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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Agrikultura Microbiology Indonesia Jurnal Hortikultura JURNAL AGROTEKNOLOGI Jurnal Biologi Universitas Andalas Jurnal Agroekoteknologi Jurnal Perlindungan Tanaman Indonesia Jurnal Ilmiah Universitas Batanghari Jambi Agrosains: Jurnal Penelitian Agronomi Jurnal Pertanian Agros Journal of Applied Agricultural Science and Technology Al Qalam: Jurnal Ilmiah Keagamaan dan Kemasyarakatan JURNAL AGROHITA JURNAL PERTANIAN JERAMI Indonesian Journal of Crop Science Menara Perkebunan Menara Ilmu Jurnal Abdi Masyarakat (JAM) Agro Bali: Agricultural Journal Jurnal Abdi Insani Warta Pengabdian Andalas: Jurnal Ilmiah Pengembangan Dan Penerapan Ipteks Journal of Global Sustainable Agriculture International Journal of Science and Society (IJSOC) Jurnal Riset Perkebunan (JRP) Journal of Applied Agricultural Science and Technology Jagur: Jurnal Agroteknologi Buletin Dharmas Andalas
Anwar, Aswaldi
Fakultas Pertanian, Universitas Andalas
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Journal : Menara Perkebunan

 1 
Kloning gen LEAFY kakao dari jaringan bantalan bunga aktif Cloning of cacao LEAFY gene from the active flower cushions Tetty CHAIDAMSARI; Rita HAYATI; Auzar SYARIEF; Aswaldi ANWAR; Djoko SANTOSO
E-Journal Menara Perkebunan Vol 77, No 2: Desember 2009
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (215.668 KB) | DOI: 10.22302/iribb.jur.mp.v77i2.179

Abstract

SummaryAttempts to improve productivity of cacaoplantations lead us to study the molecularmechanism of flowering. In the model speciesArabidopsis thaliana as well as some otherspecies, LEAFY is a central regulatory gene forthe transition of shoot apical meristems toflowering meristems. Different from that ofArabidopsis, cacao inflorescence is acauliflorous type, by which flowers can developrepeatedly from the same flower cushion on thetrunk. In this research, a LEAFY homolog wasisolated from active flower cushion with RT-PCRusing a pair of DNA primer specifically designedto isolate its complete cds. Gel electrophoresisexamination indicated the presence of a 1.2 kbamplicon. Purified from the gel, this DNAfragment was cloned into competent cells ofE. coli XL1 Blue using pGEM-T Easy cloningvector at an orientation according to the T7promoter of the plasmid. Sequence analysis usingBLASTX, showed that the amplicon was LEAFY(LFY) homolog. Alignment analysis using ClustalW indicated that the cTcLFY highly homologousto those from other perennial crops such ascitrus, grape, apple and poplar. The highesthomology (conserved region) was found in the Cterminal of the encoded proteins.RingkasanUsaha untuk meningkatkan produktivitasperkebunan kakao telah mendorong penelitianmolekuler tentang mekanisme pembungaankakao. Pada tanaman model Arabidopsis thalianadan lainnya, LEAFY merupakan gen kunci dalamtransisi meristem tunas jadi meristem bunga.Berbeda dengan sistem pada Arabidopsis,pembungaan kakao termasuk tipe cauliflorous,bunga dapat muncul dari bantalan bunga yangsama sepanjang tahun. Dalam penelitian inihomolog LFY diisolasi dari bantalan bunga aktifmenggunakan RT-PCR dengan sepasang primerspesifik yang dirancang berdasarkan sekuenDNA di kedua ujung gen tersebut. Pemeriksaangel elektroforesis menunjukkan adanya amplikontunggal berukuran 1,2 kb. Setelah dimurnikandari gel, amplikon dapat diklon ke dalam selkompeten E. coli galur XL1 Blue menggunakanvektor pGEM-T Easy dengan orientasi yangsesuai dengan promoter T7 dari vektor. AnalisisBLASTX sekuen DNA membuktikan bahwaamplikon tersebut adalah homolog dari genLEAFY. Analisis penjajaran dengan mengguna-kan ClustalW menunjukkan bahwa gen cTcLFYtersebut memiliki homologi yang tinggi dengangen sejenis dari tanaman keras lainnya sepertitanaman jeruk, anggur, apel dan poplar.Homologi tertinggi (daerah terkonservasi)terdapat pada ujung (terminal) C dari proteinyang disandinya.
Kloning gen LEAFY kakao dari jaringan bantalan bunga aktif Cloning of cacao LEAFY gene from the active flower cushions Tetty CHAIDAMSARI; Rita HAYATI; Auzar SYARIEF; Aswaldi ANWAR; Djoko SANTOSO
Menara Perkebunan Vol. 77 No. 2: 77 (2), 2009
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v77i2.179

