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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Buletin Agrohorti Jurnal Fitopatologi Indonesia Jurnal Hortikultura Indonesia (JHI) BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Agro Teknologi Tropika Indonesian Journal of Agricultural Science AGRIVITA, Journal of Agricultural Science Journal of Tropical Biodiversity and Biotechnology JURNAL BIOLOGI INDONESIA Jurnal Penelitian Pertanian Tanaman Pangan Journal of Tropical Crop Science Agritrop : Jurnal Ilmu-Ilmu Pertanian (Journal of Agricultural Science) Buletin Palma Berita Biologi

Sintho Wahyuning Ardie

Department Of Agronomy And Horticulture, Faculty Of Agriculture, IPB University, Dramaga Campus, Bogor 16680, Indonesia

Author-ID : 231376

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Economics, Econometrics & Finance Environmental Science Immunology & microbiology Medicine & Pharmacology Veterinary

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Peranan Fosfor dalam Meningkatkan Toleransi Tanaman Sorgum terhadap Cekaman Aluminium Lestari, Tri; ,, Trikoesoemaningtyas; Ardie, Sintho Wahyuning; Sopandie, dan Didy
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 45 No. 1 (2017): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

The management of P nutrition can be a good solution for aluminum toxicity and P nutrient deficiency in acid soil. This study aimed to determine the role of phosphorus in improving the tolerance of sorghum to Al stress in nutrient culture. This research was conducted at green house of IPB Bogor, tissue culture laboratory in the Department of AGH IPB and Balai Besar Pasca Panen Cimanggu Bogor, from January to November 2014. A completely randomized factorial design was used in three experiments. The results revealed that addition of P improved the tolerance of two sorghum genotypes to Al stress as indicated by the reduction in root length inhibition by Al, where P was more effective in tolerant genotype Numbu. Addition of P reduced the accumulation of Al in the root tissues as shown by the lighter intensity of hematoxylin staining, especially in Numbu. Aluminum stress increased the secretion of oxalate acid in both sorghum genotypes, where P lowered oxalate acid secretion in both genotypes. These facts showed that the role of P in improving the tolerance of sorghum to Al stress might be associated with the inhibition of Al absorption into the root tissues, although the mechanism is not yet known.Keywords: Al stress, organic acid secretion, role of P, root staining method, sorghum

Evaluasi Hasil dan Kandungan Pati Mutan Ubi Kayu Hasil Iradiasi Sinar Gamma Generasi M1V4 Subekti, Isnani; Khumaida, Nurul; Ardie, Sintho Wahyuning; Syukur, dan Muhamad
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 46 No. 1 (2018): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Cassava is one of the important starch producer crops. Although Indonesia ranks as the third largest cassava producer country, Indonesia still imports cassava starch to meet the domestic demand in food and non-food industries. Gamma ray irradiation has been implemented to generate cassava mutants in order to support development of superior cassava variety, especially high yield and high starch content, especially high yield and high starch content. The objectives of this research were to evaluate the yield potential and the starch content of gamma irradiated 40 putative cassava mutants at M1V4 generation. This research was arranged in randomized complete block design with three replications and conducted at Cikabayan Experimental Field, IPB, Bogor, from May 2016 to March 2017. The starch content was measured using gravimetric methods. The results showed that five mutants, G2142, G3151, G2141, G1143, and G3111, had higher tuber weight per plant (6-7 kg per plant) compared to the background ‘Gajah’ genotype (4.7 kg per plant). ‘G2112’ mutant genotype showed higher starch content (23.86%) compared to the background ‘Gajah’ genotype (17.65%). The specific gravity (SG) was positively correlated with starch content (r = 0.905), indicated that the higher the SG, the higher the starch content of cassava.Keywords: gravimetric, mutant, specific gravity (SG), yield

