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All Journal HAYATI Journal of Biosciences Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Bios Logos BIOTROPIA - The Southeast Asian Journal of Tropical Biology AL KAUNIYAH Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Wacana, Jurnal Sosial dan Humaniora Proceeding International Conference on Global Resource Conservation Journal of Mathematical and Fundamental Sciences Jurnal Sumberdaya Hayati Bioeksperimen: Jurnal Penelitian Biologi Journal of Tropical Biodiversity and Biotechnology REINWARDTIA BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Biodjati Biosaintifika: Journal of Biology & Biology Education Forest and Society Floribunda Proceeding Biology Education Conference Makara Journal of Science Reinwardtia Jurnal Biosilampari: Jurnal Biologi
TATIK CHIKMAWATI
Bogor Agricultural University
Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Veterinary Other

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Journal : Makara Journal of Science

 1 
Leaf Trichome Morphology of Durio Kutejensis Landraces from Kalimantan Priyanti,; Chikmawati, Tatik; Sobir,; Hartana, Alex
Makara Journal of Science Vol. 19, No. 1
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The leaf trichome morphology of 19 Durio kutejensis (lai) landraces was studied. The observation of cross- and paradermal sections of D. kutejensis leaves showed that all landraces have glabrous leaves on the adaxial surface, while their abaxial surfaces are covered by six trichome types, one glandular (one- or two-celled stalks with a spheroid multicellular glandular head) and five non-glandular (complex peltate, simple peltate, cushioned stellate, flat stellate, and four-armed stellate trichome with a central cushion). All landraces were rarely covered by glandular trichomes. The non-glandular trichomes are varied in type, density, number of layers, diameter, and shape and margin color of the complex peltate among landraces. One landrace comprises three non-glandular trichome types, while the other landraces consist of four or five non-glandular trichome types. The shapes and margin color of complex peltate trichomes of D. kutejensis are the specific characteristics which distinguish this species from the other Durio species, however these characteristics cannot be used to differentiate one D. kutejensis landraces from the other. Therefore, other characteristics need to be explored in order to distinguish one D. kutejensis landraces from the other.
The Molecular Diversity-based ISSR of Durio tanjungpurensis Originating from West Kalimantan, Indonesia Riupassa, Pieter Agusthinus; Chikmawati, Tatik; Miftahudin,; Suharsono,
Makara Journal of Science Vol. 19, No. 1
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The Durian Tengkurak (Durio tanjungpurensis Navia) is one of the endangered exotic species in the Malvaceae family. The species is important for conservation of the germplasm and is considered a potential genetic resource for the development of durian in the future. The objective of this research project was to assess the molecular diversity of D. tanjungpurensis in West Kalimantan, based on Inter Simple Sequence Repeat (ISSR) markers. We applied ten ISSR primers to reveal the genetic diversity of 60 individuals from six natural endemic D. tanjungpurensis populations. The genetic diversity parameters were estimated based on binary data about PCR products (present or absent bands). The results showed that the mean number of observed alleles, the mean number of effective alleles, the genetic diversity, the Shannon’s Information Index score, the number of polymorphic loci, and the percentage of polymorphic loci were 1.53, 1.29, 0.17, 0.26, 77.83, and 52.59, respectively. An analysis of molecular variance (AMOVA) showed that the genetic diversity within a population (65%) was higher than that found between the populations (35%). UPGMA clustering and principal coordinate analysis, based on the DICE similarity matrix, were used to classify the populations into three groups: 1) Hutan Rejunak and Tembaga, 2) Bukit Merindang, and 3) Hutan Rawak, Bukit Sagu 1, and Bukit Sagu 2. Further analysis of the population structure using STRUCTURE software was used to classify all the individuals into two major categories, thus uniting Groups 2 and 3 as one major category. In conclusion, a high level of genetic diversity in the Durian Tengkurak was revealed utilizing the ISSR markers employed in the study.
