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All Journal Agrikultura Zuriat Jurnal Fakultas Pertanian Buletin Plasma Nutfah JURNAL AGROTEKNOLOGI Planta Tropika Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) Kultivasi Jurnal Sains Teh dan Kina Chimica et Natura Acta Syntax Literate: Jurnal Ilmiah Indonesia Jurnal Riset Akuakultur AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Jurnal Teknika Budapest International Research and Critics Institute-Journal (BIRCI-Journal): Humanities and Social Sciences Jurnal Indonesia Sosial Teknologi Perwira Journal of Economics and Business (PJEB) Perwira Journal of Community Development (PJCD) TarbiyahMU

Nono Carsono

Fakultas Pertanian Universitas Padjadjaran

Author-ID : 246005

Religion Agriculture, Biological Sciences & Forestry Arts Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Electrical & Electronics Engineering Environmental Science Industrial & Manufacturing Engineering Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mechanical Engineering Social Sciences Other

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Seleksi Padi dengan Kandungan Amilosa Sedang Menggunakan Marka Molekuler dan Pengujian Fenotipik Whitea Yasmine Slamet; Anita Rachmasari S.W.; Santika Sari; Nono Carsono
Zuriat Vol 29, No 2 (2018): Zuriat Vol. 29 No. 2 (Desember 2018)
Publisher : Breeding Science Society of Indonesia (BSSI) / PERIPI

Padi dengan kandungan amilosa sedang sangat diminati oleh masyarakat Asia dan Dunia. Tujuan dari penelitian ini adalah untuk memperoleh individu keturunan F7 (yang berasal dari hasil persilangan Sintanur x PTB33 dan Pandanwangi x PTB33) dengan kandungan amilosa sedang. Alat yang digunakan untuk analisis kandungan amilosa yaitu spektrofotometer dengan panjang gelombang 625 nm, sedangkan marka molekuler menggunakan marka RM190, SSIIa dan Wx. Hasil dari penelitian ini menunjukkan bahwa SP73-3-1-7 memiliki jumlah tanaman paling banyak yang mengandung amilosa yang sedang berdasarkan marka molekuler dan fenotipik. Kombinasi kedua pengujian ini akurat dalam menyeleksi karakter fisikokimia padi.

Gene Transfer to Rice Mediated by Agrobacterium tumefaciens: Transient Expression of sgfp in Rice Calluses of indica cv. Fatmawati and japonica cv. Nipponbare Tomohiko Yoshida; Nono Carsono
Zuriat Vol 19, No 1 (2008)
Publisher : Breeding Science Society of Indonesia (BSSI) / PERIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/zuriat.v19i1.6694

Transient expression of synthetic green fluorescent protein (sgfp) mediated by Agrobacterium is rapid and useful approach for visual monitoring the genetic transformation event in transformed cells/tissues of examined genotype. The significant differences in transient expression of two genotypes (indica cv. Fatmawati and japonica cv. Nipponbare) were found with regard to the osmotic treatment (0.4 M mannitol), solid subcultured callus, and 10 min. air drying. While, no significant differences in sgfp expression were observed in two genotypes on without air-drying and 5 min. air-drying of calluses prior immersed in Agrobacterium suspension. Surprisingly, the sgfp expression could not be detected in suspension-subcultured callus of both cultivars. The highest sgfp expression was achieved in Nipponbare callus treated with 10 min. air drying. The level of green fluorescent spots was higher in Nipponbare than that in Fatmawati, however, plants regenerated from Fatmawati were considerably comparable with those of Nipponbare. Seventeen and 16 putative transgenic rice plants expressing sgfp transgene were obtained from Nipponbare and Fatmawati, respectively.