Heritabilitas, Karakterisasi, dan Analisis Clustergram Galur-galur Padi Dihaploid Hasil Kultur Antera Anshori, Muhammad Fuad; Purwoko, Bambang Sapta; Dewi, Iswari Saraswati; Ardie, Sintho Wahyuning; Suwarno, Willy Bayuardi; Safitri, dan Heni
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 46 No. 2 (2018): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Doubled haploid rice formation through anther culture becomes one of the alternatives in the plant breeding. The lines need to be characterized, clustered, and selected. This study aimed at determining heritability, characters, and cluster of doubled haploid lines obtained from anther culture. This experiment was conducted at IPB Rice Field Experiment Station, Darmaga, Bogor from October 2016 until January 2017. The experiment was managed according to randomized complete block design (RCBD) with 3 replications and genotype as the single factor treatment. Genotypes used were 60 genotypes consisting of 56 doubled haploid lines and 4 control varieties. The controls were Ciherang, Inpara 5, Inpari 29, and Inpari 34 Salin Agritan. The results showed that all characters have significant effect and high heritability (above 60%), so they could be used as a selection character. The tested genotypes could be grouped into three main groups. The first group consisted of 11 genotypes, the second group consisted of 44 genotypes, and the third group consisted of 5 genotypes. The main group determinant characters were productive tillers, percentage of filled grain number, plant height, panicle length, and weight of 1,000 grains. The number of filled grain and productive tillers can be used as selection characters together with yield potential.

Pertumbuhan dan Perkembangan Ubi Kayu Genotipe Lokal Manggu pada Panjang Setek Batang yang Berbeda Nurul Khumaida; Siswati, Leni; Ardie, Sintho Wahyuning
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 47 No. 3 (2019): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Cassava plant is widely cultivated by Indonesian people. Research on the development of roots and tubers of cassava is still limited. The purpose of this study was to evaluate the effect of stem cut size on the root development system and tuber filling in the cassava local genotype “Manggu”. This study consisted of two experiments namely experiments in polybag and in the field. The study was conducted in the IPB experimental field at Cikabayan in December 2017-July 2018. The experiment used a randomized completely block design (RCBD) with two treatments and three replications, namely cuttings of 15 cm and 30 cm. Observation of basal roots, number of nodal roots, length of basal roots, length of nodal roots, number of tubers, number of leaves, plant diameter, and plant height were carried out destructively every week. The results showed that the root bulking process occurred in the nodal root and basal roots and it was observed since 5 WAP. The results showed a positive correlation between the total number of tubers and the number of tubers formed from nodal and basal roots, the number of basal roots, and the length of basal roots and it was negatively correlated with the number of leaves. The number of tubers from nodal roots is positively correlated with the number of basal root tubers while it is negatively correlated with the length of the nodal root. The development of roots into cassava tubers occurred since 5 WAP. Cutting size treatments significantly did not affect root development. Keywords: basal root, nodal root, stem cutting, stem length

Morphological traits and Zn content of several cassava genotypes in nutrient solution culture Pratama, Syilvia Nosya; Sudarsono; Ardie, Sintho Wahyuning; Sukma, Dewi
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 51 No. 2 (2023): Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24831/ija.v51i2.44535

Leaf morphological characters and nutrient content in leaf tissue can vary depending on plant genotype. The nutrient solution culture can produce leaves with good nutritional content. Therefore, this research aimed to evaluate and validate cassava putative mutants' morphological traits and Zn content using a nutrient solution culture system. This research was carried out from January to May 2022 at the greenhouse of Cikabayan Experimental Garden, IPB University, Bogor. This study used two cassava cultivars and ten mutant genotypes from the cassava research team of IPB University, resulting from mutation breeding by gamma irradiation. The plant characters observed in this study included the number of leaves, length and width of the leaflet, leaf colour, plant height, fresh and dry weight leaf, and analysis of Zn content. The data were analyzed using ANOVA at a significant level of α = 5%, followed by Tukey's further test. The results showed that the G2D1-422 genotype had a higher Zn content than the wild type (Ratim/G2) and the other genotypes. The high Zn genotypes (G2D1-422) and low Zn genotypes (Ratim (G2) had not significantly different in fresh and dry leaf weight. Meanwhile, the plant genotype did not affect other growth characteristics such as plant height, the number of leaves, and the length and width of the leaflets, and overall, the characters were similar between the observed genotypes.

