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Efektivitas Ekstrak Daun Komak (Lablab purpureus) Terhadap Sistem Imun Ikan Nila (Oreochromis niloticus) Yang Diinjeksi Bakteri Aeromonas hydrophila Rabi’atussamiah; Azhar, Fariq; Abidin, Zaenal; setyowati, Dewi Nur’aeni
Jurnal Media Akuakultur Indonesia Vol 4 No 4 (2024): Indonesian Journal of Aquaculture Medium
Publisher : Program Studi Budidaya Perairan Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/mediaakuakultur.v4i4.6140

Abstract

This study aims to determine the effect of comma leaf extract on the immune system of tilapia injected with A. hydrophila bacteria. This research was conducted for 60 days at the Fish Production and Reproduction Laboratory and Aquaculture Fish Health Laboratory, Aquaculture Study Program, Department of Fisheries and Marine Sciences, Faculty of Agriculture, University of Mataram. The method used was a completely randomized design (CRD) experiment with five treatments and three replications, namely where K- was not given comma leaf extract and was injected with 0.9% NaCl, K+ was not given comma leaf extract and was injected with A. Hydrophila bacteria, P1 (0.5%) extract comma leaf and injected with A. hydrophila bacteria, P2 (1%) of komak leaf extract and injected with A. hydophila bacteria, P3 (2%) of komak leaf extract and injected with A. hydrophila bacteria. The observed parameters were total erythrocytes, total leukocytes, hemoglobin, hematocrit, differential leukocytes, phagocytosis activity, total bacteria and survival (SR). The results showed that the average value of total erythrocytes was 1.22-1.82 x106 cells/mm3, total leukocytes were 2.08-2.8 x104 cells/mm3, hemoglobin was 3.4-5.5%, hematocrit was 14.1-17.3%, differential leukocytes consisted of 4 types of cells, namely lymphocytes. , monocytes, neutrophils and platelets. Lymphocytes 60.7-72.3%, monocytes 4.3-7.3%, neutrophils 17-24.3%, and platelets 6.3-7.7%, phagocytosis activity 44.8-66.3%, total bacteria (TB) OD620 3.39-3.84, survival (SR) 46.7-66.7 %. The addition of 2% of comma leaf extract to the feed can improve the immune system of tilapia injected with A. hydrophila bacteria
The Effect of Adding White Tumeric (Curcuma zedoaria) Extract on the Immune System of Vaname Shrimp (Litopenaeus vannamei) Tested Against Vibrio harveyi Bacteria Rosyida, Azilia; Setyowati, Dewi Nur’aeni; Azhar, Fariq
Jurnal Perikanan dan Kelautan Vol. 27 No. 2 (2022): June
Publisher : Fakultas Perikanan dan Kelautan Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/

Abstract

The entry of vaname shrimp into Indonesia was to replace tiger prawns (Penaeus monodon) which at that time experienced a decline in quality. Although vaname shrimp is famous for having various advantages, there are still obstacles encountered during the cultivation period, one of which is the attack of vibriosis caused by the bacterium Vibrio harveyi. This study aims to determine the effect of adding white turmeric extract (Curcuma zedoaria) on the immune system of white shrimp (Litopenaeus vannamei) which was challenged with Vibrio harveyi bacteria. This research was conducted for 60 days from March to May 2021 using experimental research methods. The research container used a container container (size 40x30x28 cm) and a stocking density of 20 fish/container with a volume of 20 L. The research design used was a completely randomized design consisting of 5 treatments with 3 replications where P1: artificial feed + Vibrio harveyi infection, P2: artificial feed + 0.1 mL NaCl injection, P3: artificial feed + 0,5% white turmeric extract + V.harveyi infection, P4: Artificial Feed + 1% white turmeric extract + V.harveyi infection, P5: Artificial feed + 2% white turmeric extract + V.harveyi infection. The results showed that administration of white turmeric extract (C.zedoaria) with a dose of 1% gave the best results and had a significant effect on the survival rate of 68.3%, Total Haemocyte Count of 21.63x10⁶ cells/mL, and Differential Haemocyte Count (hyaline cells). by 58.7%, semi-granulocytes by 18.33%, granulocytes by 23%) which play a role in the immune system of white vaname shrimp. In addition, the value of phagocytic activity was 68.8% and was able to suppress the growth of bacteria in the intestine at the lowest at 57.7×108 CFU/mL and suppress the number of vibrios to the lowest number at 26×106 CFU/mL.
Effect of Frequency of Administration of Kappa-Carrageenan on Immune Response and Survival of Vibrio parahaemolyticus Infected Vannamei Shrimp (Litopenaeus vannamei) Febrianti, Baiq Yunita; Azhar, Fariq; Mukhlis, Alis
Jurnal Biologi Tropis Vol. 25 No. 3 (2025): Juli-September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i3.9825

