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All Journal Jurnal Gizi dan Pangan Jurnal Akuakultur Indonesia ACTA VETERINARIA INDONESIANA Hemera Zoa Jurnal Sain Veteriner Statistika Jurnal Natur Indonesia Jurnal Ilmu Ternak Veteriner Journal of Innovation and Applied Technology EduChemia: Jurnal Kimia dan Pendidikan ANNALES BOGORIENSES Makara Journal of Science JUARA: JURNAL WAHANA ABDIMAS SEJAHTERA Jurnal Seni dan Reka Rancang: Jurnal Ilmiah Magister Desain E-JRM Jurnal Kedokteran Hewan Kohesi: Jurnal Sains dan Teknologi Annales Bogorienses

Retno Damayanti Soejoedono

Department Of Animal Disease & Veterinary Public Health, Faculty Of Veterinary Medicine, IPB University, Bogor 16680, Indonesia

Author-ID : 2441329

Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Social Sciences Veterinary Other

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Kertas Saring sebagai Media Transpor Darah untuk Pemeriksaan Antibodi Rabies Retno Wijayanti; Retno Damayanti; Sri Murtini
Jurnal Sain Veteriner Vol 37, No 2 (2019): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Rabies is a zoonotic disease. Rabies protection level detection was performed using antibody titration. Blood sampling activities in the field require special handling to avoid blood lysis, the sample delivery requires a cold chain with a stable temperature. Alternative method forwhole blood sample delivery using filter paper were carried out on 48 samples from susceptible animals (dogs and cats) transpored through the Soekarno Hatta Agricultural Quarantine Center. This study was designed to investigate the feasibility of filter paper sampling of blood at temperature of 26 °C to detect of rabies antibodies using indirect method of ELISA. The results of statistical analysis of Randomized Block Design and Tukey's test showed that the antibody titres of whole blood extracted from filter paper diluted in the PBS T 100 μl were equivalent to antibody titres of serum. Assesment of filter paper capability has sensitivity and specificity as much as 96% and 76%, positive predictive value of 67%, negative predictive value of 94% and reliability level of filter paper were 0.5 is moderate category. This indicates that the filter paper can be used as an alternative method of blood transport medium for rabies ELISA test.

Efektivitas Larutan Desinfektan Dalam Menginaktivasi Virus Avian Influenza pada Bulu Unggas Bagus Nanang Luwito; I Wayan Teguh Wibawan; Retno Damayanti Soejoedono
Jurnal Sain Veteriner Vol 36, No 2 (2018): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Avian Influenza (AI) virus is pathogenic agent that can spread from one area to another area through the transportation of infected animals or their products such as feathers. This research was aimed to inactivated AI virus with 775 ppm sodium hypochlorite and 0.1% chloroxylenol to examine the difference of treatment by time (day) for inactivation AI virus on the feathers. AI virus isolate A/chicken/sidrap/ 07160336‐2/2016 used in this research was obtained from Balai Besar Veteriner Maros. The treatment of disinfectants were performed on the first day (the day of disinfectan solutions were prepared), the third day and the seventh day by soaked the feathers in disinfectant solution for 10 minutes. The effectiveness of disinfectans were evaluated by inactivation index. The result show that the average of inactivation index of 775 ppm of sodium hypochlorit was 4.17 for the first day, 5.17 for the third day, and 4.20 for the seventh day, while the average of inactivation index of 0.1% chloroxylenol was 5.50 for the first day, 6.43 for the third day, and 5.77 for the seventh day. Our result indicated that the sodium hypochlorit and chloroxylenol were effective for inactivation of AI virus. The 0.1% of chloroxylenol was more effective for inactivation AI virus than 775 ppm of sodium hypochlorit, whilst the most effective duration for the treatment is the three days.

