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All Journal Jurnal Gizi dan Pangan Jurnal Akuakultur Indonesia ACTA VETERINARIA INDONESIANA Hemera Zoa Jurnal Sain Veteriner Jurnal Veteriner Statistika Jurnal Natur Indonesia Jurnal Ilmu Ternak Veteriner Journal of Innovation and Applied Technology EduChemia: Jurnal Kimia dan Pendidikan ANNALES BOGORIENSES Makara Journal of Science JUARA: JURNAL WAHANA ABDIMAS SEJAHTERA Jurnal Seni dan Reka Rancang: Jurnal Ilmiah Magister Desain E-JRM Jurnal Kedokteran Hewan Kohesi: Jurnal Sains dan Teknologi

Retno Damayanti Soejoedono

Department Of Animal Disease & Veterinary Public Health, Faculty Of Veterinary Medicine, IPB University, Bogor 16680, Indonesia

Author-ID : 2441329

Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Social Sciences Veterinary Other

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Identifikasi Listeria monocytogenes pada Susu Kambing di Kabupaten Purworejo Jawa Tengah Monika Danaparamitha Andriani; Trioso Purnawarman; Retno Damayanti; Syafril Daulay
Jurnal Sain Veteriner Vol 34, No 1 (2016): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Listeria monocytogenes is a pathogenic Listeria species, especially for high-risk groups and it that can be transmitted through contaminated food. Goat milk produced by traditional milking process and storage has a high risk of contamination. The aim of this study was to identify the presence of L. monocytogenes in fresh goat milk in Puworejo regency, Central Java. This study used 60 samples of raw goat milk that were obtained from seven farms by disease detection sampling method. All of the used method in this research refer to Indonesian National Standard (SNI) ISO 11290-1: 2012 about Microbiology of food and feed for detection and enumeration of Listeria monocytogenes. A total of 60 samples of raw goat milk that used in this study were not contaminatedwith L. monocytogenes. Based on the results of this study, it can be concluded that all the samples of raw goat milk were free from L. monocytogenes and have fulfilled the Indonesian National Standard (SNI) ISO No. 7388: 2009 about Limit of Microbial Contamination in Food.

Kertas Saring sebagai Media Transpor Darah untuk Pemeriksaan Antibodi Rabies Retno Wijayanti; Retno Damayanti; Sri Murtini
Jurnal Sain Veteriner Vol 37, No 2 (2019): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Rabies is a zoonotic disease. Rabies protection level detection was performed using antibody titration. Blood sampling activities in the field require special handling to avoid blood lysis, the sample delivery requires a cold chain with a stable temperature. Alternative method forwhole blood sample delivery using filter paper were carried out on 48 samples from susceptible animals (dogs and cats) transpored through the Soekarno Hatta Agricultural Quarantine Center. This study was designed to investigate the feasibility of filter paper sampling of blood at temperature of 26 °C to detect of rabies antibodies using indirect method of ELISA. The results of statistical analysis of Randomized Block Design and Tukey's test showed that the antibody titres of whole blood extracted from filter paper diluted in the PBS T 100 μl were equivalent to antibody titres of serum. Assesment of filter paper capability has sensitivity and specificity as much as 96% and 76%, positive predictive value of 67%, negative predictive value of 94% and reliability level of filter paper were 0.5 is moderate category. This indicates that the filter paper can be used as an alternative method of blood transport medium for rabies ELISA test.

Efektivitas Larutan Desinfektan Dalam Menginaktivasi Virus Avian Influenza pada Bulu Unggas Bagus Nanang Luwito; I Wayan Teguh Wibawan; Retno Damayanti Soejoedono
Jurnal Sain Veteriner Vol 36, No 2 (2018): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Avian Influenza (AI) virus is pathogenic agent that can spread from one area to another area through the transportation of infected animals or their products such as feathers. This research was aimed to inactivated AI virus with 775 ppm sodium hypochlorite and 0.1% chloroxylenol to examine the difference of treatment by time (day) for inactivation AI virus on the feathers. AI virus isolate A/chicken/sidrap/ 07160336‐2/2016 used in this research was obtained from Balai Besar Veteriner Maros. The treatment of disinfectants were performed on the first day (the day of disinfectan solutions were prepared), the third day and the seventh day by soaked the feathers in disinfectant solution for 10 minutes. The effectiveness of disinfectans were evaluated by inactivation index. The result show that the average of inactivation index of 775 ppm of sodium hypochlorit was 4.17 for the first day, 5.17 for the third day, and 4.20 for the seventh day, while the average of inactivation index of 0.1% chloroxylenol was 5.50 for the first day, 6.43 for the third day, and 5.77 for the seventh day. Our result indicated that the sodium hypochlorit and chloroxylenol were effective for inactivation of AI virus. The 0.1% of chloroxylenol was more effective for inactivation AI virus than 775 ppm of sodium hypochlorit, whilst the most effective duration for the treatment is the three days.

