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All Journal Jurnal Kesehatan Andalas Jurnal Kesehatan Prima Jurnal Mandala Pharmacon Indonesia Jurnal Analis Medika Biosains (JAMBS) Jurnal Pengabdian Masyarakat Multidisiplin Jurnal Pengabdian Masyarakat Sasambo Eduvest - Journal of Universal Studies Jurnal Kefarmasian Indonesia Journal of Indonesia Laboratory Students (JILTS) Jurnal Indonesia Mengabdi Galen: Jurnal Ilmu Farmasi dan Kesehatan
Agrijanti, Agrijanti
Poltekkes Kemenkes Mataram, Mataram, Nusa Tenggara Barat
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Journal : Jurnal Kesehatan Prima

 1 
COLIFORM MOST PROBABLE NUMBER (MPN) WITH VARIETIES OF MEDIA VOLUME LACTOSE BROTH SINGLE STRENGTH (LBSS) AND LACTOSE BROTH DOUBLE STRENGTH (LBDS) Yunan Jiwintarum; Agrijanti Agrijanti; Baiq Lilis Septiana
Jurnal Kesehatan Prima Vol 11, No 1 (2017): Jurnal Kesehatan Prima
Publisher : poltekkes kemenkes mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (0.272 KB) | DOI: 10.32807/jkp.v11i1.17

Abstract

Most Probable Number (MPN) is a test that detects the fermentative nature of Coliform in the sample. The MPN test consists of three stages: presumptive test, confirm test, and completeness test. Each of these tests used LBSS, LBDS and BGLB media with volume between 5 ml - 10 ml. There is the variation of this media volume, whether it will affect the positivity value and MPN value of Coliform, it is a problem that will be answered in this study. The research objective was to find out the influence of media volume variation to the result of Coliform bacterium counting test method of MPN variety 5 1 1. The media volume which was varied namely LBSS and LBDS with volume variation 4 m, 6 ml, 8 ml, 10 ml, 12 Ml. The research was an experimental in laboratory. The sample was the suspense of Escherichia coli bacteria which are equivalent to standard 0.5 Mc. Farland and diluted 1000 ml sterile aquadest, the result data indicated MPN value was obtained, then it is analyzed by descriptive analysis. The findings indicated that the volume of LBDS and LBSS media were 4ml, 6ml, 8ml, 10ml, 12ml, replicated 5 times obtained positive result on all tubes 5 1 1 with MPN value> 265/100 ml. The conclusion of the research is media volume of LBSS and LBDS does not affect the positive and MPN Coliform values.
The Effect of Roasting Methods of Ingredients The Songgak Tribe Sasak Herbal on Active Compounds Using Gas Chromatography Mas Spectroscopy (Gc-Ms) Analysis) Agrijanti, Agrijanti; Kusuma Dewi, Lale Budi; Khusuma, Ari; Wardoyo, Eustachius Hagni
Jurnal Kesehatan Prima Vol 18, No 2 (2024): AUGUST
Publisher : poltekkes kemenkes mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jkp.v18i2.1540

Abstract

Songgak is a concoction of the Sasak tribe of Lombok in West Nusa Tenggara. It consists of coffee mixed with groundwood, cloves, black pepper, coriander, and nutmeg. The original recipe involves roasting the spices before mixing them with coffee, namely using a frying pan over a stove. The roasting process is carried out without controlling the temperature and roasting time, so the roasted ingredients need to be standardized(Dewi et  al., 2021). GCMS is a tool used to analyze compounds in samples using chemical separation methods, mainly chromatography. The compounds in the mixture are separated in a chromatographic column. The separation mechanism between several compounds occurs due to differences in the solubility values of each in the moving solvent and differences in the absorption of each of the competing compounds into the stationary phase (Gross, 2017). In this research, variations will be carried out on spices, namely T1 original treatment of roasting spices over fire for 8 minutes, T2 drying spices in sunlight, T3 oven for 5 minutes, T5 oven for 5 minutes, T4 oven for 15 minutes and T5 oven for 15 minutes. In the T4 treatment, namely oven for 10 minutes, the results of the analysis of the substance content with the most types, namely 20 types, were obtained. The active anti-microbial compound found in all treatments was Hexadecanoic acid, 2-hydroxy-1-(h)(C19 H38 O4) BM:330, which helps inhibit the growth of cancer cells. (National Library Medicine, n.d.). Octadecanoic acid (CAS) Stear (C18 H34 O2) BM:282 Anti-inflammatory, Antiandrogenic Cancer prevention, Hypocholesterolemia Dermatigenic, 5-Alpha reductase inhibitor, Anemiagenic, Insecticide (Natarajan et al., 2019) and Tetradecanoic acid (CAS) Myris (C14 H28 O2) BM:228 as anti-inflammatory and anti-nociceptive. 
The Effect of Roasting Methods of Ingredients The Songgak Tribe Sasak Herbal on Active Compounds Using Gas Chromatography Mas Spectroscopy (Gc-Ms) Analysis) Agrijanti, Agrijanti; Kusuma Dewi, Lale Budi; Khusuma, Ari; Wardoyo, Eustachius Hagni
Jurnal Kesehatan Prima Vol. 18 No. 2 (2024): AUGUST
Publisher : poltekkes kemenkes mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jkp.v18i2.1540

