I Gusti Agung Ayu Suartini
Fakultas Kedokteran Hewan, Universitas Udayana

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SOSIALISASI PENYAKIT ZOONOSIS ESCHERICHIA COLI O157:H7 SERTA PELAYANAN KESEHATAN SAPI DI DUSUN LAMPU DESA CATUR KINTAMANI BANGLI I W. Suardana; I.B.N. Swacita; I.N. Suartha; I G.N. Sudisma; M.D. Rudyanto; I.G.M. Krisna Erawan; I.N. Suarsana; I.W. Batan; P.A. Sisyawati Putriningsih; T. Sari Nindia; A.L.T. Rompis; I.N. Mantik Astawa; K. Karang Agustina; I.H. Utama; I.G.A. Suartini; I.M. Sukada; I.K. Suada; A.A.A. Mirah Adi
Buletin Udayana Mengabdi Vol 16 No 2 (2017)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (186.738 KB)

Abstract

Ternak sapi yang menderita diare berpeluang besar untuk ditemukan adanya agen zoonosis E. coli O157:H7 mengingat sapi sebagai reservoir utama dari agen tersebut.Transmisi penularan strain bakteri ini ke manusia umumnya terjadi melalui konsumsi daging yang kurang dimasak, produk susu yang tidak dipasteurisasi, air yang terkontaminasi feses. Dusun Lampu sebagai salah satu Dusun di Desa Catur merupakan salah satu daerah potensial untuk pengembangan ternak khususnya sapi sehingga menjadikan program pelayanan kesehatan di wilayah tersebut sangat potensial untuk dilakukan. Hasil kegiatan pengabdian masyarakat berupa sosialisasi penyakit zoonosis E. coli O157:H7 serta pelayanan kesehatan ternak sapi di Dusun ini, memperlihatkan respon positif yang dicirikan dengan cukup banyaknya jumlah ternak yang memperoleh pelayanan yaitu sejumlah 65 ekor sapi dari 35 petani ternak. Jenis pelayanan yang dilakukan meliputi tindakan spraying atau pemberian butox terhadap semua ternak sapi yaitu 65 ekor (100%), disusul dengan pemberian vitamin pada 52 ekor (80%), pemberian obat cacing sebanyak 39 ekor (60%), serta pemberian delladryl pada 1 ekor sapi (1,5%). Hasil ini mengindikasikan bahwa program pengabdian yang dilakukan cukup efektif dapat menyentuh kebutuhan dasar petani ternak, sehingga benar-benar dapat dirasakan manfaatnya.
MOLECULAR MORPHOLOGY OF SNAKE VENOM PROTEIN Trimeresurus insularis AFTER FREEZE DRYING DETECTED BY SCANNING ELECTRON MICROSCOPE Adrianto, Steven; Sari, Tri Komala; Suarsana, I Nyoman; Suartini, I Gusti Ayu Agung
Jurnal Kedokteran Hewan Vol 19, No 1 (2025): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v19i1.41219

Abstract

The high protein content in snake venom make venom samples prone damaged. Freeze drying is a solution to stabilize the protein in snake venom and the addition of excipients such as sugar and surfactant, can prevent negative impacts during freeze drying process. The purpose of this study was to examine the morphology of Trimeresurus insularis (T. insularis) snake venom protein molecules after freeze-drying with the addition of sucrose, Tween 80, and PBS (pH 7.2). In this study, venom from nine T. insularis snakes was used. Before freeze drying, the treated samples were supplemented with sucrose, Tween 80, and PBS (pH 7.2), while, the control sample was only supplemented with PBS (pH 7.2). After freeze drying, the morphology of both samples was observed using a scanning electron microscope at magnification of 100x and 3000x. The result showed that the control sample was damaged and resembled broken glass, whereas the treatment sample, although it also appeared as shattered glass, was more intact and exhibited finer and smaller flakes.
IDENTIFICATION OF GRAM-NEGATIVE BACTERIAL ISOLATES FROM PIG TONSILS BASED ON MORPHOLOGY AND BIOCHEMICAL TEST Yan Agus Dharma Nataswara; I Wayan Suardana; I Gusti Ayu Agung Suartini
Buletin Veteriner Udayana Bul. Vet. Udayana. June 2025 Vol. 17 No. 3
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2025.v17.i03.p29