Kemampuan Daya Serap Zeolit Sintetis Dibandingkan dengan Serpentin Teraktifasi Terhadap Gas CO2 M. Lutfi; Harry Tetra Antono; Agus Wahyudi; Retno Damayanti
STATISTIKA: Forum Teori dan Aplikasi Statistika Vol 16, No 2 (2016)
Publisher : Program Studi Statistika Unisba

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29313/jstat.v16i2.2290

Kebutuhan energi yang semakin meningkat merupakan salah satu penyebab peningkatankonsentrasi CO2 di atmosfir. Penelitian tentang penangkapan CO2 telah banyak dilakukan, beberapamaterial dapat dipakai sebagai adsorben CO2. Kegiatan yang dilakukan meliputi pembuatan bahanpenyerap yang berasal dari beberapa material/mineral, yaitu zeolit sintetik dan aktivasi serpentin;perancangan alat simulasi dan uji coba penyerapan CO2. Kedua material tersebut kemudian diujikansebagai material penyerap/adsorben gas CO2 pada alat simulasi penyerapan CO2. Hasil serapantertinggi gas CO2 menggunakan zeolit sintetik adalah 13.98% (3.92 g/g) dengan jumlah zeolit yangdigunakan sebesar 5 g, sedangkan bila menggunakan serpentin teraktivasi adalah 14.3 % (5.13 g/g)dengan jumlah serpentin yang digunakan sebesar 5 g, keduanya dapat digunakan sebagai bahanpenyerap CO2.

Construction and Expression of Immunotoxin Anti EGFRvIII scFv-HPR Conjugate in Pichia pastoris as A Targeted Drug Candidate for Cancer Therapy Yuliawati Yuliawati; Retno Damayanti Soejoedono; Asrul Muhamad Fuad
ANNALES BOGORIENSES Vol 18, No 1 (2014): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Epidermal growth factor receptor variant III (EGFRvIII) is a mutant of EGFR lacking 267 amino acids (exon-2 through 7) within its extracellular domain, resulting in the formation of a new epitope as a tumorspecific target. EGFRvIII is commonly found in GBM, breast, ovarian, prostate, and lung carcinomas. Antibodies or part of antibodies (e.g. single chain variable fragment or scFv) with specific activity against this receptor have been developed. These antibodies are internalized into the cell after receptor binding. Ribonucleases can be cytotoxic due to their inherent ability to degrade RNA, therefore causing cell death via inhibition of protein synthesis. The aim of this research is to construct, clone and express an immunotoxin-based conjugate combining an anti-EGFRvIII scFv with a HPRmut (human pancreatic ribonuclease mutant variant) in Pichia pastoris. The anti-EGFRvIII scFv gene was cloned into yeast expression vector pPICZαA and fused with EGFP gene as a marker. The HPRmut gene was subsequently cloned at the C-terminal of the scFv::EGFP fusion, resulting in the scFv::EGFP::HPR fusion construct. The fusion construct was successfully obtained and nucleotide sequence of plasmid was verified. This construct was used to transform Pichia pastoris SMD 1168H. The gene fusion was successfully transformed and expressed in P. pastoris with a transformation efficiency of 102 cfu/μg DNA. The transformed yeasts were screened on agar media containing up to 1000 μg/ml zeocin. Yeast transformants showed green fluorescent due to the expression of EGFP gene. The recombinant proteins have been expressed and secreted from P. pastoris as showed by immunoblotting and SDS-PAGE analyses, then purified by affinity chromatography method. The selected yeast transformant produced at least 15.85 mg of purified protein per liter of yeast culture.

Pengaruh Financial Literacy, Trus Dan Image Terhadap Behaviour Intention To Use Fintech (Studi Pada Mahasiswa Prodi Manajemen Angkatan 2018 Fakultas Ekonomi dan Bisnis Universitas Islam Malang) Retno Damayanti; Nurhidayah Nurhidayah; Achmad Agus Priyono
E-JRM : Elektronik Jurnal Riset Manajemen eJrm Vol. 11 No. 04 Februari 2022
Publisher : UNIVERSITAS ISLAM MALANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (509.212 KB)

