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DNA Barcoding of Red Algae (Rhodophyta) in Ternate Island Sea, North Maluku, Indonesia Achmad, Muhammad Janib; Akbar, Nebuchadnezzar; Ismail, Firdaut; Samman, Ardan; Subhan, Beginer; E Paembonan, Rustam; Arafat, Dondy
Jurnal Ilmiah Perikanan dan Kelautan Vol. 16 No. 1 (2024): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v16i1.44436

Abstract

Abstract Ternate Island is located on the North Maluku Sea. The North Maluku Sea region includes the Wallacea area and the world's coral triangle. One of the organisms commonly found in this sea is red algae. Study aimed to determine the red algae species by phylogenetic tree analysis based on the rbcL gene as a DNA marker. The preserved red algae tissue samples were extracted with the Geneaid GP100 DNA Extraction Kit Plant. The DNA sample was amplified and then visualized by 1% agarose gel electrophoresis. The amplicon products were sequenced and then aligned with the rbcL gene database that was available at the NCBI gene bank. The phylogenetic tree was constructed using the UPGMA method. The results showed that red algae were identified into four species: namely Gibsmithia hawaiiensis (98.65%), C_rbcL sample was identical to Amansieae sp. (91.50%), D_rbcL sample was identical to Peyssonnelia sp. (95.54%), and G_rbcL sample was similar to Portieria hornemanniI (96.15%). Based on phylogenetic tree analysis, Gibsmithia hawaiiensis from North Maluku is closely related to species from Raja Ampat, West Papua, Indonesia, followed by the Philippines species. Special findings were found to carry out phylogenetic reconstructions that can answer inter-species kinship. The general finding is that the markers used can be used for phylogenetic construction. Phylogenetic construction of Peyssonnelia sp. in North Maluku is related to species from South Africa. North Maluku's Portieria hornemannii is closely related to a species from Korea. Highlight Research This study provides initial information about the DNA barcoding of red algae. Important for management purposes and determining the status of red algae in the future. This scientific information also provides an understanding of the relationships among red algae species in the world's oceans. Global warming has changed the temperature of seawater and affected the population of marine organisms.
Ekologi dan Dimensi Pengelolaan Keberlanjutan Mangrove (Studi Kasus Lokasi Ekowisata Guraping, Kota Tidore Kepulauan, Maluku Utara) Paembonan, Rustam Effendi; Akbar, Nebuchadnezzar; Ibrahim, Abjan; Tahir, Irmalita; Baksir, Abdurrachman; Marus, Ikbal; Najamuddin, Najamuddin; Ismail, Firdaut; Wibowo, Eko S; Siolimbona, Abdul Ajiz; Harahap, Zulhan Arifin; Mutmainnah, Mutmainnah; Angkotasan, Abdul Motalib; Zamani, Neviaty P; Arafat, Dondy; Subhan, Beginer; Natih, Nyoman MN; Rahman, Rahman; Rahimah, Insaniah; Harahap, Zulham Apandy
Jurnal Sumberdaya Akuatik Indopasifik Vol 8 No 4 (2024): November
Publisher : Fakultas Perikanan dan Ilmu Kelautan, Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46252/jsai-fpik-unipa.2024.Vol.8.No.4.412

Abstract

The mangrove ecosystem is a potential coastal and marine area that has a very important resource function. The mangrove ecosystem must be managed sustainably so that it can provide optimal benefits, especially the mangrove ecosystem in Guraping Village which has been designated as an ecotourism area. The aim of this research is to analyze the ecological condition of the mangrove ecosystem and analyze the dimensions of sustainable management of the mangrove ecosystem in Guraping Village. The method used in this research line transect method with a 10x10 meter quadrant to determine ecological conditions. Interviews were conducted with key respondents from stakeholders and the dimensions of sustainable management were analyzed using Rapfish. Measurement of environmental parameters directly on site. The results obtained from this research can determine the ecological condition of mangroves at the research location which still have good status based on the number of species, species density, species cover, diversity index, mangrove canopy cover data. The calculation of the results of the assessment of the dimensions of mangrove sustainability management, which consists of ecological dimensions, economic dimensions, socio-cultural dimensions, legal, institutional and policy dimensions and technological and infrastructure dimensions, is classified as a less accountable level category.
Potensi aktivitas antioksidan ekstrak spons laut Stylissa carteri: Potential antioxidant activity of sea sponge Stylissa carteri extract Adewal, Murad Alvian K; Abdullah, Asadatun; Zamani, Neviaty Putri; Arafat, Dondy; Ayu, Inna Puspa; Subhan, Beginer
Jurnal Pengolahan Hasil Perikanan Indonesia Vol. 28 No. 3 (2025): Jurnal Pengolahan Hasil Perikanan Indonesia 28(3)
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17844/jphpi.v28i3.60270