Abstract

Gram-negative bacteria are microorganisms characterized by significant pathogenic potential and are implicated in a wide range of infections affecting both humans and animals. The tonsils, as lymphoid organs integral to the immune system, can act as niches for bacterial colonization due to their conducive microenvironment for microbial growth. This study aimed to identify Gram-negative bacterial species isolated from porcine tonsils through morphological characterization, oxidase testing, and biochemical profiling using the Methyl Red–Voges-Proskauer (MR-VP) tests, as well as to determine the proportional distribution of the identified bacterial taxa. A total of ten presumptive Gram-negative bacterial isolates, preserved in 30% glycerol, were examined at the Veterinary Public Health Laboratory, Faculty of Veterinary Medicine, Udayana University. The isolates underwent Gram staining, oxidase assay, and MR-VP biochemical testing. The results revealed that seven of the ten isolates (70%) were identified as Pseudomonas spp., while the remaining three isolates (30%) belonged to the family Enterobacteriaceae. All isolates tested positive for the MR test, whereas eight isolates were positive and two negative in the VP test. These findings suggest that porcine tonsils may serve as reservoirs for Gram-negative bacteria, particularly Pseudomonas spp. and members of the Enterobacteriaceae family, which may possess zoonotic potential and thus warrant further surveillance and risk assessment.
COMPARISON OF BALB/C MICE IMMUNE RESPONSE WHICH HAS BEEN INDUCED WITH DIFFERENT RABIES VACCINE Putera, Ilham Yusuf Bahniar; Suartini, I Gusti Ayu Agung; Sari, Tri Komala
Buletin Veteriner Udayana Bul. Vet. Udayana. February 2026 Vol. 18 No. 1
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bulvet.2026.v18.i01.p01

Abstract

Rabies is a fatal zoonotic disease that remains incurable, making vaccination the primary strategy for prevention in veterinary and human public health. Although multiple commercial rabies vaccines are widely used, comparative data on their immunogenicity are limited and often inconsistent. This study evaluated and compared the humoral and cellular immune responses induced by three commercially available rabies vaccine brands using a BALB/c mouse model. Ten 20-week-old female BALB/c mice were allocated into treatment groups and immunized accordingly. Blood samples were collected at week 0 (pre-vaccination), week 2, and week 4 post-vaccination to determine specific anti-rabies antibody titers using enzyme-linked immunosorbent assay (ELISA). At the end of the study, spleens were harvested for histopathological examination with hematoxylin–eosin staining to assess cellular immune responses. Antibody titers were analyzed using analysis of variance (ANOVA). All three vaccines induced a significant and comparable increase in antibody titers up to two weeks post-vaccination, with no statistically significant differences among brands. By week 4, a decline in antibody levels was observed across all groups. Histopathological analysis revealed similar activation of cellular immunity among vaccines, characterized by lymphoid proliferation and plasma cell activity, without notable inter-group differences. In conclusion, the three commercial rabies vaccines demonstrated equivalent immunogenicity in inducing both humoral and cellular immune responses in BALB/c mice. These findings suggest that no single vaccine brand was superior, and that rabies vaccination strategies should prioritize operational factors such as schedule adherence, booster administration, and proper vaccination techniques rather than brand selection.  
Morphology of Erythrocytes and Leukocytes of the Lesser Sunda Islands Pit Viper (Trimeresurus insularis): The Green Coloration from Bali Island and the Blue Coloration from Komodo Island Diza Pakom, Muhammad Sofi; Wandia, I Nengah; Setiasih, Ni Luh Eka; Komala Sari, Tri; Kardena, I Made; Suartini, I Gusti Ayu Agung
Jurnal Sain Veteriner Vol 44, No 1 (2026): April
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.111802

