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All Journal HAYATI Journal of Biosciences EKSAKTA: Journal of Sciences and Data Analysis Indonesian Journal of Cancer INDONESIAN JOURNAL OF PHARMACY JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA The Indonesian Biomedical Journal Paediatrica Indonesiana Journal of Medicine and Health Molecular and Cellular Biomedical Sciences (MCBS) Syntax Literate: Jurnal Ilmiah Indonesia Farmasains : Jurnal Ilmiah Ilmu Kefarmasian Majalah Farmasi dan Farmakologi (Trends in Pharmacy and Pharmaceutical Sciences) JFL : Jurnal Farmasi Lampung Eksakta : Berkala Ilmiah Bidang MIPA Jurnal Ilmu Kefarmasian Indonesia Majalah Kedokteran Andalas Jurnal Jamu Indonesia Majalah Patologi Indonesia Jurnal Ekonomi Muhammadiyah Journal of Geriatric Indonesian Journal of Cancer Pharmaceutical Sciences and Research (PSR) Indonesian Journal of Medical Chemistry and Bioinformatics Jurnal Kefarmasian Indonesia Indonesian Journal of Jamu Journal of Medicine and Health
Wawaimuli Arozal
Universitas Indonesia
Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Neuroscience Public Health Social Sciences Other

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Potential Anti-Senescence Effect of Extract from Andrographis paniculata Herbal Plant and Its Bioactive Compounds: A Systematic Review Khatimah, Nurul Gusti; Arozal, Wawaimuli; Barinda, Agian Jeffilano; Antarianto, Radiana Dhewayani; Hardiany, Novi Silvia; Shimizu, Ippei; Fadhillah, Muhamad Rizqy
Molecular and Cellular Biomedical Sciences Vol 8, No 3 (2024)
Publisher : Cell and BioPharmaceutical Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21705/mcbs.v8i3.432

Abstract

The rapid aging of the global population is a major worldwide issue because of the close relationship between age and the development of several diseases. Aging or senescence is among the most widely studied topics at the moment. However, no pharmaceuticals have been developed that claim to possess anti-senescence properties. Andrographis paniculata, is a medicinal plant found widely throughout tropical and subtropical Asia. This review aims to identify the potential anti- senescence effect of A. paniculata extract and its bioactive compounds. By following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines, five databases were used and in vivo and in vitro studies were included in this review. A. paniculata extracts and their bioactive compounds exert anti-senescence properties through their anti-inflammatory and antioxidant properties. This herb and its compounds enhanced memory, cognitive function and behaviour in Alzheimer's disease. The extract also promoted cell cycle progression and proliferation in the skin. In addition, andrographolide exhibited anti-senescence effects in endothelial cells through the activation of PI3K/Akt/Nrf and PI3K/Akt/AP-1 pathways. A. paniculata along with its bioactive compounds including andrographolide and 14-deoxyandrographolide, may have the potential to be used as anti-senescence through anti-inflammatory and antioxidant properties. However, the specific markers to evaluate the senescence are necessary to be conducted. Any clinical trials should be done to establish these findings. Since in clinical settings this potential herbal may be used for long-life time, the safety profile and toxicity of A. paniculata should be considered. Keywords: herbal plants, Andrographis paniculata, andrographolide, bioactive compounds, senescence
Identification of Active Compounds of Leaf Extract and Seed Oil of Moringa oleifera in TrkB Receptor as Neuroprotective by Molecular Docking Muhamad Sadam Safutra; Fadilah; Wawaimuli Arozal; Agian Jeffilano Barinda
EKSAKTA: Berkala Ilmiah Bidang MIPA Vol. 25 No. 02 (2024): Eksakta : Berkala Ilmiah Bidang MIPA (E-ISSN : 2549-7464)
Publisher : Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Negeri Padang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/eksakta/vol25-iss02/442

