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MUTU FISIOLOGIS RIMPANG BENIH JAHE PUTIH BESAR SELAMA PENYIMPANAN DENGAN PELAPISAN LILIN DAN APLIKASI PACLOBUTRAZOL Devi Rusmin; MR Suhartanto; Satriyas Ilyas; Dyah Manohara; E Widajati
Buletin Penelitian Tanaman Rempah dan Obat Vol 26, No 1 (2015): Buletin Penelitian Tanaman Rempah dan Obat
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/bullittro.v26n1.2015.35-46

Abstract

Rimpang benih jahe putih besar tidak dapat disimpan lama karena mudah berkerut dan bertunas. Untuk meningkatkan daya simpan benih jahe telah dilaksanakan percobaan yang bertujuan untuk mengetahui pengaruh pelapisan lilin dengan aplikasi Paclobutrazol (PBZ) terhadap perubahan mutu fisiologis rimpang benih JPB selama penyimpanan. Percobaan dilaksanakan di kamar kaca dan Laboratorium Teknologi Benih, Balai Penelitian Tanaman Rempah dan Obat sejak Januari sampai September 2014. Percobaan disusun dalam rancangan petak terbagi dengan tiga ulangan. Sebagai petak utama adalah dua suhu ruang simpan (1) (26-280C) (RH 70-80%) dan (2) (20-220C) (RH 65-75%), sebagai anak petak adalah perlakuan benih (1) kontrol (tanpa bahan pelapis lilin); (2) pelapisan lilin + PBZ 0 ppm, (3) pelapisan lilin + PBZ 500 ppm; (4) pelapisan lilin + PBZ 1.000 ppm; dan (5) pelapisan lilin + PBZ 1.500 ppm. Hasil penelitian menunjukkan bahwa kombinasi pelapisan lilin dengan aplikasi PBZ sampai 1.500 ppm belum mampu menekan penyusutan bobot rimpang benih JPB sampai empat bulan setelah simpan, baik pada pada suhu penyimpanan 26-280C maupun pada suhu 20-220C. Kombinasi pelapisan lilin dengan aplikasi PBZ 1.500 ppm pada suhu 20-220C mampu menekan persentase rimpang bertunas sampai tiga bulan simpan dan menekan laju respirasi sampai empat bulan.
POLIETILENA GLIKOL (PEG) DALAM MEDIA IN VITRO MENYEBABKAN KONDISI CEKAMAN YANG MENGHAMBAT TUNAS KACANG TANAH (Arachis hypogaea L.) Enni Suwarsi Rahayu; Edi Guhardja; Satriyas Ilyas; Sudarsono
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 11 No 1 (2005): December 2005
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/457

Abstract

The objectives of this experiment were to determine response of epycotyl of nine peanut cultivars against polyethylene glycol (PEG 6000) induced stress under in vitro conditions, effective concentration of PEG to inhibit growth and development of epycotyl, evaluate tolerance of the cultivars against PEG stress, and evaluate changes in total proline content due to PEG stress. Growing epycotyls from peanut seeds (TDK) or from embryo axis (TTK) were planted on liquid MS-0 medium containing PEG 6000 (0%, 5%, 10%, 15%, and 20%). Growth, development, and the tissue damage score of the epycotyl were observed after six weeks. Total content of proline were analyzed for stressed and non stressed epycotyl to determine effect of PEG stress on proline accumulation. Results of the experiment indicated that addition of PEG 6000 in to MS-0 medium inhibited growth and development of peanut epycotyls and increased its total proline content. Addition of PEG 6000 might be used to simulate drought stress under in vitro condition. PEG at 15% concentration was effective for inhibiting growth and development of epycotyl explant. The response of peanut epycotyls against medium containing 15% PEG 6000 might be used as alternative methods for screening peanut tolerance against drought stress. The TDK and TTK might be used as explant, while increased in shoot length (TTK), in leaf number (TDK and TTK), in milted leaf number (TDK and TTK), in root number (TDK) and score of tissue damage (TDK) might be used as criteria for drought tolerance.
PENGARUH PERLAKUAN BENIH DENGAN AGENS HAYATI DAN PEMUPUKAN TERHADAP PERTUMBUHAN TANAMAN, PRODUKSI DAN MUTU BENIH PADI DI LAPANG Agustiansyah Agustiansyah; Satriyas Ilyas; Sudarsono Sudarsono; Muhammad Machmud
JURNAL AGROTROPIKA Vol 17, No 2 (2012): Agrotropika Vol.17 No.2 2012
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (426.57 KB) | DOI: 10.23960/ja.v17i2.4284

