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All Journal Buletin Veteriner Udayana Buletin Udayana Mengabdi Jurnal Veteriner Indonesia Medicus Veterinus Majalah Kedokteran Bandung WARTAZOA Indonesian Bulletin of Animal and Veterinary Sciences Jurnal Kedokteran Hewan

I Gusti Agung Ayu Suartini

Fakultas Kedokteran Hewan, Universitas Udayana

Author-ID : 237263

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Education Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Nilai Hematologi Total Eritrosit dan Kadar Hemoglobin Sapi Bali dengan Pakan Hijauan Organik Anak Agung Ananda Septiarini; Ni Ketut Suwiti; I Gusti Ayu Agung Suartini
Buletin Veteriner Udayana Vol. 12 No. 2 August 2020
Publisher : The Faculty of Veterinary Medicine, Udayana University

Organic forage has become the choice of bali cattle feed in Bali, so it is necessary to know its effect on the total erythrocytes average and hemoglobin levels of bali cattle. This study used ten samples of bali cattle aged 12-18 months. Blood samples were taken through the jugular vein using venoject. Total erythrocyte examination and hemoglobin levels of bali cattle were measured using a Veterinary Hematology Analyzer. The results showed that the mean total of erythrocytes was 6.54x106?l, and the mean hemoglobin level was 12.2 g / dL. Total erythrocytes and hemoglobin levels of Balinese cattle with organic forage were still in the normal range

Aktivitas Biologis Imunoglobulin Yolk Anti Parvovirus Setelah Perlakuan Suhu I Gusti Ayu Agung Suartini; Ni Luh Putu Agustini; Ni Luh Eka Setiasih; Sisyawati Putriningsih; Defi Lega Nurwidana
Buletin Veteriner Udayana Vol. 8 No. 1 Pebruari 2016
Publisher : The Faculty of Veterinary Medicine, Udayana University

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This study aims to determine the effect of temperature on the biological activity of various crude and precipitate specific Immunoglobulin (Ig)Y Canine parvovirus (CPV). Hiperimun serum conducted on Isa Brown chickens injected with antigen CPV. Crude yolk Ig preparations derived from chicken serum without purification while the yolk Ig preparations precipitates obtained by the chicken serum was precipitated with ammonium sulfate and dialyzed. Both types of Ig yolk given treatment temperature 50ºC, 60ºC, 70ºC, and 80ºC for 15 minutes. To test Gel Precipitation Test (AGPT) is performed to determine whether there is a specific IgY CPV in the serum of chickens. Biological activity of both types of Ig detected with Barriers Haemagglutination test (HI). The design used in this study is completely randomized design factorial. The results of this study indicate that the temperature was highly significant on the activities of IgY crude and precipitates. Activities IgY crude and precipitate down to the treatment temperature of 50ºC, 60ºC, 70ºC, and 80ºC. Geometric Mean Titer crude IgY respectively - were 26.67, 26, 25.33, and <2º Unit HI while IgY precipitates are respectively 26.33, 25.67, 24, and <2º Unit HI. Based on the results of this study concluded that the biological activity of crude IgY better than IgY precipitates after treatment of a wide range of temperatures.

SOSIALISASI PENYAKIT ZOONOSIS ESCHERICHIA COLI O157:H7 SERTA PELAYANAN KESEHATAN SAPI DI DUSUN LAMPU DESA CATUR KINTAMANI BANGLI I W. Suardana; I.B.N. Swacita; I.N. Suartha; I G.N. Sudisma; M.D. Rudyanto; I.G.M. Krisna Erawan; I.N. Suarsana; I.W. Batan; P.A. Sisyawati Putriningsih; T. Sari Nindia; A.L.T. Rompis; I.N. Mantik Astawa; K. Karang Agustina; I.H. Utama; I.G.A. Suartini; I.M. Sukada; I.K. Suada; A.A.A. Mirah Adi
Buletin Udayana Mengabdi Vol 16 No 2 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