Abstract

The functional ability of the brain will decline progressively during aging which usually involves changes in plasticity. BDNF is one of the neurotrophins that regulates plasticity via TrkB receptors. So the potential of Moringa oleifera leaf and seed oil extracts was identified as neuroprotective on the interaction of TrkB receptors with molecular docking. The active compounds of Moringa oleifera leaf extract and seed oil were obtained from literature studies. Drug-likeness and ADMETox analysis were carried out using the SwissAdme and the AdmetSAR webserver. The molecular docking was carried out using the Pyrx Vina application and visualization is done using the Discovery Studio Biovia application. The docking results showed the best compounds namely luteolin, stigmasterol, and moringin as a marker compound and showed the interaction of hydrogen and hydrophobic bonds at the active site of the prediction results. So it can be concluded that compounds in the leaves and seed oil of Moringa oleifera, namely luteolin, stigmasterol, and moringin are predicted as ingredients that can activate TrkB receptors in the aging process.
Identification of Potential Biomarkers for Hypertensive Nephropathy by Bioinformatics Analysis Fajarido, Ariski; Fadilah, Fadilah; Wawaimuli Arozal
EKSAKTA: Berkala Ilmiah Bidang MIPA Vol. 25 No. 04 (2024): Eksakta : Berkala Ilmiah Bidang MIPA (E-ISSN : 2549-7464)
Publisher : Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Negeri Padang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/eksakta/vol25-iss04/537

Abstract

Hypertensive nephropathy (HN) is a common complication of chronic hypertension that leads to kidney damage. This study aimed to identify potential biomarkers and key pathways associated with HN using bioinformatics tools. Gene data related to HN were retrieved from GeneCards and the Comparative Toxicogenomics Database (CTD), resulting in 89 genes from GeneCards and 10,898 genes from CTD. A Venn diagram revealed 58 overlapping genes, which were then analyzed using Protein-Protein Interaction (PPI) networks and the CytoHubba plugin in Cytoscape. The Maximal Clique Centrality (MCC) algorithm identified 10 hub genes, including ACE, AGT, ACE2, AGTR1, and AGTR2, integral to the renin- angiotensin-aldosterone system (RAAS). Functional enrichment analysis using Gene Ontology (GO) and KEGG pathways revealed that the most significant biological process was regulating systemic arterial blood pressure by the Renin-Angiotensin system, with the renin-angiotensin system pathway being the most highly enriched. Further visualization using ShinyGo highlighted the involvement of key genes in the RAAS pathway. These findings provide valuable insights into the molecular mechanisms underlying HN and suggest that bioinformatics approaches can aid in the identification of specific biomarkers for early diagnosis, non-invasive monitoring, and targeted treatments for HN in the future.
(RETRACTION) Efek Centella asiatica terhadap resistensi insulin pada parameter biokimia mencit yang diberi diet tinggi lemak Dwita, Nounik Cheri; Arozal, Wawaimuli; Barinda, Again Jeffilano; Safutra, Sadam
Majalah Kedokteran Andalas Vol 46, No 9 (2024): Supplementary Januari 2024
Publisher : Faculty of Medicine, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/mka.v46.i9.p1422-1431.2024

Abstract

Efek Centella asiatica terhadap resistensi insulin pada parameter biokimia mencit yang diberi diet tinggi lemak. 2024; 1422-1431: The above article, published online on January, 2024 in Majalah Kedokteran Andalas, https://doi.org/10.25077/mka.v46.i9.p1434-1443.2024 , has been retracted by agreement between the authors and the journal's Editor in Chief, dr. Hirowati Ali, Ph.D. The retraction has been agreed after the authors asked to retract the article due to a misattribution of authorship.
The Role of Centella asiatica and Its Main Bioactive Compound, Asiatic Acid in Cardiac Remodeling: A Systematic Review of Animal Studies Fadhillah, Muhamad Rizqy; Arozal, Wawaimuli; Wibowo, Heri; Bustami, Arleni; Primadhani, Suci Widya; Gusti Khatimah, Nurul; Putri, Rizky Clarinta; Riski Amanda, Clara; Azizah, Nur
Molecular and Cellular Biomedical Sciences Vol 9, No 1 (2025)
Publisher : Cell and BioPharmaceutical Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21705/mcbs.v9i1.482