The Perception of High School Communities towards River Restoration Program Atie Tri Juniati; Dwi Ariyani; Nuryani Tinumbia; Fadli Kurnia; Atri Prautama Dewi; Gatut Bayuaji; Hera Hera; Nono Carsono
Jurnal Pengabdian kepada Masyarakat (Indonesian Journal of Community Engagement) Vol 7, No 4 (2021): December
Publisher : Direktorat Pengabdian kepada Masyarakat Universitas Gadjah Mada

River restoration is an activity to restore the river's condition to its original state. River restoration is one of the SDGs targets, namely target 6.6, Aquatic Ecosystems.The target of 6.6 SDGs is to conserve and restore water-related ecosystems such as mountains, forests, wetland vegetation, rivers, reservoirs and lakes. These SDGs targets will be successful with the participation of the surrounding community. In order to pursue this target, the Civil Engineering Study Program, Pancasila University has organized a community service program related to river restoration activities, in the Cimanuk-Cisanggarung river area, Cirebon. Community service activities are carried out by providing counseling about the importance of river restoration programs to high school teachers and assessing school readiness to form a water quality monitoring school partnership. To assess the understanding of river restoration and the readiness to establish partnership, a questionnaire was given. Based on the questionnaire analysis, it can be concluded that most of the participants (76%) stated that the river restoration program was important, and would be ready to partner with other schools (24%). This shows that program respondents are ready to form partnerships to support river restoration programs.

KONSTRUKSI VEKTOR EKSPRESI GEN HORMON PERTUMBUHAN LELE DUMBO (Clarias sp.) UNTUK PRODUKSI IKAN LELE LOKAL (Clarias batrachus) TRANSGENIK Ibnu Dwi Buwono; Nono Carsono; M. Untung Kurnia
Jurnal Riset Akuakultur Vol 10, No 1 (2015): (Maret 2015)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Penelitian ini bertujuan untuk memperoleh konstruksi vektor ekspresi (pTarget) rekombinan yang mengandung sisipan gen hormon pertumbuhan ikan lele dumbo (Clarias sp.) dan promoter β-aktin ikan lele lokal (C. batrachus) dalam upaya pembuatan ikan lele lokal transgenik. Promoter β-aktin lele lokal (pCbBA) telah berhasil diisolasi dari hipofisa ikan tersebut dengan ukuran sekitar 1,7 kbp; dan memiliki elemen transkripsi CAAT box, TATA box, GC box, motif CarG, dan TGACG berdasar analisis program TF Bind. Penggantian promoter CMV (cytomegalovirus) yang terkandung dalam vektor ekspresi pTarget menggunakan dua enzim restriksi SgfI dan I-PpoI, menghasilkan fragmen DNA berukuran 6.083 bp (pTarget-GH lele dumbo) sebagai produk digesti. Fragmen pTarget-GH lele dumbo yang diligasi dengan promoter β-aktin lele lokal membentuk konstruksi By: Ibnu Dwi Buwono, Nono Carsono, Yuniar Mulyani, and M. Untung KurniaThis study aims to obtain an expression vector construct (pTarget) containing recombinant growth hormone gene insertion of African catfish (Clarias sp.) and β-actin promoter derived from walking catfish (Clarias batrachus) in order to produce transgenic local catfish. β-actin promoter of walking catfish (pCbBA) have been isolated from the pituitary of the fish with a size of about 1.7 kbp, and has a transcription element: CAAT box, TATA box, GC box, CarG, and TGACG motif based on analysis result of TF Bind program. Replacement of CMV (cytomegalovirus) promoter contained in the expression vector pTarget using restriction enzymes SgfI and I-PpoI, obtained a product of digestion with the fragment size of 6,083 bp (pTarget-GH African catfish). pTarget-GH fragments were ligated with the African catfish β-actin promoter to arrange a construct of pTarget-pCbBA-African catfish GH (7,783 bp) as transgenic walking catfish expression vector.

ISOLASI DAN ANALISIS GEN HORMON PERTUMBUHAN LELE (Clarias gariepinus Burch.) Ibnu Dwi Buwono; Nono Carsono; Yuniar Mulyani
Jurnal Riset Akuakultur Vol 7, No 3 (2012): (Desember 2012)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Isolasi gen hormon pertumbuhan lele dumbo (C. gariepinus) dari hipofisa ikanmerupakan sumber DNA yang selanjutnya dapat digunakan sebagai cetakan untuk mengkopi gen tersebut menggunakan primer Amc-GH-F dan Amc-GH-R. Fragmen DNA lele dumbo hasil isolasi terdeteksi berdasar elektroforesis gel agarosa 1%. Amplifikasi gen penyandi hormon pertumbuhan (Growth Hormone / GH) lele dumbo menggunakan primer Amc-GH-F (5’-GCAGAAATGGCTCGAGGTAAGG-3’) dan Amc-GH-R (5’-CAGGGTGCAGTTGGAATCC-3’) dapat mengkopi sekuen gen GH lele dengan ukuran fragmen PCR sekitar 1.400 bp. Sementara amplikon gen GH American catfish (Rhamdia quelen) menggunakan primer Amc-GH-F dan Amc-GH-R sebesar 1.465 bp. Hasil analisis sekuensing gen penyandi GH menggunakan program BlastP dan Genetyx versi 7.0, menunjukkan bahwa sekuen gen penyandi GH lele dumbo memiliki homologi 80% dengan sekuen GH C. gariepinus pada bank gen (no. aksesi AF 416488.1), sehingga sebagian besar sekuen gen penyandi hormon pertumbuhan ikan tersebut dapat diamplifikasi secara in vitro.

