test control group design. Population was all adult men aged 21-40 years old and the sampling unit was the man donor's semen which fulfilled inclusion criterias, collected during the periode of the study. Sample size was eight. Sperm analysis were done before and after preparation in conventional and modified group. Descriptive comparation analysis have been used. This study obtained NMSR 7.9+5.5 million/ejaculate and recovery rate (RR) 27.66+11.8 %. RR was lower compared to RR obtained conventional DGC method. RR might be lower because in modified DGC samples undergo two steps selection while conventional DGC only one step selection. But conventional DGC samples should be centrifuged twice, therefore sperms might experience more trauma. Lower RR sperm does not exclude the possibility to be used for ART, because still within the required number for all TRBs. In conclusion, modified DGC preparation method obtained lower NMSR and RR, nevertheless harvested sperms can still be used in all kind of ART.
Co-Authors A. Wanaputra, Amadion Adi Chandra Adi Suyatno Adriyanto, Agus Aldri Frinaldi Alfonsus, Alfonsus Allo, Elisabet Payung Allo, Elisabeth Payung Anak Agung Gede Sugianthara Andi Indriani Ibrahim Angellee, Jesselyn Apriyanto, I Nengah Putra Arfianty Arfianty, Arfianty Asmoro, Nuki Widi Aucky Hinting Azhari, Ferian Bambang Purwanto Budi Purwanto Choirul Umam, Choirul Ditto Rezkiawan Dwi, Dwi Eliana Sari, Eliana Elis, Elis Timang Enos Tambing Fajriawan, Rezki Femi Dwi Astuti Harnida Wahyuni Adda Hartanto, Markus Christian HASAN MADANI Henry Munandar Manik Hery Kresnadi I Made Jiwa Astika I'tishom, Reny Irwanto, Bambang Joni Karnati, Neti Kurniawan , Muhammad Aziz Kurniawan, Muhammad Azis La Ode Musa Lapituley, Frank Lionardi, Samuel Mangalo, David Mastar Asran Maulana Ishom Putra, Wildan Mega Fatimah Rosana Melka Parmanto, Cristian Muharrom, Eka Yudha Narulita, Pety Nurhajati Nurhajati Nurwani Nurwani, Nurwani Payung Allo, Elisabeth Petrus Kune Pradana, E Pramesti, MPBD Pricylia Chintya Dewi Buntuang Putri Handini, Lika R. Riwu, R. Riwu R., Agustina Rahmatullah, Amri Regina Retnowati, Anis RINA YUDIWATI BM, RINA Rosadi, Imran Rudy Rudy Sadiyah, Alifatus Setiono, Budi Setyono, Vicky Indarto Siswahyudianto Sufianto, Permana Suharyani, Sally Sukoco, Nawanto Budi Sulle, Yosef Sulton, Muhammad Supriyanto, Taufiq Susanto, Natasha Susilo, Rahadian Indarto Syafri, Syafri Syahrizal Pasaribu, Wanly Theresia Sunarni Thomas Pagasis Tri Aji Triono, Yoyok Uly, Kirenius Wardah Widodo S Pranowo William William Wiri Wirastuti Y. Jelita, Marina Zara Elisabeth Tanring Zerlinda, Ivana