Abstract

Cardiac remodeling is a phenotype of heart failure characterized by a molecular, cellular, and interstitial change in the heart, which manifests in the change of size and function of the heart after specific insults with multiple mechanisms. Centella asiatica (CA) and its main bioactive triterpenoid, asiatic acid (AA), pose antioxidant and anti-inflammatory effects. Still, no adequate clinical trials support the potency of CA and AA as anti-cardiac remodeling. Hence, this systematic review aims to provide an in-depth analysis of CA extract and AA in animal studies' prevention or therapy of cardiac remodeling. The search strategies were conducted based on preferred reporting Items for systematic reviews and meta-analysis (PRISMA) protocol through Pubmed, EMBASE, Scopus, and Web of Science using keywords as follows: “Centella asiatica” OR “Asiatic Acid” AND “Cardiac Remodeling” OR “Cardiac Hypertrophy” OR “Cardiac Fibrosis” along with their synonym. The data collected included hemodynamic parameters based on echocardiography, biomolecular tests such as quantitative polymerase chain reaction (qPCR), Western blotting, or biochemistry procedures. The paper quality was assessed using Systematic Review Centre for Laboratory Animal Experimentation (SYRCLE) risk of bias (RoB). The previous selected study has shown that CA and AA might prevent and cure cardiac remodeling by inhibiting various pathways and protein expressions through AMPKα, NOX2/4, PI3K/Akt/mTOR, p70S6K, YAP/TAZ, and IL-1β, IL-6, and IL-18 cytokines. CA and AA, thus, exhibit cardioprotective effects in the animal model, which need to be confirmed in the clinical trials on humans. Keywords: cardiac remodeling, cardiac hypertrophy, cardiac fibrosis, Centella asiatica, asiatic acid
Efek Andrographolide Oral Terhadap Biomarker Jantung pada Tikus yang Diinduksi Doxorubicin Silmi Hanifah; Wawaimuli Arozal; Vetnizah Juniantito
Journal of Medicine and Health Vol 5 No 2 (2023)
Publisher : Universitas Kristen Maranatha

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.28932/jmh.v5i2.5026

Abstract

Doxorubicin remains the most prescribed anticancer agent despite its unintended side effects on non-target organs. A limiting-dose strategy is used to lower incidence of cardiotoxicity. Andrographolide has therapeutic effects including antioxidant and anti-inflammatory. This study aimed to assess cardioprotective effects of andrographolide oral on lactate dehydrogenase (LDH), creatine kinase-myocardial band (CK-MB), and relative cardiac weight in doxorubicin-induced rats. Sixteen male rats Sprague Dawley randomized into four groups: receives saline i.p and vehicle orally (Normal), doxorubicin 16 mg/kgBW i.p and vehicle orally (Dox), doxorubicin 16 mg/kgBW i.p+andrographolide 30 mg/kgBW orally (Dox+And30), doxorubicin 16 mg/kgBW i.p+andrographolide 60 mg/kgBW orally (Dox+And60). Blood was collected via cardiac puncture and cardiac organs were weighed after four-weeks administration. Total LDH and CK-MB measured spectrophotometrically. LDH and CK-MB levels significantly elevated, and signs of acute toxicity in Dox group compared with Normal group. Co-treatment with andrographolide at 30 mg/kgBW and 60 mg/kgBW reduced signs of toxicity and significantly attenuated LDH and CK-MB levels compared with Dox group (P<0.05 and P<0.01). However, body weight and relative cardiac weight were not significantly different in all groups after co-treatment with andrographolide. In conclusion, andrographolide lowered LDH and CK-MB levels, therefore has a protective potency in alleviating toxic effects of doxorubicin.
(RETRACTION) Efek Centella asiatica terhadap resistensi insulin pada parameter biokimia mencit yang diberi diet tinggi lemak Dwita, Nounik Cheri; Arozal, Wawaimuli; Barinda, Again Jeffilano; Safutra, Sadam
Majalah Kedokteran Andalas Vol. 48 No. 2 (2025): MKA April 2025
Publisher : Faculty of Medicine, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/mka.v48.i2.p164-172.2025