Abstract

Whiteleg shrimp (Litopenaeus vannamei) cultivation activities are often exposed to disease-causing pathogens, one of which is a bacterial disease from the genus Vibrio sp. One of the triggers is the accumulation of organic matter, leftover feed and feces in the waters which causes whiteleg shrimp to be toxic to NH3. Kappa carrageenan is a polysaccharide compound derived from red seaweed that has the potential as a natural immunostimulant. The study aimed to determine the effect of administering kappa carrageenan at different frequencies on the defense system of shrimp infected with Vibrio parahaemolyticus bacteria. The study used RAL, with 5 treatments and 3 replications. The highest Survival Rate (SR) value was found in P5 at 80% and the lowest in P3 at 22%. The highest RPS value was found in P5 at 94.82% which was given carrageenan once a week and the lowest in P3 at 82.18% which was given carrageenan every day. The highest THC amount was observed in P5 at 14.9 x 106 cells/ml and the lowest was in P1 at 5.01 x 106 cells/ml. The highest AF value was in the positive control treatment P1 66.6% and the lowest P2 negative control 44.2%. The highest TBC value was P1 1.24 CFU/mL and the lowest P5 0.29 CFU/mL. The highest TVC observation was P5 1.42 CFU/mL and the lowest was P1 0.18 CFU/mL. In conclusion, the administration of k-carrageenan 20 g/kg once every 7 days provided a better immune response and protection against shrimp infected with Vibrio parahaemolyticus bacteria.
Enhancing ornamental marine fish farming through advanced water management with protein skimmers in Lembar, West Lombok Affandi, Rangga Idris; Azhar, Fariq; Rahmadani, Thoy Batun Citra; Fitria, Syawalina; Agustin, Pradita Ayu; Setyono, Bagus Dwi Hari; Diamahesa, Wastu Ayu; Diniariwisan, Damai; Sumsanto, Muhammad; Abidin, Zaenal
Penamas: Journal of Community Service Vol. 5 No. 3 (2025): Penamas: Journal of Community Service
Publisher : Nur Science Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53088/penamas.v5i3.2021

Abstract

The coastal area of Lembar District, West Lombok, has great potential in cultivating marine ornamental fish such as clownfish and blue tang, but water pollution due to Lembar Port activities has caused a decline in water quality and low fish survival. This community service aims to increase the capacity of farmers in managing water quality through the application of protein skimmer technology, a tool that can remove dissolved organic particles from seawater. The activity was carried out in six stages, namely location survey, socialization, training, technology application, mentoring and evaluation, and program sustainability. The results of the activity showed that partners experienced an increase in understanding of the importance of water quality and skills in using protein skimmers. In addition, the condition of the cultivation environment became more stable and supported the growth of ornamental fish. With a participatory approach and the application of appropriate technology, this activity was able to provide a positive impact. This program is expected to be a pilot model for other coastal areas in the development of sustainable marine ornamental fish cultivation.
PELATIHAN BUDIDAYA NILA KOLAM TERPAL DI DESA GRIMAK, LOMBOK BARAT UNTUK MENCEGAH STUNTING Muahiddah, Nuri; Azhar, Fariq; Affandi, Rangga Idris; Diniariwisan, Damai; Diamahesa, Wastu Ayu
Jurnal Pepadu Vol 5 No 3 (2024): Jurnal PEPADU
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/pepadu.v5i3.5909

