Sintho Wahyuning Ardie
Department Of Agronomy And Horticulture, Faculty Of Agriculture, IPB University, Dramaga Campus, Bogor 16680, Indonesia

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Pematahan Dormansi Rimpang Kaempferia parvifloraWall. ExBaker Muaz Adbdul Karim; Sintho Wahyuning Ardie; Nurul Khumaida
Buletin Agrohorti Vol. 2 No. 1 (2014): Januari 2014
Publisher : Departemen Agronomi dan Hortikultura

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (445.955 KB) | DOI: 10.29244/agrob.2.1.104-114

Abstract

Kaempferiaparviflora (Zingiberaceae family) has a potency to be developed for a new medicinal plants in Indonesia. One of problems in its cultivation is the lack of planting material availability due to the long dormant period. The objectives of this research were to study the effect of various concentrations of BAP and ethephon, and also the effect of the prior growing condition to the dormancy breaking and shoot growth of K.parviflora rhizome. Two separate experiments were applied for two lots of rhizomes namely Cikabayan and Pasir Sarongge lots. Each experiment was arranged in completely random design with 2 factors. Experiment-1 used 4 concentrations of BAP (0, 50, 100, and 150 ppm) as the first factor. While, experiment-2 used 5 concentrations of ethephon (0, 200, 400, 600 and 800 ppm) as the first factor. The second factor in both experiments were prior shade condition at 55% artificial shading and natural shading. A rhizome without soaked application (control) and a rhizome with soaked by aquadestilata without PGRs (0 ppm) were used as compared items. Data was analyzed with ANOVA. Combined Analysis of Variance and t-test were performed in order to compare between 2 lots. Result showed that concentration of BAP until 150 ppm and concentration of ethephon until 800 ppm were not effective to stimulate bud induction time. Meanwhile, rhizome from 55% artificial shading had a shorter periode of dormancy than the natural shading. In the addition, plants from Pasir Sarongge rhizomes lot had more plant number, plant height, leaf number, leaf length, and leaf width than Cikabayan rhizomes lot.
Budidaya Tanaman Akar Wangi (Vetiveria zizanioides (L.) Nash) dalam Wadah: Pengaruh Jenis Media Tanam dan Jumlah Bibit Resti Putri Septyani; Sintho Wahyuning Ardie; Slamet Susanto
Buletin Agrohorti Vol. 1 No. 4 (2013): Oktober 2013
Publisher : Departemen Agronomi dan Hortikultura

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (537.441 KB) | DOI: 10.29244/agrob.1.4.111-121

Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh komposisi media tanam dan jumlah bibit dalam budidaya tanaman akar wangi (Vetiveria zizanioides) secara hidroponik menggunakan polybag terhadap pertumbuhan tanaman akar wangi varietas Verina 2. Penelitian dilakukan di rumah kaca Kebun Percobaan Cikabayan Bawah, IPB dengan elevasi 240 m di atas permukaan laut (dpl) mulai dari bulan November 2012 hingga Juli 2013. Penelitian disusun berdasarkan rancangan kelompok lengkap teracak (RKLT) dengan dua faktor dan lima ulangan. Faktor pertama adalah komposisi media tanam (v/v) yang terdiri atas tiga taraf, yaitu 100% arang sekam, arang sekam : styrofoam (2:1), dan arang sekam : styrofoam (1:1). Faktor kedua adalah jumlah bibit dalam satu polybag yang terdiri atas dua taraf yaitu satu bibit dan dua bibit. Hasil penelitian menunjukkan bahwa interaksi antara komposisi media tanam dan jumlah bibit per polybag tidak berpengaruh nyata terhadap pertumbuhan tanaman dan pertumbuhan akar tanaman akar wangi. Tanaman yang ditanam pada media arang sekam : styrofoam (1:1) memiliki jumlah daun, jumlah anakan, jumlah akar, panjang akar, kandungan klorofil, dan karotenoid yang lebih tinggi dibandingkan tanaman yang ditanam pada media lainnya. Penanaman dua bibit per polybag menghasilkan jumlah daun, jumlah anakan, bobot basah dan kering tajuk, dan jumlah akar yang lebih tinggi dibandingkan dengan satu bibit per polybag. Hasil tersebut menunjukkan bahwa penanaman dua bibit per polybag pada media                    arang sekam : styrofoam (1:1) menghasilkan pertumbuhan vegetatif dan pertumbuhan akar terbaik pada tanaman akar wangi.
Induksi Rimpang Mikro Kaempferia parviflora secara In Vitro dengan Penambahan BAP dan Sukrosa Lefin Kafindra; Nurul Khumaida; Sintho Wahyuning Ardie
Jurnal Hortikultura Indonesia Vol. 6 No. 1 (2015): Jurnal Hortikultura Indonesia
Publisher : Indonesian Society for Horticulture / Department of Agronomy and Horticulture

