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All Journal HAYATI Journal of Biosciences Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Buletin Agrohorti Jurnal Hortikultura Indonesia (JHI) Jurnal Agrista JURNAL PERBENIHAN TANAMAN HUTAN Indonesian Journal of Agricultural Science Jurnal Hortikultura Jurnal Pengkajian dan Pengembangan Teknologi Pertanian Jurnal Penelitian Tanaman Industri Buletin Penelitian Tanaman Rempah dan Obat Perspektif : Review Penelitian Tanaman Industri Pelita Perkebunan AGRIVITA, Journal of Agricultural Science Jurnal Agroekoteknologi SEMIRATA 2015 Jurnal Agro Jurnal Perlindungan Tanaman Indonesia JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Informatika Pertanian Jurnal Penelitian Pertanian Tanaman Pangan Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Jurnal Hexagro Jurnal Penelitian Tanaman Industri (Littri) Perspektif, Review Penelitian Tanaman Industri Buletin Penelitian Tanaman Rempah dan Obat Jurnal Agrotropika Berkala Penelitian Hayati Agrotech Journal
Satriyas Ilyas
Departemen Agronomi Dan Hortikultura, Fakultas Pertanian IPB Jl. Meranti Kampus IPB Darmaga, Bogor 16680
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology

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POTASSIUM FERTILIZER AND YOUNG SHOOT REMOVAL OF LARGE WHITE GINGER PLANT IMPROVE RHIZOME SEEDS STORABILITY Melati Melati; Satriyas Ilyas; Endah Retno Palupi; Anas D. Susila
Jurnal Penelitian Tanaman Industri Vol 26, No 2 (2020): December, 2020
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v26n2.2020.92-107

Abstract

The application of the appropriate potassium dosage is expected to produce high quality of rhizome seeds, hence improving its storability.Growing shoots are a strong sink. Thus, shoot removal aims to divert the photosynthate partition of shoots to the rhizome to improve rhizome development. The purpose of the research was to evaluate K dosage and young shoot removal:s effect to improve the production and quality of rhizome seeds of large white ginger. The experiment was arranged in the glasshouse in a split-plot design with four replications. The main plot was shoot removal treatments: 1) un removal, and 2) young shoots removal at six months after planting(MAP). The subplots were five doses of K; 0, 150 kg/ha, 300kg/ha, 450kg/ha, 600 kg/ha equivalent to 0, 3.75, 7.5, 11.25 and 15 g/plant respectively. The potassium fertilization was two times at 1 and 3 MAP, half dosage for each application. There was no interaction between young shoot removal and potassium dosage on the rhizome’s yield, physical, and biochemical quality. However, potassium dosage affected seed viability significantly. Potassium dosage presented a quadratic response with 7.5 g K/plant gave the best seed viability, whereas the optimum dosage for plant height was at 6.7 g K/plant. Shoot removal at 6 MAP produced seeds with enhanced storability, up to 9 months. Furthermore, it also improved seed viability as indicated by better seed growth than unremoval shoot treatment.Keywords: optimum dosage, seed quality, yield, Zingiber officinal. AbstrakPEMUPUKAN KALIUM DAN PEMBUANGAN TUNAS MUDA TANAMAN JAHE MENINGKATKAN DAYA SIMPAN BENIH JAHE PUTIH BESARPemberian pupuk K yang tepat diharapkan dapat menghasilkan benih rimpang yang bermutu tinggi karena dapat disimpan dalam jangka waktu yang panjang. Tunas yang sedang berkembang merupakan sink yang kuat. Pembuangan tunas bertujuan untuk dapat mengalihkan partisi fotosintat dari tunas ke rimpang. Percobaan ini bertujuan untuk mendapatkan dosis pupuk K dan mengetahui pengaruh pembuangan tunas muda terhadap produksi benih dan mutu rimpang jahe putih besar (JPB) sehingga memiliki daya simpan lama. Percobaan disusun dengan rancangan petak terbagi dengan empat ulangan. Petak utama adalah pembuangan tunas yaitu: 1)tanpa pembuangan tunas, dan 2)tunas muda dibuang pada 6 bulan setelah tanam (BST). Anak petak adalah lima dosis pupuk K yang diberikan pada 30 dan 90 BST yaitu: 1) tanpa K, 2) 150 kg/ha, 300 kg/ha, 450kg/ha, 600 kg/ha. Dosis pupuk tersebut setara dengan penambahan 0; 3,75; 7,5; 11,25 dan 15 g per tanaman masing-masing 1/2 dosis pada setiap pemberian. Hasil percobaan menunjukkan bahwa faktor tunggal pembuangan tunas muda pada umur 6 bulan setelah tanam (BST) dan pupuk K tidak berpengaruh terhadap produksi dan mutu fisik serta biokimia rimpang. Dosis pupuk K memengaruhi viabilitas benih yang dihasilkan. Viabilitas benih terbaik didapatkan pada penambahan pupuk K dosis 7,5 g/tanaman dengan respon kuadratik dan konsentrasi optimum untuk tinggi tanaman adalah 6,7 g/tanaman. Pembuangan tunas muda tanaman induk pada 6 BST menghasilkan benih yang dapat disimpan dalam jangka waktu 9 bulan serta viabilitas benih dengan pertumbuhan bibit yang lebih baik dibandingkan tanaman tanpa pembuangan tunas.Kata kunci: dosis optimum, mutu benih, produksi, Zingiber officinal.
MUTU FISIOLOGIS RIMPANG BENIH JAHE PUTIH BESAR SELAMA PENYIMPANAN DENGAN PELAPISAN LILIN DAN APLIKASI PACLOBUTRAZOL Devi Rusmin; MR Suhartanto; Satriyas Ilyas; Dyah Manohara; E Widajati
Buletin Penelitian Tanaman Rempah dan Obat Vol 26, No 1 (2015): Buletin Penelitian Tanaman Rempah dan Obat
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/bullittro.v26n1.2015.35-46