Abstract This study aims to determine the effect of Financial Literacy, Trust and Image on Behavior Intention to use Fintech in Management Study Program Students Class of 2018 Faculty of Economics and Business, Islamic University of Malang. Methods of analysis using multiple linear regression analysis. The population in this study were Active Students of Management Study Program Class of 2018 Faculty of Economics and Business, Islamic University of Malang, which was obtained as many as 518 students. In this study, the sample selection used the Slovin formula with purposive sampling technique, in order to obtain 84 students who met the criteria. The results of this study indicate that (1) Financial Literacy has no significant effect on Behavior Intention to use Fintech, (2) Trust has a significant and positive effect on Behavior Intention to use Fintech, (3) Image has a significant and positive effect on Behavior Intention to use Fintech. . Keywords : Financial Literacy, Trust, Image, Behavior intention to use Fintech.

RESPONS ANTIBODI AYAM PETELUR YANG DIBERIKAN PROTEIN EKSKRETORI/SEKRETORI DAN DITANTANG DENGAN TELUR INFEKTIF Ascaridia galli Darmawi D; Ummu Balqis; Risa Tiuria; Retno Damayanti Soejoedoeno; Fachriyan Hasymi Pasaribu; Muhammad Hambal; Razali Daud
Jurnal Kedokteran Hewan Vol 7, No 2 (2013): September
Publisher : Universitas Syiah Kuala

Penelitian ini bertujuan mengetahui respons antibodi dalam serum ayam petelur terhadap ekskretori/sekretori, dan ditantang dengan telur infektif Ascaridia galli (A. galli) Sebanyak 12 ekor ayam dibagi dalam empat kelompok. Kelompok pertama adalah ayam yang tidak diimunisasi dan tidak diinfeksi (kontrol), kelompok kedua adalah ayam yang diimunisasi dengan dosis 260 µg ekskretori/sekretori larva A. galli, kelompok ketiga adalah ayam yang diinfeksi dengan dosis 1000 telur infektif A. galli, dan kelompok keempat adalah ayam yang diimunisasi dengan dosis 260 µg ekskretori/sekretori dan satu minggu kemudian ditantang dengan dosis 1000 telur infektif A. galli. Respons antibodi pada masing-masing kelompok dianalisis dengan uji enzymelinkedimmunosorbantassay (ELISA) setiap satu minggu selama 10 minggu pascainfeksi. Hasil penelitian menunjukkan bahwa imunisasi dan atau infeksi dapat memicu peningkatan titer antibodi serum secara signifikan (P0,05) selama 10 minggu pascainfeksi. Titer tertinggi adalah 2,63±1,20 OD (optical density) dicapai pada minggu ke-3 pascainfeksi dan titer terendah adalah 1,51±0,48 OD pada minggu ke-0. Ekskretori/sekretori dapat memicu respons antibodi serum ayam petelur terhadap A. galli.

ISOLASI DAN KARAKTERISASI BIOLOGIK VIRUS NEWCASTLE DISEASE Emilia A; Surachmi Setiyaningsih; Retno Damayanti Soejoedono
Jurnal Kedokteran Hewan Vol 9, No 1 (2015): March
Publisher : Universitas Syiah Kuala

Tujuan dari penelitian ini adalah memperoleh informasi infeksi dan sifat-sifat biologis virus Newcastle disease (ND) pada ayam ras maupunayam buras. Sejumlah 529 sampel usap kloaka dikoleksi dari pasar-pasar tradisional dan peternakan rakyat di daerah Bogor dan Tangerang. Sebanyak 20 sampel terdeteksi positif melalui real time reverse transcription-polymerase chain reaction (RRT-PCR) sedangkan dengan isolasi virus terdeteksi positif sebanyak 11 sampel. Selanjutnya empat isolat representasi lokasi dipilih untuk karakterisasi patogenisitas dengan metode mean death time (MDT) pada telur ayam berembrio (TAB) specific pathogen free (SPF), dan karakterisasi antigenisitas menggunakan metode haemagglutination inhibition (HI) dan virus netralisasi (VN) untuk melihat keragaman antar isolat virus. Uji MDT memperlihatkan dua isolat(kode isolat II dan XIII) adalah mesogenik, satu isolat (kode isolate VIII) termasuk lentogenik, dan satu isolat (kode isolat TW) bersifat velogenic. Tiga dari empat isolat menunjukkan hubungan antigenik dengan virus ND strain Komarov dan G7 (genotipe 7) tetapi tidak dengan strain referensi Lasota, sementara isolat velogenic (kode TW) yang berasal dari ayam kampung menunjukkan reaksi silang tinggi dengan antiserum terhadap tiga strain referensi di atas. Sampel-sampel yang diambil di lapang baik itu berasal dari pasar dan peternakan rakyat meskipun secara klinis tidak memperlihatkan gejala sakit dapat diisolasi virus ND dengan karakteristik yang beragam.