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (791.736 KB) | DOI: 10.29244/jhi.6.1.54-63

Abstract

ABSTRACTKaempferia  parviflora  is a medicinal plant  that contains  secondary metabolites  and  effective in alleviating some  diseases.  Rapid multiplication of K.  parviflora  through  its rhizome is hampered by the long period needed to produce the rhizome. The objectives of this research were to obtain the best sucrose  concentration  and  to  determine  the  best  BAP  concentration  in the in vitro  microrhizome induction of  K.  parviflora.  The experiment was arranged in a randomized complete block design with two factors and three replications.  The first factor was BAP concentration, consisted of three levels i.e. 0, 2, and 4 mg L1. The second factor was sucrose concentration, consisted of four levels  i.e.  0,  30,  60,  and  90  g  L1.  Data  obtained  were  analyzed by  F-test,  followed  by  DMRT (Duncan  Multiple Range Test) at 5% significant level.  Sucrose  concentration  significantly affected the  number of shoots  and plantlets  fresh  weight.  The results showed that  K.  parviflora  could form micro  rhizome  in  vitro,  as  indicated  by  similar  anatomical  structure compared  to  the  rhizome produced in vivo. K. parviflora cultured in MS medium containing 90 g L1sucrose without BAP had the highest number of micro rhizome at 8 weeks after culture.Keywords: health, black galingale, traditional medicine ABSTRAKKaempferia  parviflora  merupakan  tanaman  obat  yang  mengandung metabolit  sekunder  dan berkhasiat  untuk  mengobati  berbagai  macam penyakit.  Perbanyakan  K.  parviflora  menggunakan rimpang terkendala oleh lamanya waktu untuk memproduksi rimpang di lapang, yaitu mencapai 8 bulan setelah  tanam.  Tujuan  penelitian  ini  ialah  memperoleh  konsentrasi sukrosa terbaik  dan konsentrasi  BAP  terbaik  dalam  induksi  rimpang  mikro  K. parviflora.  Percobaan  ini  disusun berdasarkan  rancangan  kelompok  lengkap teracak  dengan  dua  faktor  dan  tiga  ulangan.  Faktor pertama  adalah konsentrasi  BAP  dengan  3  taraf,  yaitu  0,  2,  dan  4  mg  L1.  Faktor kedua adalah konsentrasi  sukrosa  dengan  4  taraf,  yaitu  0,  30,  60,  dan  90  g L1.  Data  yang  diperoleh  dianalisis menggunakan  uji  F  dan  apabila berpengaruh  nyata,  maka  dilanjutkan  dengan  uji  lanjut  DMRT (Duncan Multiple Range Test) pada taraf α= 5 %. Hasil penelitian menunjukkan bahwa K. parviflora dapat membentuk rimpang mikro  in vitro. Hal tersebut ditunjukkan oleh struktur anatomi rimpang mikro  yang  serupa  dengan rimpang  in  vivo.  K. parviflora  yang  dikulturkan  pada  media  MS yang mengandung 90 g L1sukrosa tanpa BAP memiliki jumlah rimpang mikro tertinggi pada 8 MSP.Kata kunci: kesehatan, kencur hitam, obat tradisional
SALINITY TOLERANCE OF SEVERAL RICE GENOTYPES AT SEEDLING STAGE Heni Safitri; Bambang Sapta Purwoko; Iswari Saraswati Dewi; Sintho Wahyuning Ardie
Indonesian Journal of Agricultural Science Vol 18, No 2 (2017): December 2017
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v18n2.2017.p63-68