Abstract

Efek Centella asiatica terhadap resistensi insulin pada parameter biokimia mencit yang diberi diet tinggi lemak. 2024; 1422-1431: The above article, published online on January, 2024 in Majalah Kedokteran Andalas, https://doi.org/10.25077/mka.v46.i9.p1434-1443.2024 , has been retracted by agreement between the authors and the journal's Editor in Chief, dr. Hirowati Ali, Ph.D. The retraction has been agreed after the authors asked to retract the article due to a misattribution of authorship.
Andrographis paniculata Ethanol Extract Alleviates High Glucose-induced Senescence of Human Umbilical Vein Endothelial Cells via the Regulation of mTOR and SIRT1 Pathways Khatimah, Nurul Gusti; Arozal, Wawaimuli; Barinda, Agian Jeffilano; Antarianto, Radiana Dhewayani; Hardiany, Novi Silvia; Shimizu, Ippei; Fadhillah, Muhamad Rizqy
The Indonesian Biomedical Journal Vol 16, No 4 (2024)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v16i4.3067

Abstract

BACKGROUND: Chronic exposure of high glucose (HG) in endothelial cell induces senescence which may contribute to the development and progression of age-related diseases including insulin resistance. Andrographis paniculata improves insulin resistance in recent in vitro and in vivo studies. Anti-inflammatory and antioxidant properties of A. paniculata may be the new therapeutic approach to inhibiting premature senescence. However, the senolytic effect of A. paniculata on endothelial cells has not been investigated comprehensively. This study was conducted to evaluate the effect of A. paniculata extract on HG-induced endothelial cell senescence and the underlying mechanisms.METHODS: Human umbilical vein endothelial cells (HUVECs) were treated with 33 mM HG and 7.5 μg/mL A. paniculata extract for 48 hours. The expressions of p16, p21, interleukin (IL)-6, IL-8, insulin receptor substrate (IRS)-1, mammalian target of rapamycin, and sirtuin 1 (SIRT1) were measured by performing real-time quantitative polymerase chain reaction (RT-qPCR). The senescence-associated-β-galactosidase (SA-β-gal) staining was performed to observe the positive-stained senescent cells, while the cell surface expression of IL-1α was examined with flow cytometry method.RESULTS: A. paniculata extract reversed senescence in HUVECs under HG conditions by reducing mRNA expressions of p16 and p21, the number of SA-β-gal-positive-stained cells, and the expression of IL-1α on cell surface, which decreased the activation of IL-6 and IL-8. In addition, A. paniculata extract decreased the mRNA expression of mTOR and increased the mRNA expressions of IRS-1 as well as SIRT1.CONCLUSION: A. paniculata extract ameliorated senescence and improved insulin sensitivity by regulating the mTOR, SIRT1, and IRS-1 mRNA expressions on HG-treated HUVECs.KEYWORDS: Andrographis paniculata, endothelial cell, senescence, high glucose, nutrient-sensing pathways
Optimasi Prosedur Kultur H9C2 Kardiomioblas: Sebuah Pembelajaran untuk Membangun Model Studi In-vitro Kardiovaskular Fadhillah, Muhamad Rizqy; Arozal, Wawaimuli; Wibowo, Heri
EKSAKTA: Journal of Sciences and Data Analysis VOLUME 5, ISSUE 1, April 2024
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/EKSAKTA.vol5.iss1.art8