Abstract

Rimpang benih jahe putih besar tidak dapat disimpan lama karena mudah berkerut dan bertunas. Untuk meningkatkan daya simpan benih jahe telah dilaksanakan percobaan yang bertujuan untuk mengetahui pengaruh pelapisan lilin dengan aplikasi Paclobutrazol (PBZ) terhadap perubahan mutu fisiologis rimpang benih JPB selama penyimpanan. Percobaan dilaksanakan di kamar kaca dan Laboratorium Teknologi Benih, Balai Penelitian Tanaman Rempah dan Obat sejak Januari sampai September 2014. Percobaan disusun dalam rancangan petak terbagi dengan tiga ulangan. Sebagai petak utama adalah dua suhu ruang simpan (1) (26-280C) (RH 70-80%) dan (2) (20-220C) (RH 65-75%), sebagai anak petak adalah perlakuan benih (1) kontrol (tanpa bahan pelapis lilin); (2) pelapisan lilin + PBZ 0 ppm, (3) pelapisan lilin + PBZ 500 ppm; (4) pelapisan lilin + PBZ 1.000 ppm; dan (5) pelapisan lilin + PBZ 1.500 ppm. Hasil penelitian menunjukkan bahwa kombinasi pelapisan lilin dengan aplikasi PBZ sampai 1.500 ppm belum mampu menekan penyusutan bobot rimpang benih JPB sampai empat bulan setelah simpan, baik pada pada suhu penyimpanan 26-280C maupun pada suhu 20-220C. Kombinasi pelapisan lilin dengan aplikasi PBZ 1.500 ppm pada suhu 20-220C mampu menekan persentase rimpang bertunas sampai tiga bulan simpan dan menekan laju respirasi sampai empat bulan.
PENGARUH KONDISI SIMPAN DAN PERLAKUAN OSMOCONDITIONING TERHADAP VIABILITAS BENIH GMELINA (Gmelina arborea Roxb) Syamsuddin Syamsuddin; Endang Murniati; Satriyas Ilyas; Endah Retno Palupi
Jurnal Agrista Vol 7, No 3 (2003): Volume 7 Nomor 3 Desember 2003
Publisher : Fakultas Pertanian, Universitas Syiah Kuala