Kadar Protein Terlarut dalam Albumin Ikan Gabus (Channa striata dan Channa micropeltes) Asal Bogor SOLUBLE ROTEIN CONCENTRATION IN SNAKEHEAD FISH ALBUMIN BOGOR ORIGIN (CHANNA STRIATA AND CHANNA MICROPELTES) Rizky Alviodinasyari; Eko Sugeng Pribadi; Retno Damayanti Soejoedono
Jurnal Veteriner Vol 20 No 3 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Snakehead fish is currently used by the public as a health treatment because it has a higher content of albumin protein than others. The Research aimed was determined the protein quality and comparison levels in two species of snakehead fish, Channa striata and Channa micropeltes, derived from Bogor Regency. Protein concentration level of C. micropeltes and C. striata after drying for five minutes as much as 0.830 mg/mL and 0.803 mg/mL and these concentration were higher than dried for 10 and 15 minutes. Protein concentration levels of the two species were not significantly different. The molecular weight of albumin protein from both species were 65.56 kDa to dried for five minutes, 65.08 kDa to dried for 10 minutes, and 64.91 kDa to dried for 15 minutes. The Results concluded that there were no differences in protein levels in the fish meat and skin of the two species. Drying treatment affects the molecular weight of snakehead fish albumin protein.

Deteksi Coxiella burnetii Penyebab Q fever pada Sapi, Domba dan Kambing di Bogor dan Bali (DETECTION OF COXELLA BURNETII, THE CAUSAL AGENT OF Q FEVER Hapsari Mahatmi; Agus Setiyono; Retno Damayanti Soejoedono; Fachriyan Hasmi Pasaribu
Jurnal Veteriner Vol 8 No 4 (2007)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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A study to detect Coxiella burnetii, an intracellular bacterium causing Q fever in human and livestock animals, was carried out in several ruminants in Bogor and Bali. The methods used for the detection was Nested-Polymerase Chain Reaction (Nested-PCR). Two pairs of primers, the first (OMP1 and OMP2) and the second (OMP3 and OMP4) were used to detect the genomic sequences and the conserved specific sequences of Coxiella burnetii, respectively. Organ samples such as liver and lung from 410 livestock ruminants, consisting of cattle (245 samples), sheep (105 samples) and goats (60 samples) were collected from several slaughter houses in Bogor and Bali. As many as 15 (6.12%) out of 245 cattle, 6 (5.71%) out of 105 sheep and none from goat were infected by Coxiella burnetii. Interestingly, 3 out of 15 infected cattle were Bali cattle. The results clearly indicate that Q fever is likely to be widespread among ruminant animals in Indonesia.

POTENSI NETRALISASI IMUNOGLOBULIN Y ANTITETANUS YANG DIISOLASI DARI TELUR AYAM (THE POTENCY NETRALIZATION OF ANTI TETANUS IMMUNOGLOBULIN Y THAT WERE ISOLATED FROM CHICKEN EGGS) I Nyoman Suartha; I Wayan Teguh Wibawan; Retno Damayanti Soejoedono; Bibiana W. Lay
Jurnal Veteriner Vol 8 No 2 (2007)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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The porpuse of study was to explore the potential use of? anti tetanus IgY from eggs yolk as a substitute for anti tetanus serum raised in ?horses. The eggs were collected from chickens which have previously been immunized with tetanus toxoid. Neutralization potency test of anti tetanus IgY determined by ?Spearman-Karber method.? The highest mean titer of anti tetanus of egg yolk was 80.16 ? 33.55 IU/ml and the lowest was 1.69 ? 0.63 IU/ml. The concentration? of purified IgY was 1.644 ? 0.424 mg/ml. Spearman-Karber value of potency of anti tetanus IgY are 35 IU/ml. ?This research concluded that Chickens was capable of produced of anti tetanus in eggs yolk with value of potency are 35 IU/ml.