Abstract

Biological agents is believed to function as fitostimulators, biofertilizers, and biopesticides. These capabilities have been widely reported by researchers on various crops. Application these agents to rice plants had not been widely reported, particu-larly in Indonesia. The purpose of this study was to determine the effect of seed treatment with rhizobacteria and use of fertilizer P on plant growth, seed quality in the field. The study experiments that were conducted in Research Station of Indonesian Rice Research Institue, Sukamandi, West Java. The experimental results show that P fertilizer application at the rate of 50 kg ha-1 produced plant height, number of tillers, number of pithy grains, and total number of grains per panicle better than that 100 kg ha-1.. Seed treatments by immersion in a mixture of P. diminuta A6 + B. subtilis 5/B) with or without matriconditioning increased plant height and num-ber of tillers. Seed treatment with matriconditioning + biological agents increased the number of grains and total number of grains. All the seed treatments and P fertilizer applications no significantly affected the physi-ological quality of seed yield, but reduced population of Xanthomonas oryzae pv.oryzae (Xoo) in the seeds. Keywords: bactericide, biofertilizer, biopesticide, seed patologis quality
PENGARUH PERLAKUAN BENIH PADI (Oryza sativa L.) YANG TERINFEKSI Xanthomonas oryzae PV. Oryze TERHADAP PERTUMBUHAN TANAMAN DAN HASIL DI RUMAH KACA Candra Budiman; Satriyas Ilyas
Hexagro Journal Vol. 2 No. 1 (2018)
Publisher : Universitas Perjuangan Tasikmalaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36423/hexagro.v2i1.117

Abstract

Penggunaan benih bermutu dari varietas unggul merupakan faktor utama dalampeningkatan produksi tanaman di lapangan. Penelitian bertujuan untuk menemukanperlakuan benih yang dapat meningkatkan mutu pertumbuhan dan produksi padi dirumah kaca. Percobaan menggunakan Rancangan Petak Terbagi dengan petak utama,yaitu varietas (IR64 dan Ciherang), dan anak petak perlakuan benih : kontrol, bakterisidasintetik (Agrept 0.2% b/v), minyak serai wangi (1% v/v), agens hayati (Pseudomonasdimunuta skala 4 Mc Farland), matriconditioning + bakterisida sintetik (streptomisin sulfat0.2% b/v), matriconditioning + minyak serai wangi (1% v/v), matriconditioning + agenshayati (Pseudomonas dimunuta skala IV Mc Farland). Dari penelitian ini didapatkan bahwapengaruh perlakuan benih meningkatkan petumbuhan tanaman pada fase awalpertumbuhan. Pada fase lebih lanjut, pengaruh varietas lebih berpengaruh. Varietasciherang secara umum lebih produktif dan tahan terhadap HDB dibanding varietas IR64.Perlakuan matriconditioning + streptomisin sulfat 0.2% dan matriconditioning P. dimunutameningkatkan daya berkecambah, tinggi tanaman dan jumlah anakan.Kata kunci : Ciherang, IR6, matriconditioning, perlakuan benih
Seleksi In Vitro Embrio Somatik Kacang Tanah pada Medium dengan Polietilen Glikol untuk Simulasi Kondisi Cekaman Kekeringan Enni Suwarsi Rahayu; Satriyas Ilyas; Sudarsono Sudarsono
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 23, No 1 (2006)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2006.23.1.141