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Abstract

The research was conducted to study the effect of storage condition and osmoconditioning treatment on the viability of gmelina seed. The experiment was designed as a factorial experiment in a completely randomized design. The factors studied were storage condition (K), and osmoconditioning treatment (c). storage condition consist of room temperature condition (28-32oC) (K1) and under aircondition temperature (K2), where as osmoconditioning treatment consist of control (Co), PEG 0,4 MPa (C1), PEG 0,8 MPa (C2), KNO3 0,5 MPa (Ca3) and KNO3 0,1 MPa (C4). Observation was carried out in beweekly basis during sixteen weeks storage periods. The parameters observed were potential viability, growth strength vigour, and rate of metabolic activity changes. The research results showed the osmoconditioning treatment using PEG 0,4 and 0,8 MPa significantly increase total normal seedling. Moreover, osmoconditioning treatment using KNO3 0,5 MPa, and 1,0 MPa in the condition at under air conditioner room increase free fatty acid of the storage seed.
Efektifitas Daya Hambat Minyak Nabati Terhadap Pertumbuhan Koloni Beberapa Patogen Terbawa Benih Cabai Secara In Vitro Syamsudin Syamsudin; Satriyas Ilyas; Diah Manohara; Sudarsono Sudarsono
Jurnal Agrista Vol 11, No 2 (2007): Volume 11 Nomor 2 Agustus 2007
Publisher : Fakultas Pertanian, Universitas Syiah Kuala

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Abstract

Effectiveness of In Vitro Inhibitor Effects of Botanical Pesticides on Growth of Some Pepper Seed Born Pathogens ColoniesABSTRACT. Recently, the use of bio-pesticides significantly increases to control some plant diseases due to the drawback of chemical pesticides to environment. Some experiment have been done to evaluate the effectiveness of some vegetable oil as bio-pesticides to inhibit the colony growth of pepper seed born pathogens in vitro. Various concentrations of vegetable oil were added onto potato dextrose agar medium (PDA), afterward, the growth of pathogen colony in treatment was compared to control (medium without vegetable oil). Evaluation was done in certain period time depend on the growth of every pathogen colony Among the vegetable soil tested, clove oil and Andropogon nardus oil showed a very high inhibitory effect to colony growth of P. capsici, C. capsici, F. oxyporum, R solani, and S. rolfsii. Concentration 0.01% of those oil already inhibited the colony growth of all pathogens more than 75%, with the exception of A. nardus oil which required 0.03% to inhibit C. capsici colony. Concentration of clove and A. nardus oil above 0.01% completely inhibited growth of all pathogens colonies, except for F. oxyporum. Meanwhile, others vegetable oil like Curcuma xanthorrhiza, Kaempferia galangal, Kaempferia pandurata, and Curcuma domestica oil have a low inhibitory effect on growth of all pathogens colonies. To get inhibitory effect more than 75% on the growth of colonies, those vegetable oil needed concentration higher than 0.02%. However, K. galangal and C. domestica required 0.02% oil concentration for P. capsici and S. rolfsii, respectively.
Persistensi dan Pematahan Dormansi Benih pada Beberapa Varietas Padi Gogo Satriyas Ilyas; Wan Tin Diarni
Jurnal Agrista Vol 11, No 2 (2007): Volume 11 Nomor 2 Agustus 2007
Publisher : Fakultas Pertanian, Universitas Syiah Kuala

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Abstract

Persistence and Breaking Seed Dormancy of Land Rice CultivarsABSTRACT. Previous studies have been done to break seed dormancy in rice (Oryza sativa L.). However, since the behavior of seed dormancy are different for different varieties, there is a need to find effective methods for breaking dormancy in four most important cultivars of low land rice. Low land rice seeds were harvested at 95 days after planting (cv. Kalimutu and cv. Gajah Mungkur), 105 dap (cv. Way Rarem) or 115 dap (cv. Jatiluhur), and dried to 13% moisture content. The seeds were stored in an envelope paper at ambient temperature and relative humidity (28-310C, RH 81-89%) for up to 9 weeks with 1 week interval. Persistency of seed dormancy in each cultivar was determined based on the time needed to reach a minimum of 85% germination capacity. Various methods of breaking dormancy were applied: untreated control, soaking in water for 24 h, soaking in water for 48 h, soaking in 1% KN03 for 24 h, soaking in 1% KN03 for 48 h, soaking in 120 ppm GA3 for 24 h, soaking in 120 ppm GA3 for 48 h, heating 500C for 72 h, heating 500C for 48 h + heating in water for 24 h, heating 500C for 48 h + heating in water for 48 h, heating 500C for 48 h + heating in 1% KN03 for 24 h, heating 500C for 48 h + heating in 1% KN03 for 24 h, soaking in water for 24 h + in damp cloth for 24 h, mantriconditioning using sawdust and mantriconditioning using rice hull ash. Soon after the treatment the seeds were germinated, and seed viability and vigor were observed. Results of experiment showed the viability and vigor of low land rice seeds were increased after storage. Persistence of seed dormancy was different in each cultivar i.e. 3 weeks in Kalimutu (germination capacity 90%), 4 weeks in Way Rarem (87%), 6 weeks in Gajah Mungkur (92%), and 9 weeks in Jatiluhur (85%). Soaking the seeds in 1% KN03 for 48 h was the most effective method for breaking dormancy of Kalimutu, Gajah Mungkur and Way Rarem at harvest (0 weeks). Heating the seeds in a 500C oven for 48 h followed by soaking in water 24 h was the only effective method to break dormancy in cv. Jatiluhur at 2 weeks after harvest, while the time of harvest none of the method was effective.
DIGITAL IMAGE ANALYSIS USING FLATBED SCANNING SYSTEM FOR PURITY TESTING OF RICE SEED AND CONFIRMATION BY GROW OUT TEST Mira Landep Widiastuti; Aris Hairmansis; Endah Retno Palupi; Satriyas Ilyas
Indonesian Journal of Agricultural Science Vol 19, No 2 (2018): December 2018
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v19n2.2018.p49-56