Uji Patogenisitas Zoospora Kapang Lagenidium giganteum terhadap Larva Instar-2 Nyamuk Aedes aegypti Skala Laboratorium (PATHOGENICITY TEST OF ZOOSPORA LAGENIDIUM GIGANTEUM FUNGI AGAINST AEDES AEGYPTI LARVAE 2nd UNDER LABORATORY CONDITION) Agustin Indrawati; Mirnawati Sudarwanto; Mangaraja Pidoli Tampubolon; Retno Damayanti Soejoedono; I Wayan Teguh Wibawan
Jurnal Veteriner Vol 12 No 1 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Dengue Haemorrhagic fever (DHF) is one of fearsome diseases in society. Incidence of the disease isincreasing. Dengue fever is caused by dengue virus and transmitted by Aedes aegypti mosquito vector.Various chemical controls have been conducted to prevent the spread of the disease, but active contents ofthe chemical controlling substances are suspected causing many negative effect, in environment, such asvector resistance, death of non target living creatures, and environmental contamination.This researchobjective was to find an alternative solution in order to control the dengue vector by using entomopathogenicfungi as biological control agent. This research was conducted by isolation and identification of fungiinfecting mosquito larvae. Macroscopic observation revealed that one of the nine isolation products wasLagenidium giganteum. The effectiveness test in laboratory showed the zoospore LD50 to Ae.aegypti larvaeof instar 2nd was 2,35 x 106 zoospore/ml, while the LD95 value was 1,35 x 107 zoospore/ml. The oosporeeffectiveness test showed LD50 was 6,7 x 102 oospore/ml and LD95 was 1,94 x 103 oospore/ml. Using LPCBdye and blue tolouidin 2,5%, the infection mechanism of L.giganteum fungi in Ae.aegypti mosquito larvawas detected. The research is concluded that the entomophatogen fungi L. giganteum was very prospectiveto be used as a biological control agent against vector of DHF.

Preparasi Imunoglobulin G Kelinci sebagai Antigen Penginduksi Antibodi Spesifik Terhadap Virus Avian Influenza H5N1 Strain Legok Ketut Karuni Nyanakumari Natih; Retno Damayanti Soejoedono; I Wayan Teguh Wibawan; Fachriyan Hasmi Pasaribu
Jurnal Veteriner Vol 11 No 2 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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The aim of this research was to prepare rabbit Immunoglobulin G as anti-idiotype antibody (Ab2) ofAvian Influenza Virus (AIV) H5N1. A polyclonal antibody was collected from guinea pigs immunized withinactivated AI vaccine H5N1of Legok strain. Antibody of H5N1 AI in serum was detected by Agar gelprecipitation test (AGPT) and an Inhibition Hemmaglutination test (IHT). The highest titre of antibodywas obtained one week after the third immunization. Serum of guinea pigs containing IgG was purifiedusing the Montage Antibody purification kit & spin column with Prosep A media (Millipore). The AI H5N1IgG concentration was 8 mg/ml. AI H5N1 IgG, was then digested with pepsin to obtain F(ab)2 fraction andwas called Ab1. The concentration of IgG and F(ab)2 and purity of IgG were determined by UVspectrophotometer which showed Ab1 concentration 1 mg/ml. Molecular weight was estimated by sodiumdodecyl sulfate- polyacrilamide gel electrophoresis (SDS-PAGE). Ab2 was produced by immunization ofrabbit with Ab1. The first immunization was carried out by subcutaneous injection with 500 ?g of Ab1emulsified in Complete Freund Adjuvant. The immunization was repeated with the same dose of Ab1emulsified in Incomplete Freund Adjuvan at 1 week intervals. One week after the second immunization,rabbit’s serum was harvested and IgG was purified using the Montage Antibody purification kit & spincolumn with Prosep A media (Millipore). The rabbit IgG, called Ab2, was an anti-idiotypic antibody againstAIV-H5N1. In AGPT, a precipitation line appeared between Ab1 and Ab2. A partial reaction appearedbetween Ab2 and the AI H5N1 antigen was also detected. The results indicated that Ab2 is a possiblecandidate of imunogen for protection against an AI virus H5N1 infection.

Kemampuan Daya Serap Zeolit Sintetis Dibandingkan dengan Serpentin Teraktifasi Terhadap Gas CO2 M. Lutfi; Harry Tetra Antono; Agus Wahyudi; Retno Damayanti
STATISTIKA: Forum Teori dan Aplikasi Statistika Vol 16, No 2 (2016)
Publisher : Program Studi Statistika Unisba

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29313/jstat.v16i2.2290

Kebutuhan energi yang semakin meningkat merupakan salah satu penyebab peningkatankonsentrasi CO2 di atmosfir. Penelitian tentang penangkapan CO2 telah banyak dilakukan, beberapamaterial dapat dipakai sebagai adsorben CO2. Kegiatan yang dilakukan meliputi pembuatan bahanpenyerap yang berasal dari beberapa material/mineral, yaitu zeolit sintetik dan aktivasi serpentin;perancangan alat simulasi dan uji coba penyerapan CO2. Kedua material tersebut kemudian diujikansebagai material penyerap/adsorben gas CO2 pada alat simulasi penyerapan CO2. Hasil serapantertinggi gas CO2 menggunakan zeolit sintetik adalah 13.98% (3.92 g/g) dengan jumlah zeolit yangdigunakan sebesar 5 g, sedangkan bila menggunakan serpentin teraktivasi adalah 14.3 % (5.13 g/g)dengan jumlah serpentin yang digunakan sebesar 5 g, keduanya dapat digunakan sebagai bahanpenyerap CO2.