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Ternak sapi yang menderita diare berpeluang besar untuk ditemukan adanya agen zoonosis E. coli O157:H7 mengingat sapi sebagai reservoir utama dari agen tersebut.Transmisi penularan strain bakteri ini ke manusia umumnya terjadi melalui konsumsi daging yang kurang dimasak, produk susu yang tidak dipasteurisasi, air yang terkontaminasi feses. Dusun Lampu sebagai salah satu Dusun di Desa Catur merupakan salah satu daerah potensial untuk pengembangan ternak khususnya sapi sehingga menjadikan program pelayanan kesehatan di wilayah tersebut sangat potensial untuk dilakukan. Hasil kegiatan pengabdian masyarakat berupa sosialisasi penyakit zoonosis E. coli O157:H7 serta pelayanan kesehatan ternak sapi di Dusun ini, memperlihatkan respon positif yang dicirikan dengan cukup banyaknya jumlah ternak yang memperoleh pelayanan yaitu sejumlah 65 ekor sapi dari 35 petani ternak. Jenis pelayanan yang dilakukan meliputi tindakan spraying atau pemberian butox terhadap semua ternak sapi yaitu 65 ekor (100%), disusul dengan pemberian vitamin pada 52 ekor (80%), pemberian obat cacing sebanyak 39 ekor (60%), serta pemberian delladryl pada 1 ekor sapi (1,5%). Hasil ini mengindikasikan bahwa program pengabdian yang dilakukan cukup efektif dapat menyentuh kebutuhan dasar petani ternak, sehingga benar-benar dapat dirasakan manfaatnya.

Pengaruh Pemberian Laktoferin Sapi Terhadap Berat Badan Dan Leukosit Anak Babi Ghina Monita Pramudhita; I Gusti Ngurah Kade Mahardika; I Gusti Ayu Agung Suartini
Jurnal Veteriner Vol 22 No 3 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Penelitian ini bertujuan untuk mengetahui pengaruh pemberian pakan yang ditambahkan laktoferin sapi terhadap berat badan dan leukosit anak babi Landrace. Penelitian dilakukan pada peternakan komersial di Desa Yeh Gangga, Kecamatan Sudimara, Kabupaten Tabanan. Sebanyak 12 anak babi Landrace dari tiga induk yang berbeda digunakan sebagai sampel, dengan pembagian dua kelompok, yaitu kelompok kontrol dan perlakuan. Kelompok kontrol tidak diberikan perlakuan. Kelompok perlakuan diberikan laktoferin sapi selama 7 hari dengan pemberian satu kali dalam satu hari. Penimbangan berat badan anak babi Landrace dilakukan setiap lima hari, kemudian data dianalisis dengan Student’s T-test. Hasil analisis data periode penimbagan berat badan anak babi umur 10 – 35 hari berpengaruh nyata (P<0,05). Pengabilan darah dilakukan pada post perlakuan kemudian diuji dengan uji hematologi. Data yang diperoleh dianalisis menggunakan Student’s T-test. Hasil penelitian menunjukkan bahwa rerata diferensial leukosit tidak berbeda nyata (P>0,05) antara kelompok kontrol dan kelompok perlakuan. Masing-masing komponen leukosit pada kelompok kontrol maupun kelompok perlakuan berada pada rentang nilai normal. Rerata total leukosit anak babi pasca pemberian laktoferin tidak berbeda nyata (P>0,05) antara kelompok kontrol dan kelompok perlakuan.

Pola Pertumbuhan dan Titik Infleksi Sebagai Dasar Memilih Bibit Anjing Kintamani (GROWTH PATTERN AND POINT OF INFLECTION AS THE BASIS OF THE KINTAMANI DOG SELECTION) I KETUT SUATHA; I Gusti Ayu Agung Suartini; I Putu Sampurna
Jurnal Veteriner Vol 20 No 2 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Kintamani dog growth pattern was observed by measuring the length, circumference and body weight. This study aims to obtain a pattern of growth and determine at what age the growth of kintamani dogs reaches the inflection point (begins to grow slowly) and reaches adult size (begins to stop growing). Data on growth patterns and inflection points of kintamani dogs are useful as a reference for determining the right time for the kintamani dog to be first mated, knowing the existence of abnormalities in growth and nutritional status. The research samples were 90 female kintamani dogs and 90 males aged 0 to 800 days. Measurement of body length and circumference uses a meter, while body weight is measured by digital scales. The growth curve used by the estimator is a sigmoid curve with two parameters: the size of body length, chest circumference and body weight at birth and as an adult. The conclusion of this study is that the pattern of growth in body weight, body length, chest circumference, height of the back limbs and height of the front legs of male and female kintamani dogs has a sigmoid growth pattern. The body size of a female dog has an inflection point or begins to grow slowly and reaches an adult size at a younger age than a male. Female dogs also reach the maximum size earlier than male dogs. The speed of reaching the inflection point on the growth of kintamani dogs were: Chest circumference, front limb height, body length, height of the back leg and body weight. While the speed of achieving adult size were : height of the back leg, body length, height of the front limb, chest circumference and weight.