Abstract

The common method used for purity testing of rice seed is human visual observation. This method, however, has a high degree of subjectivity when dealing with different rice varieties which have similar morphology. Digital image analysis with flatbed scanning for purity testing of rice seed was proposed by investigating the morphology of rice seeds and confirmation by grow out test (GOT) in the field. Two extra-long seed varieties were used in this study including a red rice Aek Sibundong and an aromatic rice Sintanur. The identification on 14 parameters of morphological characteristics indicated that only six parameters were correlated, i.e. area, feret, minimum feret, aspect ratio, round, and solidity. The purity of rice seed can be effectively determined using digital image analysis of spikelet color and shape. Based on the discriminant analysis of the digital image the recognition rate of rice seed purity was higher than 99.2% for shape and 93.55% for color. The method, therefore, has a potential to be used as a complement in rice seed purity testing to increase the accuracy of human visual method and it is more sensitive than GOT.
Efektivitas Gen CP PSTV dalam Memproteksi Nicotiana benthamiana Transgenik TO terhadap Serangan Peanut Stripe Virus Sholeh Avivi; Sudarsono Sudarsono; Satriyas Ilyas; Hajrial Aswidinnoor
Jurnal Perlindungan Tanaman Indonesia Vol 11, No 1 (2005)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12103

Abstract

The aims of this research were: (1) to obtain the transgenic Nicotiana benthamiana T0 which have various cp Peanut stripe virus (PStV) genes construct (pBINRCP 1, pBINRCP2, pBINRCP3, and pBINRCP4); (2) to investigate the resistance of transgenic N. benthamianaT0 toward PStV infection; (3) to investigate the effectiveness of those construct to protect N. benthamiana T0 toward PStV infection. To achieve those objectives N. benthamiana T0 (70 plants) were regenerated, PCR tested and infected with PStV using biological analysis methods. The result showed that all of PStV construct gave the resistancy toward PStV infection. The phenotypic respon of those plants were the PBINRCPl plants 35.7% resistance, 0% recovery, and 64.3% susceptible, the PBINRCP2 plants 41.7%, resistance, 0% recovery, and 58,3% susceptible, the PBINRCP3 plants 71.4% resistance, 0% recovery, and 28.6% susceptible, and the PBINRCP4 plants 25.0% resistance, 25.0% recovery, and 50.0% susceptible.
Deteksi Bakteri Clavibacter michiganensis subsp. michiganensis pada Benih Tomat Komersial yang Beredar di Indonesia Aswaldi Anwar; Satriyas Ilyas; Sudarsono Sudarsono
Jurnal Perlindungan Tanaman Indonesia Vol 10, No 2 (2004)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12118

