I Gusti Agung Ayu Suartini
Fakultas Kedokteran Hewan, Universitas Udayana

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SOSIALISASI PENYAKIT ZOONOSIS ESCHERICHIA COLI O157:H7 SERTA PELAYANAN KESEHATAN SAPI DI DUSUN LAMPU DESA CATUR KINTAMANI BANGLI I W. Suardana; I.B.N. Swacita; I.N. Suartha; I G.N. Sudisma; M.D. Rudyanto; I.G.M. Krisna Erawan; I.N. Suarsana; I.W. Batan; P.A. Sisyawati Putriningsih; T. Sari Nindia; A.L.T. Rompis; I.N. Mantik Astawa; K. Karang Agustina; I.H. Utama; I.G.A. Suartini; I.M. Sukada; I.K. Suada; A.A.A. Mirah Adi
Buletin Udayana Mengabdi Vol 16 No 2 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (186.738 KB)

Abstract

Ternak sapi yang menderita diare berpeluang besar untuk ditemukan adanya agen zoonosis E. coli O157:H7 mengingat sapi sebagai reservoir utama dari agen tersebut.Transmisi penularan strain bakteri ini ke manusia umumnya terjadi melalui konsumsi daging yang kurang dimasak, produk susu yang tidak dipasteurisasi, air yang terkontaminasi feses. Dusun Lampu sebagai salah satu Dusun di Desa Catur merupakan salah satu daerah potensial untuk pengembangan ternak khususnya sapi sehingga menjadikan program pelayanan kesehatan di wilayah tersebut sangat potensial untuk dilakukan. Hasil kegiatan pengabdian masyarakat berupa sosialisasi penyakit zoonosis E. coli O157:H7 serta pelayanan kesehatan ternak sapi di Dusun ini, memperlihatkan respon positif yang dicirikan dengan cukup banyaknya jumlah ternak yang memperoleh pelayanan yaitu sejumlah 65 ekor sapi dari 35 petani ternak. Jenis pelayanan yang dilakukan meliputi tindakan spraying atau pemberian butox terhadap semua ternak sapi yaitu 65 ekor (100%), disusul dengan pemberian vitamin pada 52 ekor (80%), pemberian obat cacing sebanyak 39 ekor (60%), serta pemberian delladryl pada 1 ekor sapi (1,5%). Hasil ini mengindikasikan bahwa program pengabdian yang dilakukan cukup efektif dapat menyentuh kebutuhan dasar petani ternak, sehingga benar-benar dapat dirasakan manfaatnya.
MOLECULAR MORPHOLOGY OF SNAKE VENOM PROTEIN Trimeresurus insularis AFTER FREEZE DRYING DETECTED BY SCANNING ELECTRON MICROSCOPE Adrianto, Steven; Sari, Tri Komala; Suarsana, I Nyoman; Suartini, I Gusti Ayu Agung
Jurnal Kedokteran Hewan Vol 19, No 1 (2025): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v19i1.41219

Abstract

The high protein content in snake venom make venom samples prone damaged. Freeze drying is a solution to stabilize the protein in snake venom and the addition of excipients such as sugar and surfactant, can prevent negative impacts during freeze drying process. The purpose of this study was to examine the morphology of Trimeresurus insularis (T. insularis) snake venom protein molecules after freeze-drying with the addition of sucrose, Tween 80, and PBS (pH 7.2). In this study, venom from nine T. insularis snakes was used. Before freeze drying, the treated samples were supplemented with sucrose, Tween 80, and PBS (pH 7.2), while, the control sample was only supplemented with PBS (pH 7.2). After freeze drying, the morphology of both samples was observed using a scanning electron microscope at magnification of 100x and 3000x. The result showed that the control sample was damaged and resembled broken glass, whereas the treatment sample, although it also appeared as shattered glass, was more intact and exhibited finer and smaller flakes.
IDENTIFICATION OF GRAM-NEGATIVE BACTERIAL ISOLATES FROM PIG TONSILS BASED ON MORPHOLOGY AND BIOCHEMICAL TEST Yan Agus Dharma Nataswara; I Wayan Suardana; I Gusti Ayu Agung Suartini
Buletin Veteriner Udayana Bul. Vet. Udayana. June 2025 Vol. 17 No. 3
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i03.p29

Abstract

Gram-negative bacteria are microorganisms characterized by significant pathogenic potential and are implicated in a wide range of infections affecting both humans and animals. The tonsils, as lymphoid organs integral to the immune system, can act as niches for bacterial colonization due to their conducive microenvironment for microbial growth. This study aimed to identify Gram-negative bacterial species isolated from porcine tonsils through morphological characterization, oxidase testing, and biochemical profiling using the Methyl Red–Voges-Proskauer (MR-VP) tests, as well as to determine the proportional distribution of the identified bacterial taxa. A total of ten presumptive Gram-negative bacterial isolates, preserved in 30% glycerol, were examined at the Veterinary Public Health Laboratory, Faculty of Veterinary Medicine, Udayana University. The isolates underwent Gram staining, oxidase assay, and MR-VP biochemical testing. The results revealed that seven of the ten isolates (70%) were identified as Pseudomonas spp., while the remaining three isolates (30%) belonged to the family Enterobacteriaceae. All isolates tested positive for the MR test, whereas eight isolates were positive and two negative in the VP test. These findings suggest that porcine tonsils may serve as reservoirs for Gram-negative bacteria, particularly Pseudomonas spp. and members of the Enterobacteriaceae family, which may possess zoonotic potential and thus warrant further surveillance and risk assessment.
Morphology of Erythrocytes and Leukocytes of the Lesser Sunda Islands Pit Viper (Trimeresurus insularis): The Green Coloration from Bali Island and the Blue Coloration from Komodo Island Diza Pakom, Muhammad Sofi; Wandia, I Nengah; Setiasih, Ni Luh Eka; Komala Sari, Tri; Kardena, I Made; Suartini, I Gusti Ayu Agung
Jurnal Sain Veteriner Vol 44, No 1 (2026): April
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.111802

Abstract

Indonesia is a tropical country with a diverse range of herpetofauna, one of which is the Trimeresurus insularis snake, a highly venomous species endemic to Indonesia. This snake is distributed across Java, Bali, West Nusa Tenggara, East Nusa Tenggara, and Wetar Island in Maluku Province. Despite its wide distribution, this species exhibits variations in body coloration. T. insularis from Bali is green, while those from Komodo Island are blue; however, both share the same red tail. This study aims to examine the morphology and morphometry of erythrocytes and leukocytes in T. insularis from different localities and color variations. The research utilized blood smear preparations from six healthy T. insularis individuals—three green coloration from Bali and three blue coloration from Komodo Island—analyzed using a microscope and Optilab Viewer camera. Cell morphometry was measured using ImageJ software. The morphometric measurements of erythrocytes included the length and width of the cytoplasm and nucleus, while leukocyte morphometry covered the cytoplasmic and nuclear diameters of heterophils, basophils, eosinophils, azurophils, lymphocytes, and monocytes. The erythrocyte morphology of T. insularis from both Bali and Komodo Island showed no differences. Morphometric data were analyzed using the Independent-Samples T Test. The results showed significant differences in the length and width of the erythrocyte nucleus, as well as in the cytoplasmic and nuclear diameters of lymphocytes. Meanwhile, no significant differences were found in erythrocyte length and width, as well as in the cytoplasmic and nuclear diameters of heterophils, basophils, eosinophils, and monocytes.