Abstract

This study was aimed to evaluate the effectiveness of in vitro selection for identifying PEG insensitive somaclonal variant of peanut somatic embryos (SE). In one of the experiments,  evaluation on responses of four peanut cultivars against selective medium containing polyethylene glycol 6000 (PEG 6000) was conducted and sub-lethal concentration of PEG was determined. In the other experiment, in vitro selection on selective medium containing sub-lethal level of PEG was conducted to identify PEG insensitive SE of peanut. Secondary SE of peanut cv. Badak, Kelinci, Singa, and Zebra were cultured on liquid MS medium supplemented with 16 µM of picloram and 5%, 10%, 15%, or 20% of PEG 6000. Survival of explant, average number of proliferated SE/explant, and total number of proliferated SE after in vitro selection were recorded monthly, up to three months. Sub-lethal level of PEG was defined as one inhibiting more than 95% of the total number of proliferated SE. In vitro selection on medium supplemented with sub-lethal level of PEG 6000 was conducted on at least 4000-5000 SE of peanut cv. Kelinci and Singa. The PEG insensitive SE was identified after subsequent three months of in vitro selection. The results showed that supplementation of PEG 6000 on medium for induction of SE inhibited proliferation of peanut SE. Sub-lethal level was obtained at 15% concentration of PEG 6000. The frequencies of obtaining PEG insensitive SE of peanut cv. Kelinci was 8% to 10%  and for peanut cv. Singa was 10% to 12%. The R0 plants of peanut cv. Kelinci (62 R0 plants) and Singa (48 R0 plants) regenerated from PEG insensitive SE were obtained and grown in the glasshouse to produce R1 and R2 seeds.
STRUKTUR DAN METODE PERKECAMBAHAN BENIH ROTAN JERNANG (Daemonorops dransfieldii Rustiami) Eny Widajati; Nelly Fridayanti; Endah Retno Palupi; Satriyas Ilyas; Sri Wilarso Budi
Jurnal Perbenihan Tanaman Hutan Vol 10, No 1 (2022): Jurnal Perbenihan Tanaman Hutan
Publisher : Forest Tree Seed Technology Research & Development Center (FTSTRDC)/ Balai Penelitian dan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/bptpth.2022.10.1.81-99

Abstract

Buah rotan jernang merupakan hasil hutan bukan kayu bernilai ekonomi tinggi karena pada eksokarp buah terdapat resin. Buah rotan jernang dipanen sebelum benih mencapai masak fisiologis untuk mendapatkan resin kualitas tinggi sehingga tanaman tidak dapat meregenerasi secara alamiah dan rotan jernang di habitat alami semakin berkurang. Kendala dalam ketersedian benih bermutu adalah perkecambahan lama dan pertumbuhan bibit tidak seragam. Tujuan penelitian adalah untuk mendapatkan metode perkecambahan yang lebih cepat dan seragam, mempelajari proses perkecambahan, dan mengembangkan metode uji daya berkecambah. Percobaan pertama menggunakan RAL dua faktor. Faktor pertama adalah pencongkelan operkulum, terdiri atas dua taraf yaitu benih utuh dan benih tanpa operkulum. Faktor kedua adalah perendaman benih terdiri atas tiga taraf yaitu kontrol, perendaman menggunakan aquades dan KNO3 0,2%. Percobaan kedua menggunakan RAL satu faktor yaitu media perkecambahan terdiri atas media pasir dan cocopeat. Data dianalisis menggunakan SAS 9.4 dan diuji lanjut menggunakan DMRT pada taraf kepercayaan 5%. Pencongkelan operkulum dan tanpa perendaman dapat meningkatkan indeks vigor dan kecepatan tumbuh. Proses perkecambahan benih rotan jernang (benih tanpa operkulum) terdiri atas empat tahap yaitu terbentuk tangkai kotiledon, kotiledon ligule, akar dan daun. Hitungan pertama dan hitungan terakhir uji daya berkecambah adalah 72 dan 104 hari setelah tanam. Kriteria kecambah normal adalah panjang plumula minimum ±15 mm, akar berkembang dengan sempurna yaitu terdapat akar primer dan akar sekunder.  Media terbaik untuk perkecambahan benih rotan jernang adalah pasir. 
Optimasi Produksi dan Mutu Benih Padi Varietas PBM UBB 1 dengan Bakteri Pelarut Fosfat dan Pupuk Fosfat: Optimasi Produksi dan Mutu Benih Padi Varietas PBM UBB 1 dengan Bakteri Pelarut Fosfat dan Pupuk Fosfat Kartika Kartika; M. Rahmad Suhartanto; Abdul Munif; Endah Retno Palupi; Satriyas Ilyas
AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Vol 6 No 2 (2022): AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian
Publisher : Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (327.792 KB) | DOI: 10.33019/agrosainstek.v6i2.402