Abstract

The existence of Clavibacter michiganensis subsp. michiganensis (Cmm), the pathogen causing bacterial canker, on 22 lots of tomato seeds distributed commercially in Indonesia was evaluated. Isolation of suspected Cmm colonies were conducted by agar dilution plating on semiselective SCM medium followed by confirmation of colony identity on YDC medium. Identity of suspected Cmm colony was confirmed using IF test, pathogenicity test on tomato seedlings, hypersensitivity test on leaf of Mirabilis jalapa and Nicotiana tabacum, ELISA, and Cmm specific DNA amplification by PCR. After seed extraction and evaluation of the extract on semi-selective SCM medium and confirmastion by IF test, at least six tomato seed lots were contaminated with Cmm. After more confirmation using pathogenicity and hypersensitivity test, ELISA, and PCR amplification, at least three seed lots were confirmed possitively to carry Cmm.
PENGARUH LAMA PENYIMPANAN DAN PERLAKUAN BENIH TERHADAP PENINGKATAN VIGOR BENIH KAKAO HIBRIDA Baharudin ;; Satriyas Ilyas; Mohamad Rahmad Suhartanto; Agus Purwantara
Jurnal Pengkajian dan Pengembangan Teknologi Pertanian Vol 13, No 1 (2010): Maret 2010
Publisher : Balai Besar Pengkajian dan Pengembangan Teknologi Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jpptp.v13n1.2010.p%p

Abstract

Effect of Length Storage and Seed Treatment to Improve Seed Vigour of Kakao Hybrid. Cacaoseeds are categorized as recalcitrant which have some problems such as: hight water content, short storability,sensitive to desiccation, sensitive to low temperature and pathogen contamination. The aims of the research wasto observe the interaction effect between the period of storage and seed treatment on viability and vigor of hybridcacao seeds and seedling of TSH 858. This research was conducted at Seed Main Garden of Indonesian Centreof Coffee and Cacao Research Institute (Puslitkoka) in Jember, Laboratory of Bogor Agricultural University, andMicrobiological laboratory and glass house of Biotechnology Research Institute for Estate Crops of Indonesia inBogor during May to December 2008. Seeds used were derived from results of open cross pollination betweencacao TSH 858 vs Sca 6 from Puslitkoka. Factorial completely randomized design was used, the first factor wasthe period of seed storage and the second factor was the seed treatment. Result showed that interaction betweenthe period of seed storage and seed treatment were statistically significant on germination ability, speed growthrelatively, T50, and number of leaf. The germination ability of seed decreased after 4 weeks storage, but the useof Trichoderma harzianum DT/38 and T. pseudokoningii DT/39 able to increase the germination ability from 8%to 63%. Seed vigor was showed by speed growth relatively, growth velocity (T50- ), and number of leaf werealso improved in matriconditioned seeds compared with the untreated ones. Matriconditioning plus T. harzianumDT/38 and T. pseudokoningii DT/39 treatment also increased index of vigor 32%, speed of germination 0, 5 mg,height of seedlings 3,5 cm, length of roots 0,6 cm and number of roots 8,7 compared with those were untreated.Key words : Biological control, hybrid seed, seed storages, seed vigor, Theobroma cacaoBenih kakao tergolong rekalsitran yang memiliki beberapa kendala antara lain berkadar air tinggi, periodehidup yang relatif singkat, tidak tahan desikasi dan suhu rendah, dan mudah terkontaminasi patogen. Penelitianbertujuan untuk mengetahui pengaruh interaksi antara lama penyimpanan dengan perlakuan benih terhadapviabilitas dan vigor benih, serta bibit kakao hibrida TSH 858. Penelitian dilaksanakan di Kebun Induk Benih PusatPenelitian Kopi dan Kakao Indonesia Jember, Laboratorium Benih IPB, Laboratorium dan rumah kaca mikrobiologiBalai Penelitian Bioteknologi Perkebunan Indonesia Bogor, pada bulan Mei sampai Desember 2008. Benih hibridaberasal dari hasil persilangan terbuka antara kakao TSH 858 x Sca 6 dari Puslitkoka. Penelitian menggunakanPengaruh Lama Penyimpanan dan Perlakuan Benih terhadap Peningkatan Vigor Benih Kakao Hibrida (Baharudin, SatriyasIlyas, Mohamad Rahmad Suhartanto dan Agus Purwantara)74rancangan lingkungan acak lengkap faktorial, faktor pertama adalah lama penyimpanan secara alami dan faktorkedua perlakuan benih. Hasil penelitian menunjukkan interaksi antara lama penyimpanan benih dengan perlakuanbenih nyata mempengaruhi daya berkecambah, kecepatan tumbuh relatif,T50dan jumlah daun. Benih kakaosetelah penyimpanan empat minggu menunjukkan daya berkecambah yang menurun, tetapi dengan perlakuanmatriconditioning plus Trichoderma harzianum DT/38 dan T. pseudokoningii DT/39 mampu meningkatkan dayakecambah dari 8% menjadi 63%. Vigor benih yang ditunjukkan oleh kecepatan tumbuh relatif, kecepatan tumbuh(T50 -), dan jumlah daun juga ikut meningkat dengan perlakuan matriconditioning. Perlakuan matriconditioningplus T. harzianum DT/38 dan T. pseudokoningii DT/39 mampu meningkatkan indeks vigor 32%, laju pertumbuhankecambah 0, 5 mg, tinggi bibit 3,5 cm, panjang akar 0,6 cm dan jumlah akar 8,7 dibanding tanpa perlakuan.Kata kunci : Pengendalian hayati, benih hibrida, penyimpanan benih, Theobroma cacao, vigor benih
ISOLASI DAN IDENTIFIKASI CENDAWAN TERBAWA BENIH KAKAO HIBRIDA BAHARUDIN BAHARUDIN; AGUS PURWANTARA; SATRIYAS ILYAS; MOHAMAD RAHMAD SUHARTANTO
Jurnal Penelitian Tanaman Industri Vol 18, No 1 (2012): Maret 2012
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v18n1.2012.40-46