Identification of Gene Resistance to Avian Influenza Virus (Mx Gene) among Wild Waterbirds Elfidasari, Dewi; Solihin, Dedy Duryadi; Soejoedono, Retno Damayanti; Murtini, Sri
Makara Journal of Science Vol. 17, No. 1
Publisher : UI Scholars Hub

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The Mx gene is an antiviral gene used to determine the resistance or the susceptibility to different types of viruses, including the Avian Influenza (AI) virus subtype H5N1. The AI virus subtype H5N1 infection in chickens causes Mx gene polymorphism. The Mx+ gene shows resistant to the AI virus subtype H5N1, whereas the Mxgene shows signs of susceptible. The objective of this research was to detect the Mx gene in wild aquatic birds using the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method with the primer pairs F2 and NE-R2/R and the RsaI restriction enzyme. DNA samples were obtained from eight species of wild waterbirds with positive and negative exposure to the AI virus subtype H5N1. DNA amplification results showed that the Mx gene in wild aquatic birds is found in a 100 bp fragment, which is the same as the Mx gene found in chickens. However, unlike chickens, the Mx gene in wild aquatic birds did not show any polymorphism. This study proves that Mx- based resistance to AI virus subtype H5N1 in different in wild birds than in chickens.

SEROSURVEILLANCE OF AVIAN INFLUENZA VIRUS SUBTYPE H5N1 WITH HAEMAGGLUTINATION-INHIBITION ON WILD AQUATIC BIRDS IN PULAU DUA SERANG NATURAL RESERVES, BANTEN PROVINCE Elfidasari, Dewi; Solihin, Dedy Duryadi; Soejoedono, Retno Damayanti; Murtini, Sri; Noor, Yus Rusila
Makara Journal of Science Vol. 15, No. 2
Publisher : UI Scholars Hub

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Further detailed research is required to obtain deeper information on the role of wild birds on the distribution of Avian influenza in Asia. A research has been carried out on February–June 2007 focused on blood sampling (serosurveillance) of wild birds in Pulau Dua Nature Reserves (CAPD), Serang, Banten. The research is aimed to investigate the infection of AI virus sub-tye H5N1 on the studied wild birds. The blood samples were taken from studied aquatic birds, followed by HI (haemagglutination-inhibition) test. A total of 183 samples represent 7 water bird species were taken i.e Cattle egret Bubulcus ibis, Javan pond-heron Ardeola speciosa, Little egret Egretta garzetta, Intermediate egret Egretta intermedia, Black-crowned night heron Nycticorax nycticorax, Great egret Casmerodius albus and Grey heron Ardea cinerea. The result revealed that 41 (23.27%) samples showed the present of AIV antibodies serotype H5N1 which is identified as positive. Data showed 5 positive-test species, including B. ibis (29.27%), E. garzetta (29.27%), E. intermedia (4.88%), Ardeola speciosa (7.32%), and N. nycticorax (29.27%). A total of 41.46% were infected adult individual, whereas 58.54% were juveniles.