Mutation site identification confers glyphosate resistance in goosegrass (Eleusine indica L. Gaertn) from Jonggol, Bogor, Indonesia Ferdinans, Ferdinans; Guntoro, Dwi; Ardie, Sintho Wahyuning
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 51 No. 1 (2023): Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Glyphosate resistance is a serious problem in weed control, especially in oil palm plantations. However, evaluation of suspected resistant weeds such as Eleusine indica L. Gaertn at the gene level is still scarce in Indonesia. Here, target-site resistance to glyphosate of the EPSPS gene was evaluated. The objective of this study was to identify glyphosate resistance caused by mutation points in goosegrass biotypes from oil palm plantation in Jonggol, West Java, Indonesia. Bio-assay analysis was carried out by planting goosegrass in pots. Glyphosate was applicated using eight-level, namely 0, 0.125x, 0.25x, 0.5x, x, 2x, 4x, and 8x, where x was the recommended dose (972 g ha-1) for three replications. Weed damage was evaluated from plant biomass and then regression analysis was performed to obtain the LD50 and resistance index. The E. indica from block V had a reduced sensitivity status with 2.343 value of resistance index, and those from Block II biotype had a sensitive status with 1.588 resistance index. Alignment of the EPSPS gene showed no mutation was observed at two target-points, indicating that increasing resistance of E. indica in the study site could be controlled by other factors. Nevertheless, E. indica of block V biotype had a resistance potential due to the highest LD50. It is necessary to evaluate further the possibility of mutation in other target-points of the EPSPS gene. Keywords: bio-assay; EPSPS; LD50; resistance index; sequencing; target-site resistance

Confirmations on gene introgression events and hybridity for BLB resistance and yield in rice Ashan, Muh Aswad; Reflinur; Ardie, Sintho Wahyuning
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 51 No. 3 (2023): Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy)
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24831/jai.v51i3.51194

A superior variety of rice with high yield and resistance to diseases is expected to meet the increasing demand for this one of the most important carbohydrate sources worldwide. Bacterial leaf blight (BLB) caused by Xanthomonas oryzae pv. oryzae (Xoo) is one of the major biotic stresses limiting rice production. Combining BLB resistance and high-yield traits is possible through gene pyramiding. Introgression of targeted traits in the parental genotypes and hybridity confirmation in the F1 generation are important initial steps in gene pyramiding. This study aimed to confirm the presence of targeted genes in parent genotypes using specific molecular markers and to confirm the hybridity of F1 plants generated from three crosses combinations of Inpari 32 (as the female parent) and three Code-qTSN4 lines (as the male parents) using SSR markers. This study successfully confirmed the introgression of BLB-resistance genes in Inpari 32 (Xa4, Xa7, and Xa21) and in Code-qTSN4 (Xa4, Xa7). The introgression of yield-related QTL, qTSN4, was confirmed in the three Code-qTSN4 lines (A10-1, B12-2, and A16-5) by RM17483 marker. Five SSR markers (RM5, RM55, RM105, RM223, and RM561) successfully confirmed the hybridity of F1 derived from Inpari 32xA10-1 (five individuals), Inpari 32xB12-2 (one individual), Inpari 32xA16-5 (two individuals). These confirmed hybrids can be further evaluated in the F2 generation. Keywords: Gene pyramiding; molecular marker; SSR; qTSN4; Xoo.

ANALISIS IN SILICO PROTEIN BIOSINTESIS LIGNIN PADA TANAMAN SORGUM (Sorghum bicolor L. Moench) Windiastri, Vincentia Esti; Ardie, Sintho Wahyuning; Nugroho, Satya
Berita Biologi Vol 22 No 2 (2023): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/beritabiologi.2023.690