Karakterisasi Karakter Fisiologi Genotipe-Genotipe F2 Padi Ketan dengan Kemampuan Recovery Setelah Infeksi Tungro: Karakterisasi Karakter Fisiologi Genotipe-Genotipe F2 Padi Ketan dengan Kemampuan Recovery Setelah Infeksi Tungro Ema Komalasari; Fitri Widiantini; Santika Sari; Nono Carsono
AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Vol 3 No 2 (2019): AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian
Publisher : Universitas Bangka Belitung

Tungro virus is one of the rice diseases which become one of limiting factors for rice production in Indonesia. The effective control can be done by using and rotation of resistant varieties. In order to develop tungro resistant varieties, hybridization has been conducted between susceptible (Ketonggo) and resistant variety (Utri Merah and ARC12596) i.e., Ketonggo x Utri Merah and Ketonggo x ARC12596. The main objective of the study was to characterize the physiological response of recovery genotypes group when compared to resistant genotypes. The genetic materials were F2 progenies of Ketonggo x Utri Merah and Ketonggo x ARC12596, each 230 genotypes. The experiment was conducted at BB Padi and Experimental Station of Faculty of Agriculture, Universitas Padjadjaran. Virus transmission was done using forced-tube inoculation method and symptoms scoring based on a standard evaluation system for rice. Traits observed were chlorophyll content, number of stomatal conductance, and quantum photosynthetic efficiency. The comparison of those traits between genotypes with recovery ability with those of resistant genotypes, susceptible genotypes, resistant variety check, and susceptible variety check was evaluated. It is found that recovered genotypes from both crossings did not show significant differences with those of resistant genotypes or resistant check variety on the above traits observed. Genotypes group with recovery ability can be used to suppress the spread of tungro disease.

Uji Daya Hasil dan Seleksi Famili Padi Generasi F4 dan F6 Hasil Persilangan Sintanur x PTB 33 dan Pandanwangi x PTB 33 di Jatinangor: Uji Daya Hasil dan Seleksi Famili Padi Generasi F4 dan F6 Hasil Persilangan Sintanur x PTB 33 dan Pandanwangi x PTB 33 di Jatinangor Zulfa Afifah; Nono Carsono; Santika Sari; Anas Anas
AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Vol 4 No 1 (2020): AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian
Publisher : Universitas Bangka Belitung

High yielding is one of main objectives on rice breeding. A preliminary yield trial is one of steps in the selection process of developing new varieties. Selection for yield and its component traits is a complex process due to the nature of quantitative traits. The right selection approach may improve the effectiveness of the selection. It is therefore the objective of the current study was to select high yielding rice genotypes. A total of 10 promising rice families were evaluated and compared to four check varieties. Least Significant Increase (LSI), and selection index (Mulamba & Mock and Genotype Ideotype Distance index) analysis were performed. LSI test showed that PP48-5 and SP101-3-1-5 had the best performance in almost all traits compared to the lowest average of the check variety. The selection index found that SP101-3-1-5 had highest yield selection index and was recognized as superior family by selection index. SP101-3-1-5 is selected as potential family to be developed as a new high yielding rice varieties

Gene Pyramiding for Brown Planthopper Resistance-related Traits, Early Maturity and Aroma of Rice Assisted by Molecular and Phenotypic Markers Nono Carsono; Riski Gusri Utami; Santika Sari; Noladhi Wicaksana
AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Vol 4 No 2 (2020): AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian
Publisher : Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/agrosainstek.v4i2.158