Construction and Expression of Immunotoxin Anti EGFRvIII scFv-HPR Conjugate in Pichia pastoris as A Targeted Drug Candidate for Cancer Therapy Yuliawati Yuliawati; Retno Damayanti Soejoedono; Asrul Muhamad Fuad
ANNALES BOGORIENSES Vol 18, No 1 (2014): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Epidermal growth factor receptor variant III (EGFRvIII) is a mutant of EGFR lacking 267 amino acids (exon-2 through 7) within its extracellular domain, resulting in the formation of a new epitope as a tumorspecific target. EGFRvIII is commonly found in GBM, breast, ovarian, prostate, and lung carcinomas. Antibodies or part of antibodies (e.g. single chain variable fragment or scFv) with specific activity against this receptor have been developed. These antibodies are internalized into the cell after receptor binding. Ribonucleases can be cytotoxic due to their inherent ability to degrade RNA, therefore causing cell death via inhibition of protein synthesis. The aim of this research is to construct, clone and express an immunotoxin-based conjugate combining an anti-EGFRvIII scFv with a HPRmut (human pancreatic ribonuclease mutant variant) in Pichia pastoris. The anti-EGFRvIII scFv gene was cloned into yeast expression vector pPICZαA and fused with EGFP gene as a marker. The HPRmut gene was subsequently cloned at the C-terminal of the scFv::EGFP fusion, resulting in the scFv::EGFP::HPR fusion construct. The fusion construct was successfully obtained and nucleotide sequence of plasmid was verified. This construct was used to transform Pichia pastoris SMD 1168H. The gene fusion was successfully transformed and expressed in P. pastoris with a transformation efficiency of 102 cfu/μg DNA. The transformed yeasts were screened on agar media containing up to 1000 μg/ml zeocin. Yeast transformants showed green fluorescent due to the expression of EGFP gene. The recombinant proteins have been expressed and secreted from P. pastoris as showed by immunoblotting and SDS-PAGE analyses, then purified by affinity chromatography method. The selected yeast transformant produced at least 15.85 mg of purified protein per liter of yeast culture.

Co-Authors . Darmawi Achmad Agus Priyono Adji, Rahmat Setya Agus Setiyono Agus Wahyudi Agustin Indrawati Anneke Anggraeni Anugrah Nur Rahmanto Asrul Muhamad Fuad Asrul Muhamad Fuad, Asrul Muhamad Assaat, Lusiani Dewi Ayi Santika Bagas Setya Dian Nugraha Bagus Nanang Luwito Bambang Susilo BIBIANA W LAY Dewi Elfidasari Dwi Desmiyeni Putri Ekowati Handharyani Emilia A Erick Teguh Leksono Etih Sudarnika Evy Damayanthi Fachriyan Hasmi Pasaribu Fachriyan Hasmi Pasaribu Fachriyan Hasymi Pasaribu Fajriah, Sofa Fitrianti Rahayu Hadri Latif Hana Fitria Navratilova Handika Martu Kurniawan Hapsari Mahatmi Harry Tetra Antono I Nyoman Suartha I wayan Teguh Wibawan Intan Nur Fitriyana Irvan Faizal Iskandar, Layla Nurina Kartika Ivandini Tribidasari Anggraningrum Ketut Karuni Nyanakumari Natih Komar Sumantadinata Lilis Suryani M. Lutfi Maggy Thenawidjaja Suhartono Makmur iknu wijaya Mangaraja Pidoli Tampubolon Melani Wahyu Adiningsih Mirnawati Sudarwanto Monika Danaparamitha Andriani Muhammad Hambal Natih, Ketut Karuni Nyanakumari Ni Luh Putu Ika Mayasari Nova Dilla Yanthi Novera Nirmalasanti Nurhidayah Nurhidayah Okti Nadia Poetri R.A. Heryani Wahyuningrum Rahmat Setya Adji Ramlah Ramlah Razali Daud Retno Wijayanti Risa Tiuria Risma Juniarti Silitonga Rizky Alviodinasyari Romadhony Ardiansyah Rudy Ariyanto Saepudin, Endang Sandra Sandra Siti Gusti Ningrum, Siti Gusti sri murtini . Sri Nuryati Suherman . Sukenda . Surachmi Setiyaningsih Sutanti, , Syafril Daulay Syahruddin Said TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN Trioso Purnawarman Ummu Balqis Usman Nurhasan Wijaya, Makmur Yuliawati Yuliawati Yus Rusila Noor Yus Rusila Noor Yusuf Hendrawan

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