Lignin adalah bahan alam yang dapat dimanfaatkan sebagai bahan mentah dalam industri biofuel dan bioenergi. Peningkatan produksi lignin mendorong tersedianya sumber lignin terbaharukan dan ramah lingkungan. Sorgum berpotensi digunakan sebagai sumber biomassa untuk produksi lignin. Produksi lignoselulosa dari sorgum per tahunnya mencapai 19–47 ton/ha. Meskipun demikian, untuk memanfaatan sorgum sebagai sumber lignin secara optimal diperlukan peningkatan kandungan total lignin pada tanaman sorgum. Rekayasa genetika dalam modifikasi kandungan lignin dapat menjadi jawaban. Namun, informasi mengenai gen biosintesis lignin masih terbatas. Untuk memperoleh informasi protein biosintesis lignin pada sorgum (Sorghum bicolor L.Moench) dan protein homolognya dari tanaman monokotil yang lain, analisis bioinformatika mengenai protein biosintesis lignin telah dilakukan. Analisis dilakukan menggunakan program Blast-p, Mega-X, Conserved Domain Database, InterPro dan MEME. Hasil dari analisis yang dilakukan menggambarkan bahwa protein biosintesis lignin membentuk klaster searah dengan arah divergensi taksonomi tanaman monokotil. Protein lignin dari sorgum mengelompok bersama Mischanthus, tebu dan jagung. Pohon kekerabatan protein COMT menghasilkan bootstrap tertinggi untuk protein lignin sorgum di antara pohon filogeni protein-protein yang lain. Hasil penyejajaran keluarga protein COMT sorgum (XP_002445083.1) dengan protein COMT dari Mischanthus, tebu, jagung dan Arabidopsis menunjukkan bahwa protein XP_002445083.1 mempunyai karakter fungsional yang saling mirip. Sehingga dapat disimpulkan bahwa XP_002445083.1 diduga adalah protein COMT dari sorgum.

ANALISIS EKSPRESI RELATIF DAN PENYEJAJARAN GEN Bmr12 PADA AKSESI SORGUM (Sorghum bicolor L. Moench) Windiastri, Vincentia Esti; Sintho Wahyuning Ardie; Miyamoto, Takuji; Umezawa, Toshiaki; Satya Nugroho
Berita Biologi Vol 23 No 3 (2024): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/berita_biologi.2024.952

Gen Bmr12 (SbCOMT) diperkirakan mempunyai fungsi dalam penentuan kandungan total lignin pada sorgum karena mutasi gen Bmr12 menyebabkan kandungan total lignin tanaman mutan tereduksi. Pada penelitian sebelumnya telah dikelompokkan aksesi-aksesi sorgum di Indonesia berdasarkan kandungan lignin tinggi dan lignin rendah (Buleleng Empok, JP dan Pahat). Untuk mengetahui pola ekspresi gen Bmr12 pada kedua kelompok sorgum yang berbeda, maka dilakukan analisis level ekspresi relatif dari sorgum berlignin tinggi yang diwakili oleh aksesi KS, 4183, dan SMM; dan sorgum berlignin rendah yang diwakili oleh aksesi Buleleng Empok, JP dan Pahat. Sebagai data pendukung, dilakukan pula perunutan gen Bmr12 yang diisolasi dari aksesi KS, 4183, Buleleng Empok dan Pahat. Hasil analisis tersebut memperlihatkan adanya perbedaan pola ekspresi gen Bmr12. Level tertinggi ada pada aksesi 4183A, dan yang terendah adalah aksesi Pahat. Keempat aksesi lainnya mempunyai pola ekspresi yang hamper sama. Perunutan dan penyejajaran gen Bmr12 memperlihatkan adanya substitusi basa nitrogen yang mengarah ke substitusi asam amino pada aksesi KS. Namun substitusi tersebut tidak mengganggu fungsi gen Bmr12 dalam biosintesis lignin.

RAPD ANALYSIS TO DETECT SOMACLONAL VARIATION OF PINEAPPLE IN VITRO CULTURES DURING MICROPROPAGATION Roostika, Ika; Khumaida, Nurul; Ardie, Sintho Wahyuning
BIOTROPIA Vol. 22 No. 2 (2015): BIOTROPIA Vol. 22 No. 2 December 2015
Publisher : SEAMEO BIOTROP