AMINO ACID SEQUENCE MOTIVE OF OSELTAMIVIR BINDING POCKET IN NEURAMINIDASE PROTEIN OF AVIAN INFLUENZA (H5N1) VIRUS FROM HUMAN AND ANIMAL IN INDONESIA I Gusti Ngurah Kade Mahardika; I Made Sukada; Made Suma Antara; I Gusti Ayu Suartini
Jurnal Veteriner Vol 9 No 4 (2008)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Former finding that avian influenza (AI) virus of H5N1 subtype from Indonesia shows reduced sensitivity against oseltamivir is critically reviewed trough molecular observation of the amino-acid sequence motive of neuraminidase protein (NA) of all H5N1 virus from human and animal in Indonesia available in GeneBank. Amino acid sequence of oseltamivir binding pocket of NA protein on all Indonesian viruses is typical for sensitive virus with a concerved motive of H274, E276, R292 dan N294. Resistance issue could not be explained based on available data.

Aktivitas Invitro Senyawa Antimikroba Streptococcus lactis (INVITRO ACTIVITY ANTIMICROBIAL SUBSTANCE PRODUCED BY STREPTOCOCCUS LACTIS) I Nyoman Suarsana; Maria Bintang; Iwan Harjono Utama; Ni Gusti Agung Ayu Suartini
Jurnal Veteriner Vol 2 No 1 (2001)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Aktivitas Invitro Senyawa Antimikroba Streptococcus lactis (INVITRO ACTIVITY ANTIMICROBIAL SUBSTANCE PRODUCED BY STREPTOCOCCUS LACTIS)

Seroprevalensi Virus Avian Influenza H9N2 pada Ayam Kampung (Gallus domesticus) di Pasar Beringkit, Kabupaten Badung, Bali Brigita Galilea Adu; Messy Saputri Boru Sembiring; Oktryna Hodesi Sibarani; I Gusti Ngurah Kade Mahardika; Ida Bagus Kade Suardana; I Gusti Ayu Agung Suartini; Tjokorda Sari Nindhia
Jurnal Veteriner Vol 21 No 3 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Virus Avian Influenza (Avian Influenza Virus/AIV) subtipe H9N2 (AIV-H9N2) telah menjadi perhatian bagi kesehatan unggas. Virus ini telah dilaporkan di beberapa provinsi di Indonesia. Pasar Beringkit merupakan pasar unggas yang menerima suplai unggas dari berbagai daerah di Bali. Pasar ini menjual berbagai jenis unggas seperti: ayam, itik dan ayam kampung. Penelitian ini bertujuan untuk mengetahui seroprevalensi virus Avian Influenza subtipe H9N2 pada unggas domestik di pasar Beringkit, Kabupaten Badung, Bali. Sebanyak 187 sampel darah dikumpulkan dari tiga kali pengambilan yang berbeda. Serum diambil dari ayam broiler, ayam kampung dan itik yang belum divaksin dan diuji menggunakan Hambatan Hemaglutinasi (Haemagglutination Inhibition/HI). Serum diencerkan lima kali dengan NaCl dan dipanaskan 55oC selama 30 menit sebelum dilakukan pengujian. Hasil pemeriksaan uji HI dianalisis dengan uji statistik Non-parametrik Chi-Square. Hasil penelitian ini menunjukkan bahwa dari 187 sampel serum,43 sampel positif mengandung antibodi AIV-H9N2. Seroprevalensi AIV-H9N2 pada ayam broiler sebesar 15,9% (dari total 63), ayam kampung 35,5% (dari total 62) dan itik sebesar 17,7% (dari total 62). Hasil analisis statistika menunjukkan bahwa sampel yang diambil dari antar spesies dengan tiga kali pengambilan berbeda menunjukkan hasil tidak berbeda nyata. Penelitian ini menunjukkan bahwa unggas domestik di Bali telah terinfeksi AIV-H9N2. Biosekuriti, pengawasan pasar dan vaksinasi efektif dalam mencegah infeksi perlu ditingkatkan. Dampak ekonomi yang disebabkan AIV-H9N2 pada unggas domestik perlu dikaji lebih lanjut.