Abstract

SummaryAttempts to improve productivity of cacaoplantations lead us to study the molecularmechanism of flowering. In the model speciesArabidopsis thaliana as well as some otherspecies, LEAFY is a central regulatory gene forthe transition of shoot apical meristems toflowering meristems. Different from that ofArabidopsis, cacao inflorescence is acauliflorous type, by which flowers can developrepeatedly from the same flower cushion on thetrunk. In this research, a LEAFY homolog wasisolated from active flower cushion with RT-PCRusing a pair of DNA primer specifically designedto isolate its complete cds. Gel electrophoresisexamination indicated the presence of a 1.2 kbamplicon. Purified from the gel, this DNAfragment was cloned into competent cells ofE. coli XL1 Blue using pGEM-T Easy cloningvector at an orientation according to the T7promoter of the plasmid. Sequence analysis usingBLASTX, showed that the amplicon was LEAFY(LFY) homolog. Alignment analysis using ClustalW indicated that the cTcLFY highly homologousto those from other perennial crops such ascitrus, grape, apple and poplar. The highesthomology (conserved region) was found in the Cterminal of the encoded proteins.RingkasanUsaha untuk meningkatkan produktivitasperkebunan kakao telah mendorong penelitianmolekuler tentang mekanisme pembungaankakao. Pada tanaman model Arabidopsis thalianadan lainnya, LEAFY merupakan gen kunci dalamtransisi meristem tunas jadi meristem bunga.Berbeda dengan sistem pada Arabidopsis,pembungaan kakao termasuk tipe cauliflorous,bunga dapat muncul dari bantalan bunga yangsama sepanjang tahun. Dalam penelitian inihomolog LFY diisolasi dari bantalan bunga aktifmenggunakan RT-PCR dengan sepasang primerspesifik yang dirancang berdasarkan sekuenDNA di kedua ujung gen tersebut. Pemeriksaangel elektroforesis menunjukkan adanya amplikontunggal berukuran 1,2 kb. Setelah dimurnikandari gel, amplikon dapat diklon ke dalam selkompeten E. coli galur XL1 Blue menggunakanvektor pGEM-T Easy dengan orientasi yangsesuai dengan promoter T7 dari vektor. AnalisisBLASTX sekuen DNA membuktikan bahwaamplikon tersebut adalah homolog dari genLEAFY. Analisis penjajaran dengan mengguna-kan ClustalW menunjukkan bahwa gen cTcLFYtersebut memiliki homologi yang tinggi dengangen sejenis dari tanaman keras lainnya sepertitanaman jeruk, anggur, apel dan poplar.Homologi tertinggi (daerah terkonservasi)terdapat pada ujung (terminal) C dari proteinyang disandinya.
Co-Authors . Adrinal . Giyanto Adhevin, Shadiq Octaryan Adib, Muhammad Afrima Sari Agus Sutanto Agus Zainul Arifin Agustian Agustian Alfi Asben Anwar Kasim Aprizal Zainal Armansyah Armansyah Armansyah Armansyah Armansyah Atman Atman Atman Atman Atzim Baitur Rahman Aulia Aryanti, Nissa Auzar SYARIEF Auzar Syarif A’yun, Qurrata Benni Satria Dede Suhendra Dewi, Riska Kurnia Dewi, Yulia Djoko Santoso Eka Susila elara resigia Endang Murwantini Fakhruzy Fakhruzy Fakhruzy, Fakhruzy Fri Maulina Giyanto . Gusviani, Nisa Azzahra Halimatus Syahdia Hasibuan Henny Puspita Sari Hervani, Dini Ilham Havifi Indra Dwipa Irawati Chaniago Irawati Irawati Irawati Irawati Irfan Suliansyah Itra, Novi Rahmat Kiki Yulianto KRISTINA, NILLA Mismawarni Srima Ningsih Mufti Gustrianda Muhammad Iqbal Abdi Lubis Muhammad Parikesit Wisnubroto Nalwida Rozen Netti Herawati nFN noflindawati nFN Yusniwati Nizar Hanafiah Nasution Noflindawati Noflindawati Noprizal Noprizal Nova Suryani Nugraha Ramadhan P.K. Dewi Hayati P.K. Dewi Hayati Pagil Prawinata Prasetyo, Rival Pratama, Rafdi Rahmi Azhari Ramadhani, Hanggraini Ramadhani, Lembayung Maghfira Raudha Thaib Rengga Septiadi Rita Hayati Rivandi, Oktavia Sanna Paija Hasibuan Sari, Afrima Sari, Lusi Puspika SATRIYAS ILYAS Satriyas Ilyas Silitonga, Yusnita Wahyuni Silvadriyanto, Reihan Siska Efendi Sri Heriza Sudarsono Sudarsono Sudarsono Suhendra, Dede Sumbari, Chika Susila, Rahma Syafrimen Yasin Tetty CHAIDAMSARI UJANG KHAIRUL Utami, Ami Sukma Wahyuni, Ony Tri Yanuar Yanuar Yulia Dewi Yusniwati Yusniwati Zahlul Ikhsan Zebua, Exel Valentino