The brown planthopper (BPH)-resistant rice, early maturity, and aromatic are expected by both farmers and consumers. The traits have been combined through gene pyramiding and some promising rice genotypes obtained. However the genetic control of BPH resistance and maturity is quantitatively inherited, it is therefore both molecular and phenotypic assessments would be tremendously helpful in selecting promising genotypes. The study aimed to obtain genotypes with such valuable traits. Rice lines were analyzed using molecular markers i.e., RM586, RM589, RM8213 (BPH resistant gene markers); RM7610 and RM19414 (early maturity markers), and IFAP (Internal Fragrant Antisense Primer) for detecting aromatic, and INSP (Internal Non-fragrant Sense Primer) for non-aromatic rice. Phenotypic assessment was performed for brown planthopper resistant-related traits, such as chlorophyll content, stomatal conductance, and trichome density. Other evaluations were heading date and aroma (using 1.7% KOH solution). Results showed that molecular markers for evaluating BPH resistance genes (Bph3, Bph4, Qbph4, and Bph17), aroma (fgr gene), and heading date (Hd2 and Hd3 genes) could differentiate genotypes, and they serve as perfect markers, except for heading date markers. Seven genotypes i.e., #2, #3, #4, #5, #6, #10, and #11 were related to all traits expected based on molecular marker analysis. Meanwhile, genotypes #1, #2, #4, #6, and #11 were similar to their parents based on phenotypic analysis. Pyramiding program based on molecular and phenotypic markers enables us to combine three valuable traits into one rice genotype as presented in this study

Analisis Debit Banjir Sungai Cijangkelok Di Desa Cibingbin Kecamatan Cibingbin Kabupaten Kuningan Carsono, Nono
Syntax Literate Jurnal Ilmiah Indonesia
Publisher : CV. Ridwan Publisher

Penelitian ini bertujuan untuk menganalisis data hidrologi dan menganalisis debit banjir rencana pada Sungai Cijangkelok di Desa Cibingbin Kecamatan Cibingbin Kabupaten Kuningan. Metode Penelitian yang digunakan dalam penelitian ini adalah metode kuantitatif deskriftif. Penelitian kuantitatif sesuai dengan namanya banyak dituntut menggunakan angka, mulai dari pengumpulan data, penafsiran terhadap data tersebut, serta penampilan dan hasilnya. Demikian juga pemahaman akan kesimpulan penelitian disertai tabel, grafik, bagan, gambar atau tampilan lain. Analisis Hidrologi pada Sungai Cijangkelok dilakukan dengan analisis frekuensi mulai dari perhitungan curah hujan wilayah cara Poligon Thiessen sehingga diperoleh harga parameter statistik untuk perhitungan hujan rancangan yang dilakukan terhadap data curah hujan harian maksimum tahunan selama 15 tahun (tahun 2005 s.d. tahun 2019) dengan kala ulang 2 tahun, 5 tahun, 10 tahun, 25 tahun, 50 tahun, dan 100 tahun. Hasil perhitungan curah hujan rancangan kala ulang 5 tahun distribusi Gumbel sebesar 136,76 mm dan hasil distribusi Log Pearson Type III sebesar 127,83 mm. Dari hasil analisa frekuensi jenis distribusi yang memenuhi syarat yaitu distribusi Log Pearson Type III dengan nilai Cs = 0,753 selanjutnya dari hasil Uji keselarasan Smirnov-Kolmogorov dengan derajat signifikasi 5 % dan nilai ∆maks < ∆cr = 0,134 < 0,338., maka Metode Log Pearson Type III dapat digunakan untuk menghitung curah hujan periode ulang tertentu. Debit banjir rancangan kala ulang 5 tahun Metode Hidrograf Satuan Sintetik (HSS) Nakayasu sebesar 742,248 m3/det dengan intensitas curah hujan jam jaman metode Mononobe durasi 4 jam sebesar 17,587 mm/jam. Dengan kapasitas sungai yang didapat dari hasil perhitungan debit menggunakan metode mid section 239,30 m3/dt maka dapat disimpulkan bahwa Sungai Cijangkelok sudah tidak dapat menampung dan berpotensi banjir, dan perlu adanya normalisasi atau peninggian bangunan air dan atau tanggul pada Sungai Cijangkelok.