Abstract

Cardiovascular disease become one of the leading factors of death in the world. Thus, research is urgently needed to discover newer drugs or therapeutical agents and biological plausibility. The H9C2 cardiomyoblast originated from embryonic BDIX rat ventricular cells and was previously used in numerous in vitro studies because of its similar nature to cardiomyocytes. However, to our knowledge, there are still limited studies on the basic procedure for culturing H9C2 cardiomyoblast and arranging the best strategy to perform a suitable timeline. Here, we shared our experience in culturing the H9C2 cardiomyoblast, including harvesting and subculturing the cells. We also demonstrated the change of cell confluency, depending on the seeding number, serum concentration, and culture flask through days 1, 3, and 6, to determine their doubling-time population. H9C2 cardiomyoblasts’ doubling time is around 48-54 days with Mean±SD 2.38±0.41. However, seeding density, different culture flasks, and serum concentration have become independent factors in determining specific measures to harvest the cells for further experiments.
Efek Kombinasi Ekstrak Etanol Acalypha indica dan Centella asiatica pada Jantung Tikus Pascahipoksia: Gen Hif-1a, Troponin I dan Stres Oksidatif Edhiatmi, Marsetyo; Arozal, Wawaimuli; Purwaningsih, Erni H
Jurnal Jamu Indonesia Vol. 1 No. 2 (2016): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v1i2.15