Abstract

The sea sponge Stylissa carteri plays an important role because it is one of the constituent biota of coastal and marine ecosystems, especially in coral reef ecosystems. The increasing utilization of bioactive compounds in sea sponges is feared to significantly reduce the population of sea sponges. The prevention effort involves cultivating sea sponges for sustainable utilization. This study aims to determine the antioxidant activity of transplanted Stylissa carteri sponges and those that live naturally in nature. This study was conducted in Pramuka Island using the sponge explant combination method. The three types of transplants were number one (SO), number two (S. carteri and Aaptos suberitoides), and number three (S. carteri, A. suberitoides, and Acropora hard coral). Antioxidant activity was tested with three repetitions on three transplanted and naturally occurring S. carteri sponges (SA) using the DPPH (1,1-Diphenyl-2-Picrylhydrazyl) method. The results indicated that the four sample sources contained strong antioxidant activity, namely SO had an IC50 value of 86.22±23.6 μg/mL, SAP 86.58±30.7 μg/mL, SAC 86.10±23.4 μg/mL, and natural living SA 54.27±16.9 μg/mL. The transplanted S. carteri sea sponge has strong antioxidant activity and can be an alternative solution in terms of raw material availability for the development of potential pharmaceutical products.
POLA KEANEKARAGAMAN DAN DISTRIBUSI SPESIES IKAN TERUMBU MENGGUNAKAN ENVIRONMENTAL DNA (eDNA) METABARCODING PADA JARAK LONGITUDINAL DARI TELUK JAKARTA Sani, Lalu M Iqbal; Benyamin, Azhari; Husna, Alief K; Arafat, Dondy; Subhan, Beginer; Sunuddin, Adriani; Cakasana, Nadya; Lestari, Dea Fauzia; Madduppa, Dr. Hawis
Jurnal Ilmu dan Teknologi Kelautan Tropis Vol. 13 No. 3 (2021): Jurnal Ilmu dan Teknologi Kelautan Tropis
Publisher : Department of Marine Science and Technology, Faculty of Fisheries and Marine Science, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jitkt.v13i3.37971