Abstract

One of the problems for phosphate fertilizing in ultisol soil is Al and Fe bounding and could be overcome by phosphate solubilizing bacteria activity.This study aimed to obtain the best dose of phosphate fertilizer with phosphate solubilizing bacteria for the production of upland rice seeds of the UBB1 PBM variety (Bangka Belitung University red rice 1) and its effect on seed quality. The research was carried out at the Research and Experimental Gardens – Faculty of Agriculture, Fisheries and Biology, University of Bangka Belitung, from August to December 2020. The design used was a split plot design, the main plot was phosphate solubilizing bacteria (treatment and control), and the subplots were fertilizers. phosphate (P1– quarter dose, P2– half dose, P2–three-quarter dose, P4– full dose). Seeds produced in the field were tested in the laboratory for viability and vigor. In the field, phosphate solubilizing bacteria (Burkholderia sp) can replace half the dose of phosphate fertilizer. This was indicated by the seed weight of plant-1 which did not differ between full dose + control and half dose + treatment. This fact was also identified in the viability and vigor of the seeds in laboratory testing. There was no significant difference in germination rate and vigor index between the two treatments. This finding shows that phosphate solubilizing bacteria can be applied for efficiency of phosphate fertilization and guaranteeing seed production and seed quality
Fine Bubbles Utilization to Increase Germination Rate of Oil Palm (Elaeis guineensis L. Jacq) Seeds Mohamad Arif; Satriyas Ilyas; Eny Widajati; Abdul Qadir; Edy Suprianto; Endah Retno Palupi
AGRIVITA, Journal of Agricultural Science Vol 45, No 1 (2023)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v45i1.3908

Abstract

The current method to break oil palm seed dormancy is the dry heat method which requires approximately 90 days for seeds to germinate with an average germination percentage. Therefore, hastening the process with a higher ratio of germinated seeds becomes necessary. This experiment aims to hasten and increase germination percentage by applying fine bubble water during the dormancy-breaking process. A seed lot derived from one bunch as replication is divided into two sets where fiber plugs from one of the sets are removed while the other set remains intact. Each set was re-divided into six experimental units soaked with tap water or fine bubble water for 1 to 3 days. The result showed that applying fine bubble water provided a better germination rate than tap water treatments. However, the total germination percentage between all treatments does not differ significantly. The germination peak of oil palm seed in the fine bubble water treatments occurred on the 26th day, 8 days faster than in the tap water treatments. The occurrence showed the potential use of fine bubble water application during dormancy breaking using the dry heat method.
EFFECT OF THE TREATMENT OF RICE SEED INFECTED BY XANTHOMONAS ORYZAE PV. ORYZAE ON GROWTH AND YIELD OF RICE IN THE FIELD Ahmad Zamzami; Satriyas Ilyas
Agrotech Journal Vol 2, No 2 (2017): Agrotech Journal (ATJ)
Publisher : Universitas Sembilanbelas November Kolaka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31327/atj.v2i2.317

Abstract

This research aimed to study the effect of treatment of rice seed infected with X. oryzae pv. Oryzae naturally to control bacterial leaf blight and to increase growth and yield of rice in the field. The research used Slit plot design with the main plot of variety consisting of IR64 and Ciherang, while the subplot is a seed treatment consisting of control, bactericide 0.2%  (Agrept 20WP), 1% citronella oil, biological agent Pseudomonas diminuta (McFarland IV scale ), matriconditioning + Agrept 0.2%, matriconditioning + 1% citronella oil, and matriconditioning + P. diminuta. Although seed treatment has not been able to control the bacterial leaf blight, it can increase the growth of seed and yield. Treatment of matriconditioning + Agrept 0.2% can increase the seed viability and dry weight of seedlings. Height of seedlings can be increased by the treatment of citronella oil, biological agents P. diminuta, matriconditioning + agrept 0.2%, and  matriconditioning + 1% citronella oil. Treatment of citronella oil, matriconditioning + P. diminuta, biological agents P. diminuta, and matriconditioning + Agrept 0.2% can increase the  estimated yield of ubinan/CCE harvest
Cacao (Theobroma cacao L.) Resistance Evaluation Againts Black Pod Diseaseand Effectiveness of Inoculation Methods Rubiyo Rubiyo; Agus Purwantara; Dedy Suhendy; Trikoesoemaningtyas Trikoesoemaningtyas; Satriyas Ilyas; Sudarsono Sudarsono
Pelita Perkebunan (a Coffee and Cocoa Research Journal) Vol 24 No 2 (2008)
Publisher : Indonesian Coffee and Cocoa Research Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iccri.jur.pelitaperkebunan.v24i2.101