Abstract

Hipoksia meningkatkan pembentukan dan pelepasan spesies oksigen reaktif (ROS). Sel mempunyai mekanisme melindungi diri terhadap kerusakan akibat pembentukan ROS yang terjadi secara alami. Jika pembentukan radikal bebas terjadi berlebihan maka dapat menyebabkan stres oksidatif yang memicu kerusakan sel terutama pada jantung. Sehingga tubuh memerlukan asupan antioksidan. Acalypha indica dan Centella asiatica terbukti memiliki efek antioksidan dan melindungi banyak organ dari kondisi hipoksia, sehingga penelitian ini dilakukan dengan tujuan untuk mengetahui efek antioksidan kombinasi ekstrak etanol Acalypha indica dan Centella asiatica pada organ jantung tikus Spraque-Dawley pascahipoksia. Tiga puluh lima ekor tikus Sprague-Dawley jantan diinduksi hipoksia selama 7 hari dalam ruang khusus, kemudian diberi perlakuan. Ekstrak Acalypha indica, ekstrak Centella asiatica dan kombinasinya diberikan kepada kelompok tikus yang telah dibagi menjadi grup A (hipoksia dan diberi air), B (hipoksia dan diberi kombinasi Acalypha indica 200 mg/kgBB dan Centella asiatica 150 mg/kgBB), C (hipoksia dan diberi kombinasi Acalypha indica 250 mg/kgBB dan Centella asiatica 100 mg/kgBB), D (hipoksia dan diberi Acalypha indica 250 mg/kgBB), E (hipoksia dan diberi Centella asiatica 150 mg/kgBB), F (hipoksia dan diberi vitamin C 100mg/kgBB) dan kelompok normal. Perlakuan diberikan secara oral selama 7 hari setelah hipoksia. Parameter yang diamati adalah ekspresi mRNA HIF-1α, kadar MDA, aktivitas enzim SOD dan ekspresi mRNA cTnI. Tidak terdapat perbedaan bermakna pada ekspresi HIF-1α antara grup A dan kelompok tikus normal (p>0,05). Kadar MDA meningkat signifikan pada grup A (p<0,05) dibanding tikus normal. Kadar MDA grup D mengalami penurunan secara signifikan (p<0,05) dibanding grup A. Aktivitas SOD menurun signifikan pada grup A (p<0,05) dibanding tikus normal. Aktivitas SOD grup B dan E (p<0,05) mengalami peningkatan secara signifikan dibanding grup A. Grup B meningkat signifikan (p<0,05) dibanding grup E. Tidak terdapat perbedaan bermakna antar kelompok perlakuan pada Ekspresi cTnI. Tidak terdapat korelasi antara kadar MDA dan aktivitas SOD serta ekspresi mRNA HIF-1a dan mRNA cTnI. Pemberian kombinasi ekstrak Acalypha indica dan Centella asiatica tidak dapat membantu memproteksi kerusakan jantung pascahipoksia.
Co-Authors Agatha Grace Agian Jeffilano Barinda Andrea Laurentius Arleni Bustami Aryo Tedjo Barinda, Again Jeffilano Bernie Endyarni Medise Cong, Tzeto Han Deni Rahmat Dewi Sukmawati Dewi, Yulia Ratna Djumhana Atmakusuma Djumhana Atmakusuma, Djumhana Dwita, Nounik Cheri Edhiatmi, Marsetyo Erlina, Linda Erni H Purwaningsih Erni H Purwaningsih, Erni H Erni Hernawati Purwaningsih, Erni Hernawati Ernie Purwaningsih Eviarini, Prawitasari Eziefule, Oluebube Magnificient Fadhillah, Muhamad Rizqy Fadilah Fadilah Fadilah, Fadilah Fajarido, Ariski Fatriansyah, Jaka Fajar Femmi Dwinda Agustini Franciscus D. Suyatna Gregorius Bhaskara Wikanendra Gusti Khatimah, Nurul Hanifah Oswari Hedi R. Dewoto Heri Wibowo Hesty Utami Husna, Fauzul Indah D Dewijanti Indah D Dewijanti, Indah D Irawan Mangunatmadja Jusman, Sri Widia Azraki Kapuangan, Christopher Kartika, Kartika Handayani Kenichi Watanabe Khatimah, Nurul Gusti Kuncoro, Daviet Dwi Kurniasari Kurniasari Kusmardi Kusmardi Kusmardi Kusmardi Laurentius, Andrea Lee, Hee Jae Marliana Sri Rejeki Marliana Sri Rejeki Marliana Sri Rejeki Marselina Marselina Marsetyo Edhiatmi Meilei Ma Melva Louisa Monayo, Edwina Rugayah Muhamad Sadam Safutra Musridharta, Eka Nabillah, Deya Adiby Nafrialdi Nafrialdi Nastiti Kaswandani Ni Made Dwi Sandhiutami Novi Silvia Hardiany Nur Azizah Nurwulan Adi Ismaya Pebriani, Mala Pradana, Agrin Febrian Primadhani, Suci Widya Punniyakoti T. Veeraveedu Puspita Eka Wuyung Puspita Eka Wuyung Putri, Rizky Clarinta Radiana Dhewayani Antarianto Radoti Merlin Rani Hendiyani Rani Sauriasari Rani Sauriasari, Rani Rejeki, Marliana Sri Ria Anggraeni Rianto Setiabudy Rianto Setiabudy Riski Amanda, Clara Roro Rukmi Windi Perdani, Roro Rukmi ROS SUMARNY Ros Sumarny Safutra, Muhamad Sadam Safutra, Sadam Sagala, Radoti Merlin SEPTELIA INAWATI WANANDI Sesilia Keban Sesilia Keban, Sesilia Setyo Handryastuti Shimizu, Ippei Silmi Hanifah Soni Siswanto Sumiati, Yati Supraja Dwiyono Suyatna, Franciscus D Syarifah Dewi Thandavarayan, Rajarajan Amirthalingam Tri Yuliani Tri Yuliani, Tri Triana, Novi Tzeto Han Cong Ulfa T Wahyuni Ulya, Nurul Vetnizah Juniantito Vivian Soetikno Vivian Soetikno Wardani, Amanda Saphira Wikanendra, Gregorius Bhaskara Yati Sumiyati