Characteristics and Ideotype Formulation of Pulasan (Nephelium ramboutan-ake) Fruit Landrace from West Java, Indonesia Djuita, Nina Ratna; Hartana, Alex; Chikmawati, Tatik; Dorly,
Makara Journal of Science Vol. 21, No. 2
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Pulasan (Nephelium ramboutan-ake) is a unique tropical fruit, but its morphological variations have not been well characterized. This study was conducted to obtain data on the characteristics of pulasan landrace in West Java, Indonesia, and to formulate the ideotype of pulasan with superior characteristics, such as high fruit weight, sweet taste, and thick and easily peelable aril from the seed. Based on the descriptors of rambutan, 61 fruit variants were observed. The results showed that the average fruit weight was 46.0 g, with the highest at 103.5 g and the lowest at 23.1 g. The °Brix level variation of pulasan fruit was 16.8–29.6, and its vitamin C content was 14.0–24.0 mg/100 g. Five variants with superior fruit characters were identified among the sample collections used in the study
Phytochemical Composition of Selaginella spp. from Java Island Indonesia Chikmawati, Tatik; Setyawan, Ahmad Dwi; Miftahudin, Miftahudin
Makara Journal of Science Vol. 16, No. 2
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For a long time, people in West Java, Indonesia have used Selaginella as a traditional cure for several ailments including fever, minor wounds, broken bones, women’s health disorders or postnatal bleeding. However, information on the active compounds of the plant from Java Island has not been fully expounded. The objective of the research was to reveal the diversity of bioactive compounds and amentoflavone content of Selaginella from Java Island in order to optimize the use of this plant as a medicinal plant. Selaginella plants collected from 29 locations in Java were extracted and subjected to colorimetric and thin layer chromatography test to qualitatively analyze the bioactive compounds of alkaloid, phenol, and steroid. In addition, high performance liquid chromatography was performed to analyze the amentofalvone content of Selaginella extract. The research result showed that all the examined Selaginella species contained alkaloid, flavonoid, saponin, tannin, and steroid, but they did not contain hydroquinone. Seven of them contained the biflavonoid type of amentoflavone. The highest amentoflavone concentration, 6.87 ppm, was found in S. subalpina that originated from Gedung Songo, Central Java. Those results indicated that more than one Selaginella species originating from Java Island had marked potential for use as medicinal plants.
Gama Melon Parfum, a Potential Melon Genotype Tolerant of Salinity Stress Nugroho, Giri; Chikmawati, Tatik; Sartika, Dian; Yusuf, Adib Fakhruddin; Putro, Karso Suryo; Latifah, Vida Rahma; Daryono, Budi Setiadi; Wibowo, Anjar Tri; Nurhayati, Awik Puji Dyah; Meitha, Karlia; Miftahudin, Miftahudin
Makara Journal of Science Vol. 29, No. 3
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Melona, Tacapa Gold, and Gama Melon Parfum are novel Melon genotypes that have been introduced for cultivation in the saline-prone coastal region of Bocor Beach, Kebumen, Central Jawa. Elevated salt concentrations in the area pose challenges, impacting yield. The potential of these new genotypes for saline land remains unexplored. This study aimed to assess the morphophysiological and molecular responses of these genotypes to salinity stress. Seeds were germinated in cocopeat and ABmix 3000 solution and treated with 220 mM NaCl until harvest. The analysis included morphology, chlorophyll content, proline accumulation, malondialdehyde levels, and gene expression were analyzed. After 1 week of salinity stress, the results showed reduced plant height, leaf number, stomatal density, percentage of opened stomata, and width and length of leaves. The malondialdehyde, proline, and root-shoot dry mass ratio increased. The expression of CSD1-1, P5CR, and late embryogenesis abundant genes increased under salinity treatment. Gama Melon Parfum exhibits salt tolerance, as indicated by higher P5CR gene expression, proline accumulation, and leaf-relative water content. P5CR gene expression, proline accumulation, and leaf-relative water content are valuable indicators of salt tolerance for assessing melon genotypes.