Abstract

Keberadaan ikan terumbu tentunya erat kaitannya dengan keberadaan terumbu karang karena ekosistem tersebut merupakan habitat bagi ikan karang. Terumbu karang merupakan ekosistem yang banyak terdapat di pulau-pulau kecil di daerah tropis termasuk Kepulauan Seribu. Kepulauan Seribu adalah sekelompok 110 pulau yang terletak di lepas pantai Jakarta dan hingga 80 kilometer sebelah utara Laut Jawa. Dalam studi ini, kami meneliti distribusi spesies dan keanekaragaman spesies ikan terumbu pada dua lokasi yaitu Pulau Untung Jawa dan Pulau Harapan yang berbeda jarak di Teluk Jakarta menggunakan analisis metabarcoding DNA lingkungan (eDNA). Sebanyak 4 liter sampel air laut dikoleksi pada kedalaman 8-9 meter per lokasi lalu dilakukan analisis menggunakan primer spesifik (MiFish U) dengan marka 12s rRNA. Secara keseluruhan, kekayaan spesies yang lebih tinggi ditemukan di Pulau Harapan (52 spesies) diikuti oleh Pulau Untung Jawa (11 spesies). Indeks Shanon-Wiener juga menunjukkan bahwa Pulau Harapan memiliki keanekaragaman ikan terumbu yang lebih tinggi berdasarkan tiga tingkatan taksonomi (famili, genus, dan spesies). Hanya ada lima spesies ikan terumbu mutual yang ditemukan di dua lokasi tersebut, yakni Atherinomorus aetholepis, Auxis thazard, Cephalopholis sexmaculata, Epinephelus chlorostigma, dan Plectropomus areolatus. Hasil temuan dalam penelitian ini sejalan dengan perbedaan antropogenik dimana Pulau Untung Jawa lebih dekat dengan Teluk Jakarta dibandingkan dengan Pulau Harapan yang letaknya relatif jauh dari Teluk Jakarta.
Mengungkap Aspek Biologi dan Emergence Point Hiu Berjalan Halmahera (Hemiscyllium halmahera) di Laut Pulau Maitara Maluku Utara Indonesia Akbar, Nebuchadnezzar; Ismail, Firdaut; Siolimbona, Abdul Ajiz; Tahir, Irmalita; Paembonan, Rustam E; Subhan, Beginer; Arafat, Dondy; Baksir, Abdurrachman; Subur, Riyadi; N, Furqan; Yidoatimojo, Sudibyo; Nurjaya, I Wayan; Restu, Yunan Gilang; N Natih, Nyoman Metta; Hari, Restu Fajar; Bengen, Dietriech G.
Jurnal Kelautan Tropis Vol 28, No 3 (2025): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkt.v28i3.28334

Abstract

The Halmahera Walking Shark (Hemiscyllium halmahera) is an endemic marine biota in the Halmahera Sea. This species has a local migration pattern and is territorial. The distribution of this species is in the intertidal area to a depth of 10-15 meters. Previous studies have shown that this species is spread across several islands in the North Maluku Islands. Maitara Island is a small island located between the North Maluku archipelago. The Halmahera Walking Shark species is also found on this island. Information on the Halmahera Walking Shark species is not fully documented. The purpose of the study is related to biological information and emergence points (emergence points) in the Maitara Island Sea. Data collection was carried out on coral reefs, seagrass and mangroves. Samples were captured and morphometric measurements, weight and sex observations were taken. The sea area where the species was found was used to obtain coordinates for the emergence point. An analysis of length-weight relationship data was carried out to see growth patterns. The study found that morphometric measurements varied among individuals, although there were general similarities. Sexes were found in pairs (Male and Female), indicating that proportions and mating were still normal in nature. The habitat of this species is found in the mangrove area, coral reefs, and seagrass, thus describing that there is an association in nature. The emergence point shows that the Halmahera Walking Shark is found along the coast of Maitara Island although the frequency of the number is different. The length-weight relationship found a negative allometric growth pattern. Information on biological aspects and emergence points is important, to be used as a reference for migration areas and habitats for the interests of species, waters, and ecosystem conservation.  Hiu Berjalan Halmahera (Hemiscyllium halmahera) merupakan biota laut endemik di laut Halmahera. Spesies ini memiliki pola migrasi lokal dan bersifat teritorial. Penyebaran spesies ini di wilayah intertidal hingga kedalaman 10-15 meter. Penelitian terdahulu menunjukan bahwa spesies ini tersebar di beberapa pulau di Kepulauan Maluku Utara. Pulau Maitara merupakan pulau kecil yang terletak diantara gugusan kepulauan Maluku Utara. Pulau ini juga ditemukan speises Hiu Berjalan Halmahera. Informasi belum terekam lengkap terkait spesies Hiu Berjalan Halmahera. Tujuan penelitian terkait informasi biologi dan emegence point (titik kemunculan) di Laut Pulau Maitara. Pengambilan data dilakukan di terumbu karang, lamun dan mangrove. Sampel ditangkap dan diambil ukuran morfometrik, berat dan pengamatan kelamin. Area laut ditemukannya spesies diambil koordinat untuk di jadikan sebagai titik kemunculan.  Analisis data hubungan panjang-berat dilakukan untuk melihat pola pertumbuhan. Hasil penelitian ditemukan ukuran morfometrik bervariasi pada setiap individu, meskipun terdapat kemiripan secara umum. Kelamin ditemukan sepasang (Jantan dan Betina), dengan demikian menunjukan proporsi dan perkawinan masih normal di alam. Habitat tertangkap spesies ini di area mangrove, terumbu karang dan lamun, sehingga mendeskripsikan terdapat asosiasi di alam. Titik kemunculan menunjukan bahwa, Hiu Berjalan Halmahera ditemukan di sepanjang pesisir laut Pulau Maitara meskipun frekuensi jumlah berbeda. Hubungan panjang berat menemukan pola pertumbuhan allometrik negatif. Informasi aspek biologi dan emergence point penting, untuk dapat dijadikan rujukan wilayah migrasi dan habitat untuk kepentingan konservasi spesies, perairan dan ekosistem.    
Membangun Indikator Keberhasilan Restorasi Terumbu Karang Subhan, Beginer; Dondy Arafat; Inna Puspa Ayu; Nebuchadnezzar Akbar
Policy Brief Pertanian, Kelautan, dan Biosains Tropika Vol. 7 No. 4 (2025): Policy Brief Pertanian, Kelautan, dan Biosains Tropika
Publisher : Direktorat Kajian Strategis dan Reputasi Akademik IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/agro-maritim.0704.1442-1447