Correlations between morphological traits and catechin content on tea (Camellia sinensis (L.) O. Kuntze) Heri Syahrian; Nono Carsono; Neni Rostini; Vitria Puspitasari Rahadi; Bambang Sriyadi
Jurnal Penelitian Teh dan Kina Vol 18 No 1 (2015)
Publisher : Research Institute for Tea and Cinchona

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/pptk.jur.jptk.v18i1.64

Catechin content in tea plant is a major character in tea breeding program for a healthy beverage. A tea breeding program for high catechin content maybe will performed by indirect selecting a trait or traits that signiÂficantly correlated with high catechin content. This research was performed to identify morÂphological traits which maybe significantly correlated with high catechin content in tea plant. A randomized complete block design with three replications was used in this experiment using 10 selected clones. The experiment was performed in Gambung Experimental Station ot the Research Institute for Tea and Cinchona, Gambung, West Java. Catechin content, triÂchoma density of young leaves, weight of p+2, size of mother leaves, chlorophyll content of mother leaf, stomatal density of mother leaf, mother leaf thickness, and mother leaf angle, were measured. The results indicated that stomatal density and mother leaf angle were significantly correlated. Based on path analysis indirect selection for high catechin content could be performed by selecting stomatal density considering to trichoma density, mother leaf angle and chlorophyll content. In addition, indirect selection for high catechin content could be performed by selecting other leaf angle independently without considering other morphological traits.

Co-Authors Abd. Rasyid Syamsuri Ade Ismail Adilah Nurul Fitrah Afifah, Zulfa Agung Karuniawan Al Findy Yuhibba Fitriah Amalia Purdianty Amaliah, Lia Anas Anas Anas Anas, Anas Anas Zubair, Anas Anggita Dewi Ani Apriani Anita R. S. Wardani Anita Rachmasari S.W. Arif Solehudin Baehaki S.E. Baehaki S.E. Bambang Sriyadi Bambang Sriyadi, Bambang Betty Natalie Fitriatin Christian Bachem Christian Bachem, Christian Citra Bakti, Citra Danar Dono Dedi Ruswandi Desy Lidiasari Dewi, Atri Prautama Dwi Ariyani Eldikara, Ranggi Ema Komalasari Ema Komalasari, Ema Fachrunnisa, Laras Sitta Fadli Kurnia Farida Damayanti Feliatra Fitri Utami Hasan Fitri Widiantini Gatut Bayuaji Gigih Ibnu Prayoga Hasan, Fitri Utami Hasan, Fitri Utami Hera Hera Heri Syahrian Heri Syahrian, Heri Hersanti - Ibnu Dwi Buwono Ibrahim, Muhammad Yasser Inez Nur Indrayani Irma Mangatur Juniati, Atie Tri Kang, Seung Won kinya toriyama Leni Nurlaeli Irmayanti Lukman, Pradita N. M. Untung Kurnia Mahardhika, Dimas Kembara Mas Dadang Enjat Munajat Maulana, Haris Meddy Rachmadi Mitalo, Oscar W. Murdaningsih H. Karmana Nenet Susniahti Neni Rostini Neni Rostini Neni Rostini Nita Fitria Noladhi Wicaksana Nuryani Tinumbia, Nuryani Pradita N. Lukman Prayitno Prayitno Puji Syara Anggia Putra, Fitriansyah Rangga Jiwa Wibawa Ranggi Eldikara Reginawanti Hindersah Reisyi Rinola Tambunan Riski Gusri Utami Riski Gusri Utami Riski Gusri Utami S Imam Wahyudi Safitri, Adam Santika Sari Santika Sari Santika Sari Santika Sari Santika Sari Santika Sari Santika Sari Santika Sari Santika Sari Santika Sari Sari, Santika Sari, Santika SATRIYAS ILYAS Slamet, Whitea Yasmine Soedarsono Soedarsono Solehudin, Arif Sri Nurlianti Syindy Raffini Nasihin Tomohiko Yoshida Tomohiko Yoshida, Tomohiko Tri Joko Santoso Untung Susanto Untung Susanto Veithzal Rivai Zainal Vitria Puspitasari Rahadi Vitria Puspitasari Rahadi Wendy Wijaya Whitea Yasmine Slamet Wieny H. Rizky Yoana Saragih Yuniar Mulyani Zubair, Anas Zulfa Afifah

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