Chicken Immune Responses to Vaccination by the Avian Influenza Subtype H5N1 against Avian Influenza Ramlah, Ramlah; Murtini, Sri; Soejoedono, Retno Damayanti; Natih, Ketut Karuni Nyanakumari; Ningrum, Siti Gusti
Makara Journal of Science Vol. 27, No. 3
Publisher : UI Scholars Hub

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Avian Influenza (AI) is one of the strategic animal diseases still considered a priority for control by the Indonesian government. This study aimed to examine the immune response of chickens to various types of H5N1 subtype AI vaccines in Indonesia and to determine the correlation of factors influencing the post-vaccination AI antibody response. Serum samples were tested using the hemagglutination inhibition (HI) test with the standard AI antigen subtype H5N1 strain A/Chicken/Barru/BBVM/41-13/2013 (Clade 2.1.3) and strain A/Chicken/Semarang/04141225-07/2014 (Clade 2.3.2). Antibody titer was calculated using the Geometric Mean Titer (GMT). The correlation analyses were performed to assess the correlation of antibody titer against each of the following factors: age of chicken at the time of sampling, the interval between sampling time and the previous vaccination, and the number of vaccinations performed. The results showed that the average antibody titer value against the AI antigen subtype H5N1 strain A/Chicken/Barru/BBVM/41-13/2013 (Clade 2.1.3) was highest in samples from the South Sumatra Province, namely, Palembang City, which was 26.42 HIU. The results showed a significant correlation (p-value <0,05) between antibody titer and the interval between sampling time and the previous vaccination, the number of vaccination performed and age of chicken at the time of sampling. Therefore, results showed that the immunity developed from vaccination using the AI vaccine seed subtype H5N1 could induce immunity with a protective value of ≥16.

Co-Authors . Darmawi Achmad Agus Priyono Adji, Rahmat Setya Agus Setiyono Agus Wahyudi Agustin Indrawati Anneke Anggraeni Anugrah Nur Rahmanto Ardiansyah, Romadhony Asrul Muhamad Fuad Asrul Muhamad Fuad, Asrul Muhamad Assaat, Lusiani Dewi Ayi Santika Bagas Setya Dian Nugraha Bagus Nanang Luwito Bambang Susilo BIBIANA W LAY Darmawi D Dewi Elfidasari Dwi Desmiyeni Putri Ekowati Handharyani Emilia A Emilia A, Emilia Erick Teguh Leksono Etih Sudarnika Evy Damayanthi Fachriyan Hasmi Pasaribu Fachriyan Hasmi Pasaribu Fachriyan Hasymi Pasaribu Fachriyan Hasymi Pasaribu Fajriah, Sofa Fitrianti Rahayu Fitriyana, Intan Nur Hadri Latif Hana Fitria Navratilova Handika Martu Kurniawan Hapsari Mahatmi Harry Tetra Antono I Nyoman Suartha I wayan Teguh Wibawan Irvan Faizal Iskandar, Layla Nurina Kartika Ivandini Tribidasari Anggraningrum Ketut Karuni Nyanakumari Natih Komar Sumantadinata Lilis Suryani M. Lutfi Maggy Thenawidjaja Suhartono Makmur iknu wijaya Mangaraja Pidoli Tampubolon Melani Wahyu Adiningsih Mirnawati Sudarwanto Monika Danaparamitha Andriani Muhammad Hambal Natih, Ketut Karuni Nyanakumari Ni Luh Putu Ika Mayasari Nova Dilla Yanthi Novera Nirmalasanti Nurhidayah Nurhidayah Okti Nadia Poetri R.A. Heryani Wahyuningrum Rahmat Setya Adji Ramlah Ramlah Razali Daud Razali Daud Retno Wijayanti Risa Tiuria Risma Juniarti Silitonga Rizky Alviodinasyari Rudy Ariyanto Saepudin, Endang Sandra Sandra Siti Gusti Ningrum, Siti Gusti sri murtini . Sri Nuryati Suherman . Sukenda . Surachmi Setiyaningsih Sutanti, , Syafril Daulay Syahruddin Said TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN Trioso Purnawarman Ummu Balqis Usman Nurhasan Yuliawati Yuliawati Yuliawati Yus Rusila Noor Yus Rusila Noor Yusuf Hendrawan

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