Abstract

Masalah stunting masih menjadi tantangan serius di Desa Grimak Indah, Lombok Barat, dengan prevalensi yang cukup tinggi di kalangan anak-anak. Upaya pencegahan stunting melalui peningkatan asupan gizi sangat diperlukan, salah satunya melalui pemanfaatan sumber protein yang terjangkau dan mudah diakses oleh masyarakat setempat. Oleh karena itu, program pengabdian ini bertujuan untuk memberikan pelatihan budidaya ikan nila dengan menggunakan kolam terpal sebagai solusi praktis dan ekonomis untuk meningkatkan ketahanan pangan dan asupan protein di desa tersebut. Kegiatan pelatihan meliputi berbagai aspek teknis budidaya ikan nila, seperti persiapan dan pembuatan kolam terpal, pemilihan bibit unggul, teknik pemberian pakan, serta pengelolaan kualitas air dan kesehatan ikan. Selain itu, program ini juga memberikan edukasi mengenai pentingnya konsumsi protein hewani dalam pencegahan stunting, serta bagaimana memanfaatkan hasil budidaya secara optimal. Hasil dari pelatihan ini diharapkan dapat meningkatkan pengetahuan dan keterampilan masyarakat Desa Grimak Indah dalam budidaya ikan nila, sehingga dapat memperkuat ketahanan pangan keluarga dan berkontribusi dalam upaya pencegahan stunting di wilayah tersebut. Keberhasilan program ini diukur melalui peningkatan jumlah kolam terpal yang dibangun dan produksi ikan nila yang stabil, serta peningkatan kesadaran masyarakat akan pentingnya asupan gizi yang seimbang.
Pengaruh Pemberian Pakan dari Limbah Penetasan Telur Ayam terhadap Media Pemeliharaan Lobster Pasir (Panulirus Homarus) Amanda, Dwika Melani; Azhar, Fariq; Scabra, Andre Rachmat; Syukur, Abdul; Amin, Muhammad; Faturrahman, Faturrahman
JURNAL SAINS TEKNOLOGI & LINGKUNGAN Vol. 10 No. 1 (2024): JURNAL SAINS TEKNOLOGI & LINGKUNGAN
Publisher : LPPM Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jstl.v10i1.604

Abstract

Chicken egg hatching waste flour can be an alternative to replace expensive spiny lobster feed because apart from being waste it is also cheap and easy to obtain. The purpose of this research is to determine the effect of feeding chicken egg hatching waste on the water quality of spiny lobster (Panulirus homarus). This research used an experimental method with a Completely Randomized Design (CRD) consisting of 4 treatments with 3 replication. The results of the study showed that the composition of chicken egg waste can influence temperature, DO, pH, salinity, nitrate, nitrite, ammonia, absolute weight growth, absolute length growth, Specific Growth Rate, Feed Conversion Ratio and Survival Rate of spiny lobste.
Effectiveness of Use of UV Lamp in Disinfection of Additioned Vaname Shrimp (Litopenaeus vannamei) Cultivation Media Bacteria Vibrio Harveyi Hapizah, Imro'atul; Junaidi, Muhammad; Azhar, Fariq
Jurnal Biologi Tropis Vol. 24 No. 2 (2024): April - Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i2.6889

Abstract

One of the most promising fisheries products in Indonesia is vaname shrimp (Litopenaeus vannamei), which is also a top export comodity. From year to year the value of shrimp exports and production continues to increase. However, vaname shrimp are susceptible to bacterial and viral attacks, so countermeasures are needed, one of which is by disinfecting the cultivation media using a UV lamp. The aims of this research is to determine the level of effectiveness of UV lamps in disinfection of cultivation media. Experimental methodology with a completely randomized plan (RAL) is the method used in this research, with 4 trials and 3 replications. The results of the research conducted showed that UV lamps were able to reduce the total bacterial count to 4.69 x 108 CFU/mL at UV lamp 41 watt, reduce the total Vibrio bacteria to 30.33 x 106 CFU/mL at UV lamp 41 watt, increase the THC value to 8.83 x 106 CFU/mL cells/ml at P4, hyaline cells range from 31% – 54.67%, granulocyte cell values ​​range from 29.33% – 42%, semigranulocyte cells range from 16% – 27%, the highest AF value at P4 is 32.19%, and the lowest in P1 was 79.69%. Based on the findings, it is possible to conclude that UV lights can be used as a disinfectant of cultivation media, but the right dose or light power is needed to get maximum results.
The Effect of Giving Mangrove Leaf Extract (Rhizopora apiculata) on The Immune System of Goldfish (Cyprinus carpio) Infected with Aeromonas hydropila Bacteria Maknun, Lu'lu'il; Azhar, Fariq; Mulyani, Laily
Jurnal Biologi Tropis Vol. 24 No. 1b (2024): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i1b.7780