Abstract

Restorasi terumbu karang adalah serangkaian upaya ilmiah dan manajerial untuk memulihkan ekosistem terumbu karang yang rusak akibat faktor alam dan aktivitas manusia. Teknik restorasi yang digunakan termasuk transplantasi karang, pembuatan terumbu karang buatan, dan pengendalian stresor lokal seperti polusi, overfishing, dan perubahan iklim. Kegiatan restorasi di Indonesia telah dilaksanakan di lebih dari 500 titik dalam 30 tahun terakhir, melibatkan ribuan unit terumbu buatan dan jutaan fragmen karang. Namun, banyak dari kegiatan ini tidak menunjukkan keberhasilan yang ditandai dengan kegagalan.  Faktor peningkatan suhu laut, persaingan dengan alga atau spons, serta faktor manusia menjadi penyebab utama. Saat ini, Indonesia belum memiliki standarisasi untuk menilai keberhasilan restorasi terumbu karang. Oleh karena itu, penting untuk mengembangkan parameter dan indikator yang dapat digunakan untuk menilai efektivitas dan keberhasilan kegiatan restorasi, guna memastikan keberlanjutan ekosistem terumbu karang.
Biodiversity and DNA Barcoding of Seagrass in Equatorial Latitudes Regional Representation of Tropical Indonesia Would Improve Clarity, Structure, and Scientific Precision Subur, Riyadi; Akbar, Nebuchadnezzar; Abubakar, Salim; Ismail, Firdaut; Paembonan, Rustam E; Subhan, Beginer; Arafat, Dondy; Irfan, Muhammad
Jurnal Ilmiah Perikanan dan Kelautan 2026: IN PRESS ISSUE (JUST ACCEPTED MANUSCRIPT, 2026)
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v18i2.83915