Abstract

Salinity is one of the most serious problems in rice cultivation. Salinity drastically reduced plant growth and yield, especially at seedling stage. Several rice genotypes have been produced, but their tolerance to salinity has not yet been evaluated. The study aimed to evaluate salinity tolerance of rice genotypes at seedling stage. The glasshouse experiment was conducted at Cimanggu Experimental Station, Bogor, from April to May 2013. Thirteen rice genotypes and two check varieties, namely Pokkali (salt tolerant) and IR29 (salt sensitive) were tested at seedling stage. The experiment was arranged in a randomized complete block design with three replications and two factors, namely the levels of NaCl (0 and 120 mM) and 13 genotypes of rice. Rice seedlings were grown in the nutrient culture (hydroponic) supplemented with NaCl at different levels. The growth and salinity injury levels of the genotypes were recorded periodically. The results showed that salinity level of 120 mM NaCl reduced seedling growth of all rice genotypes, but the tolerant ones were survived after 14 days or until the sensitive check variety died. Based on the visual injury symptoms on the leaves, five genotypes, i.e. Dendang, Inpara 5, Inpari 29, IR77674-3B-8-2-2-14-4-AJY2, and IR81493-BBB-6-B- 2-1-2 were tolerant to 120 mM salinity level, while Inpara 4 was comparable to salt sensitive IR29. Hence, Inpara 4 could be used as a salinity sensitive genotype for future research of testing tolerant variety. Further evaluation is needed to confirm their salinity tolerance under field conditions. 
IMPLICATION OF GENE ACTION AND HERITABILITY UNDER STRESS AND CONTROL CONDITIONS FOR SELECTION IRON TOXICITY TOLERANT IN RICE Yudhistira Nugraha; Sintho Wahyuning Ardie; Munif Ghulamahdi; Suwarno Suwarno; Hajrial Aswidinnoor
AGRIVITA, Journal of Agricultural Science Vol 38, No 3 (2016): OCTOBER
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v38i3.740

Abstract

Iron toxicity is major constraint of rice production in irrigated-lowland of tropical regions. Improvement the tolerance of the rice cultivar to iron toxicity needs the information some genetics parameters of the selected characters. Here we study the estimation of gene action and heritability of the grain yield and its component under iron-toxic stress and control field conditions in rice. The iron-toxic tolerant rice cultivars, Pokkali and Mahsuri were crossed with the sensitive cultivar, Inpara5 to develop six generation populations. The breeding materials were grown in the iron toxicity site and control in Taman Bogo, Lampung Indonesia. The sensitive parent and BC1P1 had lower stress tolerance index (STI) compared to the tolerant parent F1, F2 and BC1P2.  Most of the characters including the grain yield were fitted the best model in five parameters which were more prominent with interactive epistasis of duplicate and complementary gene action.  The heritability’s under control were more higher compared to iron toxicity stress condition. Delaying selection to later generations and combining with the shuttle breeding between stressed and controlled environments were the best strategy for improving the grain yield and tolerance to iron toxicity in rice.
Parameters and Secondary Characters for Selection of Tolerance Rice Varieties under Stagnant Flooding Condition Trias Sitaresmi; Willy B. Suwarno; Indrastuti A. Rumanti; Sintho W. Ardie; Hajrial Aswidinnoor
AGRIVITA, Journal of Agricultural Science Vol 41, No 2 (2019)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v41i2.957