Abstract

ABSTRAKBenih merupakan komponen dasar dalam menentukan produktivitastanaman kakao. Benih yang sehat dapat merupakan faktor penting dalammenentukan keberhasilan produktivitas kakao. Benih kakao mempunyaikadar air cukup tinggi sehingga berpotensi terinfeksi cendawan, yangdapat menurunkan mutu benih dan produksi kakao. Penelitian bertujuanuntuk mengisolasi dan mengidentifikasi beberapa cendawan terbawa benihpada kakao hibrida. Penelitian dilakukan di Kebun Induk Benih PusatPenelitian Kopi dan Kakao Indonesia, Jember, Laboratorium Mikro-biologi, Balai Penelitian Bioteknologi Perkebunan Indonesia, danLaboratorium Pengendalian Hayati IPB Bogor pada bulan Juni sampaiOktober 2008. Penelitian menggunakan benih kakao hibrida dari hasilpersilangan buatan antar TSH 858 dengan Sca 6, dan percobaan disusundengan rancangan acak lengkap dengan 3 ulangan. Benih ditumbuhkanpada 3 media, yaitu water agar (WA), potato dextrose agar (PDA), dankertas saring (KS). Tingkat infeksi pada benih diamati setiap hari dandianalisis dengan analisis sidik ragam dan dilanjutkan dengan uji selangberganda Duncan. Cendawan diisolasi, dibiakkan, dimurnikan, dandiidentifikasi dengan menggunakan buku kunci identifikasi. Tingkatinfeksi cendawan terbawa benih kakao hibrida tertinggi terdapat pada harikeempat (35,00%) dan kelima (51,67%) pada media PDA. Sebanyak 13spesies cendawan terbawa benih kakao hibrida berhasil diidentifikasidengan menggunakan media WA dan PDA, serta 8 spesies cendawandengan media KS. Ke-13 cendawan terbawa benih yang ditemukan sangatberpotensi menurunkan mutu fisiologis benih dan produktivitas kakao.Cendawan tersebut perlu diuji lebih lanjut karena masing-masing memilikisifat-sifat patogenik, saprofitik, atau antagonistis terhadap cendawan lainpada benih kakao. Cendawan terbawa benih kakao hibrida paling dominanadalah Aspergillus spp., Penicillium chrysogenium, Coletotrichumacutatum, Curvularia geniculata, dan Fusarium spp. Cendawan-cendawanyang diduga berbahaya adalah Aspergillus spp., Coletotrichum acutatum,Curvularia  geniculata,  Fusarium  spp.,  Phoma  glomerata,  danMacrophoma sp., dan yang diduga bersifat patogenik adalah Aspergillusflavus, Aspergillus ochraceus, Cladosporium herbanum, Curvulariageniculata, Fusarium oxysporum, Phoma glomerata, dan Macrophoma sp.Kata kunci : Theobroma cacao, benih hibrida, infeksi cendawan, mediatanamIsolation and Identification of Fungi on Hybrid Cacao SeedsABSTRACTSeed is the basic component influencing the productivity of cacaoplantation. Healthy seed is the most important factor in determining thesuccess of cacao productivity. Moisture content of cacao seeds is quitehigh potentially to cause fungi infection, which can further reduce seedquality and cacao production. The research aimed at isolating andidentifying several seedborne fungi on hybrid cacao. The study wasconducted at main nursery of Indonesian Coffee and Cocoa ResearchInstitute Jember, Laboratory of Microbiology, Indonesian BiotechnologyResearch Institute for Estate Crops, and the Laboratory for BiologicalControl of IPB