Plant off-type formation has become a concern in pineapple micropropagation, and reliable methods are required to detect and minimize this problem. This study was conducted to confirm the occurrence of somaclonal variation during the micropropagation of pineapple clone Simadu. The effects of culture period (long vs. short duration) and regeneration methods (direct organogenesis, indirect organogenesis, and somatic embryogenesis) were evaluated to determine their contribution to somaclonal variation. RAPD analysis using ten primers was performed to confirm genetic variation. The results showed that RAPD assays could be applied for early detection of somaclonal variation in pineapple, with OPA primers performing better than OPJ primers. Phenotypic variation observed in four-year-old field-grown pineapple plants and plantlets was associated with genetic variation. The findings demonstrated that the long culture period was the main factor contributing to somaclonal variation, while regeneration method and plant growth regulators also played a role in inducing genetic changes. Newly initiated cultures showed a higher level of genetic similarity. Therefore, applying an appropriate micropropagation strategy is necessary to minimize plant off-types. It is recommended to avoid using long-term cultures as mother stock and to apply direct organogenesis rather than indirect organogenesis or somatic embryogenesis for pineapple micropropagation.

Co-Authors , Krisantini Abdul Qadir Agus Zainudin Agustina, Ferra Anggita Ahmad Fathoni Aji, Titistyas Gusti Alex Hartana Anas D Susila Anas Dinurrohman Susila Anjarsari, Nurul Aeni Anshori, Muhammad Fuad Ashan, Muh Aswad Azis Natawijaya Bambang Sapta Purwoko Butarbutar, Lidya Kristina Sari Cahyono, Andy Agus Chusnah, Uswatun Dewi Sukma Didy Sopandie Dwi Guntoro Enny Sudarmonowati Enung Sri Mulyaningsih fatimah Fatimah Ferdinans, Ferdinans Ghulammahdi, Munif Hagia Sophia Khairani HAJRIAL ASWIDINNOOR Hariyadi Hartana, Alex Heni Safitri Hermanasari, Rini Ika Roostika Indrastuti A. Rumanti Irmanida Batubara Ismail maskromo Iswari Saraswati Dewi Iswari Saraswati Dewi Kansa Dianti Putri Karjunita, Nike Lainufar, Putri Aulia Lefin Kafindra Luthfiani, Marisa Vidya M. Syukur Mahrup Mahrup, Mahrup Mashuri Waite maskromo, Ismail Miyamoto, Takuji Mohamad Rahmad Suhartanto Muaz Adbdul Karim Muaz Adbdul Karim Muhamad Syukur Munif Ghulamahdi N. Sri Hartati, N. Sri N. Sri i Hartati Nampiah Sukarno Natawijaya, Azis Nugraha, Yudhistira Nugroho, Rizki Bagus Nurul Fauziah Nurul Khumaida Nurul Khumaida Nurulalia, Lia Pahlevi, M Reza Parlindo, Fitra Pasaribu, Pesta Maria Hotnauli Pratama, Syilvia Nosya Prita Sari Dewi Rahmawati, Rika Sri Rahmi Henda Yani Ramadaniarto Rizqullah Reflinur Resti Putri Septyani Resti Putri Septyani Rika Miftakhul Jannah Rizqullah, Ramadaniarto Romy Agus Saputro Romy Agus Saputro Rubiyo Rubiyo Rumanti, Indrastuti A. Sadewi Maharani, Sadewi Saenal, Nirwanti Safitri, dan Heni Safitri, Vika Ayu Satya Nugroho Satya Nugroho Siswati, Leni Siti Kurniawati Slamet Susanto Soekisman Tjitrosoedirdjo SRI RAHAYU Sri Ratnawati Subekti, Isnani Sudarsono Sudarsono Sudarsono Sudarsono Sudarsono, Sudarsono Suwarno ,, Suwarno Suwarno Suwarno Suwarno, Suwarno Suwarno, Suwarno Syawaluddin, Dwi Dana Syukur, M. TRI JOKO SANTOSO TRI KOESOEMANINGTYAS Tri Lestari Trias Sitaresmi Trikoesoemaningtyas Tsugama, Daisuke Ulfa, Umi Tahniah Umezawa, Toshiaki Vincentia Esti Windiastri, Vincentia Esti Widowati, Sartika Willy Bayuardi Suwarno Yudhistira Nugraha Yudiansyah Yudiansyah Yudiwanti Wahyu E. Kusumo Yuli Irvana Dewi Yuli Sulistyowati Yullianida , Za’aziza Ridha Julia

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