Abstract

Indonesia is a tropical country with a diverse range of herpetofauna, one of which is the Trimeresurus insularis snake, a highly venomous species endemic to Indonesia. This snake is distributed across Java, Bali, West Nusa Tenggara, East Nusa Tenggara, and Wetar Island in Maluku Province. Despite its wide distribution, this species exhibits variations in body coloration. T. insularis from Bali is green, while those from Komodo Island are blue; however, both share the same red tail. This study aims to examine the morphology and morphometry of erythrocytes and leukocytes in T. insularis from different localities and color variations. The research utilized blood smear preparations from six healthy T. insularis individuals—three green coloration from Bali and three blue coloration from Komodo Island—analyzed using a microscope and Optilab Viewer camera. Cell morphometry was measured using ImageJ software. The morphometric measurements of erythrocytes included the length and width of the cytoplasm and nucleus, while leukocyte morphometry covered the cytoplasmic and nuclear diameters of heterophils, basophils, eosinophils, azurophils, lymphocytes, and monocytes. The erythrocyte morphology of T. insularis from both Bali and Komodo Island showed no differences. Morphometric data were analyzed using the Independent-Samples T Test. The results showed significant differences in the length and width of the erythrocyte nucleus, as well as in the cytoplasmic and nuclear diameters of lymphocytes. Meanwhile, no significant differences were found in erythrocyte length and width, as well as in the cytoplasmic and nuclear diameters of heterophils, basophils, eosinophils, and monocytes.
Co-Authors Adrianto, Steven Agik Suprayogi Aida Lousie Tenden Rompis Amalia, Ainun Rizki Amrulloh, Muhammad Faqih Anak Agung Ananda Septiarini Anita Dwi Handayani Areningrat, Putu Ayutia Brigita Galilea Adu Defi Lega Nurwidana Dewi, Kadek Dyah Utami Dewi, Putu Bulan Sasmita Diza Pakom, Muhammad Sofi DWI SURYANTO Ferbian Milas Siswanto Ghina Monita Pramudhita Gusti Ayu Yuniati Kencana Hidayah, Dinda Nur I Gusti Agung I Gusti Made Krisna Erawan I Gusti Ngurah Kade Mahardika I Gusti Ngurah Sudisma I Ketut Suada I Ketut Suatha I Made Kardena I Made Sukada I MADE SUMA ANTARA I Nengah Kerta Besung i Nengah Wandia I NYOMAN MANTIK ASTAWA I Nyoman Suarsana I Nyoman Suarta I Nyoman Suartha I Putu Sampurna I Sendow I Wayan Suardana I Wayan Suardana I Wayan Suardana I wayan Teguh Wibawan I.H. Utama I.W. Batan Ida Ayu Sri Candra Dewi Ida Bagus Kade Suardana Ida Bagus Ngurah Swacita Indrawati Sendow Indrawati Sendow IRIANI SETYAWATI Iwan Harjono Utama Kadek Karang Agustina M.D. Rudyanto MAGGY T. SUHARTONO Maratun Janah MARIA BINTANG Messy Saputri Boru Sembiring Ni Ketut Dias Nursanty Ni Ketut Suwiti Ni Luh Eka Setiasih Ni Luh Putu Agustini Ni Luh Watiniasih Ni Made Riska Adnyani Nirhayu, Nirhayu Oktryna Hodesi Sibarani Putera, Ilham Yusuf Bahniar Putu Ayu Sisyawati Putriningsih Putu Oky Astawibawa Risha Catra Pradhany Sabella Ivana Ruslie Setyawati, Iriana Sisyawati Putriningsih Supar - T. Sari Nindia Tjokorda Sari Nindhia TRI KOMALA SARI Wahyu Tri Utomo Yan Agus Dharma Nataswara Yosaphat L.S Kote