Bogor from June to October 2008. Research used hybridcacao seeds derived from crossing between TSH 858 x SCA 6, and theexperiment was arranged using completely randomized design with threereplicates. Cacao seeds were grown on three media, i.e. water agar (WA),potato dextrose agar (PDA), and filter paper (KS). Infection rates on theseedlings were observed every day and analyzed using ANOVA followedby Duncan's multiple regression test (DMRT). Fungi were isolated,cultured, purified, and identified using the identification keys. The highestrate of seedborn fungal infection occured on fourth (35.00%) and fifth(51.67%) days on PDA media. A total of 13 species of seedborn fungi onhybrid cocoa were identified by using WA and PDA media, as well as 8other species by using KS. The 13 seedborne fungi potentially reduce seedphysiological quality and cacao productivity. These fungi need to befurther tested because each has its own pathogenic, saprophytic, orantagonistic properties towards other fungi on cacao seeds. Predominantseedborn fungi on hybrid cacao were Aspergillus spp., Penicilliumchrysogenium, Coletotrichum acutatum, Curvularia geniculata, andFusarium spp. The fungi suspected harmful were Aspergillus spp.,Coletotrichum acutatum, Curvularia geniculata, Fusarium spp., Phomaglomerata, and Macrophoma sp., and those suspected pathogenic wereAspergillus flavus, Aspergillus ochraceus, Cladosporium herbanum,Curvularia geniculata, Fusarium oxysporum, Phoma glomerata, andMacrophoma sp.Key words : Theobroma cacao, fungi infection, hybrid seed, growingmedia
Co-Authors , Agustiansyah . Agustiansyah ., Syamsuddin Abdul Munif Abubakar Ibrahim Agus Purwantara Agus Purwantara AGUS PURWANTARA Agus Purwantara Agustiansyah Agustiansyah Ahmad Zamzami Ainun Nur Maulidina Hikmawati Aminuyati ANAS D SUSILA Anas D. Susila Anwar, Aswaldi Aprizal Zainal Aris Hairmansis Atin Yulyatin Aulia Zakia Bagus Kukuh Udiarto Baharudin Baharudin ; BAHARUDIN BAHARUDIN Belladina Farhana Budhianto, Bambang Candra Budiman Dedy Suhendy DEVI RUSMIN Devi Rusmin DEVI RUSMIN Diah Manohara Dianawati, Meksy Dyah - Manohara DYAH MANOHARA Dyah Manohara Dyah Manohara Dyah Manohara Dyah Manohara E Widajati Edi Guhardja Edy Suprianto Endah Retno Palupi Endang Murniati Endang Pudjihartati Enni Suwarsi Rahayu Eny Widajati Faiza C Suwarno Faiza C Suwarno Farih Najah Rosadiah Giyanto . Giyanto, Giyanto HAJRIAL ASWIDINNOOR Hamami, Happy Suryati Hipi, Awaludin Ikrarwati, Ikrarwati IRENG DARWATI Kartika Kartika Lalu Firman Budiman M.R. SUHARTANTO M.R. SUHARTANTO, M.R. Manohara, Dyah - Maryati Sari MELATI MELATI Memen Surahman Mira Landep Widiastuti Mohamad Arif Mohamad Rahmad Suhartanto MR Suhartanto Muhammad Machmud Muhammad Machmud Muhammad Machmud Nelly Fridayanti Qadir, Abdul Rubiyo Rubiyo RUSMIN, DEVI Sholeh Avivi Sri Wilarso Budi Sudarsono Sudarsono . Sudarsono Sudarsono Sudarsono Sudarsono Sudarsono Sudarsono Sudarsono Sudarsono Susila, A D Syamsuddin Syamsuddin Syamsudin Syamsudin Trikoesoemaningtyas Trikoesoemaningtyas Wan Tin Diarni Wattimena, G A Yukti, AmiyarsI Mustika