Abstract

Graphical Abstract Highlight Research 1. This research provides initial and current information regarding seagrass on small islands right on the equator. 2. directly depicts the diversity, density and cover of seagrass in a unique region, namely a small island crossed by the equator. 3. The environmental conditions of marine waters in seagrass areas on islands crossed by the equator have been described. 4. The latest information specifically describing seagrass and the ecology of equatorial marine environments. Essential for the development of future knowledge and technology. 5. DNA barcoding was capable of resolving field identification challenges and providing taxonomic clarification based on DNA sequence data.   Abstract The equator is a hypothetical line that separates the general region from the world.  Complete information on coastal ecosystems has not been provided for seagrass in the equatorial latitudes. Seagrass is dynamically distributed throughout the equatorial latitudes. The area that the equatorial latitudes also traverse is known as the indotropic region. Eastern Indonesia's Kayoa Island is situated directly on the equator.  A problem, namely how is the biodiversity of seagrass in the equatorial region. There are no published research on seagrass, particularly on biodiversity. The aim of this study is to reveal the diversity of seagrass in equatorial latitudes on the small islands. The methodology in this study was purposive sampling with a quadrant line transect was used to obtain the samples.  In situ identification of seagrass species was done.  Seagrass samples were taken from the leaves for DNA analysis purposes. Leaf samples were analyzed molecular in the laboratory. To determine the distribution of seagrass ecology and environmental were subjected to PCA analysis. The results found that six species of seagrass.  It has been discovered that seagrass may grow and develop in the tropical region under typical environmental conditions.  Despite the fact that six species were discovered, diversity was limited.  In this region, seagrass cover is typically high and supports a healthy species density.  In general concluded that diversity and density are low but cover is still quite good. DNA barcoding has revealed differences in morphological identification.This discovery represents preliminary data that can be investigated further in subsequent studies.    
Co-Authors Abdul Motalib Angkotasan Abdurrachman Baksir Abdurrachman Baksir, Abdurrachman Abjan Ibrahim, Abjan Abubakar, Salim Achmad, Muhammad Janib Adewal, Murad Alvian K Aditya Bramandito Adriani Sunuddin Ahmad Taufik Ghozali Ahmad Taufik Ghozali Aisyah, Siti Zanuba Anwar, Muhammad Fathur Aradea Bujana Kusuma Aradea Bujana Kusuma Ardan Samman Asadatun Abdullah Asep Sandra Budiman, Asep Sandra Ayu Ervinia Ayu, Inna Puspa Bashari, Muhammad Hasan Beginer Subhan Benyamin, Azhari Bertoka Fajar S.P. Negara Citra S. U. Dewi Citra Satrya Utama Dewi Dea Fauzia Lestari, Dea Fauzia Dedi Soedharma Dedi Soedharma Denny Khaerudi Dewi, Citra Satrya Utama Dietrich Geoffrey Bengen Dietrich Geoffrey Bengen Dietriech G Bengen Dietriech Geoffrey Bengen E Paembonan, Rustam Elfahmi Elfahmi, Elfahmi Fadhilah Rahmwawati Fadillah Rahmawati Fauzan Dzulfannazhir Fauzan Dzulfannazhir Febriantika, Putri Firdaut Ismail Genadi Algadri Hari, Restu Fajar Hawis H Madduppa Husna, Alief K I Wayan Nurjaya Ikbal Marus, Ikbal Inna Puspa Ayu Irmalita Tahir Irmalita Tahir, Irmalita Ismail, Firdaut Khairudi, Denny Kotta, Raismin Lalu M. Iqbal Sani Lalu M. Iqbal Sani Luzmi Malia Izza Muhammad Irfan Muhammad Janib Achmad Mutmainnah Mutmainnah N Natih, Nyoman Metta N, Furqan Nadya Cakasana Najamuddin Najamuddin, Najamuddin Nebuchadnezzar Akbar Nebuchadnezzar Akbar NEVIATY PUTRI ZAMANI Ni Kadek Dita Cahyani, Ni Kadek Dita Novriyandi Hanif Nur Ari Bayu Nurlita Putri Anggraini Nurlita Putri Anggraini Nyoman M N Natih Paembonan, Rustam E Prakas Santoso Prakas Santoso Prakas Santoso Prehadi . Prehadi Prehadi Rahimah, Insaniah Rahman Rahman Rani Utari Ayuningtyas Rasidi, Rasidi Restu, Yunan Gilang Riyadi Subur, Riyadi Rustam E Paembonan Rustam Effendi Paembonan, Rustam Effendi Sani, Lalu M Iqbal Sani, Lalu M. Iqbal Sebastian C. A. Ferse Sebastian Ferse Setiamarga, Davin H. E. Siolimbona, Abdul Ajiz Sukandar Sukandar Sukandar, Mr. Syafrizayanti, Syafrizayanti Wahab, Iswandi Wibowo, Eko S Wibowo, Eko Setyobudi Yidoatimojo, Sudibyo Yosie Andriani Zulham Apandy Harahap Zulhan Arifin Harahap