Abstract

Determination of secondary characters during stagnant flooding (SF) is considered important for breeders as the selection criteria in developing SF rice tolerant varieties. Aims of this study were to find agronomical variation and to determine secondary traits that were related to SF tolerance among the rice varieties. Experiment was conducted at Indonesian Center for Rice Research in 2015. Ten rice genotypes were grown in RCBD with three replications under normal and gradual flooding conditions. Agronomic data were gathered and analysed using combined ANOVA, correlation, multiple linear regressions and genetic variability. Results showed that 92.3% variance of stress tolerance index (STI) were presented from a linear model involving weight of 100-grains, panicle length, stem diameter, intensity of leaf green color and stem length. Stem length, intensity of leaf green color, and panicle length had broad genetic variability and high heritability these characters were important criteria when selecting the traits under flowing stress. Number of productive tillers was correlated with grain yield under SF and highly heritable, thus considered as one of determining characters for stagnant flooding tolerance. Based on STIStd, Ciherang and INPARI 30 showed more adaptive performance, while IR 42 had the least when grown under 50-60 cm stagnant water depth.
Analysis of Variance, Heritability, Correlation and Selection Character of M1 V3 Generation Cassava (Manihot esculenta Crantz) Mutants Rahmi Henda Yani; Nurul Khumaida; Sintho Wahyuning Ardie; Muhamad Syukur
AGRIVITA, Journal of Agricultural Science Vol 40, No 1 (2018): FEBRUARY
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v40i1.844

Abstract

Information about genetic variability and correlation between qualitative character and yield are important to support a selection program. The objective of this research was to determine genetic variability, heritability, and path analysis of M1 V3 cassava mutants’ characters. This research was conducted at Bogor Agricultural University Experimental Field Research from May 2014 to May 2015. This research used 32 mutants from five cassava parent lines which were Malang-4 and Adira-4 (national varieties), UJ-5 (Introduction variety from Thailand), and two local genotypes from Halmahera which were Jame-jame and Ratim. The results showed that gamma ray irradiation increased variability from five cassava genotypes. Characters that had high heritability were length of leaf lobe, lengthof petiole, stem diameter, and the height of plant. The path correlation analysis showed that number of tubers, number of economic tuber (> 20 cm), height to first branchingand stem diameter had direct correlation with tuber mass per plant. The characters can be used for the selection of M1 V4 generation.
Different Root Anatomical Changes in Salt-tolerant and Salt-sensitive Foxtail Millet Genotypes Nike Karjunita; Nurul Khumaida; Sintho Wahyuning Ardie
AGRIVITA, Journal of Agricultural Science Vol 41, No 1 (2019)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v41i1.1786

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Foxtail millet is relatively tolerant to salinity stress and thus can be grown in salinity affected areas. This study was conducted to identify anatomical changes in the roots of foxtail millet genotypes with different tolerance level to salt stress. Four foxtail millet genotypes, namely ICERI-5 and ICERI-6 (salt tolerant) and ICERI-4 and ICERI-10 (salt sensitive), were grown hydroponically for 1 week prior to 60 and 120 mM salt stress treatments. Root anatomical changes were observed on the fifth day after treatments. The results showed that salt stress significantly induced some anatomical changes in the roots of foxtail millet, i.e. increased epidermis and cortex thickness, increased root diameter, and increased the number of root hairs. The increase in epidermis thickness, root diameter and the number of root hairs due to the salt application were more pronounced in the sensitive genotypes. Number of protoxylem in the tolerant genotypes were significantly increased due to salt stress, however salinity significantly decreased the number of protoxylem in the sensitive genotypes. The different anatomical changes under salt stress between the tolerant- and sensitive genotypes indicated that some anatomical attributes of the roots might determine the salt tolerance level of foxtail millet.
Keragaan Dan Keragaman Karakter Agronomi Pada Populasi Intra Dan Inter Famili Dura Elit Koleksi Taman Buah Mekarsari [Variability of Some Agronomic Traits Within and Between Family of Mekarsari Elite Dura Population] Azis Natawijaya; Sintho WA; Ismail maskromo; M. Syukur; Alex Hartana; Sudarsono Sudarsono
Buletin Palma Vol 18, No 1 (2017): Juni, 2017
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/bp.v18n1.2017.23-32