Karakteristik Protein Plasma Sapi Bali (CHARACTERISTICS OF BALI CATTLE PLASMA PROTEINS) Wahyu Tri Utomo; I Nyoman Suarsana; I Gusti Ayu Agung Suartini
Jurnal Veteriner Vol 18 No 2 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

An study was carried out to determine the physiological data on plasma protein characteristics of Bal cattle. A total of 24 plasma samples of male and female bali cattle were characterized by using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE). The data were presented descriptively. The results showed that on the basis of their estimated molecular weights, at least 14 protein bands (1st to band 14th) were identified with the molecular weights of 963,50 kDa, 530 kDa, kDa 346,82, 124,84 kDa, 89,85 kDa, 68,67 kDa, 54,71 kDa, 37,77 kDa, 20,78 kDa, 16,95 kDa, 16,18 kDa, 15,46 kDa, 12,56 kDa and 10,46 kDa, respectively from the highest to the lowest. Furthermore, plasma protein bands of 14 bali cattle were grouped into five fractions, namely albumin, globulin a1, a2, b, and g. Albumin fraction shown by the band 6th to 14th with a molecular weight of 68,67 to 10,46 kDa respectively. Globulin fraction a1 and a2 shown by the band 5th and 4th with a molecular weight of 89,85 kDa and 124,84 kDa respectively. b- globulin fraction shown by the band 3rd with a molecular weight of 346,82 kDa. g-globulin fraction was shown by the band 1st and 2nd with a molecular weight of 963,50 kDa and 530 kDa respectively. The percentage of area band Bali cattle plasma protein fraction consists of 92% albumin, globulin fraction a2 of 3%, g-globulin of 2%, globulin a1 and b of 1%. It can be concluded that the plasma protein band Bali cattle male and female calves (aged 0-1.5 years), puberty (ages 2-2.5 years) and adults (aged 3-5 years) amounted to 14 protein bands with varying thickness comprises of the five fractions are albumin, globulin a1, a2, b, and g. ABSTRAK Penelitian ini bertujuan untuk mengetahui karakteristik protein plasma sapi bali. Sebanyak 24 sampel plasma sapi bali jantan dan betina dikarakterisasi menggunakan metode sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE). Data hasil penelitian dianalisis secara deskriptif. Hasil penelitian menunjukkan bahwa berdasarkan perhitungan bobot molekul pita protein ke-1 sampai pita ke-14 secara berurutan memiliki bobot molekul yaitu 963,50 kDa, 530 kDa, 346,82 kDa, 124,84 kDa, 89,85 kDa, 68,67 kDa, 54,71 kDa, 37,77 kDa, 20,78 kDa, 16,95 kDa, 16,18 kDa, 15,46 kDa, 12,56 kDa, dan 10,46 kDa. Selanjutnya, 14 pita protein plasma sapi bali dikelompokan menjadi lima fraksi yaitu albumin, globulin a1, a2, b, dan g. Fraksi albumin ditunjukan oleh pita ke-6 sampai pita ke-14 dengan bobot molekul 68,67-10,46 kDa. Fraksi globulin a1 dan a2 ditunjukan oleh pita ke-5 dan ke-4 dengan bobot molekul 89,85 kDa dan 124,84 kDa. Fraksi globulin b ditunjukan oleh pita ke-3 dengan bobot molekul 346,82 kDa. Fraksi globulin g ditunjukan oleh pita ke-1 dan ke-2 dengan bobot molekul 963,50 kDa dan 530 kDa. Persentase luas pita protein plasma sapi bali terdiri dari fraksi albumin 92%, fraksi globulin a2 3%, globulin g 2%, globulin a1 dan b 1%. Dapat disimpulkan bahwa pita protein plasma sapi bali jantan dan betina pedet (umur 0-1,5 tahun), pubertas (umur 2-2,5 tahun), dan dewasa (umur 3-5 tahun) berjumlah 14 pita protein dengan ketebalan bervariasi terdiri dari lima fraksi yaitu albumin, globulin a1, a2, a, dan g.