Abstract

Songgak is a concoction of the Sasak tribe of Lombok in West Nusa Tenggara. It consists of coffee mixed with groundwood, cloves, black pepper, coriander, and nutmeg. The original recipe involves roasting the spices before mixing them with coffee, namely using a frying pan over a stove. The roasting process is carried out without controlling the temperature and roasting time, so the roasted ingredients need to be standardized(Dewi et  al., 2021). GCMS is a tool used to analyze compounds in samples using chemical separation methods, mainly chromatography. The compounds in the mixture are separated in a chromatographic column. The separation mechanism between several compounds occurs due to differences in the solubility values of each in the moving solvent and differences in the absorption of each of the competing compounds into the stationary phase (Gross, 2017). In this research, variations will be carried out on spices, namely T1 original treatment of roasting spices over fire for 8 minutes, T2 drying spices in sunlight, T3 oven for 5 minutes, T5 oven for 5 minutes, T4 oven for 15 minutes and T5 oven for 15 minutes. In the T4 treatment, namely oven for 10 minutes, the results of the analysis of the substance content with the most types, namely 20 types, were obtained. The active anti-microbial compound found in all treatments was Hexadecanoic acid, 2-hydroxy-1-(h)(C19 H38 O4) BM:330, which helps inhibit the growth of cancer cells. (National Library Medicine, n.d.). Octadecanoic acid (CAS) Stear (C18 H34 O2) BM:282 Anti-inflammatory, Antiandrogenic Cancer prevention, Hypocholesterolemia Dermatigenic, 5-Alpha reductase inhibitor, Anemiagenic, Insecticide (Natarajan et al., 2019) and Tetradecanoic acid (CAS) Myris (C14 H28 O2) BM:228 as anti-inflammatory and anti-nociceptive. 
Co-Authors Aeni Halawiya Alfiah Novianty Anisa Prisilia Prisilia Ari Khusuma azwa rafiza Baiq Lilis Septiana Cahayaningrum, Aryani Dewi, Lale Budi Kusuma Dwi Melbaow Aisyiah Putri Erlin Yustin Tatontos Erna Kristinawati Erna Kristinawati Eustachius Hagni Wardoyo Fadhilah Rizkiyah Febriza Marta Utami Fihiruddin, Fihiruddin Getas, I Wayan I Made Wahyu Waisnawa I Wayan Getas I Wayan Getas Imanta Alifia Octavira Intan Suryawatie Yomo, Meidiana Jasmine Chairunnisa Lalu Srigede Lalu Srigede Lalu Srigede, Lalu Lina Sundayani Meidiana Intan Suryawatie Yomo Muhammad Luthfi Ni Komang Ari Santi Widyastini Nispahayati Nispahayati Nova Ramdhani, Iid Nirmala Novianty, Alfiah Nurhidayati Nurhidayati Nurul Inayati Nurul Inayati Nurul Inayati Nurul Inayati Octavira, Imanta Alifia Pancawati Ariami Permatasari, Lina Putriawati Putriawati Putriawati, Putriawati RAI WIADNYA, IDA BAGUS Rohmi Rohmi Rohmi Rohmi Rohmi Rohmi Rohmi Rohmi, Rohmi Siti Karyani Siti Zaetun Siti Zaetun Siti Zaetun Siti Zaetun, Siti Sri Hartuti Sudirman Halla Sufina Zikria Putri Supriyanti, Ispi Thomas Tandi Manu Vivi Oktapia Wahyuni, Leni Waisnawa, I Made Wahyu Widyastini, Ni Komang Ari Santi Wulandari, Ayu Nurislami Yudha Anggit Jiwantoro Yuliana Tri Risky Yunan Jiwintarum Zahira, Jauhara Rana