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Genetic variability is a basis for plant genetic improvement. Evaluation of genetic variability in oil palm populations helps breeders in determining traits for selection, determining appropriate selection methods, and identifying promising families. The study was aimed to evaluate the genetic variability within and between family in elite dura population. The research was conducted from January 2014 until December 2016 at Mekarsari Research Station. A total of 287 individuals from 18 different genetic backgrounds were used as research materials. The results showed that the phenotypic variability within and between family are wide. No single family or genotype has any superior characters yet. So a breeding approach is needed to converge all good traits into one genotype or population.ABSTRAKVariabiltas genetik merupakan dasar untuk perbaikan genetik tanaman. Evaluasi variabilitas genetik pada populasi kelapa sawit membantu pemulia dalam menentukan karakter dan kriteria seleksi, menentukan metode seleksi yang tepat, dan mengidentifikasi famili-famili potensial yang memiliki karakter harapan. Penelitian ini ditujukan untuk mengevaluasi keragaman beberapa karakter agronomi pada populasi kelapa sawit dura elit. Penelitian dilakukan pada bulan Januari 2014 sampai dengan Desember 2016 di Kebun Percobaan PT. Sasaran Ehsan Mekarsari. Total 287 individu yang berasal dari 18 latar belakang genetik yang berbeda digunakan sebagai bahan tanaman untuk penelitian. Hasil penelitian menunjukkan bahwa karakter agronomi pada populasi dura koleksi Mekarsari menunjukkan keragaman yang tinggi baik intra maupun inter famili. Karakter rasio mesocarp dan jumlah biji per tandan memiliki variasi fenotipe yang terluas dan famili atau genotipe yang memiliki semua karakter unggul belum ada. Oleh karena itu, diperlukan pendekatan pemuliaan untuk menghimpun sifat-sifat baik ke dalam satu populasi atau memfiksasi gen-gen yang tersebar di antara famili atau genotipe ke dalam satu populasi
ADAPTASI TANAMAN Hoya diversifolia BLUME PADA INTENSITAS CAHAYA TINGGI [ ADAPTATION OF Hoya diversifolia BLUME TO HIGH-LIGHT INTENSITY ] Sintho Wahyuning Ardie; Sri Rahayu; Anas D Susila; Didy Sopandie
AGRITROP Vol 12, No 1 (2014): Agritrop: Jurnal Ilmu-Ilmu Pertanian
Publisher : Universitas Muhammadiyah Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32528/agr.v12i1.697