Abstract

The general objective of this experiment was to standardize method of resistance evaluation of cacao germplasm against Phytophthora palmivora, the pathogen causing black pod disease in cacao. The sepecific objectives were to evaluate (1) effects of inoculum type and pod injury, (2) effects of inoculum type and seedling injury, and (3) effects of genetic background of cacao seedlings on infection of P. palmivora. In this experiment, effectiveness of either zoospora or mycellia was evaluated as inoculation sources for pod, leaf and stem of cacao clone GC 7 and Sca 12. Part of the tested cacao pods, leaves, and stems were injured prior to P. palmivora inoculation while the others were not. Observations were conducted on diameter of the necrosed symptoms on inoculated cacao pods, number of necrosed spot on inoculated leaf, and the length of necrosed symptoms on stem of tested cacao seedlings. Results of the experiment indicated inoculation using mycelia of P. palmivora was more effective than zoospora and injuring the tested cacao pods and seedlings before P. palmivora inoculation can be used to prediction the resistance of tested cacao clones against P. Palmivora infection. Results of resistance prediction using detached pod assay was similar to that of using seedling assay; therefore, seedling assay could be used as an alternattive method of resistance evaluation. Cacao F1 hybrids derived from TSH 858 x Sca 12 showed some resistance against P. palmivora and they might potentially result in high yielding lines.Key words: Phytophthora palmivora, cacao breeding, germplasm evaluation, inoculation standardization, pod assay, seedling assay.
Co-Authors , Agustiansyah . Agustiansyah ., Syamsuddin Abdul Munif Abubakar Ibrahim Agus Purwantara Agus Purwantara AGUS PURWANTARA Agus Purwantara Agustiansyah Agustiansyah Ahmad Zamzami Ainun Nur Maulidina Hikmawati Aminuyati ANAS D SUSILA Anas D. Susila Anwar, Aswaldi Aprizal Zainal Aris Hairmansis Atin Yulyatin Aulia Zakia Bagus Kukuh Udiarto Baharudin Baharudin ; BAHARUDIN BAHARUDIN Belladina Farhana Budhianto, Bambang Candra Budiman Dedy Suhendy DEVI RUSMIN Devi Rusmin DEVI RUSMIN Diah Manohara Dianawati, Meksy Dyah - Manohara DYAH MANOHARA Dyah Manohara Dyah Manohara Dyah Manohara Dyah Manohara E Widajati Edi Guhardja Edy Suprianto Endah Retno Palupi Endang Murniati Endang Pudjihartati Enni Suwarsi Rahayu Eny Widajati Faiza C Suwarno Faiza C Suwarno Farih Najah Rosadiah Giyanto . Giyanto, Giyanto HAJRIAL ASWIDINNOOR Hamami, Happy Suryati Hipi, Awaludin Ikrarwati, Ikrarwati IRENG DARWATI Kartika Kartika Lalu Firman Budiman M.R. SUHARTANTO M.R. SUHARTANTO, M.R. Manohara, Dyah - Maryati Sari MELATI MELATI Memen Surahman Mira Landep Widiastuti Mohamad Arif Mohamad Rahmad Suhartanto MR Suhartanto Muhammad Machmud Muhammad Machmud Muhammad Machmud Nelly Fridayanti Qadir, Abdul Rubiyo Rubiyo RUSMIN, DEVI Sholeh Avivi Sri Wilarso Budi Sudarsono Sudarsono . Sudarsono Sudarsono Sudarsono Sudarsono Sudarsono Sudarsono Sudarsono Sudarsono Susila, A D Syamsuddin Syamsuddin Syamsudin Syamsudin Trikoesoemaningtyas Trikoesoemaningtyas Wan Tin Diarni Wattimena, G A Yukti, AmiyarsI Mustika