Co-Authors Adhy Widya Setiawan ADI BASUKRIADI Adi Basukriadi Afri Irawan Afri Irawan, Afri Ahmad Dwi Setyawan Akrom, Akrom Alex Hartana Alex Hartana Alex Hartana ALEX HARTANA Alex Hartana Alex Hartana Alex Hartana Alex Hartana Alex Hartana Alex Hartana Alex Hartana Amelia Louisyane Puhili Amelia Luisyane Puhili Andayaningsih, Dwi Andi Madihah Manggabarani Anjar Tri Wibowo Anne Kusumawaty Anne Kusumawaty Arifin Surya Dwipa Irsyam Arifin Surya Dwipa Irsyam Asardi, Istiqoma Aswarina Nasution Awik Puji Dyah Nurhayati Badriah, Siti Nurul Billyardi Ramdhan Budi Prasetyo Budi Prasetyo BUDI SETIADI DARYONO Christyne SPLS Napitu Daniel Happy Putra Deby Arifiani Deby Arifiani, Deby Dede Setiadi Deden Girmansyah Dedy Darnaedi Dedy Darnaedi Dedy Darnaedi DEVI ALVITASARI Devi Alvitasari, Devi Devi Eka Lestari Dimas Prasaja, Dimas Dorly Dorly Dorly, Dwi Andayaningsih Dwi Putri Ramadhani Dwi Suci Setyaningsih Dwi Sunarti Puspitasari Dwi Sunarti Puspitasari, Dwi Sunarti Effendi, Surianto Eka Setiawan Eko Baroto Walujo EKO BAROTO WALUJO Eko Baroto Walujo Eko Baroto Waluyo Elizabeth A. Widjaja Elizabeth A. Widjaja Ervizal A.M Zuhud Ervizal Amir Muhammad Zuhud Ervizal AMZU Eva Kristinawati Putri Fadel Nugraha Furqon Al Muzakki Furqon Al Muzakki Gunawan Gunawan Gunawan Gunawan Haris Maulani HIMMAH RUSTIAMI Himmah Rustiami Himmah Rustiami Himmah Rustiami Himmah Rustiami, Himmah I Gusti Wayan Murjana Yasa I Putu Gede P. Damayanto I Putu Gede P. Damayanto I PUTU GEDE P. DAMAYANTO I PUTU GEDE P. DAMAYANTO, I PUTU GEDE P. Ibna Hayati IBNUL QAYIM Isna Arofatun Nikmah J PERRY GUSTAFSON Jati Batoro Jati Batoro Junaidi, Muhamad Khoon Meng Wong Kuswata Kartawinata KUSWATA KARTAWINATA Lana Maulana Latifah, Vida Rahma Manggabarani, Andi Madihah Marlina Ardiyani maulana, lana Meitha, Karlia Meliza, Rezika Mentari Putri Pratami Mentari Putri Pratami Mien A. Rifai Mien A. Rifai Miftahudin . Miftahudin Miftahudin Miftahudin Miftahudin Muchlis . Mugi Mumpuni Muhammad Efendi Muhammad Efendi Muhammad Efendi, Muhammad Muhammad Rifqi Hariri Muhammad Rifqi Hariri Muzzazinah Muzzazinah Muzzazinah Muzzazinah Nasrianti Syam NASRIANTI SYAM, NASRIANTI SYAM, NASRIANTI Nasution, Aswarina Nasution, Aswarina Nina Ratna Djuita Novi Novitasari, Risti Nugroho, Giri NUNIK SRI ARIYANTI Nurul Aini Nurul Aini Oktavia Pradikaningrum, Novi Peniwidiyanti Peniwidiyanti Pieter Agusthinus Riupassa Pranata, Syafroni Priyanti Priyanti Priyanti Priyanti Priyanti, Pudji Widodo Puhili, Amelia Luisyane Puspita Dewi Sopyati Putro, Karso Suryo Qothrunnada - Sungkar Rambe, Saidah Putri Ratna Djuita, Nina Rezika Meliza Ridha Mahyuni Ridha Mahyuni Ridha Mahyuni Ridha Mahyuni Risti Novitasari Rizki, Fitri Sri Rugayah Rugayah Rugayah Rugayah Rugayah Rugayah Rugayah Rugayah Rugayah Rugayah Safniyeti Safniyeti Safniyeti Safniyeti, Safniyeti Sartika, Dian Siti Halimatussadiah Sobir Sobir Sobir Sobir Sobir, Sri Endarti Rahayu Sri Endarti Rahayu, Sri Endarti Suharsono Suharsono Suharsono, Sulistijorini Sulistijorini Syadwina Hamama Dalimunthe Syadwina Hamama Dalimunthe Syafroni Pranata Tira Tahnia Titien Ngatinem Praptosuwiryo Titien Ngatinem Praptosuwiryo Titin Ngatinem Praptosuwiryo Tnunay, Ite Morina Yostianti Tri Atmowidi Wakhidah, Anisatu Z. WISNU HANDOYO ARDI WISNU HANDOYO ARDI, WISNU HANDOYO Y. Purwanto Y. Purwanto Yayan Wahyu Candra Kusuma Yohanes Purwanto Yusuf, Adib Fakhruddin Zulhendra zulhendra Zumaidar Zumaidar