Aktivitas IgY dan IgG Antitetanus setelah Perlakuan pada Berbagai pH, Suhu dan Enzim Proteolitik I Gusti Ayu Agung Suartini; I Wayan Teguh Wibawan; Maggy T. Suhartono; Supar -; I Nyoman Suarta
Jurnal Veteriner Vol 8 No 4 (2007)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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A study was carried out to find out an alternative method of producing antitetanus antibody (IgY) in chicken and to evaluate its activity at different levels of pH, temperatures and proteolytic enzymes. Antitetanus IgY was produced by immunization of chickens with tetanus toxoid, three times weekly at gradual doses of 100, 200, and 300 Lf, respectively. Serum samples were collected 4 weeks following the last immunization. IgY was purified by ammonium sulfat precipitation and gel filtration chromatography (Sephadex G. 120).The purified IgY was then treated at different levels of temperatures and pH as well as proteolytic enzymes. Commercial antitetanus IgG was used as control. The activities of treated IgY and IgG were tested by enzyme linked immunosorbent assay (ELISA). IgY and IgG activities were significantly reduced at 80ºC and completely destroyed at 90ºC. Treatment with pepsin significantly reduced IgY and IgG whereas trypsin slightly reduced IgY activities and has no effect on IgG activities. IgY and IgG activities were reduced significantly at pH < 3 and and only sightly reduced at pH>10. It is evident that heating at >90oC, pH at <3 and treatment with pepsin significantly reduced IgY activities and it appears that IgG was more resistent to the efect of temperatures, pH and proteolytic enzymes

Co-Authors Adrianto, Steven Agik Suprayogi Aida Lousie Tenden Rompis Amalia, Ainun Rizki Amrulloh, Muhammad Faqih Anak Agung Ananda Septiarini Anita Dwi Handayani Areningrat, Putu Ayutia Brigita Galilea Adu Defi Lega Nurwidana Dewi, Kadek Dyah Utami Dewi, Putu Bulan Sasmita DWI SURYANTO Ferbian Milas Siswanto Ghina Monita Pramudhita Gusti Ayu Yuniati Kencana Hidayah, Dinda Nur I Gusti Agung I Gusti Made Krisna Erawan I Gusti Ngurah Kade Mahardika I Gusti Ngurah Sudisma I Ketut Suada I Ketut Suatha I Made Kardena I Made Sukada I MADE SUMA ANTARA I Nengah Kerta Besung i Nengah Wandia I NYOMAN MANTIK ASTAWA I Nyoman Suarsana I Nyoman Suarta I Nyoman Suartha I Putu Sampurna I Sendow I Wayan Suardana I Wayan Suardana I wayan Teguh Wibawan I.H. Utama I.W. Batan Ida Ayu Sri Candra Dewi Ida Bagus Kade Suardana Ida Bagus Ngurah Swacita Indrawati Sendow Indrawati Sendow IRIANI SETYAWATI Iwan Harjono Utama Kadek Karang Agustina M.D. Rudyanto MAGGY T. SUHARTONO Maratun Janah MARIA BINTANG Messy Saputri Boru Sembiring Ni Ketut Dias Nursanty Ni Ketut Suwiti Ni Luh Eka Setiasih Ni Luh Putu Agustini Ni Luh Watiniasih Ni Made Riska Adnyani Nirhayu, Nirhayu Oktryna Hodesi Sibarani Putu Ayu Sisyawati Putriningsih Putu Oky Astawibawa Risha Catra Pradhany Sabella Ivana Ruslie Setyawati, Iriana Sisyawati Putriningsih Supar - T. Sari Nindia Tjokorda Sari Nindhia TRI KOMALA SARI Wahyu Tri Utomo Yosaphat L.S Kote

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