Abstract

Hoya diversifolia Blume merupakan salah satu anggota famili Asclepiadaceae yang potensial dikembangkan sebagai tanaman hias, terutama sebagai dekorasi pergola.  Akan tetapi, tanaman ini hidup pada kondisi ternaung di habitat aslinya dan pertumbuhannya pada kondisi cahaya matahari penuh belum diketahui.  Oleh karena itu, tujuan dari penelitian ini adalah untuk mempelajari adaptasi tanaman H. diversifolia Bl. pada intensitas cahaya yang berbeda.  Penelitian disusun berdasarkan rancangan tersarang, dengan satu faktor dan tiga ulangan.  Ulangan tersarang di dalam intensitas cahaya yang terdiri atas tiga taraf, yaitu 28.2 (cahaya penuh), 20.8 (37% naungan) dan 10.1 Klux (64% naungan). Hasil penelitian menunjukkan bahwa tinggi tanaman dan jumlah daun tidak dipengaruhi oleh intensitas cahaya.  Tanaman yang ditanam pada kondisi cahaya penuh memiliki jumlah buku lebih sedikit, daun yang lebih tipis, dan warna daun yang kekuningan.  Hasil penelitian ini mengindikasikan bahwa H. diversifolia Bl. dapat ditanam pada kondisi naungan hingga ternaung sebagian, dan menanam pada kondisi cahaya matahari penuh tidak direkomendasikan. Kata kunci: Asclepiadaceae, intensitas cahaya, naungan, tanaman hias
Co-Authors , Krisantini Abdul Qadir Agus Zainudin Agustina, Ferra Anggita Ahmad Fathoni Aji, Titistyas Gusti Alex Hartana Anas D Susila Anas Dinurrohman Susila Anjarsari, Nurul Aeni Anshori, Muhammad Fuad Ashan, Muh Aswad Azis Natawijaya Bambang Sapta Purwoko Butarbutar, Lidya Kristina Sari Cahyono, Andy Agus Chusnah, Uswatun Dewi Sukma Didy Sopandie Dwi Guntoro Enny Sudarmonowati Enung Sri Mulyaningsih fatimah Fatimah Ferdinans, Ferdinans Ghulammahdi, Munif Hagia Sophia Khairani HAJRIAL ASWIDINNOOR Hariyadi Hartana, Alex Heni Safitri Hermanasari, Rini Ika Roostika Indrastuti A. Rumanti Irmanida Batubara Ismail maskromo Iswari Saraswati Dewi Iswari Saraswati Dewi Kansa Dianti Putri Karjunita, Nike Lainufar, Putri Aulia Lefin Kafindra Luthfiani, Marisa Vidya M. Syukur Mahrup Mahrup, Mahrup Mashuri Waite maskromo, Ismail Miyamoto, Takuji Mohamad Rahmad Suhartanto Muaz Adbdul Karim Muaz Adbdul Karim Muhamad Syukur Munif Ghulamahdi N. Sri Hartati, N. Sri N. Sri i Hartati Nampiah Sukarno Natawijaya, Azis Nugraha, Yudhistira Nugroho, Rizki Bagus Nurul Fauziah Nurul Khumaida Nurul Khumaida Nurulalia, Lia Pahlevi, M Reza Parlindo, Fitra Pasaribu, Pesta Maria Hotnauli Pratama, Syilvia Nosya Prita Sari Dewi Rahmawati, Rika Sri Rahmi Henda Yani Ramadaniarto Rizqullah Reflinur Resti Putri Septyani Resti Putri Septyani Rika Miftakhul Jannah Rizqullah, Ramadaniarto Romy Agus Saputro Romy Agus Saputro Rubiyo Rubiyo Rumanti, Indrastuti A. Sadewi Maharani, Sadewi Saenal, Nirwanti Safitri, dan Heni Safitri, Vika Ayu Satya Nugroho Satya Nugroho Siswati, Leni Siti Kurniawati Slamet Susanto Soekisman Tjitrosoedirdjo SRI RAHAYU Sri Ratnawati Subekti, Isnani Sudarsono Sudarsono Sudarsono Sudarsono Sudarsono, Sudarsono Suwarno ,, Suwarno Suwarno Suwarno Suwarno, Suwarno Suwarno, Suwarno Syawaluddin, Dwi Dana Syukur, M. TRI JOKO SANTOSO TRI KOESOEMANINGTYAS Tri Lestari Trias Sitaresmi Trikoesoemaningtyas Tsugama, Daisuke Ulfa, Umi Tahniah Umezawa, Toshiaki Vincentia Esti Windiastri, Vincentia Esti Widowati, Sartika Willy Bayuardi Suwarno Yudhistira Nugraha Yudiansyah Yudiansyah Yudiwanti Wahyu E. Kusumo Yuli Irvana Dewi Yuli Sulistyowati Yullianida , Za’aziza Ridha Julia