Abstract

Goldfish (Cyprinus carpio) is a type of freshwater fishery commodity whose production activities are currently growing rapidly because they are in great demand by the public. Giving Rhizopora apiculate mangrove leaf extract can reduce the number of bacteria Vibrio in vannamei shrimp so that Rhizopora apiculate mangrove leaves can be used as a natural immunostimulant. The aim of this research is to determine the effect of administering Rhizopora apiculata mangrove leaf extract at different doses on the immune system of goldfish infected with Aeromonas hydrophila bacteria. This research was carried out for 60 days at the Fish Production and Reproduction Laboratory, Aquaculture Study Program, Mataram University. The method in this study used a completely randomized design with 5 treatments and 3 repetitions. The results of the study showed that P2 was the best result that was able to improve the immune system of young mothers after the challenge test, this can be seen from the erythrocy value obtained at 5.41 x 106 cells/mm3, the leukocyte value at 6.06 x 104 cells/mm3, hemoglobin 10.7%, hematocrit 21.2%, lymphocytes 73.3%, monocytes 37.3%, neutrophils 32.3%, platelets 36.6% and phagocytic activity 34, 5%. The conclusion from the research that has been carried out is that the addition of R. apiculta mangrove leaf extract to goldfish feed has a significantly different effect on the erythrocyte, leukocyte and hematocrit levels of goldfish (C. carpio). The addition of mangrove leaf extract at a dose of 1% to goldfish is the right dose which can improve the body's defense system in goldfish.
Effectiveness of Mangosteen Peel Extract Garcinia mangostana in Inhibiting Aeromonas hydrophila Bacteria Muahiddah, Nuri; Azhar, Fariq; Rachmat Scabra , Andre; Idris Affandi, Rangga; Dwiyanti , Septiana
Jurnal Biologi Tropis Vol. 24 No. 1b (2024): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i1b.7936

Abstract

The aquaculture sector is growing rapidly, but it faces various challenges, particularly concerning the health of fish and other aquatic organisms.This study aims to evaluate the potential of mangosteen peel extract as an antimicrobial agent against the bacterium Aeromonas hydrophila. This bacterium is known as a pathogen in fish and can cause significant diseases in aquaculture. Mangosteen peel extract was chosen because it contains active compounds such as xanthones, which have been proven to have antimicrobial properties. The initial method involves extraction with methanol, followed by filtration and separation using an evaporator. The method used to determine the content of mangosteen peel extract is FTIR analysis. Next, the antibacterial activity of the crude mangosteen peel extract is tested. The antioxidant testing is based on the agar diffusion method. Aeromonas hydrophila bacteria are cultured at a tested LD50 density of 0.05 ml on a TSA plate medium, spread evenly across the entire agar surface with a glass stick. Paper discs with a diameter of 6 mm, previously dipped in crude mangosteen peel extract, are placed on different TSA media. The test is repeated three times (triplicate) to obtain the average inhibition zone (clear zone), then incubated at 29°C, and observations are made of the inhibition zone's development at the 24th hour. The inhibition zone of mangosteen peel extract (Garcinia mangostana) against Aeromonas hydrophila bacteria, measuring 0.2±0.0 cm (20 mm), falls into the category of very strong. This indicates that mangosteen peel has the potential to be used as an alternative immunostimulant in place of antibiotics for the control of Motile Aeromonas Septicemia.
The Effect of The Solvent-To-Leaf Ratio in The Extraction Process of Moringa leaves on Erythrocyte and Lecocyte of Tilapia Yadin, Yadin; Abidin, Zaenal; Azhar, Fariq
Jurnal Biologi Tropis Vol. 25 No. 1 (2025): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i1.8265

Abstract

Tilapia (Oreochromis niloticus) is a freshwater aquaculture fish that has a fairly high economic value among the Indonesian people. The purpose of this study was to determine the effect of the ratio between the number of moringa leaves and the extracting solution in the extraction process on total lekocytes and total erythrocyte of tilapia. The research was designed in a completely randomized design (CRD) with 5 treatments, namely the comparison of the number of moringa leaves and water solvents. The treatments are control (A), without extract), moringa leaves 5 gr + 10 ml solvent (B), moringa leaves 40 gr + 10 ml solvent (C), moringa leaves 70 gr + 10 ml solvent (D), and moringa leaves 100 gr + 10 ml solvent (E). Each solution was added to the feed as much as 50 ml / 30 grams and given to the fish for 16 days. Erythrocytes and leukocytes were counted on day 0, day 1, day 2, day 3, day 7 and day 16. The results showed that the addition of moringa leaf extract solution in feed affect (P<0.05) the total erythrocytes and total leukocytes in the first day until the 16th day of administration. The best observation results were shown in the addition of 5 g moringa leaf extract solution dissolved with 10 ml of water.